A rapid HPLC method for determination of major phenolic acids in plant material
The present work describes a simplified, and rapid HPLC method for measurement of phenolic acids in plant material. This procedure allows baseline resolution of major phenolic acids commonly found in plants ( p-hydroxybenzoic, caffeic, syringic, o-coumaric, m-coumaric, p-coumaric, gentisic, ferulic,...
Gespeichert in:
| Veröffentlicht in: | Polish journal of food and nutrition sciences 2003, Vol.12 (1), p.53-57 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 57 |
|---|---|
| container_issue | 1 |
| container_start_page | 53 |
| container_title | Polish journal of food and nutrition sciences |
| container_volume | 12 |
| creator | Olkowski, A.A Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science) Peiquiang, Yu McKinnon, J.J Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science) |
| description | The present work describes a simplified, and rapid HPLC method for measurement of phenolic acids in plant material. This procedure allows baseline resolution of major phenolic acids commonly found in plants ( p-hydroxybenzoic, caffeic, syringic, o-coumaric, m-coumaric, p-coumaric, gentisic, ferulic, sinapic, salicylic), it is specific, sensitive, and ensures good reproducibility. The precision and reproducibility obtained using the present approach gave results comparable or better than the more complex, more laborious, and time consuming procedures. The time of HPLC separation of the phenolic acids in the presented procedure was significantly improved by the use of polymer based reverse phase column, and careful attention to the pH of the mobile phase, which allowed turnaround times of approximately 15 min and significantly lower solvent use. The present method allows the possibility for processing of a large number of samples rapidly, efficiently, and at a low cost |
| format | Article |
| fullrecord | <record><control><sourceid>fao_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_14687300</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>PL2004000751</sourcerecordid><originalsourceid>FETCH-LOGICAL-f520-3d2e7392b810581c24bdb749121423f47f63317987d438f0f8edf5fd35321d073</originalsourceid><addsrcrecordid>eNotkM1qwzAQhEVpoSHJIxR06dGw2pUt5RhC2xQMySF3s7GkRsF_yL707StI5zIw8zGHeRIrBEtFBWCexUohQQGE-Cq283yHrFJVhHYlTnuZeIpOHs_1QfZ-uY1OhjFJ5xef-jjwEsdBjkH2fM_xdPPD2MVWchvdLOMgp46HJbcZj9xtxEvgbvbbf1-Ly-fH5XAs6tPX92FfF6FEKMihN7TDq1VQWtWivrqr0TuFSiMFbUJFpMzOGqfJBgjWu1AGRyWhcmBoLd4fsxPPLXch8dDGuZlS7Dn9NkpX1hBA5t4eXOCx4Z-UmXONADpfYEpFf5jnU20</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>A rapid HPLC method for determination of major phenolic acids in plant material</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Olkowski, A.A ; Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science) ; Peiquiang, Yu ; McKinnon, J.J ; Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science)</creator><creatorcontrib>Olkowski, A.A ; Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science) ; Peiquiang, Yu ; McKinnon, J.J ; Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science)</creatorcontrib><description>The present work describes a simplified, and rapid HPLC method for measurement of phenolic acids in plant material. This procedure allows baseline resolution of major phenolic acids commonly found in plants ( p-hydroxybenzoic, caffeic, syringic, o-coumaric, m-coumaric, p-coumaric, gentisic, ferulic, sinapic, salicylic), it is specific, sensitive, and ensures good reproducibility. The precision and reproducibility obtained using the present approach gave results comparable or better than the more complex, more laborious, and time consuming procedures. The time of HPLC separation of the phenolic acids in the presented procedure was significantly improved by the use of polymer based reverse phase column, and careful attention to the pH of the mobile phase, which allowed turnaround times of approximately 15 min and significantly lower solvent use. The present method allows the possibility for processing of a large number of samples rapidly, efficiently, and at a low cost</description><identifier>ISSN: 1230-0322</identifier><identifier>EISSN: 2083-6007</identifier><language>eng</language><publisher>Olsztyn: Polish Academy of Sciences</publisher><subject>ACIDE PHÉNOLIQUE ; Biological and medical sciences ; CONTENIDO FENÓLICO ; COQUE DE GRAIN ; CÁSCARAS ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; HARINA DE COLZA ; HPLC ; HUSKS ; Methods of analysis, processing and quality control, regulation, standards ; PHENOLIC ACIDS ; PHENOLIC CONTENT ; RAPESEED MEAL ; TENEUR EN PHÉNOLS ; TOURTEAU DE COLZA ; ÁCIDOS FENOLITOS</subject><ispartof>Polish journal of food and nutrition sciences, 2003, Vol.12 (1), p.53-57</ispartof><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4009</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14687300$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Olkowski, A.A</creatorcontrib><creatorcontrib>Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science)</creatorcontrib><creatorcontrib>Peiquiang, Yu</creatorcontrib><creatorcontrib>McKinnon, J.J</creatorcontrib><creatorcontrib>Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science)</creatorcontrib><title>A rapid HPLC method for determination of major phenolic acids in plant material</title><title>Polish journal of food and nutrition sciences</title><description>The present work describes a simplified, and rapid HPLC method for measurement of phenolic acids in plant material. This procedure allows baseline resolution of major phenolic acids commonly found in plants ( p-hydroxybenzoic, caffeic, syringic, o-coumaric, m-coumaric, p-coumaric, gentisic, ferulic, sinapic, salicylic), it is specific, sensitive, and ensures good reproducibility. The precision and reproducibility obtained using the present approach gave results comparable or better than the more complex, more laborious, and time consuming procedures. The time of HPLC separation of the phenolic acids in the presented procedure was significantly improved by the use of polymer based reverse phase column, and careful attention to the pH of the mobile phase, which allowed turnaround times of approximately 15 min and significantly lower solvent use. The present method allows the possibility for processing of a large number of samples rapidly, efficiently, and at a low cost</description><subject>ACIDE PHÉNOLIQUE</subject><subject>Biological and medical sciences</subject><subject>CONTENIDO FENÓLICO</subject><subject>COQUE DE GRAIN</subject><subject>CÁSCARAS</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>HARINA DE COLZA</subject><subject>HPLC</subject><subject>HUSKS</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>PHENOLIC ACIDS</subject><subject>PHENOLIC CONTENT</subject><subject>RAPESEED MEAL</subject><subject>TENEUR EN PHÉNOLS</subject><subject>TOURTEAU DE COLZA</subject><subject>ÁCIDOS FENOLITOS</subject><issn>1230-0322</issn><issn>2083-6007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNotkM1qwzAQhEVpoSHJIxR06dGw2pUt5RhC2xQMySF3s7GkRsF_yL707StI5zIw8zGHeRIrBEtFBWCexUohQQGE-Cq283yHrFJVhHYlTnuZeIpOHs_1QfZ-uY1OhjFJ5xef-jjwEsdBjkH2fM_xdPPD2MVWchvdLOMgp46HJbcZj9xtxEvgbvbbf1-Ly-fH5XAs6tPX92FfF6FEKMihN7TDq1VQWtWivrqr0TuFSiMFbUJFpMzOGqfJBgjWu1AGRyWhcmBoLd4fsxPPLXch8dDGuZlS7Dn9NkpX1hBA5t4eXOCx4Z-UmXONADpfYEpFf5jnU20</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Olkowski, A.A</creator><creator>Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science)</creator><creator>Peiquiang, Yu</creator><creator>McKinnon, J.J</creator><creator>Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science)</creator><general>Polish Academy of Sciences</general><general>Institute of Animal Reproduction</general><scope>FBQ</scope><scope>IQODW</scope></search><sort><creationdate>2003</creationdate><title>A rapid HPLC method for determination of major phenolic acids in plant material</title><author>Olkowski, A.A ; Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science) ; Peiquiang, Yu ; McKinnon, J.J ; Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f520-3d2e7392b810581c24bdb749121423f47f63317987d438f0f8edf5fd35321d073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>ACIDE PHÉNOLIQUE</topic><topic>Biological and medical sciences</topic><topic>CONTENIDO FENÓLICO</topic><topic>COQUE DE GRAIN</topic><topic>CÁSCARAS</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>HARINA DE COLZA</topic><topic>HPLC</topic><topic>HUSKS</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>PHENOLIC ACIDS</topic><topic>PHENOLIC CONTENT</topic><topic>RAPESEED MEAL</topic><topic>TENEUR EN PHÉNOLS</topic><topic>TOURTEAU DE COLZA</topic><topic>ÁCIDOS FENOLITOS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Olkowski, A.A</creatorcontrib><creatorcontrib>Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science)</creatorcontrib><creatorcontrib>Peiquiang, Yu</creatorcontrib><creatorcontrib>McKinnon, J.J</creatorcontrib><creatorcontrib>Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Polish journal of food and nutrition sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Olkowski, A.A</au><au>Amarowicz, R. (Polish Academy of Sciences, Olsztyn (Poland). Inst. of Animal Reproduction and Food Research, Div. of Food Science)</au><au>Peiquiang, Yu</au><au>McKinnon, J.J</au><au>Maenz D.D (Saskatchewan University (Canada). Dept. of Animal and Poultry Science)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A rapid HPLC method for determination of major phenolic acids in plant material</atitle><jtitle>Polish journal of food and nutrition sciences</jtitle><date>2003</date><risdate>2003</risdate><volume>12</volume><issue>1</issue><spage>53</spage><epage>57</epage><pages>53-57</pages><issn>1230-0322</issn><eissn>2083-6007</eissn><abstract>The present work describes a simplified, and rapid HPLC method for measurement of phenolic acids in plant material. This procedure allows baseline resolution of major phenolic acids commonly found in plants ( p-hydroxybenzoic, caffeic, syringic, o-coumaric, m-coumaric, p-coumaric, gentisic, ferulic, sinapic, salicylic), it is specific, sensitive, and ensures good reproducibility. The precision and reproducibility obtained using the present approach gave results comparable or better than the more complex, more laborious, and time consuming procedures. The time of HPLC separation of the phenolic acids in the presented procedure was significantly improved by the use of polymer based reverse phase column, and careful attention to the pH of the mobile phase, which allowed turnaround times of approximately 15 min and significantly lower solvent use. The present method allows the possibility for processing of a large number of samples rapidly, efficiently, and at a low cost</abstract><cop>Olsztyn</cop><pub>Polish Academy of Sciences</pub><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1230-0322 |
| ispartof | Polish journal of food and nutrition sciences, 2003, Vol.12 (1), p.53-57 |
| issn | 1230-0322 2083-6007 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_14687300 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | ACIDE PHÉNOLIQUE Biological and medical sciences CONTENIDO FENÓLICO COQUE DE GRAIN CÁSCARAS Food industries Fundamental and applied biological sciences. Psychology General aspects HARINA DE COLZA HPLC HUSKS Methods of analysis, processing and quality control, regulation, standards PHENOLIC ACIDS PHENOLIC CONTENT RAPESEED MEAL TENEUR EN PHÉNOLS TOURTEAU DE COLZA ÁCIDOS FENOLITOS |
| title | A rapid HPLC method for determination of major phenolic acids in plant material |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T12%3A29%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20rapid%20HPLC%20method%20for%20determination%20of%20major%20phenolic%20acids%20in%20plant%20material&rft.jtitle=Polish%20journal%20of%20food%20and%20nutrition%20sciences&rft.au=Olkowski,%20A.A&rft.date=2003&rft.volume=12&rft.issue=1&rft.spage=53&rft.epage=57&rft.pages=53-57&rft.issn=1230-0322&rft.eissn=2083-6007&rft_id=info:doi/&rft_dat=%3Cfao_pasca%3EPL2004000751%3C/fao_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |