Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism
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Veröffentlicht in: | Applied and Environmental Microbiology 2004-06, Vol.70 (6), p.3618-3623 |
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creator | Fey, Axel Eichler, Stefan Flavier, Sébastien Christen, Richard Höfle, Manfred G. Guzmán, Carlos A. |
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doi_str_mv | 10.1128/AEM.70.6.3618-3623.2004 |
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</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.70.6.3618-3623.2004</identifier><identifier>PMID: 15184165</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacteria ; Bacterial Proteins - genetics ; Biological and medical sciences ; Colony Count, Microbial ; DNA Primers ; DNA, Bacterial - genetics ; DNA, Bacterial - standards ; Environmental Monitoring - methods ; Eukaryotes ; Fresh Water - microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Methods ; Microbiology ; Polymerase Chain Reaction - methods ; Polymerase Chain Reaction - standards ; Quantitative genetics ; RNA, Bacterial - genetics ; RNA, Bacterial - standards ; RNA, Ribosomal, 16S - genetics ; Salmonella ; Salmonella typhimurium - isolation & purification ; Sensitivity and Specificity ; Test methods</subject><ispartof>Applied and Environmental Microbiology, 2004-06, Vol.70 (6), p.3618-3623</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Jun 2004</rights><rights>Copyright © 2004, American Society for Microbiology 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c611t-9d3568531852686edabf164afe0a2589108a9a88e6b78b6d8b6dc6f1f566f57a3</citedby><cites>FETCH-LOGICAL-c611t-9d3568531852686edabf164afe0a2589108a9a88e6b78b6d8b6dc6f1f566f57a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC427797/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC427797/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3186,3187,27922,27923,53789,53791</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15860420$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15184165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fey, Axel</creatorcontrib><creatorcontrib>Eichler, Stefan</creatorcontrib><creatorcontrib>Flavier, Sébastien</creatorcontrib><creatorcontrib>Christen, Richard</creatorcontrib><creatorcontrib>Höfle, Manfred G.</creatorcontrib><creatorcontrib>Guzmán, Carlos A.</creatorcontrib><title>Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Classifications
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</description><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>DNA Primers</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - standards</subject><subject>Environmental Monitoring - methods</subject><subject>Eukaryotes</subject><subject>Fresh Water - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Methods</subject><subject>Microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymerase Chain Reaction - standards</subject><subject>Quantitative genetics</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Bacterial - standards</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Salmonella</subject><subject>Salmonella typhimurium - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>Test methods</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksuO0zAUhiMEYsrAK4BBghUpdhxfsphFpioXaYaB0hFL69RxWo8Su9gJiOfghXHUihnYsLC88Pf7XPRl2TOC54QU8k29vJwLPOdzyonMKS_ovMC4vJfNCK5kzijl97MZxlWVF0WJT7JHMd7gRGAuH2YnhBFZEs5m2a9lHGDT2bjrjRuQbxGglYEuX9veoE-LVX4O0TSo3u-DB71DrQ-o1noMMBj0eQQ32NZqGKx3U_oc9GCChQ6tPtZoDWFrhoisQ18TH16j62jdFn2BrvfOdB0giKnipW9Mh67CFpyN_ePsQQtdNE-O92l2_Xa5XrzPL67efVjUF7nmhAx51VDGJaNEsoJLbhrYtISX0BoMBZMVwRIqkNLwjZAb3kxH85a0jPOWCaCn2dnh3_246U2j0wICdGofbA_hp_Jg1d8vzu7U1n9XZSFEJVL-1TEf_LfRxEH1NuppKmf8GBURlKVG5P_BUmAsSpbAF_-AN34MLi1BFZhVHBOKEyQOkA4-xmDaPx0TrCY7VLJDCay4muxQkx1qsiMln94d-DZ31CEBL48ARA1dG8BpG-9wkuOymFp4fuB2drv7YYNREHsFpr8tS38D3ODOfw</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Fey, Axel</creator><creator>Eichler, Stefan</creator><creator>Flavier, Sébastien</creator><creator>Christen, Richard</creator><creator>Höfle, Manfred G.</creator><creator>Guzmán, Carlos A.</creator><general>American Society for Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>20040601</creationdate><title>Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism</title><author>Fey, Axel ; Eichler, Stefan ; Flavier, Sébastien ; Christen, Richard ; Höfle, Manfred G. ; Guzmán, Carlos A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c611t-9d3568531852686edabf164afe0a2589108a9a88e6b78b6d8b6dc6f1f566f57a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Colony Count, Microbial</topic><topic>DNA Primers</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - standards</topic><topic>Environmental Monitoring - methods</topic><topic>Eukaryotes</topic><topic>Fresh Water - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Methods</topic><topic>Microbiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymerase Chain Reaction - standards</topic><topic>Quantitative genetics</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Bacterial - standards</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Salmonella</topic><topic>Salmonella typhimurium - isolation & purification</topic><topic>Sensitivity and Specificity</topic><topic>Test methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fey, Axel</creatorcontrib><creatorcontrib>Eichler, Stefan</creatorcontrib><creatorcontrib>Flavier, Sébastien</creatorcontrib><creatorcontrib>Christen, Richard</creatorcontrib><creatorcontrib>Höfle, Manfred G.</creatorcontrib><creatorcontrib>Guzmán, Carlos A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fey, Axel</au><au>Eichler, Stefan</au><au>Flavier, Sébastien</au><au>Christen, Richard</au><au>Höfle, Manfred G.</au><au>Guzmán, Carlos A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>70</volume><issue>6</issue><spage>3618</spage><epage>3623</epage><pages>3618-3623</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Classifications
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</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>15184165</pmid><doi>10.1128/AEM.70.6.3618-3623.2004</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Bacteria Bacterial Proteins - genetics Biological and medical sciences Colony Count, Microbial DNA Primers DNA, Bacterial - genetics DNA, Bacterial - standards Environmental Monitoring - methods Eukaryotes Fresh Water - microbiology Fundamental and applied biological sciences. Psychology Humans Methods Microbiology Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Quantitative genetics RNA, Bacterial - genetics RNA, Bacterial - standards RNA, Ribosomal, 16S - genetics Salmonella Salmonella typhimurium - isolation & purification Sensitivity and Specificity Test methods |
title | Establishment of a Real-Time PCR-Based Approach for Accurate Quantification of Bacterial RNA Targets in Water, Using Salmonella as a Model Organism |
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