Induction of Mucous Cell Metaplasia in the Middle Ear of Rats Using a Three-step Method: An Improved Model for Otitis Media with Mucoid Effusion
Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especiall...
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| Veröffentlicht in: | Acta oto-laryngologica 2002-03, Vol.122 (2), p.153-160 |
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| creator | Tsuboi, Yasuhiro Kim, Youngki Giebink, G. Scott Le, Chap Paparella, Michael M. Chen, Nengqian Schachern, Patricia A. Juhn, Steven K. Lin, Jizhen |
| description | Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 10 7 colony-forming units (CFU) ear or Haemophilus influenzae at 5 ½ 10 7 CFU ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 10 7 CFU ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart. |
| doi_str_mv | 10.1080/00016480252814153 |
| format | Article |
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Scott ; Le, Chap ; Paparella, Michael M. ; Chen, Nengqian ; Schachern, Patricia A. ; Juhn, Steven K. ; Lin, Jizhen</creator><creatorcontrib>Tsuboi, Yasuhiro ; Kim, Youngki ; Giebink, G. Scott ; Le, Chap ; Paparella, Michael M. ; Chen, Nengqian ; Schachern, Patricia A. ; Juhn, Steven K. ; Lin, Jizhen</creatorcontrib><description>Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 10 7 colony-forming units (CFU) ear or Haemophilus influenzae at 5 ½ 10 7 CFU ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 10 7 CFU ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart.</description><identifier>ISSN: 0001-6489</identifier><identifier>EISSN: 1651-2251</identifier><identifier>DOI: 10.1080/00016480252814153</identifier><identifier>PMID: 11936906</identifier><identifier>CODEN: AOLAAJ</identifier><language>eng</language><publisher>Stockholm: Informa UK Ltd</publisher><subject>Animals ; Bacterial diseases ; Biological and medical sciences ; Carbohydrate Metabolism ; Cell ; Disease Models, Animal ; Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology ; Ear, Middle - pathology ; Effusion ; Ent and stomatologic bacterial diseases ; Eustachian Tube ; Haemophilus influenzae ; Human bacterial diseases ; Humans ; Infectious diseases ; Media ; Medical sciences ; Metaplasia ; Middle ; Mucoid ; Mucous ; Mucous Membrane - cytology ; Mucous Membrane - pathology ; Non tumoral diseases ; Otitis ; Otitis Media with Effusion - metabolism ; Otitis Media with Effusion - pathology ; Otorhinolaryngology. Stomatology ; Rats ; Rats, Sprague-Dawley ; Streptococcus pneumoniae ; Time Factors</subject><ispartof>Acta oto-laryngologica, 2002-03, Vol.122 (2), p.153-160</ispartof><rights>2002 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2002</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-6c530491a117eeeee471affac7ed858ab07d62ed39ff1969dc9c5df4fd9de7a43</citedby><cites>FETCH-LOGICAL-c463t-6c530491a117eeeee471affac7ed858ab07d62ed39ff1969dc9c5df4fd9de7a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00016480252814153$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00016480252814153$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13731175$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11936906$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsuboi, Yasuhiro</creatorcontrib><creatorcontrib>Kim, Youngki</creatorcontrib><creatorcontrib>Giebink, G. Scott</creatorcontrib><creatorcontrib>Le, Chap</creatorcontrib><creatorcontrib>Paparella, Michael M.</creatorcontrib><creatorcontrib>Chen, Nengqian</creatorcontrib><creatorcontrib>Schachern, Patricia A.</creatorcontrib><creatorcontrib>Juhn, Steven K.</creatorcontrib><creatorcontrib>Lin, Jizhen</creatorcontrib><title>Induction of Mucous Cell Metaplasia in the Middle Ear of Rats Using a Three-step Method: An Improved Model for Otitis Media with Mucoid Effusion</title><title>Acta oto-laryngologica</title><addtitle>Acta Otolaryngol</addtitle><description>Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 10 7 colony-forming units (CFU) ear or Haemophilus influenzae at 5 ½ 10 7 CFU ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 10 7 CFU ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart.</description><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Metabolism</subject><subject>Cell</subject><subject>Disease Models, Animal</subject><subject>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</subject><subject>Ear, Middle - pathology</subject><subject>Effusion</subject><subject>Ent and stomatologic bacterial diseases</subject><subject>Eustachian Tube</subject><subject>Haemophilus influenzae</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Media</subject><subject>Medical sciences</subject><subject>Metaplasia</subject><subject>Middle</subject><subject>Mucoid</subject><subject>Mucous</subject><subject>Mucous Membrane - cytology</subject><subject>Mucous Membrane - pathology</subject><subject>Non tumoral diseases</subject><subject>Otitis</subject><subject>Otitis Media with Effusion - metabolism</subject><subject>Otitis Media with Effusion - pathology</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Streptococcus pneumoniae</subject><subject>Time Factors</subject><issn>0001-6489</issn><issn>1651-2251</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0EokPhAdggb2AXsJM4iYFNNRpgpI4qoXYd3drXxJUTD7ZD1bfgkXGYQRVCKt5Ylr9z7s8h5CVnbznr2DvGGG_qjpWi7HjNRfWIrHgjeFGWgj8mq-W_yIA8Ic9ivFmeshNPyQnnsmoka1bk53bSs0rWT9QbupuVnyNdo3N0hwn2DqIFaieaBqQ7q7VDuoGwsF8hRXoV7fSNAr0cAmIRE-4X3eD1e3o20e24D_4HarrzGh01PtCLZJONGdLZ99am4XdNq-nGmDnmNp6TJwZcxBfH-5Rcfdpcrr8U5xeft-uz80LVTZWKRomK1ZID5y0up245GAOqRd2JDq5Zq5sSdSWN4bKRWkkltKmNlhpbqKtT8ubgm1v8PmNM_WijyoPDhHkHfctFx-qq_C9Y8rLuynZx5AdQBR9jQNPvgx0h3PWc9Ute_T95Zc2ro_l8PaK-VxwDysDrIwBRgTMBJmXjPVe1Vd6AyNzHA2envOYRbn1wuk9w53z4I6oe6uPDX_IBwaVBQcD-xs9hykk8MMUv_7bC7w</recordid><startdate>20020301</startdate><enddate>20020301</enddate><creator>Tsuboi, Yasuhiro</creator><creator>Kim, Youngki</creator><creator>Giebink, G. Scott</creator><creator>Le, Chap</creator><creator>Paparella, Michael M.</creator><creator>Chen, Nengqian</creator><creator>Schachern, Patricia A.</creator><creator>Juhn, Steven K.</creator><creator>Lin, Jizhen</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>20020301</creationdate><title>Induction of Mucous Cell Metaplasia in the Middle Ear of Rats Using a Three-step Method: An Improved Model for Otitis Media with Mucoid Effusion</title><author>Tsuboi, Yasuhiro ; Kim, Youngki ; Giebink, G. Scott ; Le, Chap ; Paparella, Michael M. ; Chen, Nengqian ; Schachern, Patricia A. ; Juhn, Steven K. ; Lin, Jizhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-6c530491a117eeeee471affac7ed858ab07d62ed39ff1969dc9c5df4fd9de7a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Metabolism</topic><topic>Cell</topic><topic>Disease Models, Animal</topic><topic>Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology</topic><topic>Ear, Middle - pathology</topic><topic>Effusion</topic><topic>Ent and stomatologic bacterial diseases</topic><topic>Eustachian Tube</topic><topic>Haemophilus influenzae</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Media</topic><topic>Medical sciences</topic><topic>Metaplasia</topic><topic>Middle</topic><topic>Mucoid</topic><topic>Mucous</topic><topic>Mucous Membrane - cytology</topic><topic>Mucous Membrane - pathology</topic><topic>Non tumoral diseases</topic><topic>Otitis</topic><topic>Otitis Media with Effusion - metabolism</topic><topic>Otitis Media with Effusion - pathology</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Streptococcus pneumoniae</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsuboi, Yasuhiro</creatorcontrib><creatorcontrib>Kim, Youngki</creatorcontrib><creatorcontrib>Giebink, G. Scott</creatorcontrib><creatorcontrib>Le, Chap</creatorcontrib><creatorcontrib>Paparella, Michael M.</creatorcontrib><creatorcontrib>Chen, Nengqian</creatorcontrib><creatorcontrib>Schachern, Patricia A.</creatorcontrib><creatorcontrib>Juhn, Steven K.</creatorcontrib><creatorcontrib>Lin, Jizhen</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Acta oto-laryngologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsuboi, Yasuhiro</au><au>Kim, Youngki</au><au>Giebink, G. Scott</au><au>Le, Chap</au><au>Paparella, Michael M.</au><au>Chen, Nengqian</au><au>Schachern, Patricia A.</au><au>Juhn, Steven K.</au><au>Lin, Jizhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Mucous Cell Metaplasia in the Middle Ear of Rats Using a Three-step Method: An Improved Model for Otitis Media with Mucoid Effusion</atitle><jtitle>Acta oto-laryngologica</jtitle><addtitle>Acta Otolaryngol</addtitle><date>2002-03-01</date><risdate>2002</risdate><volume>122</volume><issue>2</issue><spage>153</spage><epage>160</epage><pages>153-160</pages><issn>0001-6489</issn><eissn>1651-2251</eissn><coden>AOLAAJ</coden><abstract>Otitis media with mucoid effusion, characterized by mucous cell metaplasia in the middle ear cleft and thick fluid accumulation in the middle ear cavity, is a common otological disease that frequently affects young children. Multiple factors are involved in the development of this disease, especially middle ear infection and Eustachian tube dysfunction. In this study, in order to induce otitis media with effusion in rats, we introduced a three-step method, namely inoculation of Streptococcus pneumoniae at 10 7 colony-forming units (CFU) ear or Haemophilus influenzae at 5 ½ 10 7 CFU ear into the middle ear cavity twice at 2-week intervals, followed by Eustachian tube obstruction (ETO) for 4 and 8 weeks. Animals inoculated with phosphate-buffered saline (PBS) twice in the same manner followed by ETO served as controls. Middle ear effusion and mucosa were harvested for evaluation of carbohydrate concentrations and mucous cell density, respectively. We found that rats inoculated with S. pneumoniae twice, followed by ETO at 8 weeks, yielded the highest carbohydrate concentration in middle ear effusion and the highest goblet cell density in the middle ear cavity compared to the H. influenzae and PBS groups. It is tentatively concluded that inoculation of S. pneumoniae at 10 7 CFU ear into the middle ear cavity of rats twice at 2-week intervals, followed by ETO for 8 weeks, is a promising animal model for otitis media with mucoid effusion which may be valuable for studying the human counterpart.</abstract><cop>Stockholm</cop><pub>Informa UK Ltd</pub><pmid>11936906</pmid><doi>10.1080/00016480252814153</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Animals Bacterial diseases Biological and medical sciences Carbohydrate Metabolism Cell Disease Models, Animal Ear, auditive nerve, cochleovestibular tract, facial nerve: diseases, semeiology Ear, Middle - pathology Effusion Ent and stomatologic bacterial diseases Eustachian Tube Haemophilus influenzae Human bacterial diseases Humans Infectious diseases Media Medical sciences Metaplasia Middle Mucoid Mucous Mucous Membrane - cytology Mucous Membrane - pathology Non tumoral diseases Otitis Otitis Media with Effusion - metabolism Otitis Media with Effusion - pathology Otorhinolaryngology. Stomatology Rats Rats, Sprague-Dawley Streptococcus pneumoniae Time Factors |
| title | Induction of Mucous Cell Metaplasia in the Middle Ear of Rats Using a Three-step Method: An Improved Model for Otitis Media with Mucoid Effusion |
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