Study of Thermostable Chitinase Enzymes from Indonesian Bacillus K29-14
Thermophilic microorganisms capable of producing chitinase enzymes were screened from samples collected from several crater and geothermal areas. The chitinolytic microorganisms were grown in a selective medium containing colloidal chitin. The Bacillus K29-14 isolate was found to exhibit the highest...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2004-08, Vol.14 (4), p.647-652 |
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| creator | Sri, Rahayu (Bogor Agricultural University, Bogor, West Java, Indonesia), E-mail: mthenawidjaja@yahoo.com Hwang, J.K. (Yonsei University, Seoul, Republic of Korea) Tanuwidjaja, Fredy (Bogor Agricultural University, Bogor, West Java, Indonesia) Rukayadi, Yaya (Bogor Agricultural University, Bogor, West Java, Indonesia) Suwanto, Antonius (Bogor Agricultural University, Bogor, West Java, Indonesia) Suhartono, Maggy Thenawidjaja (Bogor Agricultural University, Bogor, West Java, Indonesia) Pyun, Y.R. (Yonsei University, Seoul, Republic of Korea) |
| description | Thermophilic microorganisms capable of producing chitinase enzymes were screened from samples collected from several crater and geothermal areas. The chitinolytic microorganisms were grown in a selective medium containing colloidal chitin. The Bacillus K29-14 isolate was found to exhibit the highest chitinase and chitin deacetylase activities. When grown in a chitin-containing medium, the isolate produced extracellular chitinase after 24 h of incubation. The optimum temperature and pH for the chitinase were 55℃ and pH 7, respectively, while those for the chitin deacetylase were 55℃ and pH 8, respectively. |
| format | Article |
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(Yonsei University, Seoul, Republic of Korea) ; Tanuwidjaja, Fredy (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Rukayadi, Yaya (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Suwanto, Antonius (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Suhartono, Maggy Thenawidjaja (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Pyun, Y.R. (Yonsei University, Seoul, Republic of Korea)</creator><creatorcontrib>Sri, Rahayu (Bogor Agricultural University, Bogor, West Java, Indonesia), E-mail: mthenawidjaja@yahoo.com ; Hwang, J.K. (Yonsei University, Seoul, Republic of Korea) ; Tanuwidjaja, Fredy (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Rukayadi, Yaya (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Suwanto, Antonius (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Suhartono, Maggy Thenawidjaja (Bogor Agricultural University, Bogor, West Java, Indonesia) ; Pyun, Y.R. (Yonsei University, Seoul, Republic of Korea)</creatorcontrib><description>Thermophilic microorganisms capable of producing chitinase enzymes were screened from samples collected from several crater and geothermal areas. The chitinolytic microorganisms were grown in a selective medium containing colloidal chitin. The Bacillus K29-14 isolate was found to exhibit the highest chitinase and chitin deacetylase activities. When grown in a chitin-containing medium, the isolate produced extracellular chitinase after 24 h of incubation. The optimum temperature and pH for the chitinase were 55℃ and pH 7, respectively, while those for the chitin deacetylase were 55℃ and pH 8, respectively.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>Biological and medical sciences ; Biotechnology ; chitin deacetylase ; CHITINASE ; ESTABILIDAD ; Fundamental and applied biological sciences. 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The Bacillus K29-14 isolate was found to exhibit the highest chitinase and chitin deacetylase activities. When grown in a chitin-containing medium, the isolate produced extracellular chitinase after 24 h of incubation. The optimum temperature and pH for the chitinase were 55℃ and pH 7, respectively, while those for the chitin deacetylase were 55℃ and pH 8, respectively.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>chitin deacetylase</subject><subject>CHITINASE</subject><subject>ESTABILIDAD</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>MICROORGANISME</subject><subject>MICROORGANISMOS</subject><subject>MICROORGANISMS</subject><subject>QUITINASA</subject><subject>STABILITE</subject><subject>STABILITY</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNotzE9LwzAYgPEeFJzTjyDk4rHwvkmTtkctc44NBJ3n8jZ_XKRNR9Id6qd3sJ2ey4_nJlsgYJmXFZd32X1KvwAKeaUW2fprOpmZjY7tDzYOY5qo6y1rDn7ygZJlq_A3DzYxF8eBbYIZg02eAnsl7fv-lNiW1zkWD9mtoz7Zx2uX2ffbat-857uP9aZ52eWOg5xyZ6iryZTgdAe6Nug0t6V2UBilBHFjURNyVWFBTmqpO2GFQSOspIKLWiyz58v3SElT7yIF7VN7jH6gOLeoQBVKVmf3dHGOxpZ-4tlsPzmABORQovgHh2BQ2A</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Sri, Rahayu (Bogor Agricultural University, Bogor, West Java, Indonesia), E-mail: mthenawidjaja@yahoo.com</creator><creator>Hwang, J.K. 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| fulltext | fulltext |
| identifier | ISSN: 1017-7825 |
| ispartof | Journal of microbiology and biotechnology, 2004-08, Vol.14 (4), p.647-652 |
| issn | 1017-7825 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_16064658 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Biotechnology chitin deacetylase CHITINASE ESTABILIDAD Fundamental and applied biological sciences. Psychology MICROORGANISME MICROORGANISMOS MICROORGANISMS QUITINASA STABILITE STABILITY |
| title | Study of Thermostable Chitinase Enzymes from Indonesian Bacillus K29-14 |
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