Optimization and Validation of a Virus Filtration Process for Efficient Removal of Viruses from Urokinase Solution Prepared from Human Urine
Urokinase is an enzyme with fibrinolytic activity (plasminogen activator) isolated from fresh urine of healthy men. Viral safety is an important prerequisite for clinical preparation of the protein from urine. In order to increase the viral safety of a high purity urokinase in regard to non-envelope...
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| Veröffentlicht in: | Journal of microbiology and biotechnology 2004-02, Vol.14 (1), p.140-147 |
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| container_title | Journal of microbiology and biotechnology |
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| creator | Kim, I.S. (Hannam University, Daejeon, Republic of Korea), E-mail: inskim@hannam.alc.kr Choi, Y.W. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea) Lee, S.R. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea) |
| description | Urokinase is an enzyme with fibrinolytic activity (plasminogen activator) isolated from fresh urine of healthy men. Viral safety is an important prerequisite for clinical preparation of the protein from urine. In order to increase the viral safety of a high purity urokinase in regard to non-enveloped viruses, a virus removal process using a novel polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. Urokinase was able to pass through the filter with recoveries of 95% in the production scale process. |
| format | Article |
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(Hannam University, Daejeon, Republic of Korea), E-mail: inskim@hannam.alc.kr ; Choi, Y.W. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea) ; Lee, S.R. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</creator><creatorcontrib>Kim, I.S. (Hannam University, Daejeon, Republic of Korea), E-mail: inskim@hannam.alc.kr ; Choi, Y.W. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea) ; Lee, S.R. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</creatorcontrib><description>Urokinase is an enzyme with fibrinolytic activity (plasminogen activator) isolated from fresh urine of healthy men. Viral safety is an important prerequisite for clinical preparation of the protein from urine. In order to increase the viral safety of a high purity urokinase in regard to non-enveloped viruses, a virus removal process using a novel polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. Urokinase was able to pass through the filter with recoveries of 95% in the production scale process.</description><identifier>ISSN: 1017-7825</identifier><language>eng</language><publisher>Seoul: Korean Society for Applied Microbiology</publisher><subject>ACTIVADOR DE PLASMINOGENO ; ACTIVATEUR DE PLASMINOGENE ; Biological and medical sciences ; Biotechnology ; Fundamental and applied biological sciences. 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(Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</creatorcontrib><title>Optimization and Validation of a Virus Filtration Process for Efficient Removal of Viruses from Urokinase Solution Prepared from Human Urine</title><title>Journal of microbiology and biotechnology</title><description>Urokinase is an enzyme with fibrinolytic activity (plasminogen activator) isolated from fresh urine of healthy men. Viral safety is an important prerequisite for clinical preparation of the protein from urine. In order to increase the viral safety of a high purity urokinase in regard to non-enveloped viruses, a virus removal process using a novel polyvinylidene fluoride membrane filter (Viresolve NFP) has been optimized. Urokinase was able to pass through the filter with recoveries of 95% in the production scale process.</description><subject>ACTIVADOR DE PLASMINOGENO</subject><subject>ACTIVATEUR DE PLASMINOGENE</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hepatitis A virus</subject><subject>parvovirus</subject><subject>PLASMINOGEN ACTIVATOR</subject><subject>validation</subject><subject>VIRUS</subject><subject>virus filtration</subject><subject>virus removal</subject><subject>VIRUSES</subject><issn>1017-7825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNotzcFOwzAMBuAcQGIMHgEpF46VkrRN2yOaNoaYNDTYrpWbOCjQJlXSIcEz8NB0dCfL9uffF2TGGS-SohT5FbmO8YMxyUUpZ-R32w-2sz8wWO8oOE0P0Fo9td5QoAcbjpGubDuEafoSvMIYqfGBLo2xyqIb6A47_wXt6eb_AkcQfEf3wX9aBxHpq2-P5wDsIaCewPrYgRuZdXhDLg20EW_PdU72q-XbYp1sto9Pi4dNYgTLh6QUQjZKIphMA0qGJlO65Gg001ClKpNcMq0KXRmecRwXjU4VyIKhbipZpHNyP-X2EBW0JoBTNtZ9sB2E75rn-fiHn9zd5Az4Gt7DaJ53grGccV6VMv0D9vprsQ</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Kim, I.S. (Hannam University, Daejeon, Republic of Korea), E-mail: inskim@hannam.alc.kr</creator><creator>Choi, Y.W. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</creator><creator>Lee, S.R. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</creator><general>Korean Society for Applied Microbiology</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>20040201</creationdate><title>Optimization and Validation of a Virus Filtration Process for Efficient Removal of Viruses from Urokinase Solution Prepared from Human Urine</title><author>Kim, I.S. (Hannam University, Daejeon, Republic of Korea), E-mail: inskim@hannam.alc.kr ; Choi, Y.W. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea) ; Lee, S.R. 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(Hannam University, Daejeon, Republic of Korea), E-mail: inskim@hannam.alc.kr</creatorcontrib><creatorcontrib>Choi, Y.W. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</creatorcontrib><creatorcontrib>Lee, S.R. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Journal of microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, I.S. (Hannam University, Daejeon, Republic of Korea), E-mail: inskim@hannam.alc.kr</au><au>Choi, Y.W. (Green Cross Plasma Derivatives Company, Yongin, Republic of Korea)</au><au>Lee, S.R. 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| ispartof | Journal of microbiology and biotechnology, 2004-02, Vol.14 (1), p.140-147 |
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| language | eng |
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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | ACTIVADOR DE PLASMINOGENO ACTIVATEUR DE PLASMINOGENE Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology hepatitis A virus parvovirus PLASMINOGEN ACTIVATOR validation VIRUS virus filtration virus removal VIRUSES |
| title | Optimization and Validation of a Virus Filtration Process for Efficient Removal of Viruses from Urokinase Solution Prepared from Human Urine |
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