DNA Capillary Electrophoresis in Entangled Dynamic Polymers of Surfactant Molecules
Aqueous solutions of monomeric nonionic surfactants, n-alkyl polyoxyethylene ethers (C16E6, C16E8, C14E6), can be used as sieving matrixes for the separation of DNA fragments by capillary electrophoresis. Unlike ordinary polymer solutions, these surfactant solutions behave as dynamic polymers. By co...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2001-04, Vol.73 (8), p.1776-1783 |
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| creator | Wei Yeung, Edward S |
| description | Aqueous solutions of monomeric nonionic surfactants, n-alkyl polyoxyethylene ethers (C16E6, C16E8, C14E6), can be used as sieving matrixes for the separation of DNA fragments by capillary electrophoresis. Unlike ordinary polymer solutions, these surfactant solutions behave as dynamic polymers. By combining the “reversible gel” theory of DNA electrophoresis and the static and dynamic properties of wormlike surfactant micelles, a model is developed for describing the migration behavior of DNA molecules in these solutions. According to the model, the separation limit can be extended at low surfactant concentrations. Surfactant solutions as a separation medium provide many advantages over ordinary polymers, such as ease of preparation, solution homogeneity, stable structure, low viscosity, and self-coating property for reducing electroosmotic flow. More importantly, the properties of wormlike micelles (micelle size, entanglement concentration) can be adjusted by simply changing the monomer concentration, denaturant, and temperature to allow the separation of different size ranges of DNA fragments. Fast separation is achieved for DNA fragments ranging from 10 bp to 5 kb by using bare fused-silica columns. DNA sequencing fragments of BigDye G-labeled M13 up to 600 bases were separated within 60 min. |
| doi_str_mv | 10.1021/ac0012997 |
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Unlike ordinary polymer solutions, these surfactant solutions behave as dynamic polymers. By combining the “reversible gel” theory of DNA electrophoresis and the static and dynamic properties of wormlike surfactant micelles, a model is developed for describing the migration behavior of DNA molecules in these solutions. According to the model, the separation limit can be extended at low surfactant concentrations. Surfactant solutions as a separation medium provide many advantages over ordinary polymers, such as ease of preparation, solution homogeneity, stable structure, low viscosity, and self-coating property for reducing electroosmotic flow. More importantly, the properties of wormlike micelles (micelle size, entanglement concentration) can be adjusted by simply changing the monomer concentration, denaturant, and temperature to allow the separation of different size ranges of DNA fragments. Fast separation is achieved for DNA fragments ranging from 10 bp to 5 kb by using bare fused-silica columns. DNA sequencing fragments of BigDye G-labeled M13 up to 600 bases were separated within 60 min.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac0012997</identifier><identifier>PMID: 11338591</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; Blood vessels ; capillary electrophoresis ; Deoxyribonucleic acid ; Diverse techniques ; DNA ; DNA - analysis ; Dna, deoxyribonucleoproteins ; ELECTROPHORESIS ; Electrophoresis, Capillary ; Fundamental and applied biological sciences. Psychology ; Micelles ; Molecular and cellular biology ; Molecules ; Nucleic acids ; POLYMERS ; Sequence Analysis, DNA ; Surface-Active Agents - chemistry ; SURFACTANTS</subject><ispartof>Analytical chemistry (Washington), 2001-04, Vol.73 (8), p.1776-1783</ispartof><rights>Copyright © 2001 American Chemical Society</rights><rights>2001 INIST-CNRS</rights><rights>Copyright American Chemical Society Apr 15, 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a528t-a2b053c4810887b0505ef62a1eaf0b3de6232d90093c325237dd227af831519c3</citedby><cites>FETCH-LOGICAL-a528t-a2b053c4810887b0505ef62a1eaf0b3de6232d90093c325237dd227af831519c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac0012997$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac0012997$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,315,781,785,886,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1005731$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11338591$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/791714$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wei</creatorcontrib><creatorcontrib>Yeung, Edward S</creatorcontrib><creatorcontrib>Ames Laboratory (AMES), Ames, IA</creatorcontrib><title>DNA Capillary Electrophoresis in Entangled Dynamic Polymers of Surfactant Molecules</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Aqueous solutions of monomeric nonionic surfactants, n-alkyl polyoxyethylene ethers (C16E6, C16E8, C14E6), can be used as sieving matrixes for the separation of DNA fragments by capillary electrophoresis. Unlike ordinary polymer solutions, these surfactant solutions behave as dynamic polymers. By combining the “reversible gel” theory of DNA electrophoresis and the static and dynamic properties of wormlike surfactant micelles, a model is developed for describing the migration behavior of DNA molecules in these solutions. According to the model, the separation limit can be extended at low surfactant concentrations. Surfactant solutions as a separation medium provide many advantages over ordinary polymers, such as ease of preparation, solution homogeneity, stable structure, low viscosity, and self-coating property for reducing electroosmotic flow. More importantly, the properties of wormlike micelles (micelle size, entanglement concentration) can be adjusted by simply changing the monomer concentration, denaturant, and temperature to allow the separation of different size ranges of DNA fragments. Fast separation is achieved for DNA fragments ranging from 10 bp to 5 kb by using bare fused-silica columns. DNA sequencing fragments of BigDye G-labeled M13 up to 600 bases were separated within 60 min.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>Blood vessels</subject><subject>capillary electrophoresis</subject><subject>Deoxyribonucleic acid</subject><subject>Diverse techniques</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>ELECTROPHORESIS</subject><subject>Electrophoresis, Capillary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Micelles</subject><subject>Molecular and cellular biology</subject><subject>Molecules</subject><subject>Nucleic acids</subject><subject>POLYMERS</subject><subject>Sequence Analysis, DNA</subject><subject>Surface-Active Agents - chemistry</subject><subject>SURFACTANTS</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c-P1CAUB3BiNO64evAfMHWjJh6q78FQ6HEzO_5IVh0z65kwlLqsLYzQJs5_L00nu0YPhgMkfHjw5RHyFOENAsW32gAgrWtxjyyQUygrKel9sgAAVlIBcEIepXSTEQJWD8kJImOS17gg24vP58VK713X6Xgo1p01Qwz76xBtcqlwvlj7QfvvnW2Ki4PXvTPFJnSH3sZUhLbYjrHVJouh-BTy4bGz6TF50Oou2SfH-ZR8e7e-Wn0oL7-8_7g6vyw1p3IoNd0BZ2YpEaQUeQ3cthXVaHULO9bYijLa1AA1M4xyykTTUCp0KxlyrA07Jc_nuiENTiXjBmuuTfA-Z1CiRoHLbF7NZh_Dz9GmQfUuGZvTehvGpATIaYj_QhSyZrCc4Nlf8CaM0eegimYkJUeZ0esZmRhSirZV--j6_MMKQU09U7c9y_bZseC4621zJ49NyuDFEehkdNdG7Y1Lf1QELtjEypm5NNhft9s6_lCVYIKrq81WfRVUbIAv1XTvy9lrk-4y_Pu-31aLtUo</recordid><startdate>20010415</startdate><enddate>20010415</enddate><creator>Wei</creator><creator>Yeung, Edward S</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>20010415</creationdate><title>DNA Capillary Electrophoresis in Entangled Dynamic Polymers of Surfactant Molecules</title><author>Wei ; Yeung, Edward S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a528t-a2b053c4810887b0505ef62a1eaf0b3de6232d90093c325237dd227af831519c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>Blood vessels</topic><topic>capillary electrophoresis</topic><topic>Deoxyribonucleic acid</topic><topic>Diverse techniques</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>ELECTROPHORESIS</topic><topic>Electrophoresis, Capillary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Micelles</topic><topic>Molecular and cellular biology</topic><topic>Molecules</topic><topic>Nucleic acids</topic><topic>POLYMERS</topic><topic>Sequence Analysis, DNA</topic><topic>Surface-Active Agents - chemistry</topic><topic>SURFACTANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei</creatorcontrib><creatorcontrib>Yeung, Edward S</creatorcontrib><creatorcontrib>Ames Laboratory (AMES), Ames, IA</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei</au><au>Yeung, Edward S</au><aucorp>Ames Laboratory (AMES), Ames, IA</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Capillary Electrophoresis in Entangled Dynamic Polymers of Surfactant Molecules</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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Surfactant solutions as a separation medium provide many advantages over ordinary polymers, such as ease of preparation, solution homogeneity, stable structure, low viscosity, and self-coating property for reducing electroosmotic flow. More importantly, the properties of wormlike micelles (micelle size, entanglement concentration) can be adjusted by simply changing the monomer concentration, denaturant, and temperature to allow the separation of different size ranges of DNA fragments. Fast separation is achieved for DNA fragments ranging from 10 bp to 5 kb by using bare fused-silica columns. DNA sequencing fragments of BigDye G-labeled M13 up to 600 bases were separated within 60 min.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>11338591</pmid><doi>10.1021/ac0012997</doi><tpages>8</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2001-04, Vol.73 (8), p.1776-1783 |
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| source | MEDLINE; ACS Publications |
| subjects | Analysis Analytical, structural and metabolic biochemistry BASIC BIOLOGICAL SCIENCES Biological and medical sciences Blood vessels capillary electrophoresis Deoxyribonucleic acid Diverse techniques DNA DNA - analysis Dna, deoxyribonucleoproteins ELECTROPHORESIS Electrophoresis, Capillary Fundamental and applied biological sciences. Psychology Micelles Molecular and cellular biology Molecules Nucleic acids POLYMERS Sequence Analysis, DNA Surface-Active Agents - chemistry SURFACTANTS |
| title | DNA Capillary Electrophoresis in Entangled Dynamic Polymers of Surfactant Molecules |
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