The Genes for Oncostatin M (OSM) and Leukemia Inhibitory Factor (LIF) Are Tightly Linked on Human Chromosome 22
Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are members of a family of cytokines that regulate the proliferation and differentiation of a variety of cell types. In this report, cDNA probes specific for OSM and LIF were hybridized to DNA from somatic cell hybrids containing defined region...
Gespeichert in:
| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1993-07, Vol.17 (1), p.136-140 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 140 |
|---|---|
| container_issue | 1 |
| container_start_page | 136 |
| container_title | Genomics (San Diego, Calif.) |
| container_volume | 17 |
| creator | Rose, Timothy M. Lagrou, Michelle J. Fransson, Ingegerd Werelius, Barbro Delattre, Olivier Thomas, Gilles de Jong, Pieter J. Todaro, George J. Dumanski, Jan P. |
| description | Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are members of a family of cytokines that regulate the proliferation and differentiation of a variety of cell types. In this report, cDNA probes specific for OSM and LIF were hybridized to DNA from somatic cell hybrids containing defined regions of human chromosome 22, and the gene for human OSM was found to segregate with that of LIF. Southern analysis of high-molecular-weight DNA that had been digested with rare-cutting restriction enzymes and analyzed by pulsed-field gel electrophoresis showed identical hybridization patterns with both probes. The probes also identified common cosmid clones on high-density cosmid filters prepared from chromosome 22-specific flow-sorted cosmid libraries. Restriction and Southern analyses of six cosmid clones established a contig of approximately 100 kb surrounding the genes for OSM and LIF. The OSM and LIF genes are tandemly arranged in the same transcriptional orientation separated by approximately 10 kb. The direction of gene transcription is telomeric to centromeric, with the OSM gene located upstream of the LIF gene. Our studies define a new gene cluster on chromosome 22 and provide strong evidence that OSM and LIF have resulted from duplication of a common ancestral gene. |
| doi_str_mv | 10.1006/geno.1993.1294 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_7035508</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0888754383712942</els_id><sourcerecordid>75988794</sourcerecordid><originalsourceid>FETCH-LOGICAL-c395t-17b853bac573c51c22e276d78d5e314c5198b8050108bc9f01b97b3fdd422c803</originalsourceid><addsrcrecordid>eNp1kc1r3DAQxU1pSTdpr70VRAkhOXirL9vSMSzdZMFhD92ehSyPYzW2lEp2Yf_7yuySW08DM78Z5r2XZV8IXhOMy-_P4PyaSMnWhEr-LlsRLGQuSl6-z1ZYCJFXBWcfs8sYf2OMJRP0IrsQHJec81XmDz2gB3AQUecD2jvj46Qn69ATut3_fLpD2rWohvkFRqvRzvW2sZMPR7TVJlV0W--2d-g-ADrY534ajqi27gVa5B16nEft0KYPfvTRj4Ao_ZR96PQQ4fO5XmW_tj8Om8e83j_sNvd1bpgsppxUjShYo01RMVMQQynQqmwr0RbACE8tKRqBC5zUNkZ2mDSyaljXtpxSIzC7yr6d7iY5VkVjJzC98c6BmVSFWVFgkaCbE_Qa_J8Z4qRGGw0Mg3bg56iqQgpRSZ7A9Qk0wccYoFOvwY46HBXBaolBLTGoJQa1xJAWvp4vz80I7Rt-9j3Nr89zHY0euqCdsfEN44JyiknCxAmDZNVfC2FRAs5Aa8MipPX2fx_8A9nwoB8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75988794</pqid></control><display><type>article</type><title>The Genes for Oncostatin M (OSM) and Leukemia Inhibitory Factor (LIF) Are Tightly Linked on Human Chromosome 22</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Rose, Timothy M. ; Lagrou, Michelle J. ; Fransson, Ingegerd ; Werelius, Barbro ; Delattre, Olivier ; Thomas, Gilles ; de Jong, Pieter J. ; Todaro, George J. ; Dumanski, Jan P.</creator><creatorcontrib>Rose, Timothy M. ; Lagrou, Michelle J. ; Fransson, Ingegerd ; Werelius, Barbro ; Delattre, Olivier ; Thomas, Gilles ; de Jong, Pieter J. ; Todaro, George J. ; Dumanski, Jan P.</creatorcontrib><description>Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are members of a family of cytokines that regulate the proliferation and differentiation of a variety of cell types. In this report, cDNA probes specific for OSM and LIF were hybridized to DNA from somatic cell hybrids containing defined regions of human chromosome 22, and the gene for human OSM was found to segregate with that of LIF. Southern analysis of high-molecular-weight DNA that had been digested with rare-cutting restriction enzymes and analyzed by pulsed-field gel electrophoresis showed identical hybridization patterns with both probes. The probes also identified common cosmid clones on high-density cosmid filters prepared from chromosome 22-specific flow-sorted cosmid libraries. Restriction and Southern analyses of six cosmid clones established a contig of approximately 100 kb surrounding the genes for OSM and LIF. The OSM and LIF genes are tandemly arranged in the same transcriptional orientation separated by approximately 10 kb. The direction of gene transcription is telomeric to centromeric, with the OSM gene located upstream of the LIF gene. Our studies define a new gene cluster on chromosome 22 and provide strong evidence that OSM and LIF have resulted from duplication of a common ancestral gene.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1993.1294</identifier><identifier>PMID: 8406444</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>550400 - Genetics ; Animals ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; Blotting, Southern ; CHROMOSOMES ; Chromosomes, Human, Pair 22 ; Classical genetics, quantitative genetics, hybrids ; Cosmids ; Cricetinae ; DNA HYBRIDIZATION ; DNA Probes ; DNA, Complementary - genetics ; Fundamental and applied biological sciences. Psychology ; GENES ; GENETIC MAPPING ; Genetics of eukaryotes. Biological and molecular evolution ; GROWTH FACTORS ; Growth Inhibitors - genetics ; Human ; HUMAN CHROMOSOME 22 ; HUMAN CHROMOSOMES ; Humans ; Hybrid Cells ; HYBRIDIZATION ; Interleukin-6 ; Leukemia Inhibitory Factor ; LYMPHOKINES ; Lymphokines - genetics ; MAPPING ; MITOGENS ; Multigene Family ; Oncostatin M ; ORGANIC COMPOUNDS ; Peptides - genetics ; PROTEINS ; Restriction Mapping</subject><ispartof>Genomics (San Diego, Calif.), 1993-07, Vol.17 (1), p.136-140</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-17b853bac573c51c22e276d78d5e314c5198b8050108bc9f01b97b3fdd422c803</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754383712942$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4824201$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8406444$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7035508$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Rose, Timothy M.</creatorcontrib><creatorcontrib>Lagrou, Michelle J.</creatorcontrib><creatorcontrib>Fransson, Ingegerd</creatorcontrib><creatorcontrib>Werelius, Barbro</creatorcontrib><creatorcontrib>Delattre, Olivier</creatorcontrib><creatorcontrib>Thomas, Gilles</creatorcontrib><creatorcontrib>de Jong, Pieter J.</creatorcontrib><creatorcontrib>Todaro, George J.</creatorcontrib><creatorcontrib>Dumanski, Jan P.</creatorcontrib><title>The Genes for Oncostatin M (OSM) and Leukemia Inhibitory Factor (LIF) Are Tightly Linked on Human Chromosome 22</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are members of a family of cytokines that regulate the proliferation and differentiation of a variety of cell types. In this report, cDNA probes specific for OSM and LIF were hybridized to DNA from somatic cell hybrids containing defined regions of human chromosome 22, and the gene for human OSM was found to segregate with that of LIF. Southern analysis of high-molecular-weight DNA that had been digested with rare-cutting restriction enzymes and analyzed by pulsed-field gel electrophoresis showed identical hybridization patterns with both probes. The probes also identified common cosmid clones on high-density cosmid filters prepared from chromosome 22-specific flow-sorted cosmid libraries. Restriction and Southern analyses of six cosmid clones established a contig of approximately 100 kb surrounding the genes for OSM and LIF. The OSM and LIF genes are tandemly arranged in the same transcriptional orientation separated by approximately 10 kb. The direction of gene transcription is telomeric to centromeric, with the OSM gene located upstream of the LIF gene. Our studies define a new gene cluster on chromosome 22 and provide strong evidence that OSM and LIF have resulted from duplication of a common ancestral gene.</description><subject>550400 - Genetics</subject><subject>Animals</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>CHROMOSOMES</subject><subject>Chromosomes, Human, Pair 22</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>Cosmids</subject><subject>Cricetinae</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA Probes</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENES</subject><subject>GENETIC MAPPING</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>GROWTH FACTORS</subject><subject>Growth Inhibitors - genetics</subject><subject>Human</subject><subject>HUMAN CHROMOSOME 22</subject><subject>HUMAN CHROMOSOMES</subject><subject>Humans</subject><subject>Hybrid Cells</subject><subject>HYBRIDIZATION</subject><subject>Interleukin-6</subject><subject>Leukemia Inhibitory Factor</subject><subject>LYMPHOKINES</subject><subject>Lymphokines - genetics</subject><subject>MAPPING</subject><subject>MITOGENS</subject><subject>Multigene Family</subject><subject>Oncostatin M</subject><subject>ORGANIC COMPOUNDS</subject><subject>Peptides - genetics</subject><subject>PROTEINS</subject><subject>Restriction Mapping</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1r3DAQxU1pSTdpr70VRAkhOXirL9vSMSzdZMFhD92ehSyPYzW2lEp2Yf_7yuySW08DM78Z5r2XZV8IXhOMy-_P4PyaSMnWhEr-LlsRLGQuSl6-z1ZYCJFXBWcfs8sYf2OMJRP0IrsQHJec81XmDz2gB3AQUecD2jvj46Qn69ATut3_fLpD2rWohvkFRqvRzvW2sZMPR7TVJlV0W--2d-g-ADrY534ajqi27gVa5B16nEft0KYPfvTRj4Ao_ZR96PQQ4fO5XmW_tj8Om8e83j_sNvd1bpgsppxUjShYo01RMVMQQynQqmwr0RbACE8tKRqBC5zUNkZ2mDSyaljXtpxSIzC7yr6d7iY5VkVjJzC98c6BmVSFWVFgkaCbE_Qa_J8Z4qRGGw0Mg3bg56iqQgpRSZ7A9Qk0wccYoFOvwY46HBXBaolBLTGoJQa1xJAWvp4vz80I7Rt-9j3Nr89zHY0euqCdsfEN44JyiknCxAmDZNVfC2FRAs5Aa8MipPX2fx_8A9nwoB8</recordid><startdate>19930701</startdate><enddate>19930701</enddate><creator>Rose, Timothy M.</creator><creator>Lagrou, Michelle J.</creator><creator>Fransson, Ingegerd</creator><creator>Werelius, Barbro</creator><creator>Delattre, Olivier</creator><creator>Thomas, Gilles</creator><creator>de Jong, Pieter J.</creator><creator>Todaro, George J.</creator><creator>Dumanski, Jan P.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19930701</creationdate><title>The Genes for Oncostatin M (OSM) and Leukemia Inhibitory Factor (LIF) Are Tightly Linked on Human Chromosome 22</title><author>Rose, Timothy M. ; Lagrou, Michelle J. ; Fransson, Ingegerd ; Werelius, Barbro ; Delattre, Olivier ; Thomas, Gilles ; de Jong, Pieter J. ; Todaro, George J. ; Dumanski, Jan P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-17b853bac573c51c22e276d78d5e314c5198b8050108bc9f01b97b3fdd422c803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>550400 - Genetics</topic><topic>Animals</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>CHROMOSOMES</topic><topic>Chromosomes, Human, Pair 22</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>Cosmids</topic><topic>Cricetinae</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA Probes</topic><topic>DNA, Complementary - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENES</topic><topic>GENETIC MAPPING</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>GROWTH FACTORS</topic><topic>Growth Inhibitors - genetics</topic><topic>Human</topic><topic>HUMAN CHROMOSOME 22</topic><topic>HUMAN CHROMOSOMES</topic><topic>Humans</topic><topic>Hybrid Cells</topic><topic>HYBRIDIZATION</topic><topic>Interleukin-6</topic><topic>Leukemia Inhibitory Factor</topic><topic>LYMPHOKINES</topic><topic>Lymphokines - genetics</topic><topic>MAPPING</topic><topic>MITOGENS</topic><topic>Multigene Family</topic><topic>Oncostatin M</topic><topic>ORGANIC COMPOUNDS</topic><topic>Peptides - genetics</topic><topic>PROTEINS</topic><topic>Restriction Mapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rose, Timothy M.</creatorcontrib><creatorcontrib>Lagrou, Michelle J.</creatorcontrib><creatorcontrib>Fransson, Ingegerd</creatorcontrib><creatorcontrib>Werelius, Barbro</creatorcontrib><creatorcontrib>Delattre, Olivier</creatorcontrib><creatorcontrib>Thomas, Gilles</creatorcontrib><creatorcontrib>de Jong, Pieter J.</creatorcontrib><creatorcontrib>Todaro, George J.</creatorcontrib><creatorcontrib>Dumanski, Jan P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rose, Timothy M.</au><au>Lagrou, Michelle J.</au><au>Fransson, Ingegerd</au><au>Werelius, Barbro</au><au>Delattre, Olivier</au><au>Thomas, Gilles</au><au>de Jong, Pieter J.</au><au>Todaro, George J.</au><au>Dumanski, Jan P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Genes for Oncostatin M (OSM) and Leukemia Inhibitory Factor (LIF) Are Tightly Linked on Human Chromosome 22</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1993-07-01</date><risdate>1993</risdate><volume>17</volume><issue>1</issue><spage>136</spage><epage>140</epage><pages>136-140</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>Oncostatin M (OSM) and leukemia inhibitory factor (LIF) are members of a family of cytokines that regulate the proliferation and differentiation of a variety of cell types. In this report, cDNA probes specific for OSM and LIF were hybridized to DNA from somatic cell hybrids containing defined regions of human chromosome 22, and the gene for human OSM was found to segregate with that of LIF. Southern analysis of high-molecular-weight DNA that had been digested with rare-cutting restriction enzymes and analyzed by pulsed-field gel electrophoresis showed identical hybridization patterns with both probes. The probes also identified common cosmid clones on high-density cosmid filters prepared from chromosome 22-specific flow-sorted cosmid libraries. Restriction and Southern analyses of six cosmid clones established a contig of approximately 100 kb surrounding the genes for OSM and LIF. The OSM and LIF genes are tandemly arranged in the same transcriptional orientation separated by approximately 10 kb. The direction of gene transcription is telomeric to centromeric, with the OSM gene located upstream of the LIF gene. Our studies define a new gene cluster on chromosome 22 and provide strong evidence that OSM and LIF have resulted from duplication of a common ancestral gene.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8406444</pmid><doi>10.1006/geno.1993.1294</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0888-7543 |
| ispartof | Genomics (San Diego, Calif.), 1993-07, Vol.17 (1), p.136-140 |
| issn | 0888-7543 1089-8646 |
| language | eng |
| recordid | cdi_osti_scitechconnect_7035508 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 550400 - Genetics Animals BASIC BIOLOGICAL SCIENCES Biological and medical sciences Blotting, Southern CHROMOSOMES Chromosomes, Human, Pair 22 Classical genetics, quantitative genetics, hybrids Cosmids Cricetinae DNA HYBRIDIZATION DNA Probes DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology GENES GENETIC MAPPING Genetics of eukaryotes. Biological and molecular evolution GROWTH FACTORS Growth Inhibitors - genetics Human HUMAN CHROMOSOME 22 HUMAN CHROMOSOMES Humans Hybrid Cells HYBRIDIZATION Interleukin-6 Leukemia Inhibitory Factor LYMPHOKINES Lymphokines - genetics MAPPING MITOGENS Multigene Family Oncostatin M ORGANIC COMPOUNDS Peptides - genetics PROTEINS Restriction Mapping |
| title | The Genes for Oncostatin M (OSM) and Leukemia Inhibitory Factor (LIF) Are Tightly Linked on Human Chromosome 22 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-15T00%3A26%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Genes%20for%20Oncostatin%20M%20(OSM)%20and%20Leukemia%20Inhibitory%20Factor%20(LIF)%20Are%20Tightly%20Linked%20on%20Human%20Chromosome%2022&rft.jtitle=Genomics%20(San%20Diego,%20Calif.)&rft.au=Rose,%20Timothy%20M.&rft.date=1993-07-01&rft.volume=17&rft.issue=1&rft.spage=136&rft.epage=140&rft.pages=136-140&rft.issn=0888-7543&rft.eissn=1089-8646&rft_id=info:doi/10.1006/geno.1993.1294&rft_dat=%3Cproquest_osti_%3E75988794%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75988794&rft_id=info:pmid/8406444&rft_els_id=S0888754383712942&rfr_iscdi=true |