Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis
The three species of the group 1 bacilli, Bacillus anthracis, B. cereus, and B. thuringiensis, are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes. B. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while B. cereus and B....
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| Veröffentlicht in: | Applied and Environmental Microbiology 2003-05, Vol.69 (5), p.2755-2764 |
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| creator | Radnedge, L Agron, P.G Hill, K.K Jackson, P.J Ticknor, L.O Keim, P Andersen, G.L |
| description | The three species of the group 1 bacilli, Bacillus anthracis, B. cereus, and B. thuringiensis, are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes. B. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while B. cereus and B. thuringiensis are genetically more diverse. An amplified fragment length polymorphism analysis described here demonstrates genetic diversity among a collection of non-anthrax-causing Bacillus species, some of which show significant similarity to B. anthracis. Suppression subtractive hybridization was then used to characterize the genomic differences that distinguish three of the non-anthrax-causing bacilli from B. anthracis Ames. Ninety-three DNA sequences that were present in B. anthracis but absent from the non-anthrax-causing Bacillus genomes were isolated. Furthermore, 28 of these sequences were not found in a collection of 10 non-anthrax-causing Bacillus species but were present in all members of a representative collection of B. anthracis strains. These sequences map to distinct loci on the B. anthracis genome and can be assayed simultaneously in multiplex PCR assays for rapid and highly specific DNA-based detection of B. anthracis. |
| doi_str_mv | 10.1128/AEM.69.5.2755-2764.2003 |
| format | Article |
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(LBNL), Berkeley, CA (United States)</creatorcontrib><description>The three species of the group 1 bacilli, Bacillus anthracis, B. cereus, and B. thuringiensis, are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes. B. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while B. cereus and B. thuringiensis are genetically more diverse. An amplified fragment length polymorphism analysis described here demonstrates genetic diversity among a collection of non-anthrax-causing Bacillus species, some of which show significant similarity to B. anthracis. Suppression subtractive hybridization was then used to characterize the genomic differences that distinguish three of the non-anthrax-causing bacilli from B. anthracis Ames. Ninety-three DNA sequences that were present in B. anthracis but absent from the non-anthrax-causing Bacillus genomes were isolated. Furthermore, 28 of these sequences were not found in a collection of 10 non-anthrax-causing Bacillus species but were present in all members of a representative collection of B. anthracis strains. These sequences map to distinct loci on the B. anthracis genome and can be assayed simultaneously in multiplex PCR assays for rapid and highly specific DNA-based detection of B. anthracis.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.69.5.2755-2764.2003</identifier><identifier>PMID: 12732546</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>amplified fragment length polymorphism ; animal pathogenic bacteria ; BACILLUS ; Bacillus - classification ; Bacillus - genetics ; Bacillus anthracis ; Bacillus anthracis - genetics ; Bacillus anthracis - pathogenicity ; BACILLUS CEREUS ; Bacillus cereus - genetics ; Bacillus thuringiensis ; Bacillus thuringiensis - genetics ; Bacteria ; Base Sequence ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; DISEASES ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; entomopathogenic bacteria ; Fundamental and applied biological sciences. Psychology ; Genetic diversity ; Genetic Variation ; GENETICS ; genome ; Genome, Bacterial ; genomics ; Genotype & phenotype ; loci ; Microbiology ; nucleic acid hybridization ; Nucleic Acid Hybridization - methods ; nucleotide sequences ; Phenotype ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Public Health Microbiology ; Soil microorganisms ; SPECIES DIVERSITY ; Species Specificity ; suppression subtractive hybridization</subject><ispartof>Applied and Environmental Microbiology, 2003-05, Vol.69 (5), p.2755-2764</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright American Society for Microbiology May 2003</rights><rights>Copyright © 2003, American Society for Microbiology 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c684t-9ce27ef114b69c61d4ebc766984f1526ef1baa5bfe0024d5fbfce07281bdff8c3</citedby><cites>FETCH-LOGICAL-c684t-9ce27ef114b69c61d4ebc766984f1526ef1baa5bfe0024d5fbfce07281bdff8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC154536/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC154536/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14788000$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12732546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/813505$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Radnedge, L</creatorcontrib><creatorcontrib>Agron, P.G</creatorcontrib><creatorcontrib>Hill, K.K</creatorcontrib><creatorcontrib>Jackson, P.J</creatorcontrib><creatorcontrib>Ticknor, L.O</creatorcontrib><creatorcontrib>Keim, P</creatorcontrib><creatorcontrib>Andersen, G.L</creatorcontrib><creatorcontrib>Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)</creatorcontrib><title>Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The three species of the group 1 bacilli, Bacillus anthracis, B. cereus, and B. thuringiensis, are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes. B. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while B. cereus and B. thuringiensis are genetically more diverse. An amplified fragment length polymorphism analysis described here demonstrates genetic diversity among a collection of non-anthrax-causing Bacillus species, some of which show significant similarity to B. anthracis. Suppression subtractive hybridization was then used to characterize the genomic differences that distinguish three of the non-anthrax-causing bacilli from B. anthracis Ames. Ninety-three DNA sequences that were present in B. anthracis but absent from the non-anthrax-causing Bacillus genomes were isolated. Furthermore, 28 of these sequences were not found in a collection of 10 non-anthrax-causing Bacillus species but were present in all members of a representative collection of B. anthracis strains. These sequences map to distinct loci on the B. anthracis genome and can be assayed simultaneously in multiplex PCR assays for rapid and highly specific DNA-based detection of B. anthracis.</description><subject>amplified fragment length polymorphism</subject><subject>animal pathogenic bacteria</subject><subject>BACILLUS</subject><subject>Bacillus - classification</subject><subject>Bacillus - genetics</subject><subject>Bacillus anthracis</subject><subject>Bacillus anthracis - genetics</subject><subject>Bacillus anthracis - pathogenicity</subject><subject>BACILLUS CEREUS</subject><subject>Bacillus cereus - genetics</subject><subject>Bacillus thuringiensis</subject><subject>Bacillus thuringiensis - genetics</subject><subject>Bacteria</subject><subject>Base Sequence</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>DISEASES</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>entomopathogenic bacteria</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>GENETICS</subject><subject>genome</subject><subject>Genome, Bacterial</subject><subject>genomics</subject><subject>Genotype & phenotype</subject><subject>loci</subject><subject>Microbiology</subject><subject>nucleic acid hybridization</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>nucleotide sequences</subject><subject>Phenotype</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><subject>Public Health Microbiology</subject><subject>Soil microorganisms</subject><subject>SPECIES DIVERSITY</subject><subject>Species Specificity</subject><subject>suppression subtractive hybridization</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9v1DAQxSMEokvhK9CABLcs_u_40EOpSkEq4gA9W44z3rhK7GInIL49XnZFgAsnyzO_NyM_v6o6w2iLMWnfXFx93Aq15VsiOW-IFGxLEKIPqg1Gqm04peJhtUFIqYYQhk6qJznfIYQYEu3j6gQTSQlnYlO5awhxgrr3zkGCYCHX82DmUsizD7vF56F-a6wfxyXXJsxDKpdcuxSntW6L9Fe7X2vzsKQywEPIPj-tHjkzZnh2PE-r23dXXy7fNzefrj9cXtw0VrRsbpQFIsFhzDqhrMA9g85KIVTLHOZElFZnDO8cIERYz13nLCBJWtz1zrWWnlbnh7n3SzdBbyHMyYz6PvnJpB86Gq__7gQ_6F38pjFnnIqif3HQx_J6na2fwQ42hgB21i2mHPHCvD7uSPHrAnnWk88WxtEEiEvWxVqhJJf_BXErGUJSFfDlP-BdXFIoRmmCuJJESlIgeYBsijkncL9fhZHeh0KXUGihNNf7UOh9KPQ-FEX5_E9TVt0xBQV4dQRMtmZ0yYTyxyvHZNuW8KzuDH43fPcJtMmTNjCtawtzdmCcidrsUplz-5kgTEsYGcWU0Z9pINbp</recordid><startdate>20030501</startdate><enddate>20030501</enddate><creator>Radnedge, L</creator><creator>Agron, P.G</creator><creator>Hill, K.K</creator><creator>Jackson, P.J</creator><creator>Ticknor, L.O</creator><creator>Keim, P</creator><creator>Andersen, G.L</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>20030501</creationdate><title>Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis</title><author>Radnedge, L ; Agron, P.G ; Hill, K.K ; Jackson, P.J ; Ticknor, L.O ; Keim, P ; Andersen, G.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c684t-9ce27ef114b69c61d4ebc766984f1526ef1baa5bfe0024d5fbfce07281bdff8c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>amplified fragment length polymorphism</topic><topic>animal pathogenic bacteria</topic><topic>BACILLUS</topic><topic>Bacillus - classification</topic><topic>Bacillus - genetics</topic><topic>Bacillus anthracis</topic><topic>Bacillus anthracis - genetics</topic><topic>Bacillus anthracis - pathogenicity</topic><topic>BACILLUS CEREUS</topic><topic>Bacillus cereus - genetics</topic><topic>Bacillus thuringiensis</topic><topic>Bacillus thuringiensis - genetics</topic><topic>Bacteria</topic><topic>Base Sequence</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>DISEASES</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>entomopathogenic bacteria</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic diversity</topic><topic>Genetic Variation</topic><topic>GENETICS</topic><topic>genome</topic><topic>Genome, Bacterial</topic><topic>genomics</topic><topic>Genotype & phenotype</topic><topic>loci</topic><topic>Microbiology</topic><topic>nucleic acid hybridization</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>nucleotide sequences</topic><topic>Phenotype</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><topic>Public Health Microbiology</topic><topic>Soil microorganisms</topic><topic>SPECIES DIVERSITY</topic><topic>Species Specificity</topic><topic>suppression subtractive hybridization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radnedge, L</creatorcontrib><creatorcontrib>Agron, P.G</creatorcontrib><creatorcontrib>Hill, K.K</creatorcontrib><creatorcontrib>Jackson, P.J</creatorcontrib><creatorcontrib>Ticknor, L.O</creatorcontrib><creatorcontrib>Keim, P</creatorcontrib><creatorcontrib>Andersen, G.L</creatorcontrib><creatorcontrib>Lawrence Berkeley National Lab. 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(LBNL), Berkeley, CA (United States)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2003-05-01</date><risdate>2003</risdate><volume>69</volume><issue>5</issue><spage>2755</spage><epage>2764</epage><pages>2755-2764</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>The three species of the group 1 bacilli, Bacillus anthracis, B. cereus, and B. thuringiensis, are genetically very closely related. All inhabit soil habitats but exhibit different phenotypes. B. anthracis is the causative agent of anthrax and is phylogenetically monomorphic, while B. cereus and B. thuringiensis are genetically more diverse. An amplified fragment length polymorphism analysis described here demonstrates genetic diversity among a collection of non-anthrax-causing Bacillus species, some of which show significant similarity to B. anthracis. Suppression subtractive hybridization was then used to characterize the genomic differences that distinguish three of the non-anthrax-causing bacilli from B. anthracis Ames. Ninety-three DNA sequences that were present in B. anthracis but absent from the non-anthrax-causing Bacillus genomes were isolated. Furthermore, 28 of these sequences were not found in a collection of 10 non-anthrax-causing Bacillus species but were present in all members of a representative collection of B. anthracis strains. These sequences map to distinct loci on the B. anthracis genome and can be assayed simultaneously in multiplex PCR assays for rapid and highly specific DNA-based detection of B. anthracis.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>12732546</pmid><doi>10.1128/AEM.69.5.2755-2764.2003</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | amplified fragment length polymorphism animal pathogenic bacteria BACILLUS Bacillus - classification Bacillus - genetics Bacillus anthracis Bacillus anthracis - genetics Bacillus anthracis - pathogenicity BACILLUS CEREUS Bacillus cereus - genetics Bacillus thuringiensis Bacillus thuringiensis - genetics Bacteria Base Sequence BASIC BIOLOGICAL SCIENCES Biological and medical sciences DISEASES DNA, Bacterial - genetics DNA, Bacterial - isolation & purification entomopathogenic bacteria Fundamental and applied biological sciences. Psychology Genetic diversity Genetic Variation GENETICS genome Genome, Bacterial genomics Genotype & phenotype loci Microbiology nucleic acid hybridization Nucleic Acid Hybridization - methods nucleotide sequences Phenotype Polymerase Chain Reaction Polymorphism, Genetic Public Health Microbiology Soil microorganisms SPECIES DIVERSITY Species Specificity suppression subtractive hybridization |
| title | Genome differences that distinguish Bacillus anthracis from Bacillus cereus and Bacillus thuringiensis |
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