Human DNA topoisomerase II: evaluation of enzyme activity in normal and neoplastic tissues

We have used both a quantitative filter binding assay and a decatenation assay to measure DNA topoisomerase II activity. The filter binding assay, which measures catenating activity, is able to detect topoisomerase II activity at 50-100-fold lower protein concentrations than the decatenation assay....

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Veröffentlicht in:	Biochemistry (Easton) 1990-02, Vol.29 (8), p.2127-2134
Hauptverfasser:	Holden, Joseph A, Rolfson, Debra H, Wittwer, Carl T
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques Adolescent Adult Aged Analytical, structural and metabolic biochemistry ANIMAL CELLS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOCHEMISTRY Biological and medical sciences CARCINOMAS CELL CYCLE CELL FLOW SYSTEMS CHEMISTRY DAYS LIVING RADIOISOTOPES DISEASES DNA Topoisomerases, Type II - metabolism DNA, Bacterial - isolation & purification ENZYME ACTIVITY ENZYMES Enzymes and enzyme inhibitors Escherichia coli - genetics Female Flow Cytometry Fundamental and applied biological sciences. Psychology Genes, Immunoglobulin HELA CELLS Hot Temperature Humans IMMUNE SYSTEM DISEASES ISOMERASES ISOTOPES LIGHT NUCLEI LYMPHOMAS Male Middle Aged NEOPLASMS Neoplasms - enzymology Neoplasms - pathology NUCLEI ODD-ODD NUCLEI Organ Specificity PHOSPHORUS 32 PHOSPHORUS ISOTOPES Placenta - drug effects Placenta - enzymology RADIOISOTOPES Spleen - drug effects Spleen - enzymology Teniposide - pharmacology TUMOR CELLS
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