Gender-specific K+-channel contribution to adenosine-induced relaxation in coronary arterioles
Department of Biomedical Sciences, College of Veterinary Medicine, and Dalton Cardiovascular Research Center, University of Missouri, Columbia, Missouri 65211 We examined the contribution of K + -channel activity on basal tone and adenosine-mediated relaxation of coronary arterioles isolated from se...
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| Veröffentlicht in: | Journal of applied physiology (1985) 2002-02, Vol.92 (2), p.550-558 |
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| container_title | Journal of applied physiology (1985) |
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| creator | Heaps, Cristine L Bowles, Douglas K |
| description | Department of Biomedical Sciences, College of Veterinary Medicine,
and Dalton Cardiovascular Research Center, University of Missouri,
Columbia, Missouri 65211
We examined the contribution of
K + -channel activity on basal tone and adenosine-mediated
relaxation of coronary arterioles isolated from sexually mature male
and female miniature swine. Arterioles (~100-200 µm
ID) isolated from the apical region of the heart were cannulated and
studied using videodimensional analysis under constant intraluminal
pressure. Coronary arterioles from male and female pigs demonstrated
similar levels of basal tone and reductions in basal diameter in
response to the K + -channel blockers 4-aminopyridine (4-AP;
1 mM), tetraethylammonium (1 mM), and glibenclamide (Glib; 10 µM),
with 4-AP producing significantly greater constriction than
tetraethylammonium or Glib. After endothelin-induced preconstriction,
relaxation responses to adenosine were not significantly different
between coronary arterioles of male and female pigs. Inhibition of
4-AP-sensitive channels significantly impaired adenosine-mediated relaxation in arterioles from male but not female pigs. However, inhibition of K + channels with iberiotoxin (100 nM) or Glib
had no effect on adenosine-induced relaxation in either sex. Results
obtained in the presence of nitric oxide synthase inhibition suggest a
potential interaction of 4-AP-sensitive channels and nitric oxide at
low adenosine concentrations. In conclusion, our data indicate that
4-AP-sensitive channels 1 ) contribute significantly to basal
tone in coronary arterioles of both male and female pigs, 2 )
contribute to adenosine-mediated relaxation in male but not female
pigs, and 3 ) can contribute to adenosine-induced relaxation
independent of nitric oxide production in male pigs. These data are
consistent with a significant role for voltage-dependent K +
channels in adenosine-mediated relaxation of coronary arterioles from males.
potassium channel; smooth muscle; endothelium; nitric oxide
synthase; endothelin |
| doi_str_mv | 10.1152/japplphysiol.00566.2001 |
| format | Article |
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and Dalton Cardiovascular Research Center, University of Missouri,
Columbia, Missouri 65211
We examined the contribution of
K + -channel activity on basal tone and adenosine-mediated
relaxation of coronary arterioles isolated from sexually mature male
and female miniature swine. Arterioles (~100-200 µm
ID) isolated from the apical region of the heart were cannulated and
studied using videodimensional analysis under constant intraluminal
pressure. Coronary arterioles from male and female pigs demonstrated
similar levels of basal tone and reductions in basal diameter in
response to the K + -channel blockers 4-aminopyridine (4-AP;
1 mM), tetraethylammonium (1 mM), and glibenclamide (Glib; 10 µM),
with 4-AP producing significantly greater constriction than
tetraethylammonium or Glib. After endothelin-induced preconstriction,
relaxation responses to adenosine were not significantly different
between coronary arterioles of male and female pigs. Inhibition of
4-AP-sensitive channels significantly impaired adenosine-mediated relaxation in arterioles from male but not female pigs. However, inhibition of K + channels with iberiotoxin (100 nM) or Glib
had no effect on adenosine-induced relaxation in either sex. Results
obtained in the presence of nitric oxide synthase inhibition suggest a
potential interaction of 4-AP-sensitive channels and nitric oxide at
low adenosine concentrations. In conclusion, our data indicate that
4-AP-sensitive channels 1 ) contribute significantly to basal
tone in coronary arterioles of both male and female pigs, 2 )
contribute to adenosine-mediated relaxation in male but not female
pigs, and 3 ) can contribute to adenosine-induced relaxation
independent of nitric oxide production in male pigs. These data are
consistent with a significant role for voltage-dependent K +
channels in adenosine-mediated relaxation of coronary arterioles from males.
potassium channel; smooth muscle; endothelium; nitric oxide
synthase; endothelin</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/japplphysiol.00566.2001</identifier><identifier>PMID: 11796663</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Adenosine - pharmacology ; Animals ; Arterioles - drug effects ; Arterioles - physiology ; Biological and medical sciences ; Coronary vessels ; Coronary Vessels - drug effects ; Coronary Vessels - physiology ; Dose-Response Relationship, Drug ; Electric Conductivity ; Epoprostenol - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Gender ; Heart ; Hogs ; In Vitro Techniques ; Male ; Muscle, Smooth, Vascular - metabolism ; Nitric Oxide - physiology ; Osmolar Concentration ; Peptides - pharmacology ; Potassium ; Potassium Channel Blockers - pharmacology ; Potassium Channels - drug effects ; Potassium Channels - physiology ; Sex Characteristics ; Swine, Miniature ; Tetraethylammonium - pharmacology ; Vasodilation - physiology ; Vasodilator Agents - pharmacology ; Vertebrates: cardiovascular system</subject><ispartof>Journal of applied physiology (1985), 2002-02, Vol.92 (2), p.550-558</ispartof><rights>2002 INIST-CNRS</rights><rights>Copyright American Physiological Society Feb 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-322185d766e80ac76c9ee4a3d2fb195e4b10d146bb4d37793f5be83e734d04d33</citedby><cites>FETCH-LOGICAL-c442t-322185d766e80ac76c9ee4a3d2fb195e4b10d146bb4d37793f5be83e734d04d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13480738$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11796663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heaps, Cristine L</creatorcontrib><creatorcontrib>Bowles, Douglas K</creatorcontrib><title>Gender-specific K+-channel contribution to adenosine-induced relaxation in coronary arterioles</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>Department of Biomedical Sciences, College of Veterinary Medicine,
and Dalton Cardiovascular Research Center, University of Missouri,
Columbia, Missouri 65211
We examined the contribution of
K + -channel activity on basal tone and adenosine-mediated
relaxation of coronary arterioles isolated from sexually mature male
and female miniature swine. Arterioles (~100-200 µm
ID) isolated from the apical region of the heart were cannulated and
studied using videodimensional analysis under constant intraluminal
pressure. Coronary arterioles from male and female pigs demonstrated
similar levels of basal tone and reductions in basal diameter in
response to the K + -channel blockers 4-aminopyridine (4-AP;
1 mM), tetraethylammonium (1 mM), and glibenclamide (Glib; 10 µM),
with 4-AP producing significantly greater constriction than
tetraethylammonium or Glib. After endothelin-induced preconstriction,
relaxation responses to adenosine were not significantly different
between coronary arterioles of male and female pigs. Inhibition of
4-AP-sensitive channels significantly impaired adenosine-mediated relaxation in arterioles from male but not female pigs. However, inhibition of K + channels with iberiotoxin (100 nM) or Glib
had no effect on adenosine-induced relaxation in either sex. Results
obtained in the presence of nitric oxide synthase inhibition suggest a
potential interaction of 4-AP-sensitive channels and nitric oxide at
low adenosine concentrations. In conclusion, our data indicate that
4-AP-sensitive channels 1 ) contribute significantly to basal
tone in coronary arterioles of both male and female pigs, 2 )
contribute to adenosine-mediated relaxation in male but not female
pigs, and 3 ) can contribute to adenosine-induced relaxation
independent of nitric oxide production in male pigs. These data are
consistent with a significant role for voltage-dependent K +
channels in adenosine-mediated relaxation of coronary arterioles from males.
potassium channel; smooth muscle; endothelium; nitric oxide
synthase; endothelin</description><subject>Adenosine - pharmacology</subject><subject>Animals</subject><subject>Arterioles - drug effects</subject><subject>Arterioles - physiology</subject><subject>Biological and medical sciences</subject><subject>Coronary vessels</subject><subject>Coronary Vessels - drug effects</subject><subject>Coronary Vessels - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electric Conductivity</subject><subject>Epoprostenol - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gender</subject><subject>Heart</subject><subject>Hogs</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Nitric Oxide - physiology</subject><subject>Osmolar Concentration</subject><subject>Peptides - pharmacology</subject><subject>Potassium</subject><subject>Potassium Channel Blockers - pharmacology</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - physiology</subject><subject>Sex Characteristics</subject><subject>Swine, Miniature</subject><subject>Tetraethylammonium - pharmacology</subject><subject>Vasodilation - physiology</subject><subject>Vasodilator Agents - pharmacology</subject><subject>Vertebrates: cardiovascular system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kF1rFDEUhoModq3-BR0E9UJmzXdmLqXYKha8qbeGTHKmmyWbjMkMdv-92e5KRfAqkPO8J28ehF4RvCZE0A9bM01h2uyLT2GNsZByTTEmj9CqTmlLJCaP0apTArdKdOoMPStlWwHOBXmKzghRvZSSrdCPK4gOclsmsH70tvn6vrUbEyOExqY4Zz8ss0-xmVNjHMRUfITWR7dYcE2GYO7M_dzHyucUTd43Js-QazMoz9GT0YQCL07nOfp--enm4nN7_e3qy8XH69ZyTueWUUo64ZSU0GFjlbQ9ADfM0XEgvQA-EOwIl8PAHVOqZ6MYoGOgGHe4XrFz9Pa4d8rp5wJl1jtfLIRgIqSlaEU47jmXFXz9D7hNS461m6a1hOCKHiB1hGxOpWQY9ZT9rv5ME6wP_vXf_vW9f33wX5MvT-uXYQfuIXcSXoE3J8AUa8KYTbS-PHCMd1ixrnLsyG387eaXz6BPr6Xbvb5cQriBu_lQo6eaaiGwntxYU-_-n6qw_kOz36_Gs5U</recordid><startdate>20020201</startdate><enddate>20020201</enddate><creator>Heaps, Cristine L</creator><creator>Bowles, Douglas K</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20020201</creationdate><title>Gender-specific K+-channel contribution to adenosine-induced relaxation in coronary arterioles</title><author>Heaps, Cristine L ; Bowles, Douglas K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-322185d766e80ac76c9ee4a3d2fb195e4b10d146bb4d37793f5be83e734d04d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adenosine - pharmacology</topic><topic>Animals</topic><topic>Arterioles - drug effects</topic><topic>Arterioles - physiology</topic><topic>Biological and medical sciences</topic><topic>Coronary vessels</topic><topic>Coronary Vessels - drug effects</topic><topic>Coronary Vessels - physiology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electric Conductivity</topic><topic>Epoprostenol - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gender</topic><topic>Heart</topic><topic>Hogs</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Nitric Oxide - physiology</topic><topic>Osmolar Concentration</topic><topic>Peptides - pharmacology</topic><topic>Potassium</topic><topic>Potassium Channel Blockers - pharmacology</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>Sex Characteristics</topic><topic>Swine, Miniature</topic><topic>Tetraethylammonium - pharmacology</topic><topic>Vasodilation - physiology</topic><topic>Vasodilator Agents - pharmacology</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heaps, Cristine L</creatorcontrib><creatorcontrib>Bowles, Douglas K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heaps, Cristine L</au><au>Bowles, Douglas K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gender-specific K+-channel contribution to adenosine-induced relaxation in coronary arterioles</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2002-02-01</date><risdate>2002</risdate><volume>92</volume><issue>2</issue><spage>550</spage><epage>558</epage><pages>550-558</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>Department of Biomedical Sciences, College of Veterinary Medicine,
and Dalton Cardiovascular Research Center, University of Missouri,
Columbia, Missouri 65211
We examined the contribution of
K + -channel activity on basal tone and adenosine-mediated
relaxation of coronary arterioles isolated from sexually mature male
and female miniature swine. Arterioles (~100-200 µm
ID) isolated from the apical region of the heart were cannulated and
studied using videodimensional analysis under constant intraluminal
pressure. Coronary arterioles from male and female pigs demonstrated
similar levels of basal tone and reductions in basal diameter in
response to the K + -channel blockers 4-aminopyridine (4-AP;
1 mM), tetraethylammonium (1 mM), and glibenclamide (Glib; 10 µM),
with 4-AP producing significantly greater constriction than
tetraethylammonium or Glib. After endothelin-induced preconstriction,
relaxation responses to adenosine were not significantly different
between coronary arterioles of male and female pigs. Inhibition of
4-AP-sensitive channels significantly impaired adenosine-mediated relaxation in arterioles from male but not female pigs. However, inhibition of K + channels with iberiotoxin (100 nM) or Glib
had no effect on adenosine-induced relaxation in either sex. Results
obtained in the presence of nitric oxide synthase inhibition suggest a
potential interaction of 4-AP-sensitive channels and nitric oxide at
low adenosine concentrations. In conclusion, our data indicate that
4-AP-sensitive channels 1 ) contribute significantly to basal
tone in coronary arterioles of both male and female pigs, 2 )
contribute to adenosine-mediated relaxation in male but not female
pigs, and 3 ) can contribute to adenosine-induced relaxation
independent of nitric oxide production in male pigs. These data are
consistent with a significant role for voltage-dependent K +
channels in adenosine-mediated relaxation of coronary arterioles from males.
potassium channel; smooth muscle; endothelium; nitric oxide
synthase; endothelin</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>11796663</pmid><doi>10.1152/japplphysiol.00566.2001</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 8750-7587 |
| ispartof | Journal of applied physiology (1985), 2002-02, Vol.92 (2), p.550-558 |
| issn | 8750-7587 1522-1601 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_13480738 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adenosine - pharmacology Animals Arterioles - drug effects Arterioles - physiology Biological and medical sciences Coronary vessels Coronary Vessels - drug effects Coronary Vessels - physiology Dose-Response Relationship, Drug Electric Conductivity Epoprostenol - physiology Female Fundamental and applied biological sciences. Psychology Gender Heart Hogs In Vitro Techniques Male Muscle, Smooth, Vascular - metabolism Nitric Oxide - physiology Osmolar Concentration Peptides - pharmacology Potassium Potassium Channel Blockers - pharmacology Potassium Channels - drug effects Potassium Channels - physiology Sex Characteristics Swine, Miniature Tetraethylammonium - pharmacology Vasodilation - physiology Vasodilator Agents - pharmacology Vertebrates: cardiovascular system |
| title | Gender-specific K+-channel contribution to adenosine-induced relaxation in coronary arterioles |
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