Induction of the T7 Promoter Using Lactose for Production of Recombinant Plasminogen Kringle 1-3 in Escherichia coli
A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer co...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2004-04, Vol.14 (2), p.225-230 |
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| creator | Lim, H.K. (Mogam Biotechnology Institute, Yongin, Republic of Korea) Seo, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: jhseo94@snu.ac.kr Lee, S.U. (Mogam Biotechnology Institute, Yongin, Republic of Korea) Chung, S.I. (Mogam Biotechnology Institute, Yongin, Republic of Korea) Jung, K.H. (Mogam Biotechnology Institute, Yongin, Republic of Korea) |
| description | A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer concentration in various culture conditions including batch and high-cell-density fed-batch cultures. In the fed-batch culture, the induction around 6 h after initiation of the DO-stat fed-batch culture resulted in the highest expression level of rKI-3 among the induction points examined. |
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(Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Seo, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: jhseo94@snu.ac.kr ; Lee, S.U. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Chung, S.I. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Jung, K.H. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creator><creatorcontrib>Lim, H.K. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Seo, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: jhseo94@snu.ac.kr ; Lee, S.U. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Chung, S.I. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Jung, K.H. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creatorcontrib><description>A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer concentration in various culture conditions including batch and high-cell-density fed-batch cultures. In the fed-batch culture, the induction around 6 h after initiation of the DO-stat fed-batch culture resulted in the highest expression level of rKI-3 among the induction points examined.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>Biological and medical sciences ; Biotechnology ; ESCHERICHIA COLI ; Fundamental and applied biological sciences. Psychology ; induction ; LACTOSA ; LACTOSE ; rK1-3 ; T7 promoter</subject><ispartof>Journal of microbiology and biotechnology, 2004-04, Vol.14 (2), p.225-230</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15698249$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Lim, H.K. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creatorcontrib><creatorcontrib>Seo, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: jhseo94@snu.ac.kr</creatorcontrib><creatorcontrib>Lee, S.U. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creatorcontrib><creatorcontrib>Chung, S.I. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creatorcontrib><creatorcontrib>Jung, K.H. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creatorcontrib><title>Induction of the T7 Promoter Using Lactose for Production of Recombinant Plasminogen Kringle 1-3 in Escherichia coli</title><title>Journal of microbiology and biotechnology</title><description>A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer concentration in various culture conditions including batch and high-cell-density fed-batch cultures. In the fed-batch culture, the induction around 6 h after initiation of the DO-stat fed-batch culture resulted in the highest expression level of rKI-3 among the induction points examined.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>ESCHERICHIA COLI</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>induction</subject><subject>LACTOSA</subject><subject>LACTOSE</subject><subject>rK1-3</subject><subject>T7 promoter</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpNjc1OwzAQhHMAiVJ4BKS9cIzknzhxjqgqUDUSVdWeI8dZJ0aJXdnmwNsTVA5cZlaab3ZushUltMorycRddh_jJyElZbJcZWnn-i-drHfgDaQR4VTBIfjZJwxwjtYN0CidfEQwPvxG__gjaj931imX4DCpOFvnB3SwD0tvQqA5B-tgG_WIwerRKtB-sg_ZrVFTxMc_X2fn1-1p8543H2-7zUuTG0ZEylnFBKEGSy4pI5xIIepO1qJj3GBtioKVBRdVj0Yvlyx7UTAsUCpNkFHR83X2fP17UVGryQTltI3tJdhZhe-WirKWrKgX7unKGeVbNYSF2R8ZIcv6opz_AHiEXt0</recordid><startdate>20040401</startdate><enddate>20040401</enddate><creator>Lim, H.K. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creator><creator>Seo, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: jhseo94@snu.ac.kr</creator><creator>Lee, S.U. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creator><creator>Chung, S.I. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creator><creator>Jung, K.H. (Mogam Biotechnology Institute, Yongin, Republic of Korea)</creator><general>Korean Society for Applied Microbiology</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>20040401</creationdate><title>Induction of the T7 Promoter Using Lactose for Production of Recombinant Plasminogen Kringle 1-3 in Escherichia coli</title><author>Lim, H.K. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Seo, J.H. (Seoul National University, Seoul, Republic of Korea), E-mail: jhseo94@snu.ac.kr ; Lee, S.U. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Chung, S.I. (Mogam Biotechnology Institute, Yongin, Republic of Korea) ; Jung, K.H. 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(Mogam Biotechnology Institute, Yongin, Republic of Korea)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of the T7 Promoter Using Lactose for Production of Recombinant Plasminogen Kringle 1-3 in Escherichia coli</atitle><jtitle>Journal of microbiology and biotechnology</jtitle><date>2004-04-01</date><risdate>2004</risdate><volume>14</volume><issue>2</issue><spage>225</spage><epage>230</epage><pages>225-230</pages><issn>1017-7825</issn><abstract>A plasminogen kringle domain 1 to 3, rKl-3, was expressed in Escherichia coli under the control of T7 promoter. For the cost-effective production of rKl-3, the induction process was analyzed and optimized. Induction characteristics with lactose were analyzed in terms of induction time and inducer concentration in various culture conditions including batch and high-cell-density fed-batch cultures. In the fed-batch culture, the induction around 6 h after initiation of the DO-stat fed-batch culture resulted in the highest expression level of rKI-3 among the induction points examined.</abstract><cop>Seoul</cop><pub>Korean Society for Applied Microbiology</pub><tpages>6</tpages></addata></record> |
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| ispartof | Journal of microbiology and biotechnology, 2004-04, Vol.14 (2), p.225-230 |
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| subjects | Biological and medical sciences Biotechnology ESCHERICHIA COLI Fundamental and applied biological sciences. Psychology induction LACTOSA LACTOSE rK1-3 T7 promoter |
| title | Induction of the T7 Promoter Using Lactose for Production of Recombinant Plasminogen Kringle 1-3 in Escherichia coli |
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