MO1-18-2Analysis of metastatic foci using in house Gastric 58 SMG panel of 351,050 nt and d/pMMR status by IHC of MMR proteins
Abstract Background The clonality of primary tumors is well elucidated. In contrast, the relation to metastatic foci is not well understood. We examined genomic clonality of primary tumors and metastatic foci. Method We studied 144 foci (34 primaries, 104 lymph nodes, 6 organ mets) from 10 patients...
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| Veröffentlicht in: | Annals of oncology 2019-10, Vol.30 (Supplement_6) |
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| creator | Hada, Masao Hirotsu, Yosuke Amemiya, Kenji Nagakubo, Yuki Oyama, Toshio Iimuro, Yuji Hosoda, Kenji Kojima, Yuichiro Mochizuki, Hitoshi Nakagomi, Hiroshi Omata, Masao |
| description | Abstract
Background
The clonality of primary tumors is well elucidated. In contrast, the relation to metastatic foci is not well understood. We examined genomic clonality of primary tumors and metastatic foci.
Method
We studied 144 foci (34 primaries, 104 lymph nodes, 6 organ mets)
from 10 patients with gastrectomy (c-Stage 3). We also searched the expression of MMR proteins.
Results
In 10 primaries, mutations were found in all (range 3-12, mean 6). Of 104 lymph nodes, 64 lymph nodes carrying mutations identical to corresponding primaries in all. In addition, all the genomic profiles were replicated in corresponding lymph nodes and visceral mets. Among 3 cases with dMMR, all metastatic foci showed identical phenotypes.
Conclusion
Surprisingly, significantly mutated genes (SMGs) profiles in all lymph nodes and visceral mets were identical to the primaries. In addition, MMR/MSI status was also identical. These results indicated proper selection of chemo-, molecular-, and immune-oncology treatments could be effectively applied to the spreaded mets. Internoduler ""de novo"" heterogeneity was so scarce. Often claimed tumor heterogeneity could be due to inappropriate selection of drugs and due to their pressure. |
| doi_str_mv | 10.1093/annonc/mdz338.034 |
| format | Article |
| fullrecord | <record><control><sourceid>oup</sourceid><recordid>TN_cdi_oup_primary_10_1093_annonc_mdz338_034</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/annonc/mdz338.034</oup_id><sourcerecordid>10.1093/annonc/mdz338.034</sourcerecordid><originalsourceid>FETCH-oup_primary_10_1093_annonc_mdz338_0343</originalsourceid><addsrcrecordid>eNqVj01OhDAYhhujifhzAHffAYbhK4WRLs1EZ1wQE3XfVChaA21DywJXs_WankSa8QKu3uT9Sx5CbiiuKXKWSWOsabKh_WKsWiMrTkhCyw1PKyzoKUmQ5yy9LVlxTi68_0TEDc95Qg71E01pleZ3Rvaz1x5sB4MK0gcZdAOdbTRMXpt30AY-7OQV7JZwXLKygpd6B04a1ccZK-kKS_w5fJsA0rTQZq6unyFeTR7eZnjcb2Mxmm60QWnjr8hZJ3uvrv_0kqwe7l-3-9ROTrhRD3KcBUURKcWRUhwpxULJ_ln_BXhEWuE</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>MO1-18-2Analysis of metastatic foci using in house Gastric 58 SMG panel of 351,050 nt and d/pMMR status by IHC of MMR proteins</title><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Hada, Masao ; Hirotsu, Yosuke ; Amemiya, Kenji ; Nagakubo, Yuki ; Oyama, Toshio ; Iimuro, Yuji ; Hosoda, Kenji ; Kojima, Yuichiro ; Mochizuki, Hitoshi ; Nakagomi, Hiroshi ; Omata, Masao</creator><creatorcontrib>Hada, Masao ; Hirotsu, Yosuke ; Amemiya, Kenji ; Nagakubo, Yuki ; Oyama, Toshio ; Iimuro, Yuji ; Hosoda, Kenji ; Kojima, Yuichiro ; Mochizuki, Hitoshi ; Nakagomi, Hiroshi ; Omata, Masao</creatorcontrib><description>Abstract
Background
The clonality of primary tumors is well elucidated. In contrast, the relation to metastatic foci is not well understood. We examined genomic clonality of primary tumors and metastatic foci.
Method
We studied 144 foci (34 primaries, 104 lymph nodes, 6 organ mets)
from 10 patients with gastrectomy (c-Stage 3). We also searched the expression of MMR proteins.
Results
In 10 primaries, mutations were found in all (range 3-12, mean 6). Of 104 lymph nodes, 64 lymph nodes carrying mutations identical to corresponding primaries in all. In addition, all the genomic profiles were replicated in corresponding lymph nodes and visceral mets. Among 3 cases with dMMR, all metastatic foci showed identical phenotypes.
Conclusion
Surprisingly, significantly mutated genes (SMGs) profiles in all lymph nodes and visceral mets were identical to the primaries. In addition, MMR/MSI status was also identical. These results indicated proper selection of chemo-, molecular-, and immune-oncology treatments could be effectively applied to the spreaded mets. Internoduler ""de novo"" heterogeneity was so scarce. Often claimed tumor heterogeneity could be due to inappropriate selection of drugs and due to their pressure.</description><identifier>ISSN: 0923-7534</identifier><identifier>EISSN: 1569-8041</identifier><identifier>DOI: 10.1093/annonc/mdz338.034</identifier><language>eng</language><publisher>Oxford University Press</publisher><ispartof>Annals of oncology, 2019-10, Vol.30 (Supplement_6)</ispartof><rights>The Author(s) 2019. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved. For permissions, please email: journals.permissions@oup.com. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Hada, Masao</creatorcontrib><creatorcontrib>Hirotsu, Yosuke</creatorcontrib><creatorcontrib>Amemiya, Kenji</creatorcontrib><creatorcontrib>Nagakubo, Yuki</creatorcontrib><creatorcontrib>Oyama, Toshio</creatorcontrib><creatorcontrib>Iimuro, Yuji</creatorcontrib><creatorcontrib>Hosoda, Kenji</creatorcontrib><creatorcontrib>Kojima, Yuichiro</creatorcontrib><creatorcontrib>Mochizuki, Hitoshi</creatorcontrib><creatorcontrib>Nakagomi, Hiroshi</creatorcontrib><creatorcontrib>Omata, Masao</creatorcontrib><title>MO1-18-2Analysis of metastatic foci using in house Gastric 58 SMG panel of 351,050 nt and d/pMMR status by IHC of MMR proteins</title><title>Annals of oncology</title><description>Abstract
Background
The clonality of primary tumors is well elucidated. In contrast, the relation to metastatic foci is not well understood. We examined genomic clonality of primary tumors and metastatic foci.
Method
We studied 144 foci (34 primaries, 104 lymph nodes, 6 organ mets)
from 10 patients with gastrectomy (c-Stage 3). We also searched the expression of MMR proteins.
Results
In 10 primaries, mutations were found in all (range 3-12, mean 6). Of 104 lymph nodes, 64 lymph nodes carrying mutations identical to corresponding primaries in all. In addition, all the genomic profiles were replicated in corresponding lymph nodes and visceral mets. Among 3 cases with dMMR, all metastatic foci showed identical phenotypes.
Conclusion
Surprisingly, significantly mutated genes (SMGs) profiles in all lymph nodes and visceral mets were identical to the primaries. In addition, MMR/MSI status was also identical. These results indicated proper selection of chemo-, molecular-, and immune-oncology treatments could be effectively applied to the spreaded mets. Internoduler ""de novo"" heterogeneity was so scarce. Often claimed tumor heterogeneity could be due to inappropriate selection of drugs and due to their pressure.</description><issn>0923-7534</issn><issn>1569-8041</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqVj01OhDAYhhujifhzAHffAYbhK4WRLs1EZ1wQE3XfVChaA21DywJXs_WankSa8QKu3uT9Sx5CbiiuKXKWSWOsabKh_WKsWiMrTkhCyw1PKyzoKUmQ5yy9LVlxTi68_0TEDc95Qg71E01pleZ3Rvaz1x5sB4MK0gcZdAOdbTRMXpt30AY-7OQV7JZwXLKygpd6B04a1ccZK-kKS_w5fJsA0rTQZq6unyFeTR7eZnjcb2Mxmm60QWnjr8hZJ3uvrv_0kqwe7l-3-9ROTrhRD3KcBUURKcWRUhwpxULJ_ln_BXhEWuE</recordid><startdate>20191001</startdate><enddate>20191001</enddate><creator>Hada, Masao</creator><creator>Hirotsu, Yosuke</creator><creator>Amemiya, Kenji</creator><creator>Nagakubo, Yuki</creator><creator>Oyama, Toshio</creator><creator>Iimuro, Yuji</creator><creator>Hosoda, Kenji</creator><creator>Kojima, Yuichiro</creator><creator>Mochizuki, Hitoshi</creator><creator>Nakagomi, Hiroshi</creator><creator>Omata, Masao</creator><general>Oxford University Press</general><scope/></search><sort><creationdate>20191001</creationdate><title>MO1-18-2Analysis of metastatic foci using in house Gastric 58 SMG panel of 351,050 nt and d/pMMR status by IHC of MMR proteins</title><author>Hada, Masao ; Hirotsu, Yosuke ; Amemiya, Kenji ; Nagakubo, Yuki ; Oyama, Toshio ; Iimuro, Yuji ; Hosoda, Kenji ; Kojima, Yuichiro ; Mochizuki, Hitoshi ; Nakagomi, Hiroshi ; Omata, Masao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-oup_primary_10_1093_annonc_mdz338_0343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hada, Masao</creatorcontrib><creatorcontrib>Hirotsu, Yosuke</creatorcontrib><creatorcontrib>Amemiya, Kenji</creatorcontrib><creatorcontrib>Nagakubo, Yuki</creatorcontrib><creatorcontrib>Oyama, Toshio</creatorcontrib><creatorcontrib>Iimuro, Yuji</creatorcontrib><creatorcontrib>Hosoda, Kenji</creatorcontrib><creatorcontrib>Kojima, Yuichiro</creatorcontrib><creatorcontrib>Mochizuki, Hitoshi</creatorcontrib><creatorcontrib>Nakagomi, Hiroshi</creatorcontrib><creatorcontrib>Omata, Masao</creatorcontrib><jtitle>Annals of oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hada, Masao</au><au>Hirotsu, Yosuke</au><au>Amemiya, Kenji</au><au>Nagakubo, Yuki</au><au>Oyama, Toshio</au><au>Iimuro, Yuji</au><au>Hosoda, Kenji</au><au>Kojima, Yuichiro</au><au>Mochizuki, Hitoshi</au><au>Nakagomi, Hiroshi</au><au>Omata, Masao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MO1-18-2Analysis of metastatic foci using in house Gastric 58 SMG panel of 351,050 nt and d/pMMR status by IHC of MMR proteins</atitle><jtitle>Annals of oncology</jtitle><date>2019-10-01</date><risdate>2019</risdate><volume>30</volume><issue>Supplement_6</issue><issn>0923-7534</issn><eissn>1569-8041</eissn><abstract>Abstract
Background
The clonality of primary tumors is well elucidated. In contrast, the relation to metastatic foci is not well understood. We examined genomic clonality of primary tumors and metastatic foci.
Method
We studied 144 foci (34 primaries, 104 lymph nodes, 6 organ mets)
from 10 patients with gastrectomy (c-Stage 3). We also searched the expression of MMR proteins.
Results
In 10 primaries, mutations were found in all (range 3-12, mean 6). Of 104 lymph nodes, 64 lymph nodes carrying mutations identical to corresponding primaries in all. In addition, all the genomic profiles were replicated in corresponding lymph nodes and visceral mets. Among 3 cases with dMMR, all metastatic foci showed identical phenotypes.
Conclusion
Surprisingly, significantly mutated genes (SMGs) profiles in all lymph nodes and visceral mets were identical to the primaries. In addition, MMR/MSI status was also identical. These results indicated proper selection of chemo-, molecular-, and immune-oncology treatments could be effectively applied to the spreaded mets. Internoduler ""de novo"" heterogeneity was so scarce. Often claimed tumor heterogeneity could be due to inappropriate selection of drugs and due to their pressure.</abstract><pub>Oxford University Press</pub><doi>10.1093/annonc/mdz338.034</doi></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| title | MO1-18-2Analysis of metastatic foci using in house Gastric 58 SMG panel of 351,050 nt and d/pMMR status by IHC of MMR proteins |
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