Effects of degree of hydrolysis and pH on the solubility of heme-iron enriched peptide in hemoglobin hydrolysate
Hemoglobin was hydrolyzed with Esperase and Flavourzyme as the endopeptidase and exopeptidase, respectively. The solubility of the heme-iron enriched peptide fraction decreased as the degree of hydrolysis of the hydrolysate increased. When the pH of a hydrolysate was adjusted to 5.0 after simultaneo...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-02, Vol.67 (2), p.365-367 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | In, M.J. (Chungwoon Univ., Hongsung (Korea R.)) Kim, D.C Chae, H.J Oh, N.S |
| description | Hemoglobin was hydrolyzed with Esperase and Flavourzyme as the endopeptidase and exopeptidase, respectively. The solubility of the heme-iron enriched peptide fraction decreased as the degree of hydrolysis of the hydrolysate increased. When the pH of a hydrolysate was adjusted to 5.0 after simultaneous hydrolysis with the two enzymes, the solubility of heme-iron enriched peptide was nearly zero, and 98% of the heme-iron enriched peptide fraction was recovered as a precipitate. These results indicated that an effective separation method for the production of heme-iron enriched peptide could be established by pH adjustment of the hemoglobin hydrolysate with high degree of hydrolysis. |
| doi_str_mv | 10.1271/bbb.67.365 |
| format | Article |
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(Chungwoon Univ., Hongsung (Korea R.)) ; Kim, D.C ; Chae, H.J ; Oh, N.S</creator><creatorcontrib>In, M.J. (Chungwoon Univ., Hongsung (Korea R.)) ; Kim, D.C ; Chae, H.J ; Oh, N.S</creatorcontrib><description>Hemoglobin was hydrolyzed with Esperase and Flavourzyme as the endopeptidase and exopeptidase, respectively. The solubility of the heme-iron enriched peptide fraction decreased as the degree of hydrolysis of the hydrolysate increased. When the pH of a hydrolysate was adjusted to 5.0 after simultaneous hydrolysis with the two enzymes, the solubility of heme-iron enriched peptide was nearly zero, and 98% of the heme-iron enriched peptide fraction was recovered as a precipitate. These results indicated that an effective separation method for the production of heme-iron enriched peptide could be established by pH adjustment of the hemoglobin hydrolysate with high degree of hydrolysis.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.67.365</identifier><identifier>PMID: 12728999</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>Biological and medical sciences ; CATTLE ; degree of hydrolysis ; Endopeptidases - chemistry ; Fractional Precipitation ; Fundamental and applied biological sciences. Psychology ; HAEMOGLOBIN ; Heme - chemistry ; heme-iron enriched peptide ; Hemeproteins - chemistry ; hemoglobin ; Hemoglobins - chemistry ; Hydrogen-Ion Concentration ; HYDROLYSIS ; HYDROLYZED PROTEINS ; IRON ; PEPTIDES ; pH adjustment ; Serine Endopeptidases - chemistry ; SOLUBILITY ; Time Factors</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2003-02, Vol.67 (2), p.365-367</ispartof><rights>2003 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2003</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c661t-ec457af3afa085282657c33dc966c3e7564141321b6029016e7a97be0b8d9ed03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14738484$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12728999$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>In, M.J. (Chungwoon Univ., Hongsung (Korea R.))</creatorcontrib><creatorcontrib>Kim, D.C</creatorcontrib><creatorcontrib>Chae, H.J</creatorcontrib><creatorcontrib>Oh, N.S</creatorcontrib><title>Effects of degree of hydrolysis and pH on the solubility of heme-iron enriched peptide in hemoglobin hydrolysate</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Hemoglobin was hydrolyzed with Esperase and Flavourzyme as the endopeptidase and exopeptidase, respectively. The solubility of the heme-iron enriched peptide fraction decreased as the degree of hydrolysis of the hydrolysate increased. When the pH of a hydrolysate was adjusted to 5.0 after simultaneous hydrolysis with the two enzymes, the solubility of heme-iron enriched peptide was nearly zero, and 98% of the heme-iron enriched peptide fraction was recovered as a precipitate. These results indicated that an effective separation method for the production of heme-iron enriched peptide could be established by pH adjustment of the hemoglobin hydrolysate with high degree of hydrolysis.</description><subject>Biological and medical sciences</subject><subject>CATTLE</subject><subject>degree of hydrolysis</subject><subject>Endopeptidases - chemistry</subject><subject>Fractional Precipitation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HAEMOGLOBIN</subject><subject>Heme - chemistry</subject><subject>heme-iron enriched peptide</subject><subject>Hemeproteins - chemistry</subject><subject>hemoglobin</subject><subject>Hemoglobins - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>HYDROLYSIS</subject><subject>HYDROLYZED PROTEINS</subject><subject>IRON</subject><subject>PEPTIDES</subject><subject>pH adjustment</subject><subject>Serine Endopeptidases - chemistry</subject><subject>SOLUBILITY</subject><subject>Time Factors</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0c2L1TAQAPAgivtcvXhXCqIHoc-k-WqOy7K6yoIe9BzSdPJelrR5Ji3S_97UV1kQIZAh85thyCD0kuA9aST50HXdXsg9FfwR2hHKZC0Uk4_RDisi6pZxcoGe5XyPcXng5Cm6KGVNq5TaodONc2CnXEVX9XBIAGt0XPoUw5J9rszYV6fbKo7VdIQqxzB3Pvhp-cNggNqnkoMxeXuEQuE0-R4qP67ZeAixW8Otn5ngOXriTMjwYrsv0Y-PN9-vb-u7r58-X1_d1VYIMtVgGZfGUeMMbnnTNoJLS2lvlRCWguSCEUZoQzqBG4WJAGmU7AB3ba-gx_QSvTv3PaX4c4Y86cFnCyGYEeKctaSNwISv8M0_8D7OaSyzacKYYpwSzIt6f1Y2xZwTOH1KfjBp0QTrdQu6bEELqcsWCn69tZy7AfoHun17AW83YLI1wSUzWp8fHJO0ZS0rjp-dH11Mg_kVU-j1ZJYQ098i-t8BXp3rnInaHFJhX741GNNyBG7pb0Xsqkk</recordid><startdate>20030201</startdate><enddate>20030201</enddate><creator>In, M.J. (Chungwoon Univ., Hongsung (Korea R.))</creator><creator>Kim, D.C</creator><creator>Chae, H.J</creator><creator>Oh, N.S</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030201</creationdate><title>Effects of degree of hydrolysis and pH on the solubility of heme-iron enriched peptide in hemoglobin hydrolysate</title><author>In, M.J. (Chungwoon Univ., Hongsung (Korea R.)) ; Kim, D.C ; Chae, H.J ; Oh, N.S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c661t-ec457af3afa085282657c33dc966c3e7564141321b6029016e7a97be0b8d9ed03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>CATTLE</topic><topic>degree of hydrolysis</topic><topic>Endopeptidases - chemistry</topic><topic>Fractional Precipitation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HAEMOGLOBIN</topic><topic>Heme - chemistry</topic><topic>heme-iron enriched peptide</topic><topic>Hemeproteins - chemistry</topic><topic>hemoglobin</topic><topic>Hemoglobins - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>HYDROLYSIS</topic><topic>HYDROLYZED PROTEINS</topic><topic>IRON</topic><topic>PEPTIDES</topic><topic>pH adjustment</topic><topic>Serine Endopeptidases - chemistry</topic><topic>SOLUBILITY</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>In, M.J. (Chungwoon Univ., Hongsung (Korea R.))</creatorcontrib><creatorcontrib>Kim, D.C</creatorcontrib><creatorcontrib>Chae, H.J</creatorcontrib><creatorcontrib>Oh, N.S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>In, M.J. 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When the pH of a hydrolysate was adjusted to 5.0 after simultaneous hydrolysis with the two enzymes, the solubility of heme-iron enriched peptide was nearly zero, and 98% of the heme-iron enriched peptide fraction was recovered as a precipitate. These results indicated that an effective separation method for the production of heme-iron enriched peptide could be established by pH adjustment of the hemoglobin hydrolysate with high degree of hydrolysis.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>12728999</pmid><doi>10.1271/bbb.67.365</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0916-8451 |
| ispartof | Bioscience, biotechnology, and biochemistry, 2003-02, Vol.67 (2), p.365-367 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_14738484 |
| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Biological and medical sciences CATTLE degree of hydrolysis Endopeptidases - chemistry Fractional Precipitation Fundamental and applied biological sciences. Psychology HAEMOGLOBIN Heme - chemistry heme-iron enriched peptide Hemeproteins - chemistry hemoglobin Hemoglobins - chemistry Hydrogen-Ion Concentration HYDROLYSIS HYDROLYZED PROTEINS IRON PEPTIDES pH adjustment Serine Endopeptidases - chemistry SOLUBILITY Time Factors |
| title | Effects of degree of hydrolysis and pH on the solubility of heme-iron enriched peptide in hemoglobin hydrolysate |
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