Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria
Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is ins...
Gespeichert in:
| Veröffentlicht in: | Biochemistry (Easton) 2008-12, Vol.47 (49), p.12939-12941 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 12941 |
|---|---|
| container_issue | 49 |
| container_start_page | 12939 |
| container_title | Biochemistry (Easton) |
| container_volume | 47 |
| creator | Summerfield, Tina C. Toepel, Jörg Sherman, Louis A. |
| description | Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is inserted into functional PSII complexes. Two other cyanobacterial strains have psbA genes that were induced by low oxygen. In two of the three strains examined, psbA was part of an upregulated gene cluster including an alternative Rieske iron−sulfur protein. We conclude this cluster may represent an important adaptation to changing O2 levels that cyanobacteria experience. |
| doi_str_mv | 10.1021/bi8018916 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_959191</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>754540545</sourcerecordid><originalsourceid>FETCH-LOGICAL-a475t-7b08926fe3674f49c5fe59c93d12152f16fb28a68cfa72cee52a3b35b5372a3c3</originalsourceid><addsrcrecordid>eNp90ctu1DAUBmCrArXTlgUvgMICqi5S7CS242UVelNHLRIDCzaW4zkGl4w92I7avD1GGZUNYuOL_Ok_1jkIvSb4jOCKfOhti0krCNtDC0IrXDZC0BdogTFmZSUYPkCHMT7ka4N5s48OMhYtx3yBPi79Y3n_NH0HV9y49aiT9a7wprjzYaOGYSq6MG2T1cU29ufFFTiIhXVFNynne6UTBKuO0UujhgivdvsR-nJ5sequy-X91U13vixVw2kqeY9bUTEDNeONaYSmBqjQol6TKn_bEGb6qlWs1UbxSgPQStV9TXta83zS9RF6O-f6mKyM2ibQP7R3DnSSggoiSDYns9kG_2uEmOTGRg3DoBz4MUpOG9rgvGT5_r-SMZbzWp7h6Qx18DEGMHIb7EaFSRIs_wxAPg8g2ze70LHfwPqv3HU8g3IGNiZ4en5X4adkvOZUrj59lit6_fX27tut7LJ_N3ulo3zwY3C5w_8o_BtDcZl1</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>66691387</pqid></control><display><type>article</type><title>Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Summerfield, Tina C. ; Toepel, Jörg ; Sherman, Louis A.</creator><creatorcontrib>Summerfield, Tina C. ; Toepel, Jörg ; Sherman, Louis A. ; Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)</creatorcontrib><description>Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is inserted into functional PSII complexes. Two other cyanobacterial strains have psbA genes that were induced by low oxygen. In two of the three strains examined, psbA was part of an upregulated gene cluster including an alternative Rieske iron−sulfur protein. We conclude this cluster may represent an important adaptation to changing O2 levels that cyanobacteria experience.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi8018916</identifier><identifier>PMID: 18998707</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>BASIC BIOLOGICAL SCIENCES ; CYANOBACTERIA ; Cyanobacteria - genetics ; Cyanobacteria - metabolism ; Environmental Molecular Sciences Laboratory ; FUNCTIONALS ; Gene Expression Regulation, Bacterial ; GENES ; INDUCTION ; Microarray Analysis ; OXYGEN ; Photosystem II Protein Complex - biosynthesis ; Photosystem II Protein Complex - genetics ; PROTEINS ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; STRAINS ; Synechocystis ; Transcription, Genetic</subject><ispartof>Biochemistry (Easton), 2008-12, Vol.47 (49), p.12939-12941</ispartof><rights>Copyright © 2008 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a475t-7b08926fe3674f49c5fe59c93d12152f16fb28a68cfa72cee52a3b35b5372a3c3</citedby><cites>FETCH-LOGICAL-a475t-7b08926fe3674f49c5fe59c93d12152f16fb28a68cfa72cee52a3b35b5372a3c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi8018916$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi8018916$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,777,781,882,2752,27057,27905,27906,56719,56769</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18998707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/959191$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Summerfield, Tina C.</creatorcontrib><creatorcontrib>Toepel, Jörg</creatorcontrib><creatorcontrib>Sherman, Louis A.</creatorcontrib><creatorcontrib>Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)</creatorcontrib><title>Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is inserted into functional PSII complexes. Two other cyanobacterial strains have psbA genes that were induced by low oxygen. In two of the three strains examined, psbA was part of an upregulated gene cluster including an alternative Rieske iron−sulfur protein. We conclude this cluster may represent an important adaptation to changing O2 levels that cyanobacteria experience.</description><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>CYANOBACTERIA</subject><subject>Cyanobacteria - genetics</subject><subject>Cyanobacteria - metabolism</subject><subject>Environmental Molecular Sciences Laboratory</subject><subject>FUNCTIONALS</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>GENES</subject><subject>INDUCTION</subject><subject>Microarray Analysis</subject><subject>OXYGEN</subject><subject>Photosystem II Protein Complex - biosynthesis</subject><subject>Photosystem II Protein Complex - genetics</subject><subject>PROTEINS</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>STRAINS</subject><subject>Synechocystis</subject><subject>Transcription, Genetic</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90ctu1DAUBmCrArXTlgUvgMICqi5S7CS242UVelNHLRIDCzaW4zkGl4w92I7avD1GGZUNYuOL_Ok_1jkIvSb4jOCKfOhti0krCNtDC0IrXDZC0BdogTFmZSUYPkCHMT7ka4N5s48OMhYtx3yBPi79Y3n_NH0HV9y49aiT9a7wprjzYaOGYSq6MG2T1cU29ufFFTiIhXVFNynne6UTBKuO0UujhgivdvsR-nJ5sequy-X91U13vixVw2kqeY9bUTEDNeONaYSmBqjQol6TKn_bEGb6qlWs1UbxSgPQStV9TXta83zS9RF6O-f6mKyM2ibQP7R3DnSSggoiSDYns9kG_2uEmOTGRg3DoBz4MUpOG9rgvGT5_r-SMZbzWp7h6Qx18DEGMHIb7EaFSRIs_wxAPg8g2ze70LHfwPqv3HU8g3IGNiZ4en5X4adkvOZUrj59lit6_fX27tut7LJ_N3ulo3zwY3C5w_8o_BtDcZl1</recordid><startdate>20081209</startdate><enddate>20081209</enddate><creator>Summerfield, Tina C.</creator><creator>Toepel, Jörg</creator><creator>Sherman, Louis A.</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>OTOTI</scope></search><sort><creationdate>20081209</creationdate><title>Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria</title><author>Summerfield, Tina C. ; Toepel, Jörg ; Sherman, Louis A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a475t-7b08926fe3674f49c5fe59c93d12152f16fb28a68cfa72cee52a3b35b5372a3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>CYANOBACTERIA</topic><topic>Cyanobacteria - genetics</topic><topic>Cyanobacteria - metabolism</topic><topic>Environmental Molecular Sciences Laboratory</topic><topic>FUNCTIONALS</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>GENES</topic><topic>INDUCTION</topic><topic>Microarray Analysis</topic><topic>OXYGEN</topic><topic>Photosystem II Protein Complex - biosynthesis</topic><topic>Photosystem II Protein Complex - genetics</topic><topic>PROTEINS</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>STRAINS</topic><topic>Synechocystis</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Summerfield, Tina C.</creatorcontrib><creatorcontrib>Toepel, Jörg</creatorcontrib><creatorcontrib>Sherman, Louis A.</creatorcontrib><creatorcontrib>Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>OSTI.GOV</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Summerfield, Tina C.</au><au>Toepel, Jörg</au><au>Sherman, Louis A.</au><aucorp>Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>2008-12-09</date><risdate>2008</risdate><volume>47</volume><issue>49</issue><spage>12939</spage><epage>12941</epage><pages>12939-12941</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Microarray analysis indicated low-O2 conditions resulted in upregulation of psbA1, the normally low-abundance transcript that encodes the D1′ protein of photosystem II in Synechocystis sp. PCC 6803. Using a ΔpsbA2:ΔpsbA3 strain, we show the psbA1 transcript is translated and the resultant D1′ is inserted into functional PSII complexes. Two other cyanobacterial strains have psbA genes that were induced by low oxygen. In two of the three strains examined, psbA was part of an upregulated gene cluster including an alternative Rieske iron−sulfur protein. We conclude this cluster may represent an important adaptation to changing O2 levels that cyanobacteria experience.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>18998707</pmid><doi>10.1021/bi8018916</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-2960 |
| ispartof | Biochemistry (Easton), 2008-12, Vol.47 (49), p.12939-12941 |
| issn | 0006-2960 1520-4995 |
| language | eng |
| recordid | cdi_osti_scitechconnect_959191 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | BASIC BIOLOGICAL SCIENCES CYANOBACTERIA Cyanobacteria - genetics Cyanobacteria - metabolism Environmental Molecular Sciences Laboratory FUNCTIONALS Gene Expression Regulation, Bacterial GENES INDUCTION Microarray Analysis OXYGEN Photosystem II Protein Complex - biosynthesis Photosystem II Protein Complex - genetics PROTEINS RNA, Messenger - genetics RNA, Messenger - metabolism STRAINS Synechocystis Transcription, Genetic |
| title | Low-Oxygen Induction of Normally Cryptic psbA Genes in Cyanobacteria |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T23%3A24%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Low-Oxygen%20Induction%20of%20Normally%20Cryptic%20psbA%20Genes%20in%20Cyanobacteria&rft.jtitle=Biochemistry%20(Easton)&rft.au=Summerfield,%20Tina%20C.&rft.aucorp=Pacific%20Northwest%20National%20Lab.%20(PNNL),%20Richland,%20WA%20(United%20States).%20Environmental%20Molecular%20Sciences%20Lab.%20(EMSL)&rft.date=2008-12-09&rft.volume=47&rft.issue=49&rft.spage=12939&rft.epage=12941&rft.pages=12939-12941&rft.issn=0006-2960&rft.eissn=1520-4995&rft_id=info:doi/10.1021/bi8018916&rft_dat=%3Cproquest_osti_%3E754540545%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=66691387&rft_id=info:pmid/18998707&rfr_iscdi=true |