Characterization of streptozotocin-induced diabetic rats and pharmacodynamics of insulin formulations
Morphological and functional changes of rat pancreatic islets caused by administration of streptozotocin (STZ) and the bioavailability of insulin formulations administered to STZ-induced diabetic rats with fasting (12 h) or non-fasting were investigated. Islets isolated from normal rats maintained a...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-11, Vol.67 (11), p.2396-2401 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Lee, J.J. (Korea Univ., Seoul (Korea R.)) Yi, H.Y Yang, J.W Shin, J.S Kwon, J.H Kim, C.W |
| description | Morphological and functional changes of rat pancreatic islets caused by administration of streptozotocin (STZ) and the bioavailability of insulin formulations administered to STZ-induced diabetic rats with fasting (12 h) or non-fasting were investigated. Islets isolated from normal rats maintained a good three-dimensional structure and the islet yield was 962.5 =+- 86.5 islet equivalent number (IEQ, islets converted to an average diameter of 150 micro m). In the diabetic group (>500 mg/ ml blood glucose), the islet yield was only 44.4 =+- 8.3 IEQ and the islet was severely damaged. The minimum reduction of blood glucose of each formulation, such as insulin solution, microcrystal, and insulin microcrystal capsule, was shown to be 11.3, 11.0, and 16.3 mg/dl, respectively, at 6 h in fasting with diabetic rats. These results indicated that the administration of insulin formulations to the fasting groups increased the severe hypoglycemic effect of insulin action more than in non-fasting diabetic rats. The diabetic rat with fasting has a regulatory disorder in maintaining the blood glucose level. Accordingly, the validity of pharmacological availability as an optimal modeling of insulin formulations is best in non-fasting STZ-induced diabetic rats. |
| doi_str_mv | 10.1271/bbb.67.2396 |
| format | Article |
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(Korea Univ., Seoul (Korea R.)) ; Yi, H.Y ; Yang, J.W ; Shin, J.S ; Kwon, J.H ; Kim, C.W</creator><creatorcontrib>Lee, J.J. (Korea Univ., Seoul (Korea R.)) ; Yi, H.Y ; Yang, J.W ; Shin, J.S ; Kwon, J.H ; Kim, C.W</creatorcontrib><description>Morphological and functional changes of rat pancreatic islets caused by administration of streptozotocin (STZ) and the bioavailability of insulin formulations administered to STZ-induced diabetic rats with fasting (12 h) or non-fasting were investigated. Islets isolated from normal rats maintained a good three-dimensional structure and the islet yield was 962.5 =+- 86.5 islet equivalent number (IEQ, islets converted to an average diameter of 150 micro m). In the diabetic group (>500 mg/ ml blood glucose), the islet yield was only 44.4 =+- 8.3 IEQ and the islet was severely damaged. The minimum reduction of blood glucose of each formulation, such as insulin solution, microcrystal, and insulin microcrystal capsule, was shown to be 11.3, 11.0, and 16.3 mg/dl, respectively, at 6 h in fasting with diabetic rats. These results indicated that the administration of insulin formulations to the fasting groups increased the severe hypoglycemic effect of insulin action more than in non-fasting diabetic rats. The diabetic rat with fasting has a regulatory disorder in maintaining the blood glucose level. Accordingly, the validity of pharmacological availability as an optimal modeling of insulin formulations is best in non-fasting STZ-induced diabetic rats.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.67.2396</identifier><identifier>PMID: 14646199</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Animals ; ANTIBIOTICS ; Biological and medical sciences ; Blood Glucose - drug effects ; Blood Glucose - metabolism ; Cell Separation ; DIABETES ; Diabetes Mellitus, Experimental - metabolism ; Diabetes Mellitus, Experimental - pathology ; Fundamental and applied biological sciences. Psychology ; Glucose Tolerance Test ; Hypoglycemic Agents - pharmacokinetics ; Hypoglycemic Agents - therapeutic use ; INSULIN ; Insulin - pharmacokinetics ; Insulin - therapeutic use ; islet ; Islets of Langerhans - pathology ; Islets of Langerhans - secretion ; Kinetics ; Male ; MANKIND ; Pancreas - pathology ; pharmacodynamics ; PHARMACOLOGY ; Rats ; Rats, Sprague-Dawley ; streptozotocin</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2003-11, Vol.67 (11), p.2396-2401</ispartof><rights>2003 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2003</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c665t-60598b671383362c0267688f268815dd181e099a5bcd4fd64fda6542a89429b93</citedby><cites>FETCH-LOGICAL-c665t-60598b671383362c0267688f268815dd181e099a5bcd4fd64fda6542a89429b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15405204$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14646199$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, J.J. (Korea Univ., Seoul (Korea R.))</creatorcontrib><creatorcontrib>Yi, H.Y</creatorcontrib><creatorcontrib>Yang, J.W</creatorcontrib><creatorcontrib>Shin, J.S</creatorcontrib><creatorcontrib>Kwon, J.H</creatorcontrib><creatorcontrib>Kim, C.W</creatorcontrib><title>Characterization of streptozotocin-induced diabetic rats and pharmacodynamics of insulin formulations</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Morphological and functional changes of rat pancreatic islets caused by administration of streptozotocin (STZ) and the bioavailability of insulin formulations administered to STZ-induced diabetic rats with fasting (12 h) or non-fasting were investigated. Islets isolated from normal rats maintained a good three-dimensional structure and the islet yield was 962.5 =+- 86.5 islet equivalent number (IEQ, islets converted to an average diameter of 150 micro m). In the diabetic group (>500 mg/ ml blood glucose), the islet yield was only 44.4 =+- 8.3 IEQ and the islet was severely damaged. The minimum reduction of blood glucose of each formulation, such as insulin solution, microcrystal, and insulin microcrystal capsule, was shown to be 11.3, 11.0, and 16.3 mg/dl, respectively, at 6 h in fasting with diabetic rats. These results indicated that the administration of insulin formulations to the fasting groups increased the severe hypoglycemic effect of insulin action more than in non-fasting diabetic rats. The diabetic rat with fasting has a regulatory disorder in maintaining the blood glucose level. Accordingly, the validity of pharmacological availability as an optimal modeling of insulin formulations is best in non-fasting STZ-induced diabetic rats.</description><subject>Animals</subject><subject>ANTIBIOTICS</subject><subject>Biological and medical sciences</subject><subject>Blood Glucose - drug effects</subject><subject>Blood Glucose - metabolism</subject><subject>Cell Separation</subject><subject>DIABETES</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose Tolerance Test</subject><subject>Hypoglycemic Agents - pharmacokinetics</subject><subject>Hypoglycemic Agents - therapeutic use</subject><subject>INSULIN</subject><subject>Insulin - pharmacokinetics</subject><subject>Insulin - therapeutic use</subject><subject>islet</subject><subject>Islets of Langerhans - pathology</subject><subject>Islets of Langerhans - secretion</subject><subject>Kinetics</subject><subject>Male</subject><subject>MANKIND</subject><subject>Pancreas - pathology</subject><subject>pharmacodynamics</subject><subject>PHARMACOLOGY</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>streptozotocin</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M1rHCEYBnApCc027annloHQXsps1VFnPIYl_QiB9JCc5R11WsOMbtQhbP76ON0tgZKDCvJ7H14ehN4TvCa0JV_7vl-Ldk0bKV6hFWlYWwvJ2iO0wpKIumOcnKA3Kd1hXD44eY1OCBNMEClXyG7-QASdbXSPkF3wVRiqlKPd5vAYctDO186bWVtTGQe9zU5XEXKqwJtqW4Yn0MHsPExOp2XY-TSPzldDiNM8_s1Mb9HxAGOy7w7vKbr9dnGz-VFfXX__uTm_qrUQPNcCc9n1oiVN1zSCakxFK7puoOUi3BjSEYulBN5rwwYjygHBGYVOMip72Zyiz_vcbQz3s01ZTS5pO47gbZiTagmjtCQUePYfvAtz9GU3RRiTjBe0qC97pWNIKdpBbaObIO4UwWrpXpXulWjV0n3RHw-Zcz9Z82wPZRfw6QAgaRiHCF679Ow4w5xiVpzYO-eXEuEhxNGoDLsxxH9DzcsbfNgPDhAU_I7FXf6iGDOMywpd8wStzKfs</recordid><startdate>20031101</startdate><enddate>20031101</enddate><creator>Lee, J.J. (Korea Univ., Seoul (Korea R.))</creator><creator>Yi, H.Y</creator><creator>Yang, J.W</creator><creator>Shin, J.S</creator><creator>Kwon, J.H</creator><creator>Kim, C.W</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20031101</creationdate><title>Characterization of streptozotocin-induced diabetic rats and pharmacodynamics of insulin formulations</title><author>Lee, J.J. (Korea Univ., Seoul (Korea R.)) ; Yi, H.Y ; Yang, J.W ; Shin, J.S ; Kwon, J.H ; Kim, C.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c665t-60598b671383362c0267688f268815dd181e099a5bcd4fd64fda6542a89429b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>ANTIBIOTICS</topic><topic>Biological and medical sciences</topic><topic>Blood Glucose - drug effects</topic><topic>Blood Glucose - metabolism</topic><topic>Cell Separation</topic><topic>DIABETES</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose Tolerance Test</topic><topic>Hypoglycemic Agents - pharmacokinetics</topic><topic>Hypoglycemic Agents - therapeutic use</topic><topic>INSULIN</topic><topic>Insulin - pharmacokinetics</topic><topic>Insulin - therapeutic use</topic><topic>islet</topic><topic>Islets of Langerhans - pathology</topic><topic>Islets of Langerhans - secretion</topic><topic>Kinetics</topic><topic>Male</topic><topic>MANKIND</topic><topic>Pancreas - pathology</topic><topic>pharmacodynamics</topic><topic>PHARMACOLOGY</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>streptozotocin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, J.J. (Korea Univ., Seoul (Korea R.))</creatorcontrib><creatorcontrib>Yi, H.Y</creatorcontrib><creatorcontrib>Yang, J.W</creatorcontrib><creatorcontrib>Shin, J.S</creatorcontrib><creatorcontrib>Kwon, J.H</creatorcontrib><creatorcontrib>Kim, C.W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, J.J. (Korea Univ., Seoul (Korea R.))</au><au>Yi, H.Y</au><au>Yang, J.W</au><au>Shin, J.S</au><au>Kwon, J.H</au><au>Kim, C.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of streptozotocin-induced diabetic rats and pharmacodynamics of insulin formulations</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2003-11-01</date><risdate>2003</risdate><volume>67</volume><issue>11</issue><spage>2396</spage><epage>2401</epage><pages>2396-2401</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Morphological and functional changes of rat pancreatic islets caused by administration of streptozotocin (STZ) and the bioavailability of insulin formulations administered to STZ-induced diabetic rats with fasting (12 h) or non-fasting were investigated. Islets isolated from normal rats maintained a good three-dimensional structure and the islet yield was 962.5 =+- 86.5 islet equivalent number (IEQ, islets converted to an average diameter of 150 micro m). In the diabetic group (>500 mg/ ml blood glucose), the islet yield was only 44.4 =+- 8.3 IEQ and the islet was severely damaged. The minimum reduction of blood glucose of each formulation, such as insulin solution, microcrystal, and insulin microcrystal capsule, was shown to be 11.3, 11.0, and 16.3 mg/dl, respectively, at 6 h in fasting with diabetic rats. These results indicated that the administration of insulin formulations to the fasting groups increased the severe hypoglycemic effect of insulin action more than in non-fasting diabetic rats. The diabetic rat with fasting has a regulatory disorder in maintaining the blood glucose level. Accordingly, the validity of pharmacological availability as an optimal modeling of insulin formulations is best in non-fasting STZ-induced diabetic rats.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>14646199</pmid><doi>10.1271/bbb.67.2396</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2003-11, Vol.67 (11), p.2396-2401 |
| issn | 0916-8451 1347-6947 |
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| source | J-STAGE Free; MEDLINE; Oxford University Press Journals All Titles (1996-Current); Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals ANTIBIOTICS Biological and medical sciences Blood Glucose - drug effects Blood Glucose - metabolism Cell Separation DIABETES Diabetes Mellitus, Experimental - metabolism Diabetes Mellitus, Experimental - pathology Fundamental and applied biological sciences. Psychology Glucose Tolerance Test Hypoglycemic Agents - pharmacokinetics Hypoglycemic Agents - therapeutic use INSULIN Insulin - pharmacokinetics Insulin - therapeutic use islet Islets of Langerhans - pathology Islets of Langerhans - secretion Kinetics Male MANKIND Pancreas - pathology pharmacodynamics PHARMACOLOGY Rats Rats, Sprague-Dawley streptozotocin |
| title | Characterization of streptozotocin-induced diabetic rats and pharmacodynamics of insulin formulations |
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