Structural Evidence for an Enolate Intermediate in GFP Fluorophore Biosynthesis

The Aequorea victoria green fluorescent protein (GFP) creates a fluorophore from its component amino acids Ser65, Tyr66, and Gly67 through a remarkable post-translational modification, involving spontaneous peptide backbone cyclization, dehydration, and oxidation reactions. Here we test and extend t...

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Veröffentlicht in:	Journal of the American Chemical Society 2006-03, Vol.128 (10), p.3166-3168
Hauptverfasser:	Barondeau, David P, Tainer, John A, Getzoff, Elizabeth D
Format:	Artikel
Sprache:	eng
Schlagworte:	BASIC BIOLOGICAL SCIENCES Biological and medical sciences BIOSYNTHESIS Crystalline structure Crystallography, X-Ray FLUORINE COMPOUNDS Fundamental and applied biological sciences. Psychology Glycine - metabolism Green Fluorescent Proteins - biosynthesis Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism Kinetics Models, Molecular Molecular biophysics MORPHOLOGY Other,OTHER Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary REACTION INTERMEDIATES Serine - metabolism Structure in molecular biology Tyrosine - metabolism
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