Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability
Fluorescein is a marker-dye customary applied to the evaluation of tight-junctional permeability of epithelial cell monolayers. However, the true mechanism for the permeation has not been elucidated. Transepithelial transport of fluorescein in Caco-2 cell monolayers was therefore examined. Fluoresce...
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| Veröffentlicht in: | Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-11, Vol.66 (11), p.2449-2457 |
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| container_title | Bioscience, biotechnology, and biochemistry |
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| creator | Konishi, Y. (Kirin Brewery Co. Ltd., Takasaki, Gunma (Japan). Research Labs.) Hagiwara, K Shimizu, M |
| description | Fluorescein is a marker-dye customary applied to the evaluation of tight-junctional permeability of epithelial cell monolayers. However, the true mechanism for the permeation has not been elucidated. Transepithelial transport of fluorescein in Caco-2 cell monolayers was therefore examined. Fluorescein transport was dependent on pH, and in a vectorical way in the apical-basolateral direction, but it was independent of the tight-junctional permeability of monolayers of these human intestinal cells. The permeation of fluorescein was concentration-dependent and saturable; the Michaelis constant was 7.7 mM and the maximum velocity was 40.3 nmol min
−1
(mg protein)
−1
. Benzoic acid competitively inhibited fluorescein transport, suggesting that fluorescein is transported by a monocarboxylic acid transporter (MCT). Antioxidative polyphenolic compounds such as ferulic acid from dietary sources, competitively inhibited the permeation of fluorescein. These compounds probably share a transport carrier with fluorescein. Measurement of the effects of phenolic acids on fluorescein transport across Caco-2 monolayers would be a useful way to evaluate the intestinal absorption or bioavailability of dietary phenolic acids. |
| doi_str_mv | 10.1271/bbb.66.2449 |
| format | Article |
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−1
(mg protein)
−1
. Benzoic acid competitively inhibited fluorescein transport, suggesting that fluorescein is transported by a monocarboxylic acid transporter (MCT). Antioxidative polyphenolic compounds such as ferulic acid from dietary sources, competitively inhibited the permeation of fluorescein. These compounds probably share a transport carrier with fluorescein. Measurement of the effects of phenolic acids on fluorescein transport across Caco-2 monolayers would be a useful way to evaluate the intestinal absorption or bioavailability of dietary phenolic acids.</description><identifier>ISSN: 0916-8451</identifier><identifier>EISSN: 1347-6947</identifier><identifier>DOI: 10.1271/bbb.66.2449</identifier><identifier>PMID: 12506986</identifier><language>eng</language><publisher>Tokyo: Japan Society for Bioscience, Biotechnology, and Agrochemistry</publisher><subject>availability ; Benzoic Acid - pharmacology ; BIOAVAILABILITY ; Biological and medical sciences ; Biological Availability ; Caco-2 Cells ; Cell physiology ; CELLS ; Coumaric Acids - pharmacology ; Drug Interactions ; Electric Impedance ; Epithelial Cells - metabolism ; EPITHELIUM ; ferulic acid ; fluorescein ; Fluorescein - pharmacokinetics ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydrogen-Ion Concentration ; Hydroxybenzoates - pharmacokinetics ; Intestinal Absorption - drug effects ; Intestinal Mucosa - cytology ; Intestinal Mucosa - metabolism ; INTESTINES ; Isoquinolines - metabolism ; Membrane and intracellular transports ; Molecular and cellular biology ; monocarboxylic acid transporter ; Monocarboxylic Acid Transporters - metabolism ; PERMEABILITY ; PHENOLIC ACIDS ; Tight Junctions - drug effects ; Tight Junctions - physiology</subject><ispartof>Bioscience, biotechnology, and biochemistry, 2002-11, Vol.66 (11), p.2449-2457</ispartof><rights>2002 by Japan Society for Bioscience, Biotechnology, and Agrochemistry 2002</rights><rights>2003 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-1a5014dcd29599f1eb73d394f9ebbcd637afba509f2dfc09e776c8336650caaf3</citedby><cites>FETCH-LOGICAL-c412t-1a5014dcd29599f1eb73d394f9ebbcd637afba509f2dfc09e776c8336650caaf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14409130$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12506986$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Konishi, Y. (Kirin Brewery Co. Ltd., Takasaki, Gunma (Japan). Research Labs.)</creatorcontrib><creatorcontrib>Hagiwara, K</creatorcontrib><creatorcontrib>Shimizu, M</creatorcontrib><title>Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability</title><title>Bioscience, biotechnology, and biochemistry</title><addtitle>Biosci Biotechnol Biochem</addtitle><description>Fluorescein is a marker-dye customary applied to the evaluation of tight-junctional permeability of epithelial cell monolayers. However, the true mechanism for the permeation has not been elucidated. Transepithelial transport of fluorescein in Caco-2 cell monolayers was therefore examined. Fluorescein transport was dependent on pH, and in a vectorical way in the apical-basolateral direction, but it was independent of the tight-junctional permeability of monolayers of these human intestinal cells. The permeation of fluorescein was concentration-dependent and saturable; the Michaelis constant was 7.7 mM and the maximum velocity was 40.3 nmol min
−1
(mg protein)
−1
. Benzoic acid competitively inhibited fluorescein transport, suggesting that fluorescein is transported by a monocarboxylic acid transporter (MCT). Antioxidative polyphenolic compounds such as ferulic acid from dietary sources, competitively inhibited the permeation of fluorescein. These compounds probably share a transport carrier with fluorescein. Measurement of the effects of phenolic acids on fluorescein transport across Caco-2 monolayers would be a useful way to evaluate the intestinal absorption or bioavailability of dietary phenolic acids.</description><subject>availability</subject><subject>Benzoic Acid - pharmacology</subject><subject>BIOAVAILABILITY</subject><subject>Biological and medical sciences</subject><subject>Biological Availability</subject><subject>Caco-2 Cells</subject><subject>Cell physiology</subject><subject>CELLS</subject><subject>Coumaric Acids - pharmacology</subject><subject>Drug Interactions</subject><subject>Electric Impedance</subject><subject>Epithelial Cells - metabolism</subject><subject>EPITHELIUM</subject><subject>ferulic acid</subject><subject>fluorescein</subject><subject>Fluorescein - pharmacokinetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroxybenzoates - pharmacokinetics</subject><subject>Intestinal Absorption - drug effects</subject><subject>Intestinal Mucosa - cytology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>INTESTINES</subject><subject>Isoquinolines - metabolism</subject><subject>Membrane and intracellular transports</subject><subject>Molecular and cellular biology</subject><subject>monocarboxylic acid transporter</subject><subject>Monocarboxylic Acid Transporters - metabolism</subject><subject>PERMEABILITY</subject><subject>PHENOLIC ACIDS</subject><subject>Tight Junctions - drug effects</subject><subject>Tight Junctions - physiology</subject><issn>0916-8451</issn><issn>1347-6947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc9rHCEUx6U0NJu0p55bhJJTma2OjrMew9JfIdAe0rO8cbRrcMepOlv2z8h_XKezJTkUHojy-b73-IjQa0rWtG7ph67r1kKsa87lM7SijLeVkLx9jlZEUlFteEPP0UVK94SUh4a-QOe0boiQG7FCD3cRhmRGl3fGO_A4z_cxxIyDxdZPIZqkjRtwqS3oUNVYG-_xPgzBw9HEhGHo8ZTMHEiT3j1pscQOLseAzQH8BNmFYQbHnSl5pzFo12M4gPPQOe_y8SU6s-CTeXU6L9GPTx_vtl-q22-fv26vbyvNaZ0rCg2hvNd9LRspLTVdy3omuZWm63QvWAu2K4y0dW81kaZthd4wJkRDNIBll-jd0neM4ddkUlb3YYpDGaloUcl509SkUO8XSseQUjRWjdHtIR4VJWrWr4p-JYSa9Rf67ann1O1N_8iefBfg6gRA0uBtMaVdeuQ4L1_E5rFi4dxgQ9zD7xB9rzIcfYj_Quz_G7xZghaCgp-xcDffa0LY32rYHwC3q4k</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Konishi, Y. (Kirin Brewery Co. Ltd., Takasaki, Gunma (Japan). Research Labs.)</creator><creator>Hagiwara, K</creator><creator>Shimizu, M</creator><general>Japan Society for Bioscience, Biotechnology, and Agrochemistry</general><general>Japan Society for Bioscience Biotechnology and Agrochemistry</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20021101</creationdate><title>Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability</title><author>Konishi, Y. (Kirin Brewery Co. Ltd., Takasaki, Gunma (Japan). Research Labs.) ; Hagiwara, K ; Shimizu, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-1a5014dcd29599f1eb73d394f9ebbcd637afba509f2dfc09e776c8336650caaf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>availability</topic><topic>Benzoic Acid - pharmacology</topic><topic>BIOAVAILABILITY</topic><topic>Biological and medical sciences</topic><topic>Biological Availability</topic><topic>Caco-2 Cells</topic><topic>Cell physiology</topic><topic>CELLS</topic><topic>Coumaric Acids - pharmacology</topic><topic>Drug Interactions</topic><topic>Electric Impedance</topic><topic>Epithelial Cells - metabolism</topic><topic>EPITHELIUM</topic><topic>ferulic acid</topic><topic>fluorescein</topic><topic>Fluorescein - pharmacokinetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroxybenzoates - pharmacokinetics</topic><topic>Intestinal Absorption - drug effects</topic><topic>Intestinal Mucosa - cytology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>INTESTINES</topic><topic>Isoquinolines - metabolism</topic><topic>Membrane and intracellular transports</topic><topic>Molecular and cellular biology</topic><topic>monocarboxylic acid transporter</topic><topic>Monocarboxylic Acid Transporters - metabolism</topic><topic>PERMEABILITY</topic><topic>PHENOLIC ACIDS</topic><topic>Tight Junctions - drug effects</topic><topic>Tight Junctions - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Konishi, Y. (Kirin Brewery Co. Ltd., Takasaki, Gunma (Japan). Research Labs.)</creatorcontrib><creatorcontrib>Hagiwara, K</creatorcontrib><creatorcontrib>Shimizu, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bioscience, biotechnology, and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Konishi, Y. (Kirin Brewery Co. Ltd., Takasaki, Gunma (Japan). Research Labs.)</au><au>Hagiwara, K</au><au>Shimizu, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability</atitle><jtitle>Bioscience, biotechnology, and biochemistry</jtitle><addtitle>Biosci Biotechnol Biochem</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>66</volume><issue>11</issue><spage>2449</spage><epage>2457</epage><pages>2449-2457</pages><issn>0916-8451</issn><eissn>1347-6947</eissn><abstract>Fluorescein is a marker-dye customary applied to the evaluation of tight-junctional permeability of epithelial cell monolayers. However, the true mechanism for the permeation has not been elucidated. Transepithelial transport of fluorescein in Caco-2 cell monolayers was therefore examined. Fluorescein transport was dependent on pH, and in a vectorical way in the apical-basolateral direction, but it was independent of the tight-junctional permeability of monolayers of these human intestinal cells. The permeation of fluorescein was concentration-dependent and saturable; the Michaelis constant was 7.7 mM and the maximum velocity was 40.3 nmol min
−1
(mg protein)
−1
. Benzoic acid competitively inhibited fluorescein transport, suggesting that fluorescein is transported by a monocarboxylic acid transporter (MCT). Antioxidative polyphenolic compounds such as ferulic acid from dietary sources, competitively inhibited the permeation of fluorescein. These compounds probably share a transport carrier with fluorescein. Measurement of the effects of phenolic acids on fluorescein transport across Caco-2 monolayers would be a useful way to evaluate the intestinal absorption or bioavailability of dietary phenolic acids.</abstract><cop>Tokyo</cop><pub>Japan Society for Bioscience, Biotechnology, and Agrochemistry</pub><pmid>12506986</pmid><doi>10.1271/bbb.66.2449</doi><tpages>9</tpages></addata></record> |
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| ispartof | Bioscience, biotechnology, and biochemistry, 2002-11, Vol.66 (11), p.2449-2457 |
| issn | 0916-8451 1347-6947 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_14409130 |
| source | J-STAGE Free; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Freely Accessible Japanese Titles; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | availability Benzoic Acid - pharmacology BIOAVAILABILITY Biological and medical sciences Biological Availability Caco-2 Cells Cell physiology CELLS Coumaric Acids - pharmacology Drug Interactions Electric Impedance Epithelial Cells - metabolism EPITHELIUM ferulic acid fluorescein Fluorescein - pharmacokinetics Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration Hydroxybenzoates - pharmacokinetics Intestinal Absorption - drug effects Intestinal Mucosa - cytology Intestinal Mucosa - metabolism INTESTINES Isoquinolines - metabolism Membrane and intracellular transports Molecular and cellular biology monocarboxylic acid transporter Monocarboxylic Acid Transporters - metabolism PERMEABILITY PHENOLIC ACIDS Tight Junctions - drug effects Tight Junctions - physiology |
| title | Transepithelial transport of fluorescein in Caco-2 cell monolayers and use of such transport in in vitro evaluation of phenolic acid availability |
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