Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants
For preliminary screening for and characterization of free-living nitrogen-fixing bacteria from rhizoplane microflora, we used Winogradsky's mineral mixture-based nitrogen-free medium solidified with 0.3% gellan gum. The soft gel medium enabled some reference and wild free-living nitrogen-fixin...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-10, Vol.66 (10), p.2259-2263 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Hashidoko, Y. (Hokkaido Univ., Sapporo (Japan)) Tada, M Osaki, M Tahara, S |
| description | For preliminary screening for and characterization of free-living nitrogen-fixing bacteria from rhizoplane microflora, we used Winogradsky's mineral mixture-based nitrogen-free medium solidified with 0.3% gellan gum. The soft gel medium enabled some reference and wild free-living nitrogen-fixing bacteria to grow in characteristic colonies, including their reaction to oxygen and their motility change. Gellan gum is thus likely to be a better gel matrix than agarose for the investigation of root-associating, free-living nitrogen-fixing bacteria to identify their characteristic behaviors. |
| doi_str_mv | 10.1271/bbb.66.2259 |
| format | Article |
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(Hokkaido Univ., Sapporo (Japan)) ; Tada, M ; Osaki, M ; Tahara, S</creator><creatorcontrib>Hashidoko, Y. (Hokkaido Univ., Sapporo (Japan)) ; Tada, M ; Osaki, M ; Tahara, S</creatorcontrib><description>For preliminary screening for and characterization of free-living nitrogen-fixing bacteria from rhizoplane microflora, we used Winogradsky's mineral mixture-based nitrogen-free medium solidified with 0.3% gellan gum. The soft gel medium enabled some reference and wild free-living nitrogen-fixing bacteria to grow in characteristic colonies, including their reaction to oxygen and their motility change. Gellan gum is thus likely to be a better gel matrix than agarose for the investigation of root-associating, free-living nitrogen-fixing bacteria to identify their characteristic behaviors.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.66.2259</identifier><identifier>PMID: 12450146</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Bacteria - isolation & purification ; Bacteria - metabolism ; Biological and medical sciences ; Caulobacter - chemistry ; Caulobacter - metabolism ; COLLOIDS ; COLONIZING ABILITY ; CULTURE MEDIA ; CULTURE TECHNIQUES ; free-living nitrogen-fixing bacterium ; Fundamental and applied biological sciences. Psychology ; gellan gum ; Gels ; GUMS ; Klebsiella pneumoniae - growth & development ; MICROBIAL FLORA ; Nitrogen Fixation - physiology ; NITROGEN FIXING BACTERIA ; Plant Roots - microbiology ; Plantago - metabolism ; PLANTAGO LANCEOLATA ; Polysaccharides, Bacterial - chemistry ; Proteobacteria - metabolism ; rhizoplane microflora ; RHIZOSPHERE ; SELECTION ; Sepharose ; Zoogloea - chemistry ; Zoogloea - metabolism</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2002-10, Vol.66 (10), p.2259-2263</ispartof><rights>2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2002</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c590t-227e235af5f6db84e0ec00a8937a070bd47defa36455c5d6d919bf0ad39830613</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14993261$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12450146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashidoko, Y. (Hokkaido Univ., Sapporo (Japan))</creatorcontrib><creatorcontrib>Tada, M</creatorcontrib><creatorcontrib>Osaki, M</creatorcontrib><creatorcontrib>Tahara, S</creatorcontrib><title>Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>For preliminary screening for and characterization of free-living nitrogen-fixing bacteria from rhizoplane microflora, we used Winogradsky's mineral mixture-based nitrogen-free medium solidified with 0.3% gellan gum. The soft gel medium enabled some reference and wild free-living nitrogen-fixing bacteria to grow in characteristic colonies, including their reaction to oxygen and their motility change. Gellan gum is thus likely to be a better gel matrix than agarose for the investigation of root-associating, free-living nitrogen-fixing bacteria to identify their characteristic behaviors.</description><subject>Bacteria - isolation & purification</subject><subject>Bacteria - metabolism</subject><subject>Biological and medical sciences</subject><subject>Caulobacter - chemistry</subject><subject>Caulobacter - metabolism</subject><subject>COLLOIDS</subject><subject>COLONIZING ABILITY</subject><subject>CULTURE MEDIA</subject><subject>CULTURE TECHNIQUES</subject><subject>free-living nitrogen-fixing bacterium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gellan gum</subject><subject>Gels</subject><subject>GUMS</subject><subject>Klebsiella pneumoniae - growth & development</subject><subject>MICROBIAL FLORA</subject><subject>Nitrogen Fixation - physiology</subject><subject>NITROGEN FIXING BACTERIA</subject><subject>Plant Roots - microbiology</subject><subject>Plantago - metabolism</subject><subject>PLANTAGO LANCEOLATA</subject><subject>Polysaccharides, Bacterial - chemistry</subject><subject>Proteobacteria - metabolism</subject><subject>rhizoplane microflora</subject><subject>RHIZOSPHERE</subject><subject>SELECTION</subject><subject>Sepharose</subject><subject>Zoogloea - chemistry</subject><subject>Zoogloea - metabolism</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuLFDEUhQtRnHZ05VoJiLPRavNOZSmD44MBBXUdUqmkO0NV0iYpx_Hv-EdNdbcMiOAqyT3fvcnJaZrHCK4RFuhV3_drztcYM3mnWSFCRcslFXebFZSItx1l6KR5kPMVhLXA0P3mBGHKIKJ81fz6HF0BGzuCyQ5-nkCOox-883YA175sF2nUAWyq5GICu2RHP_mg0w3IJlkbfNjslRRjaXXO0XhdavElcFVuR_99IYIvKW5saJ3_sZx7bYpNXgMftrr3SwMoWwvS1v-Mu3qjBdGBZVPyw-ae02O2j47rafP14s2X83ft5ce3789fX7aGSVhajIXFhGnHHB_6jlpoDYS6k0RoKGA_UDFYpwmnjBk28EEi2TuoByI7Ajkip83ZYe4uxW-zzUVNPpu9fxvnrAQWUAqG_wuiroOSkgV89hd4FecUqgmFKJWUdpyTSr04UCbFnJN1apf8VH9YIaiWiFWNWHGulogr_fQ4c-5rZrfsMdMKPD8COhs9uqSD8fmWo1ISvLfLD5wPNb9JX8c0DqromzGmP03k3y94cmh0Oiq9SZX78AlDSCDEncDkN_SlzL8</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Hashidoko, Y. 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(Hokkaido Univ., Sapporo (Japan)) ; Tada, M ; Osaki, M ; Tahara, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c590t-227e235af5f6db84e0ec00a8937a070bd47defa36455c5d6d919bf0ad39830613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Bacteria - isolation & purification</topic><topic>Bacteria - metabolism</topic><topic>Biological and medical sciences</topic><topic>Caulobacter - chemistry</topic><topic>Caulobacter - metabolism</topic><topic>COLLOIDS</topic><topic>COLONIZING ABILITY</topic><topic>CULTURE MEDIA</topic><topic>CULTURE TECHNIQUES</topic><topic>free-living nitrogen-fixing bacterium</topic><topic>Fundamental and applied biological sciences. 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(Hokkaido Univ., Sapporo (Japan))</creatorcontrib><creatorcontrib>Tada, M</creatorcontrib><creatorcontrib>Osaki, M</creatorcontrib><creatorcontrib>Tahara, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashidoko, Y. 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The soft gel medium enabled some reference and wild free-living nitrogen-fixing bacteria to grow in characteristic colonies, including their reaction to oxygen and their motility change. Gellan gum is thus likely to be a better gel matrix than agarose for the investigation of root-associating, free-living nitrogen-fixing bacteria to identify their characteristic behaviors.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>12450146</pmid><doi>10.1271/bbb.66.2259</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Bacteria - isolation & purification Bacteria - metabolism Biological and medical sciences Caulobacter - chemistry Caulobacter - metabolism COLLOIDS COLONIZING ABILITY CULTURE MEDIA CULTURE TECHNIQUES free-living nitrogen-fixing bacterium Fundamental and applied biological sciences. Psychology gellan gum Gels GUMS Klebsiella pneumoniae - growth & development MICROBIAL FLORA Nitrogen Fixation - physiology NITROGEN FIXING BACTERIA Plant Roots - microbiology Plantago - metabolism PLANTAGO LANCEOLATA Polysaccharides, Bacterial - chemistry Proteobacteria - metabolism rhizoplane microflora RHIZOSPHERE SELECTION Sepharose Zoogloea - chemistry Zoogloea - metabolism |
| title | Soft gel medium solidified with gellan gum for preliminary screening for root-associating, free-living nitrogen-fixing bacteria inhabiting the rhizoplane of plants |
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