Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells
We have recently described a signal transducing molecule, 2B4, expressed on all NK and T cells that mediate non-MHC-restricted killing. The gene encoding this molecule was cloned and its nucleotide sequence determined. The encoded protein of 398 amino acids has a leader peptide of 18 amino acids and...
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| Veröffentlicht in: | The Journal of immunology (1950) 1993-11, Vol.151 (10), p.5328-5337 |
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| creator | Mathew, PA Garni-Wagner, BA Land, K Takashima, A Stoneman, E Bennett, M Kumar, V |
| description | We have recently described a signal transducing molecule, 2B4, expressed on all NK and T cells that mediate non-MHC-restricted killing. The gene encoding this molecule was cloned and its nucleotide sequence determined. The encoded protein of 398 amino acids has a leader peptide of 18 amino acids and a transmembrane region of 24 amino acids. The predicted protein has eight N-linked glycosylation sites, suggesting that it is highly glycosylated. Comparison of 2B4 with sequences in the databanks indicates that 2B4 is a member of Ig supergene family, and it shows homology to murine and rat CD48 and human LFA-3. Northern blot analysis has shown at least three transcripts for 2B4 in adherent lymphokine-activated killer cells of several mouse strains and TCR-gamma/delta dendritic epidermal T cell lines but not in allospecific T cell clones. These three mRNA are the products of differential splicing of heterogeneous nuclear RNA. Southern blot analysis of genomic DNA from several mouse strains revealed that 2B4 belongs to a family of closely related genes. The 2B4 gene has been mapped to mouse chromosome 1 by analysis of 2B4 expression in recombinant inbred mouse strains. |
| doi_str_mv | 10.4049/jimmunol.151.10.5328 |
| format | Article |
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The gene encoding this molecule was cloned and its nucleotide sequence determined. The encoded protein of 398 amino acids has a leader peptide of 18 amino acids and a transmembrane region of 24 amino acids. The predicted protein has eight N-linked glycosylation sites, suggesting that it is highly glycosylated. Comparison of 2B4 with sequences in the databanks indicates that 2B4 is a member of Ig supergene family, and it shows homology to murine and rat CD48 and human LFA-3. Northern blot analysis has shown at least three transcripts for 2B4 in adherent lymphokine-activated killer cells of several mouse strains and TCR-gamma/delta dendritic epidermal T cell lines but not in allospecific T cell clones. These three mRNA are the products of differential splicing of heterogeneous nuclear RNA. Southern blot analysis of genomic DNA from several mouse strains revealed that 2B4 belongs to a family of closely related genes. The 2B4 gene has been mapped to mouse chromosome 1 by analysis of 2B4 expression in recombinant inbred mouse strains.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.151.10.5328</identifier><identifier>PMID: 8228228</identifier><identifier>CODEN: JOIMA3</identifier><language>eng</language><publisher>Bethesda, MD: Am Assoc Immnol</publisher><subject>AMINO ACID SEQUENCE ; ANIMAL CELLS ; Animals ; Antigens, CD ; Base Sequence ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; BIOLOGICAL MATERIALS ; BLOOD ; BLOOD CELLS ; BODY FLUIDS ; CELL KILLING ; Cells, Cultured ; Chromosome Mapping ; CLONING ; Cloning, Molecular ; CONNECTIVE TISSUE CELLS ; Cytotoxicity, Immunologic ; DNA HYBRIDIZATION ; DNA SEQUENCING ; DNA, Complementary - chemistry ; DNA, Complementary - isolation & purification ; DNA-CLONING ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; GENES ; GLYCOPROTEINS ; Humans ; HYBRIDIZATION ; Immunobiology ; Killer Cells, Natural - immunology ; LEUKOCYTES ; Lymphocyte Activation ; LYMPHOCYTES ; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation ; MATERIALS ; Membrane Glycoproteins ; Mice ; Mice, Inbred Strains ; Molecular Sequence Data ; MOLECULAR STRUCTURE ; NATURAL KILLER CELLS ; ORGANIC COMPOUNDS ; PROTEINS ; Proteins - chemistry ; Proteins - genetics ; Receptors, Immunologic ; RNA, Messenger - analysis ; Signal Transduction ; Signaling Lymphocytic Activation Molecule Family ; SOMATIC CELLS ; STRUCTURAL CHEMICAL ANALYSIS 550400 -- Genetics ; T-Lymphocytes - immunology</subject><ispartof>The Journal of immunology (1950), 1993-11, Vol.151 (10), p.5328-5337</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-c6ff38d2c7028b4fa602927387a94c7d3210e05cf49866a669b4d1790a6fc23f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3910559$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8228228$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7200950$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Mathew, PA</creatorcontrib><creatorcontrib>Garni-Wagner, BA</creatorcontrib><creatorcontrib>Land, K</creatorcontrib><creatorcontrib>Takashima, A</creatorcontrib><creatorcontrib>Stoneman, E</creatorcontrib><creatorcontrib>Bennett, M</creatorcontrib><creatorcontrib>Kumar, V</creatorcontrib><title>Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>We have recently described a signal transducing molecule, 2B4, expressed on all NK and T cells that mediate non-MHC-restricted killing. The gene encoding this molecule was cloned and its nucleotide sequence determined. The encoded protein of 398 amino acids has a leader peptide of 18 amino acids and a transmembrane region of 24 amino acids. The predicted protein has eight N-linked glycosylation sites, suggesting that it is highly glycosylated. Comparison of 2B4 with sequences in the databanks indicates that 2B4 is a member of Ig supergene family, and it shows homology to murine and rat CD48 and human LFA-3. Northern blot analysis has shown at least three transcripts for 2B4 in adherent lymphokine-activated killer cells of several mouse strains and TCR-gamma/delta dendritic epidermal T cell lines but not in allospecific T cell clones. These three mRNA are the products of differential splicing of heterogeneous nuclear RNA. Southern blot analysis of genomic DNA from several mouse strains revealed that 2B4 belongs to a family of closely related genes. The 2B4 gene has been mapped to mouse chromosome 1 by analysis of 2B4 expression in recombinant inbred mouse strains.</description><subject>AMINO ACID SEQUENCE</subject><subject>ANIMAL CELLS</subject><subject>Animals</subject><subject>Antigens, CD</subject><subject>Base Sequence</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BLOOD</subject><subject>BLOOD CELLS</subject><subject>BODY FLUIDS</subject><subject>CELL KILLING</subject><subject>Cells, Cultured</subject><subject>Chromosome Mapping</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>Cytotoxicity, Immunologic</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA SEQUENCING</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - isolation & purification</subject><subject>DNA-CLONING</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>GENES</subject><subject>GLYCOPROTEINS</subject><subject>Humans</subject><subject>HYBRIDIZATION</subject><subject>Immunobiology</subject><subject>Killer Cells, Natural - immunology</subject><subject>LEUKOCYTES</subject><subject>Lymphocyte Activation</subject><subject>LYMPHOCYTES</subject><subject>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</subject><subject>MATERIALS</subject><subject>Membrane Glycoproteins</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular Sequence Data</subject><subject>MOLECULAR STRUCTURE</subject><subject>NATURAL KILLER CELLS</subject><subject>ORGANIC COMPOUNDS</subject><subject>PROTEINS</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>Receptors, Immunologic</subject><subject>RNA, Messenger - analysis</subject><subject>Signal Transduction</subject><subject>Signaling Lymphocytic Activation Molecule Family</subject><subject>SOMATIC CELLS</subject><subject>STRUCTURAL CHEMICAL ANALYSIS 550400 -- Genetics</subject><subject>T-Lymphocytes - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd2O1CAUxxujWWdX30ATYszGm44HSqG91Im6Jmu8Wa8JQ-mUlcIK1GZ9HJ9UOh033pmQwDnnd774F8ULDFsKtH17a8Zxct5ucY232VlXpHlUbHBdQ8kYsMfFBoCQEnPGnxbnMd4CAANCz4qzhpDlbIrfO-udcQckXYfUIINUSQfzSybjHfI9SoNG5D1FB-000k757kij0VutJquRjNErI5Pu0GzSgJx35ZerXRl0TMGoxf_dWLtkjbpbwf09yn3Mz6PhZJqCtEdKB6S0tfE4zs36flY86aWN-vnpvii-ffxws7sqr79--rx7d10qSkgqFev7qumI4kCaPe1lXrUlvGq4bKniXUUwaKhVT9uGMclYu6cd5i1I1itS9dVF8Wqt62MyIiqTtBqUd06rJDgBaGvI0OUK3QX_Y8oritHEZUzptJ-i4AxqCg39L4gZpzWmPIN0BVXwMQbdi7tgRhnuBQaxCC3-Ci2y0ItzETqnvTzVn_b5Xx-STsrm-OtTXEYlbR-kUyY-YFWLoa7bjL1ZscEchtkELeIorc1FsZjn-d-OfwCjwMHQ</recordid><startdate>19931115</startdate><enddate>19931115</enddate><creator>Mathew, PA</creator><creator>Garni-Wagner, BA</creator><creator>Land, K</creator><creator>Takashima, A</creator><creator>Stoneman, E</creator><creator>Bennett, M</creator><creator>Kumar, V</creator><general>Am Assoc Immnol</general><general>American Association of Immunologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19931115</creationdate><title>Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells</title><author>Mathew, PA ; Garni-Wagner, BA ; Land, K ; Takashima, A ; Stoneman, E ; Bennett, M ; Kumar, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-c6ff38d2c7028b4fa602927387a94c7d3210e05cf49866a669b4d1790a6fc23f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>AMINO ACID SEQUENCE</topic><topic>ANIMAL CELLS</topic><topic>Animals</topic><topic>Antigens, CD</topic><topic>Base Sequence</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BLOOD</topic><topic>BLOOD CELLS</topic><topic>BODY FLUIDS</topic><topic>CELL KILLING</topic><topic>Cells, Cultured</topic><topic>Chromosome Mapping</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>Cytotoxicity, Immunologic</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA SEQUENCING</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - isolation & purification</topic><topic>DNA-CLONING</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>GENES</topic><topic>GLYCOPROTEINS</topic><topic>Humans</topic><topic>HYBRIDIZATION</topic><topic>Immunobiology</topic><topic>Killer Cells, Natural - immunology</topic><topic>LEUKOCYTES</topic><topic>Lymphocyte Activation</topic><topic>LYMPHOCYTES</topic><topic>Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation</topic><topic>MATERIALS</topic><topic>Membrane Glycoproteins</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular Sequence Data</topic><topic>MOLECULAR STRUCTURE</topic><topic>NATURAL KILLER CELLS</topic><topic>ORGANIC COMPOUNDS</topic><topic>PROTEINS</topic><topic>Proteins - chemistry</topic><topic>Proteins - genetics</topic><topic>Receptors, Immunologic</topic><topic>RNA, Messenger - analysis</topic><topic>Signal Transduction</topic><topic>Signaling Lymphocytic Activation Molecule Family</topic><topic>SOMATIC CELLS</topic><topic>STRUCTURAL CHEMICAL ANALYSIS 550400 -- Genetics</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mathew, PA</creatorcontrib><creatorcontrib>Garni-Wagner, BA</creatorcontrib><creatorcontrib>Land, K</creatorcontrib><creatorcontrib>Takashima, A</creatorcontrib><creatorcontrib>Stoneman, E</creatorcontrib><creatorcontrib>Bennett, M</creatorcontrib><creatorcontrib>Kumar, V</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mathew, PA</au><au>Garni-Wagner, BA</au><au>Land, K</au><au>Takashima, A</au><au>Stoneman, E</au><au>Bennett, M</au><au>Kumar, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1993-11-15</date><risdate>1993</risdate><volume>151</volume><issue>10</issue><spage>5328</spage><epage>5337</epage><pages>5328-5337</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><coden>JOIMA3</coden><abstract>We have recently described a signal transducing molecule, 2B4, expressed on all NK and T cells that mediate non-MHC-restricted killing. The gene encoding this molecule was cloned and its nucleotide sequence determined. The encoded protein of 398 amino acids has a leader peptide of 18 amino acids and a transmembrane region of 24 amino acids. The predicted protein has eight N-linked glycosylation sites, suggesting that it is highly glycosylated. Comparison of 2B4 with sequences in the databanks indicates that 2B4 is a member of Ig supergene family, and it shows homology to murine and rat CD48 and human LFA-3. Northern blot analysis has shown at least three transcripts for 2B4 in adherent lymphokine-activated killer cells of several mouse strains and TCR-gamma/delta dendritic epidermal T cell lines but not in allospecific T cell clones. These three mRNA are the products of differential splicing of heterogeneous nuclear RNA. Southern blot analysis of genomic DNA from several mouse strains revealed that 2B4 belongs to a family of closely related genes. The 2B4 gene has been mapped to mouse chromosome 1 by analysis of 2B4 expression in recombinant inbred mouse strains.</abstract><cop>Bethesda, MD</cop><pub>Am Assoc Immnol</pub><pmid>8228228</pmid><doi>10.4049/jimmunol.151.10.5328</doi><tpages>10</tpages></addata></record> |
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| ispartof | The Journal of immunology (1950), 1993-11, Vol.151 (10), p.5328-5337 |
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| language | eng |
| recordid | cdi_osti_scitechconnect_7200950 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | AMINO ACID SEQUENCE ANIMAL CELLS Animals Antigens, CD Base Sequence BASIC BIOLOGICAL SCIENCES Biological and medical sciences BIOLOGICAL MATERIALS BLOOD BLOOD CELLS BODY FLUIDS CELL KILLING Cells, Cultured Chromosome Mapping CLONING Cloning, Molecular CONNECTIVE TISSUE CELLS Cytotoxicity, Immunologic DNA HYBRIDIZATION DNA SEQUENCING DNA, Complementary - chemistry DNA, Complementary - isolation & purification DNA-CLONING Fundamental and applied biological sciences. Psychology Fundamental immunology GENES GLYCOPROTEINS Humans HYBRIDIZATION Immunobiology Killer Cells, Natural - immunology LEUKOCYTES Lymphocyte Activation LYMPHOCYTES Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation MATERIALS Membrane Glycoproteins Mice Mice, Inbred Strains Molecular Sequence Data MOLECULAR STRUCTURE NATURAL KILLER CELLS ORGANIC COMPOUNDS PROTEINS Proteins - chemistry Proteins - genetics Receptors, Immunologic RNA, Messenger - analysis Signal Transduction Signaling Lymphocytic Activation Molecule Family SOMATIC CELLS STRUCTURAL CHEMICAL ANALYSIS 550400 -- Genetics T-Lymphocytes - immunology |
| title | Cloning and characterization of the 2B4 gene encoding a molecule associated with non-MHC-restricted killing mediated by activated natural killer cells and T cells |
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