Metal speciation in xylem exudate of Lycopersicon esculentum Mill-technetium
This paper deals with a new analytical approach for studying metal speciation in the xylem, as applied to the nuclear waste metal technetium (⁹⁹Tc) in tomato plants (Lycopersicon esculentum Mill, cv. Tiny Tim). Special attention was paid to sampling protocols and sample handling so that metal oxidat...
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| Veröffentlicht in: | Plant and soil 1999, Vol.212 (2), p.165-173 |
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| container_title | Plant and soil |
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| creator | Krijger, G.C. (Delft Univ. of Technology (Netherlands). Dept. of Radiochemistry) Vliet, P.M. Van Wolterbeek, H.T |
| description | This paper deals with a new analytical approach for studying metal speciation in the xylem, as applied to the nuclear waste metal technetium (⁹⁹Tc) in tomato plants (Lycopersicon esculentum Mill, cv. Tiny Tim). Special attention was paid to sampling protocols and sample handling so that metal oxidation/reduction processes during analytical procedures did not influence the results. Marker Tc-ligand complexes were synthesized in vitro (Tc-cysteine, Tc-histidine, Tc-citrate, and Tc-nicotianamine) and sampling was performed under both argon and atmospheric conditions. Analyses were carried out using paper chromatography, gel electrophoresis (PAGE), and reversed phase ion-pair chromatography (RP-IPC). The results show Tc to be present predominantly in the TcO₄⁻ form in the xylem, indicating transport similarities with molybdenum and chromium. Probably, the approach used is also valuable in other metal-speciation studies in the xylem. |
| format | Article |
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The results show Tc to be present predominantly in the TcO₄⁻ form in the xylem, indicating transport similarities with molybdenum and chromium. Probably, the approach used is also valuable in other metal-speciation studies in the xylem.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>CODEN: PLSOA2</identifier><language>eng</language><publisher>Dordrecht: Kluwer Academic Publishers</publisher><subject>Absorption. Translocation of ions and substances. Permeability ; Agronomy. Soil science and plant productions ; Amino acids ; Argon ; Biological and medical sciences ; Carboxylic acids ; Economic plant physiology ; EXSUDAT ; EXUDADOS ; EXUDATES ; Fundamental and applied biological sciences. Psychology ; LIGAND ; LIGANDOS ; LIGANDS ; LYCOPERSICON ESCULENTUM ; Non agrochemicals pollutants ; NUTRIENT TRANSPORT ; Nutrition. Photosynthesis. Respiration. Metabolism ; Paper chromatography ; Phytopathology. Animal pests. Plant and forest protection ; Plants ; Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors ; Pollution effects. 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(Delft Univ. of Technology (Netherlands). Dept. of Radiochemistry)</creatorcontrib><creatorcontrib>Vliet, P.M. Van</creatorcontrib><creatorcontrib>Wolterbeek, H.T</creatorcontrib><title>Metal speciation in xylem exudate of Lycopersicon esculentum Mill-technetium</title><title>Plant and soil</title><description>This paper deals with a new analytical approach for studying metal speciation in the xylem, as applied to the nuclear waste metal technetium (⁹⁹Tc) in tomato plants (Lycopersicon esculentum Mill, cv. Tiny Tim). Special attention was paid to sampling protocols and sample handling so that metal oxidation/reduction processes during analytical procedures did not influence the results. Marker Tc-ligand complexes were synthesized in vitro (Tc-cysteine, Tc-histidine, Tc-citrate, and Tc-nicotianamine) and sampling was performed under both argon and atmospheric conditions. Analyses were carried out using paper chromatography, gel electrophoresis (PAGE), and reversed phase ion-pair chromatography (RP-IPC). The results show Tc to be present predominantly in the TcO₄⁻ form in the xylem, indicating transport similarities with molybdenum and chromium. Probably, the approach used is also valuable in other metal-speciation studies in the xylem.</description><subject>Absorption. Translocation of ions and substances. Permeability</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Amino acids</subject><subject>Argon</subject><subject>Biological and medical sciences</subject><subject>Carboxylic acids</subject><subject>Economic plant physiology</subject><subject>EXSUDAT</subject><subject>EXUDADOS</subject><subject>EXUDATES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>LIGAND</subject><subject>LIGANDOS</subject><subject>LIGANDS</subject><subject>LYCOPERSICON ESCULENTUM</subject><subject>Non agrochemicals pollutants</subject><subject>NUTRIENT TRANSPORT</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>Paper chromatography</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plants</subject><subject>Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors</subject><subject>Pollution effects. Side effects of agrochemicals</subject><subject>Short Communication</subject><subject>Speciation</subject><subject>Species</subject><subject>TECHNETIUM</subject><subject>TECNECIO</subject><subject>TRANSPORT DES SUBSTANCES NUTRITIVES</subject><subject>TRANSPORTE DE NUTRIENTES</subject><subject>XILEMA</subject><subject>XYLEM</subject><subject>XYLEME</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNpFjktLAzEUhYMoWKs_QcjC7UBek0yWUnzBVDcK7so1c6MpmQeTDLT_3iktejaXw_k495yRBS-NLEom9TlZMCZFwYz9vCRXKW3ZwXO9IPUaM0SaBnQBcug7Gjq620dsKe6mBjLS3tN67_oBxxTcDGByU8QuTy1dhxiLjO6nwxym9ppceIgJb053ST4eH95Xz0X99vSyuq8LL4TOhcWSg1a-QiY8dxIar6TmjW3AOwbAcd7GAb-4csoYVVpmZOMk16KxEr1ckrtj7wDJQfQjdC6kzTCGFsb9hvOKK6Nm7PaIbVPux79YCausrsx_7qHfwPc4V7zWgh00v6rkL1SmXpk</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Krijger, G.C. (Delft Univ. of Technology (Netherlands). 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Soil science and plant productions</topic><topic>Amino acids</topic><topic>Argon</topic><topic>Biological and medical sciences</topic><topic>Carboxylic acids</topic><topic>Economic plant physiology</topic><topic>EXSUDAT</topic><topic>EXUDADOS</topic><topic>EXUDATES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>LIGAND</topic><topic>LIGANDOS</topic><topic>LIGANDS</topic><topic>LYCOPERSICON ESCULENTUM</topic><topic>Non agrochemicals pollutants</topic><topic>NUTRIENT TRANSPORT</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>Paper chromatography</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plants</topic><topic>Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors</topic><topic>Pollution effects. Side effects of agrochemicals</topic><topic>Short Communication</topic><topic>Speciation</topic><topic>Species</topic><topic>TECHNETIUM</topic><topic>TECNECIO</topic><topic>TRANSPORT DES SUBSTANCES NUTRITIVES</topic><topic>TRANSPORTE DE NUTRIENTES</topic><topic>XILEMA</topic><topic>XYLEM</topic><topic>XYLEME</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krijger, G.C. (Delft Univ. of Technology (Netherlands). Dept. of Radiochemistry)</creatorcontrib><creatorcontrib>Vliet, P.M. Van</creatorcontrib><creatorcontrib>Wolterbeek, H.T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krijger, G.C. (Delft Univ. of Technology (Netherlands). Dept. of Radiochemistry)</au><au>Vliet, P.M. Van</au><au>Wolterbeek, H.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metal speciation in xylem exudate of Lycopersicon esculentum Mill-technetium</atitle><jtitle>Plant and soil</jtitle><date>1999</date><risdate>1999</risdate><volume>212</volume><issue>2</issue><spage>165</spage><epage>173</epage><pages>165-173</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><coden>PLSOA2</coden><abstract>This paper deals with a new analytical approach for studying metal speciation in the xylem, as applied to the nuclear waste metal technetium (⁹⁹Tc) in tomato plants (Lycopersicon esculentum Mill, cv. Tiny Tim). Special attention was paid to sampling protocols and sample handling so that metal oxidation/reduction processes during analytical procedures did not influence the results. Marker Tc-ligand complexes were synthesized in vitro (Tc-cysteine, Tc-histidine, Tc-citrate, and Tc-nicotianamine) and sampling was performed under both argon and atmospheric conditions. Analyses were carried out using paper chromatography, gel electrophoresis (PAGE), and reversed phase ion-pair chromatography (RP-IPC). The results show Tc to be present predominantly in the TcO₄⁻ form in the xylem, indicating transport similarities with molybdenum and chromium. Probably, the approach used is also valuable in other metal-speciation studies in the xylem.</abstract><cop>Dordrecht</cop><pub>Kluwer Academic Publishers</pub><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0032-079X |
| ispartof | Plant and soil, 1999, Vol.212 (2), p.165-173 |
| issn | 0032-079X 1573-5036 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_1181474 |
| source | SpringerNature Journals; JSTOR Archive Collection A-Z Listing |
| subjects | Absorption. Translocation of ions and substances. Permeability Agronomy. Soil science and plant productions Amino acids Argon Biological and medical sciences Carboxylic acids Economic plant physiology EXSUDAT EXUDADOS EXUDATES Fundamental and applied biological sciences. Psychology LIGAND LIGANDOS LIGANDS LYCOPERSICON ESCULENTUM Non agrochemicals pollutants NUTRIENT TRANSPORT Nutrition. Photosynthesis. Respiration. Metabolism Paper chromatography Phytopathology. Animal pests. Plant and forest protection Plants Pollution effects and side effects of agrochemicals on crop plants and forest trees. Other anthropogenic factors Pollution effects. Side effects of agrochemicals Short Communication Speciation Species TECHNETIUM TECNECIO TRANSPORT DES SUBSTANCES NUTRITIVES TRANSPORTE DE NUTRIENTES XILEMA XYLEM XYLEME |
| title | Metal speciation in xylem exudate of Lycopersicon esculentum Mill-technetium |
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