Induction of the T7 Promoter Using Lactose for Production of Recombinant Plasminogen Kringle 1-3 in Escherichia coli

A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer co...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2004-04, Vol.14 (2), p.225-230
Hauptverfasser:	Lim, H.K. (Mogam Biotechnology Institute, Yongin, Republic of Korea), Seo, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: jhseo94@snu.ac.kr, Lee, S.U. (Mogam Biotechnology Institute, Yongin, Republic of Korea), Chung, S.I. (Mogam Biotechnology Institute, Yongin, Republic of Korea), Jung, K.H. (Mogam Biotechnology Institute, Yongin, Republic of Korea)
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology ESCHERICHIA COLI Fundamental and applied biological sciences. Psychology induction LACTOSA LACTOSE rK1-3 T7 promoter
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