Genetic Mapping of the Human Growth Hormone-Releasing Factor Gene (GHRF) Using Two Intragenic Polymorphisms Detected by PCR Amplification
We have analyzed the human growth hormone-releasing factor (GHRF) gene by high-resolution restriction mapping of its PCR amplification products. Two intragenic PCR fragment length polymorphisms (PCRFLPs) were detected in introns A and C of the GHRF gene, whose heterozygosities are 40 and 7%, respect...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1994-03, Vol.20 (1), p.132-134 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Jurado, L.A.Pérez Phillips, J.A. Summar, M.L. Mao, J. Weber, J.L. Schaefer, F.V. Hazan, J. Argente, J. |
| description | We have analyzed the human growth hormone-releasing factor (GHRF) gene by high-resolution restriction mapping of its PCR amplification products. Two intragenic PCR fragment length polymorphisms (PCRFLPs) were detected in introns A and C of the GHRF gene, whose heterozygosities are 40 and 7%, respectively. Linkage analysis using the CEPH panel showed that GHRF is linked to several markers on chromosome 20 and assigned the GHRF locus to a region near the centromere between D20S27 (assigned to 20p12.1-p11.23) and D20S16 (assigned to 20q12). These intragenic PCRFLPs and the tightly linked polymorphisms should provide useful markers for linkage studies of (GHRF alleles in familial disorders of growth such as isolated growth hormone deficiency. |
| doi_str_mv | 10.1006/geno.1994.1140 |
| format | Article |
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Two intragenic PCR fragment length polymorphisms (PCRFLPs) were detected in introns A and C of the GHRF gene, whose heterozygosities are 40 and 7%, respectively. Linkage analysis using the CEPH panel showed that GHRF is linked to several markers on chromosome 20 and assigned the GHRF locus to a region near the centromere between D20S27 (assigned to 20p12.1-p11.23) and D20S16 (assigned to 20q12). These intragenic PCRFLPs and the tightly linked polymorphisms should provide useful markers for linkage studies of (GHRF alleles in familial disorders of growth such as isolated growth hormone deficiency.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1994.1140</identifier><identifier>PMID: 8020943</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Base Sequence ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; Chromosome Mapping ; CHROMOSOMES ; Chromosomes, Human, Pair 20 ; Classical genetics, quantitative genetics, hybrids ; DNA, Complementary - genetics ; Fundamental and applied biological sciences. Psychology ; GENE AMPLIFICATION ; GENES ; Genetic Linkage ; GENETIC MAPPING ; Genetic Markers ; Genetics of eukaryotes. Biological and molecular evolution ; Growth Disorders - genetics ; Growth Hormone - deficiency ; Growth Hormone-Releasing Hormone - genetics ; HORMONES ; Human ; HUMAN CHROMOSOMES ; Humans ; Introns ; LIBERINS ; MAPPING ; Molecular Sequence Data ; ORGANIC COMPOUNDS ; PEPTIDE HORMONES ; PITUITARY HORMONES ; POLYMERASE CHAIN REACTION ; Polymorphism, Genetic ; PROTEINS 550400 -- Genetics</subject><ispartof>Genomics (San Diego, Calif.), 1994-03, Vol.20 (1), p.132-134</ispartof><rights>1994 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c310t-cab27c8774f7fefe687d6c4ba9caba7ba522d937f7bc948793810eb8463bc8423</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754384711402$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3994270$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8020943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6821800$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Jurado, L.A.Pérez</creatorcontrib><creatorcontrib>Phillips, J.A.</creatorcontrib><creatorcontrib>Summar, M.L.</creatorcontrib><creatorcontrib>Mao, J.</creatorcontrib><creatorcontrib>Weber, J.L.</creatorcontrib><creatorcontrib>Schaefer, F.V.</creatorcontrib><creatorcontrib>Hazan, J.</creatorcontrib><creatorcontrib>Argente, J.</creatorcontrib><title>Genetic Mapping of the Human Growth Hormone-Releasing Factor Gene (GHRF) Using Two Intragenic Polymorphisms Detected by PCR Amplification</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>We have analyzed the human growth hormone-releasing factor (GHRF) gene by high-resolution restriction mapping of its PCR amplification products. Two intragenic PCR fragment length polymorphisms (PCRFLPs) were detected in introns A and C of the GHRF gene, whose heterozygosities are 40 and 7%, respectively. Linkage analysis using the CEPH panel showed that GHRF is linked to several markers on chromosome 20 and assigned the GHRF locus to a region near the centromere between D20S27 (assigned to 20p12.1-p11.23) and D20S16 (assigned to 20q12). These intragenic PCRFLPs and the tightly linked polymorphisms should provide useful markers for linkage studies of (GHRF alleles in familial disorders of growth such as isolated growth hormone deficiency.</description><subject>Base Sequence</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>CHROMOSOMES</subject><subject>Chromosomes, Human, Pair 20</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>DNA, Complementary - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE AMPLIFICATION</subject><subject>GENES</subject><subject>Genetic Linkage</subject><subject>GENETIC MAPPING</subject><subject>Genetic Markers</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Growth Disorders - genetics</subject><subject>Growth Hormone - deficiency</subject><subject>Growth Hormone-Releasing Hormone - genetics</subject><subject>HORMONES</subject><subject>Human</subject><subject>HUMAN CHROMOSOMES</subject><subject>Humans</subject><subject>Introns</subject><subject>LIBERINS</subject><subject>MAPPING</subject><subject>Molecular Sequence Data</subject><subject>ORGANIC COMPOUNDS</subject><subject>PEPTIDE HORMONES</subject><subject>PITUITARY HORMONES</subject><subject>POLYMERASE CHAIN REACTION</subject><subject>Polymorphism, Genetic</subject><subject>PROTEINS 550400 -- Genetics</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFv0zAYxS0EGqVw5YZkIYTGIcVO3Ng5TmVtJw0xVdvZcpwvq1FiB9tl6p_Af41Nq904-fB-39N7fgi9p2RBCam_PoJ1C9o0bEEpIy_QjBLRFKJm9Us0I0KIgi9Z9Rq9CeEnIaSpRHmBLgQpScOqGfqzAQvRaPxdTZOxj9j1OO4Bbw-jsnjj3VPc463zo7NQ7GAAFTK1Vjo6j_Mxvtxsd-sv-OGfcP_k8I2NXqVcyfXODcfR-WlvwhjwN4igI3S4PeK71Q5fjdNgeqNVNM6-Ra96NQR4d37n6GF9fb_aFrc_Njerq9tCV5TEQqu25FpwznreQw-14F2tWauapCjeqmVZdk3Fe97qhgmeClMCrWB11WrBymqOPp58XYhGBm1Spr121qZoshYlFYQk6PMJmrz7dYAQ5WiChmFQFtwhSF4vU4RkPkeLE6i9C8FDLydvRuWPkhKZB5J5IJkHknmgdPDh7HxoR-ie8fMiSf901lXQaui9stqEZ6xKRiXPNuKEQfqq3wZ8bgJWQ2d8LtI5878EfwGDRqyS</recordid><startdate>19940301</startdate><enddate>19940301</enddate><creator>Jurado, L.A.Pérez</creator><creator>Phillips, J.A.</creator><creator>Summar, M.L.</creator><creator>Mao, J.</creator><creator>Weber, J.L.</creator><creator>Schaefer, F.V.</creator><creator>Hazan, J.</creator><creator>Argente, J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19940301</creationdate><title>Genetic Mapping of the Human Growth Hormone-Releasing Factor Gene (GHRF) Using Two Intragenic Polymorphisms Detected by PCR Amplification</title><author>Jurado, L.A.Pérez ; Phillips, J.A. ; Summar, M.L. ; Mao, J. ; Weber, J.L. ; Schaefer, F.V. ; Hazan, J. ; Argente, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-cab27c8774f7fefe687d6c4ba9caba7ba522d937f7bc948793810eb8463bc8423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Base Sequence</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>CHROMOSOMES</topic><topic>Chromosomes, Human, Pair 20</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>DNA, Complementary - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE AMPLIFICATION</topic><topic>GENES</topic><topic>Genetic Linkage</topic><topic>GENETIC MAPPING</topic><topic>Genetic Markers</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Growth Disorders - genetics</topic><topic>Growth Hormone - deficiency</topic><topic>Growth Hormone-Releasing Hormone - genetics</topic><topic>HORMONES</topic><topic>Human</topic><topic>HUMAN CHROMOSOMES</topic><topic>Humans</topic><topic>Introns</topic><topic>LIBERINS</topic><topic>MAPPING</topic><topic>Molecular Sequence Data</topic><topic>ORGANIC COMPOUNDS</topic><topic>PEPTIDE HORMONES</topic><topic>PITUITARY HORMONES</topic><topic>POLYMERASE CHAIN REACTION</topic><topic>Polymorphism, Genetic</topic><topic>PROTEINS 550400 -- Genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jurado, L.A.Pérez</creatorcontrib><creatorcontrib>Phillips, J.A.</creatorcontrib><creatorcontrib>Summar, M.L.</creatorcontrib><creatorcontrib>Mao, J.</creatorcontrib><creatorcontrib>Weber, J.L.</creatorcontrib><creatorcontrib>Schaefer, F.V.</creatorcontrib><creatorcontrib>Hazan, J.</creatorcontrib><creatorcontrib>Argente, J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jurado, L.A.Pérez</au><au>Phillips, J.A.</au><au>Summar, M.L.</au><au>Mao, J.</au><au>Weber, J.L.</au><au>Schaefer, F.V.</au><au>Hazan, J.</au><au>Argente, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Mapping of the Human Growth Hormone-Releasing Factor Gene (GHRF) Using Two Intragenic Polymorphisms Detected by PCR Amplification</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1994-03-01</date><risdate>1994</risdate><volume>20</volume><issue>1</issue><spage>132</spage><epage>134</epage><pages>132-134</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>We have analyzed the human growth hormone-releasing factor (GHRF) gene by high-resolution restriction mapping of its PCR amplification products. Two intragenic PCR fragment length polymorphisms (PCRFLPs) were detected in introns A and C of the GHRF gene, whose heterozygosities are 40 and 7%, respectively. Linkage analysis using the CEPH panel showed that GHRF is linked to several markers on chromosome 20 and assigned the GHRF locus to a region near the centromere between D20S27 (assigned to 20p12.1-p11.23) and D20S16 (assigned to 20q12). These intragenic PCRFLPs and the tightly linked polymorphisms should provide useful markers for linkage studies of (GHRF alleles in familial disorders of growth such as isolated growth hormone deficiency.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8020943</pmid><doi>10.1006/geno.1994.1140</doi><tpages>3</tpages></addata></record> |
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| ispartof | Genomics (San Diego, Calif.), 1994-03, Vol.20 (1), p.132-134 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Base Sequence BASIC BIOLOGICAL SCIENCES Biological and medical sciences Chromosome Mapping CHROMOSOMES Chromosomes, Human, Pair 20 Classical genetics, quantitative genetics, hybrids DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology GENE AMPLIFICATION GENES Genetic Linkage GENETIC MAPPING Genetic Markers Genetics of eukaryotes. Biological and molecular evolution Growth Disorders - genetics Growth Hormone - deficiency Growth Hormone-Releasing Hormone - genetics HORMONES Human HUMAN CHROMOSOMES Humans Introns LIBERINS MAPPING Molecular Sequence Data ORGANIC COMPOUNDS PEPTIDE HORMONES PITUITARY HORMONES POLYMERASE CHAIN REACTION Polymorphism, Genetic PROTEINS 550400 -- Genetics |
| title | Genetic Mapping of the Human Growth Hormone-Releasing Factor Gene (GHRF) Using Two Intragenic Polymorphisms Detected by PCR Amplification |
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