Ascorbic acid metabolism in protection against free radicals: A radiation model
The role of ascorbic acid in scavenging free radicals was evaluated in a model of mammalian colonic epithelium homogenized in physiologic buffer and exposed to ionizing radiation. Ascorbic acid interacts with hydroxyl free radicals, resulting in production of the ascorbate free radical (AḞR). Coloni...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1990-06, Vol.169 (2), p.430-436 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 436 |
|---|---|
| container_issue | 2 |
| container_start_page | 430 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 169 |
| creator | Rose, Richard C. |
| description | The role of ascorbic acid in scavenging free radicals was evaluated in a model of mammalian colonic epithelium homogenized in physiologic buffer and exposed to ionizing radiation. Ascorbic acid interacts with hydroxyl free radicals, resulting in production of the ascorbate free radical (AḞR). Colonic mucosa contains a soluble factor that is heat sensitive, PCA precipitable and is contained within 1,000 MW dialysis tubing; it uses GSH and cysteine to reduce AḞR. The factor from rat colon is fractionated between 55 and 70% saturation with solid (NH
4)
2SO
4; a 3–4 fold increase in enzyme activity was achieved. We suggest that the factor is a cytosolic enzyme appropriately referred to as soluble AḞR-reductase. This information provides insight into the mechanism by which ascorbic acid protects against damage by hydroxyl free radicals. |
| doi_str_mv | 10.1016/0006-291X(90)90349-R |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_7062359</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0006291X9090349R</els_id><sourcerecordid>79847999</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-a56a910d034428de498d67d04cf4f8291d8298bb44bf0a91a96de8e06405ce3a3</originalsourceid><addsrcrecordid>eNp9kFFL3TAUx4NsuDvnN3BYhA330O2kTdPGh8FF5hwIgpvgWzhNTjWjbTTpFfbtl95e3NteksD5nZP_-TF2xOEzBy6_AIDMC8XvThV8UlAKld_ssRUHBXnBQbxiqxfkDXsb428AzoVU-2y_4LKQslqx63U0PrTOZGiczQaasPW9i0Pmxuwx-InM5PyY4T26MU5ZF4iygNYZ7ONZtt6-cYsM3lL_jr3uUoUOd_cBu7349uv8Mr-6_v7jfH2VmwrElGMlUXGwKbUoGktCNVbWFoTpRNekxDYdTdsK0XaQSFTSUkMgBVSGSiwP2Mky18fJ6WhcCvpg_DimvLoGWZSVStDHBUqLPG0oTnpw0VDf40h-E3WtGlErNYNiAU3wMQbq9GNwA4Y_moOeZevZpJ5NagV6K1vfpLb3u_mbdiD70rSzm-ofdnWMyVcXcDQu_putRANVM3PHC9eh13gfEnP7swBeQlGnj1WZiK8LQcnps6Mwr0yjIevCvLH17v9R_wKkXqOL</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79847999</pqid></control><display><type>article</type><title>Ascorbic acid metabolism in protection against free radicals: A radiation model</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Rose, Richard C.</creator><creatorcontrib>Rose, Richard C.</creatorcontrib><description>The role of ascorbic acid in scavenging free radicals was evaluated in a model of mammalian colonic epithelium homogenized in physiologic buffer and exposed to ionizing radiation. Ascorbic acid interacts with hydroxyl free radicals, resulting in production of the ascorbate free radical (AḞR). Colonic mucosa contains a soluble factor that is heat sensitive, PCA precipitable and is contained within 1,000 MW dialysis tubing; it uses GSH and cysteine to reduce AḞR. The factor from rat colon is fractionated between 55 and 70% saturation with solid (NH
4)
2SO
4; a 3–4 fold increase in enzyme activity was achieved. We suggest that the factor is a cytosolic enzyme appropriately referred to as soluble AḞR-reductase. This information provides insight into the mechanism by which ascorbic acid protects against damage by hydroxyl free radicals.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(90)90349-R</identifier><identifier>PMID: 2162665</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture ; AMINO ACIDS ; ANIMAL TISSUES ; ANIMALS ; ASCORBIC ACID ; Ascorbic Acid - metabolism ; Ascorbic Acid - radiation effects ; Biological and medical sciences ; BIOSYNTHESIS ; BODY ; CARBOXYLIC ACIDS ; Cell physiology ; CHEMICAL REACTIONS ; COENZYMES ; colon ; Colon - metabolism ; CYSTEINE ; Cytosol - enzymology ; Dehydroascorbic Acid - metabolism ; DIGESTIVE SYSTEM ; Effects of physical and chemical agents ; electromagnetic radiation ; ENZYME ACTIVITY ; ENZYMES ; EPITHELIUM ; Free Radicals ; Fundamental and applied biological sciences. Psychology ; GASTROINTESTINAL TRACT ; Humans ; Hydroxides ; Hydroxyl Radical ; Intestinal Mucosa - metabolism ; INTESTINES ; IONIZING RADIATIONS ; Kinetics ; LARGE INTESTINE ; MAMMALS ; MAN ; MEMBRANES ; METABOLISM ; Molecular and cellular biology ; MUCOUS MEMBRANES ; NADH, NADPH Oxidoreductases - metabolism ; NADH2 ; NUCLEOTIDES ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; ORGANIC SULFUR COMPOUNDS ; ORGANS ; Oxidation-Reduction ; OXIDOREDUCTASES ; PRIMATES ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; RADICALS ; RADIOSENSITIVITY EFFECTS ; RATS ; Rats, Inbred Strains ; REDOX REACTIONS ; RODENTS ; SYNTHESIS ; THIOLS ; thrombin ; TISSUES ; VERTEBRATES ; VITAMINS</subject><ispartof>Biochemical and biophysical research communications, 1990-06, Vol.169 (2), p.430-436</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-a56a910d034428de498d67d04cf4f8291d8298bb44bf0a91a96de8e06405ce3a3</citedby><cites>FETCH-LOGICAL-c504t-a56a910d034428de498d67d04cf4f8291d8298bb44bf0a91a96de8e06405ce3a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0006-291X(90)90349-R$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19480585$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2162665$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7062359$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Rose, Richard C.</creatorcontrib><title>Ascorbic acid metabolism in protection against free radicals: A radiation model</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The role of ascorbic acid in scavenging free radicals was evaluated in a model of mammalian colonic epithelium homogenized in physiologic buffer and exposed to ionizing radiation. Ascorbic acid interacts with hydroxyl free radicals, resulting in production of the ascorbate free radical (AḞR). Colonic mucosa contains a soluble factor that is heat sensitive, PCA precipitable and is contained within 1,000 MW dialysis tubing; it uses GSH and cysteine to reduce AḞR. The factor from rat colon is fractionated between 55 and 70% saturation with solid (NH
4)
2SO
4; a 3–4 fold increase in enzyme activity was achieved. We suggest that the factor is a cytosolic enzyme appropriately referred to as soluble AḞR-reductase. This information provides insight into the mechanism by which ascorbic acid protects against damage by hydroxyl free radicals.</description><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</subject><subject>AMINO ACIDS</subject><subject>ANIMAL TISSUES</subject><subject>ANIMALS</subject><subject>ASCORBIC ACID</subject><subject>Ascorbic Acid - metabolism</subject><subject>Ascorbic Acid - radiation effects</subject><subject>Biological and medical sciences</subject><subject>BIOSYNTHESIS</subject><subject>BODY</subject><subject>CARBOXYLIC ACIDS</subject><subject>Cell physiology</subject><subject>CHEMICAL REACTIONS</subject><subject>COENZYMES</subject><subject>colon</subject><subject>Colon - metabolism</subject><subject>CYSTEINE</subject><subject>Cytosol - enzymology</subject><subject>Dehydroascorbic Acid - metabolism</subject><subject>DIGESTIVE SYSTEM</subject><subject>Effects of physical and chemical agents</subject><subject>electromagnetic radiation</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>EPITHELIUM</subject><subject>Free Radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GASTROINTESTINAL TRACT</subject><subject>Humans</subject><subject>Hydroxides</subject><subject>Hydroxyl Radical</subject><subject>Intestinal Mucosa - metabolism</subject><subject>INTESTINES</subject><subject>IONIZING RADIATIONS</subject><subject>Kinetics</subject><subject>LARGE INTESTINE</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>MEMBRANES</subject><subject>METABOLISM</subject><subject>Molecular and cellular biology</subject><subject>MUCOUS MEMBRANES</subject><subject>NADH, NADPH Oxidoreductases - metabolism</subject><subject>NADH2</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC SULFUR COMPOUNDS</subject><subject>ORGANS</subject><subject>Oxidation-Reduction</subject><subject>OXIDOREDUCTASES</subject><subject>PRIMATES</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>RADICALS</subject><subject>RADIOSENSITIVITY EFFECTS</subject><subject>RATS</subject><subject>Rats, Inbred Strains</subject><subject>REDOX REACTIONS</subject><subject>RODENTS</subject><subject>SYNTHESIS</subject><subject>THIOLS</subject><subject>thrombin</subject><subject>TISSUES</subject><subject>VERTEBRATES</subject><subject>VITAMINS</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFFL3TAUx4NsuDvnN3BYhA330O2kTdPGh8FF5hwIgpvgWzhNTjWjbTTpFfbtl95e3NteksD5nZP_-TF2xOEzBy6_AIDMC8XvThV8UlAKld_ssRUHBXnBQbxiqxfkDXsb428AzoVU-2y_4LKQslqx63U0PrTOZGiczQaasPW9i0Pmxuwx-InM5PyY4T26MU5ZF4iygNYZ7ONZtt6-cYsM3lL_jr3uUoUOd_cBu7349uv8Mr-6_v7jfH2VmwrElGMlUXGwKbUoGktCNVbWFoTpRNekxDYdTdsK0XaQSFTSUkMgBVSGSiwP2Mky18fJ6WhcCvpg_DimvLoGWZSVStDHBUqLPG0oTnpw0VDf40h-E3WtGlErNYNiAU3wMQbq9GNwA4Y_moOeZevZpJ5NagV6K1vfpLb3u_mbdiD70rSzm-ofdnWMyVcXcDQu_putRANVM3PHC9eh13gfEnP7swBeQlGnj1WZiK8LQcnps6Mwr0yjIevCvLH17v9R_wKkXqOL</recordid><startdate>19900615</startdate><enddate>19900615</enddate><creator>Rose, Richard C.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19900615</creationdate><title>Ascorbic acid metabolism in protection against free radicals: A radiation model</title><author>Rose, Richard C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-a56a910d034428de498d67d04cf4f8291d8298bb44bf0a91a96de8e06405ce3a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</topic><topic>AMINO ACIDS</topic><topic>ANIMAL TISSUES</topic><topic>ANIMALS</topic><topic>ASCORBIC ACID</topic><topic>Ascorbic Acid - metabolism</topic><topic>Ascorbic Acid - radiation effects</topic><topic>Biological and medical sciences</topic><topic>BIOSYNTHESIS</topic><topic>BODY</topic><topic>CARBOXYLIC ACIDS</topic><topic>Cell physiology</topic><topic>CHEMICAL REACTIONS</topic><topic>COENZYMES</topic><topic>colon</topic><topic>Colon - metabolism</topic><topic>CYSTEINE</topic><topic>Cytosol - enzymology</topic><topic>Dehydroascorbic Acid - metabolism</topic><topic>DIGESTIVE SYSTEM</topic><topic>Effects of physical and chemical agents</topic><topic>electromagnetic radiation</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>EPITHELIUM</topic><topic>Free Radicals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GASTROINTESTINAL TRACT</topic><topic>Humans</topic><topic>Hydroxides</topic><topic>Hydroxyl Radical</topic><topic>Intestinal Mucosa - metabolism</topic><topic>INTESTINES</topic><topic>IONIZING RADIATIONS</topic><topic>Kinetics</topic><topic>LARGE INTESTINE</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>MEMBRANES</topic><topic>METABOLISM</topic><topic>Molecular and cellular biology</topic><topic>MUCOUS MEMBRANES</topic><topic>NADH, NADPH Oxidoreductases - metabolism</topic><topic>NADH2</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC SULFUR COMPOUNDS</topic><topic>ORGANS</topic><topic>Oxidation-Reduction</topic><topic>OXIDOREDUCTASES</topic><topic>PRIMATES</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>RADICALS</topic><topic>RADIOSENSITIVITY EFFECTS</topic><topic>RATS</topic><topic>Rats, Inbred Strains</topic><topic>REDOX REACTIONS</topic><topic>RODENTS</topic><topic>SYNTHESIS</topic><topic>THIOLS</topic><topic>thrombin</topic><topic>TISSUES</topic><topic>VERTEBRATES</topic><topic>VITAMINS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rose, Richard C.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rose, Richard C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ascorbic acid metabolism in protection against free radicals: A radiation model</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1990-06-15</date><risdate>1990</risdate><volume>169</volume><issue>2</issue><spage>430</spage><epage>436</epage><pages>430-436</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>The role of ascorbic acid in scavenging free radicals was evaluated in a model of mammalian colonic epithelium homogenized in physiologic buffer and exposed to ionizing radiation. Ascorbic acid interacts with hydroxyl free radicals, resulting in production of the ascorbate free radical (AḞR). Colonic mucosa contains a soluble factor that is heat sensitive, PCA precipitable and is contained within 1,000 MW dialysis tubing; it uses GSH and cysteine to reduce AḞR. The factor from rat colon is fractionated between 55 and 70% saturation with solid (NH
4)
2SO
4; a 3–4 fold increase in enzyme activity was achieved. We suggest that the factor is a cytosolic enzyme appropriately referred to as soluble AḞR-reductase. This information provides insight into the mechanism by which ascorbic acid protects against damage by hydroxyl free radicals.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2162665</pmid><doi>10.1016/0006-291X(90)90349-R</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1990-06, Vol.169 (2), p.430-436 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_osti_scitechconnect_7062359 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture AMINO ACIDS ANIMAL TISSUES ANIMALS ASCORBIC ACID Ascorbic Acid - metabolism Ascorbic Acid - radiation effects Biological and medical sciences BIOSYNTHESIS BODY CARBOXYLIC ACIDS Cell physiology CHEMICAL REACTIONS COENZYMES colon Colon - metabolism CYSTEINE Cytosol - enzymology Dehydroascorbic Acid - metabolism DIGESTIVE SYSTEM Effects of physical and chemical agents electromagnetic radiation ENZYME ACTIVITY ENZYMES EPITHELIUM Free Radicals Fundamental and applied biological sciences. Psychology GASTROINTESTINAL TRACT Humans Hydroxides Hydroxyl Radical Intestinal Mucosa - metabolism INTESTINES IONIZING RADIATIONS Kinetics LARGE INTESTINE MAMMALS MAN MEMBRANES METABOLISM Molecular and cellular biology MUCOUS MEMBRANES NADH, NADPH Oxidoreductases - metabolism NADH2 NUCLEOTIDES ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS ORGANS Oxidation-Reduction OXIDOREDUCTASES PRIMATES RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RADICALS RADIOSENSITIVITY EFFECTS RATS Rats, Inbred Strains REDOX REACTIONS RODENTS SYNTHESIS THIOLS thrombin TISSUES VERTEBRATES VITAMINS |
| title | Ascorbic acid metabolism in protection against free radicals: A radiation model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T19%3A42%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ascorbic%20acid%20metabolism%20in%20protection%20against%20free%20radicals:%20A%20radiation%20model&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Rose,%20Richard%20C.&rft.date=1990-06-15&rft.volume=169&rft.issue=2&rft.spage=430&rft.epage=436&rft.pages=430-436&rft.issn=0006-291X&rft.eissn=1090-2104&rft.coden=BBRCA9&rft_id=info:doi/10.1016/0006-291X(90)90349-R&rft_dat=%3Cproquest_osti_%3E79847999%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79847999&rft_id=info:pmid/2162665&rft_els_id=0006291X9090349R&rfr_iscdi=true |