Simultaneous extraction of β-endorphin and Leu- and met-enkephalins from human and rat plasma

A simple, rapid and reliable procedure is described to simultaneously concentrate and purify β-endorphin, leu- and met-enkephalins from small volumes of human and rat plasma before radioimmunoassay is performed. It uses C 18 Sep-Pak reverse phase cartridges. The effectiveness of different protease i...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Life sciences (1973) 1989, Vol.45 (10), p.901-906
Hauptverfasser:	Bhathena, Sam J., Smith, Patricia M., Kennedy, Bruce W., Voyles, Nancy R., Recant, Lillian
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Animals AUTONOMIC NERVOUS SYSTEM AGENTS BASIC BIOLOGICAL SCIENCES BIOASSAY Biological and medical sciences BIOLOGICAL FUNCTIONS Chromatography, Ion Exchange DIAGNOSTIC TECHNIQUES DRUGS ENDORPHINS Endorphins - blood Endorphins - isolation & purification Enkephalin, Leucine - blood Enkephalin, Leucine - isolation & purification Enkephalin, Methionine - blood Enkephalin, Methionine - isolation & purification ENKEPHALINS ENZYME INHIBITORS FRACTIONATION FUNCTIONS Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration IMMUNOASSAY IMMUNOLOGY ISOTOPE APPLICATIONS NEUROREGULATORS ORGANIC COMPOUNDS PEPTIDES POLYPEPTIDES PROTEINS RADIOASSAY RADIOIMMUNOASSAY Radioimmunoassay - methods RADIOIMMUNODETECTION RADIOIMMUNOLOGY Rats SEPARATION PROCESSES Solvents TRACER TECHNIQUES
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A simple, rapid and reliable procedure is described to simultaneously concentrate and purify β-endorphin, leu- and met-enkephalins from small volumes of human and rat plasma before radioimmunoassay is performed. It uses C 18 Sep-Pak reverse phase cartridges. The effectiveness of different protease inhibitors in preventing degradation of opiates by plasma and different solvent systems for eluting opiates is also evaluated.
ISSN:	0024-3205 1879-0631
DOI:	10.1016/0024-3205(89)90204-X