Sigma and opioid receptors in human brain tumors
Human brain tumors (obtained as surgical specimens) and nude mouse-borne human neuroblastomas and gliomas were analyzed for sigma and opioid receptor content. Sigma binding was assessed using [ 3H] 1, 3-di- o -tolylguanidine (DTG), whereas opioid receptor subtypes were measured with tritiated forms...
Gespeichert in:
| Veröffentlicht in: | Life sciences (1973) 1990, Vol.46 (18), p.1279-1286 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1286 |
|---|---|
| container_issue | 18 |
| container_start_page | 1279 |
| container_title | Life sciences (1973) |
| container_volume | 46 |
| creator | Thomas, Gail E. Szücs, Maria Mamone, James Y. Bem, Wojciech T. Rush, Michael D. Johnson, Frank E. Coscia, Carmine J. |
| description | Human brain tumors (obtained as surgical specimens) and nude mouse-borne human neuroblastomas and gliomas were analyzed for sigma and opioid receptor content. Sigma binding was assessed using [
3H] 1, 3-di-
o
-tolylguanidine (DTG), whereas opioid receptor subtypes were measured with tritiated forms of the following: μ, [D-ala
2, mePhe
4, gly-ol
5] enkephalin (DAMGE); κ, ethylketocyclazocine (EKC) or U69, 593; δ, [D-pen
2, D-pen
5] enkephalin (DPDPE) or [D-ala
2, D-leu
5] enkephalin (DADLE) with μ suppressor present. Binding parameters were estimated by homologous displacement assays followed by analysis using the LIGAND program. Sigma binding was detected in 15 of 16 tumors examined with very high levels (pmol/mg protein) found in a brain metastasis from an adenocarcinoma of lung and a human neuroblastoma (SK-N-MC) passaged in nude mice. κ opioid receptor binding was detected in 4 of 4 glioblastoma multiforme specimens and 2 of 2 human astrocytoma cell lines tested but not in the other brain tumors analyzed. |
| doi_str_mv | 10.1016/0024-3205(90)90360-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_6699245</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0024320590903604</els_id><sourcerecordid>79797728</sourcerecordid><originalsourceid>FETCH-LOGICAL-c328t-fac9f0ff9f57a7107e78457dd7918e293f4c78ba1b00f8a149b87dce68fc1fbf3</originalsourceid><addsrcrecordid>eNp9kMFq3DAQQEVp2Ww3-YMGTAmhPTgZ2bIlXQJhaZPCQg9JzkKWR12FtbWR7ED-PnK9bG9FhxEzb4aZR8gXClcUaH0NULC8LKD6JuG7hLKGnH0gSyq4zKEu6UeyPCIn5HOMzwBQVbxckAWVnHIolgQe3J9OZ7pvM7933rVZQIP7wYeYuT7bjp3usybo9B_GLmVPySerdxHPDnFFnn7-eFzf55vfd7_Wt5vclIUYcquNtGCttBXXnAJHLljF25ZLKrCQpWWGi0bTBsAKTZlsBG8N1sIaahtbrsjXea6Pg1PRuAHN1vi-RzOoupayYFWCLmdoH_zLiHFQnYsGdzvdox-j4jI9XogEshk0wccY0Kp9cJ0Ob4qCmmyqSZWaVCkJ6q9NxVLb-WH-2HTY_mua9aX6xaGuo9E7G3RvXDxirKpZSeuE3cwYJmGvDsN0D_YGWxemc1rv_r_HO1MYjus</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79797728</pqid></control><display><type>article</type><title>Sigma and opioid receptors in human brain tumors</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Thomas, Gail E. ; Szücs, Maria ; Mamone, James Y. ; Bem, Wojciech T. ; Rush, Michael D. ; Johnson, Frank E. ; Coscia, Carmine J.</creator><creatorcontrib>Thomas, Gail E. ; Szücs, Maria ; Mamone, James Y. ; Bem, Wojciech T. ; Rush, Michael D. ; Johnson, Frank E. ; Coscia, Carmine J.</creatorcontrib><description>Human brain tumors (obtained as surgical specimens) and nude mouse-borne human neuroblastomas and gliomas were analyzed for sigma and opioid receptor content. Sigma binding was assessed using [
3H] 1, 3-di-
o
-tolylguanidine (DTG), whereas opioid receptor subtypes were measured with tritiated forms of the following: μ, [D-ala
2, mePhe
4, gly-ol
5] enkephalin (DAMGE); κ, ethylketocyclazocine (EKC) or U69, 593; δ, [D-pen
2, D-pen
5] enkephalin (DPDPE) or [D-ala
2, D-leu
5] enkephalin (DADLE) with μ suppressor present. Binding parameters were estimated by homologous displacement assays followed by analysis using the LIGAND program. Sigma binding was detected in 15 of 16 tumors examined with very high levels (pmol/mg protein) found in a brain metastasis from an adenocarcinoma of lung and a human neuroblastoma (SK-N-MC) passaged in nude mice. κ opioid receptor binding was detected in 4 of 4 glioblastoma multiforme specimens and 2 of 2 human astrocytoma cell lines tested but not in the other brain tumors analyzed.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(90)90360-4</identifier><identifier>PMID: 1971702</identifier><identifier>CODEN: LIFSAK</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>550901 -- Pathology-- Tracer Techniques ; Adenocarcinoma - analysis ; Adenocarcinoma - secondary ; Analgesics, Opioid - metabolism ; ANIMALS ; Astrocytoma - analysis ; AUTONOMIC NERVOUS SYSTEM AGENTS ; BASIC BIOLOGICAL SCIENCES ; BIOCHEMICAL REACTION KINETICS ; Biological and medical sciences ; BODY ; BRAIN ; Brain Neoplasms - analysis ; Brain Neoplasms - secondary ; CENTRAL NERVOUS SYSTEM ; COMPUTER CODES ; Cyclazocine - analogs & derivatives ; Cyclazocine - metabolism ; DISEASES ; DRUGS ; ENDORPHINS ; Ethylketocyclazocine ; Glioblastoma - analysis ; Glioma - analysis ; Humans ; HYDROGEN COMPOUNDS ; ISOTOPE APPLICATIONS ; KINETICS ; L CODES ; Male ; MAMMALS ; MAN ; Medical sciences ; MEMBRANE PROTEINS ; METASTASES ; Mice ; Mice, Nude ; NEOPLASMS ; NERVOUS SYSTEM ; Neuroblastoma - analysis ; NEUROREGULATORS ; ORGANIC COMPOUNDS ; ORGANS ; PRIMATES ; PROTEINS ; Radioligand Assay ; Rats ; REACTION KINETICS ; RECEPTORS ; Receptors, Opioid - analysis ; Receptors, sigma ; TRACER TECHNIQUES ; TRITIUM COMPOUNDS ; Tumors ; VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</subject><ispartof>Life sciences (1973), 1990, Vol.46 (18), p.1279-1286</ispartof><rights>1990</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c328t-fac9f0ff9f57a7107e78457dd7918e293f4c78ba1b00f8a149b87dce68fc1fbf3</citedby><cites>FETCH-LOGICAL-c328t-fac9f0ff9f57a7107e78457dd7918e293f4c78ba1b00f8a149b87dce68fc1fbf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0024-3205(90)90360-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,4022,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4564316$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1971702$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6699245$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomas, Gail E.</creatorcontrib><creatorcontrib>Szücs, Maria</creatorcontrib><creatorcontrib>Mamone, James Y.</creatorcontrib><creatorcontrib>Bem, Wojciech T.</creatorcontrib><creatorcontrib>Rush, Michael D.</creatorcontrib><creatorcontrib>Johnson, Frank E.</creatorcontrib><creatorcontrib>Coscia, Carmine J.</creatorcontrib><title>Sigma and opioid receptors in human brain tumors</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Human brain tumors (obtained as surgical specimens) and nude mouse-borne human neuroblastomas and gliomas were analyzed for sigma and opioid receptor content. Sigma binding was assessed using [
3H] 1, 3-di-
o
-tolylguanidine (DTG), whereas opioid receptor subtypes were measured with tritiated forms of the following: μ, [D-ala
2, mePhe
4, gly-ol
5] enkephalin (DAMGE); κ, ethylketocyclazocine (EKC) or U69, 593; δ, [D-pen
2, D-pen
5] enkephalin (DPDPE) or [D-ala
2, D-leu
5] enkephalin (DADLE) with μ suppressor present. Binding parameters were estimated by homologous displacement assays followed by analysis using the LIGAND program. Sigma binding was detected in 15 of 16 tumors examined with very high levels (pmol/mg protein) found in a brain metastasis from an adenocarcinoma of lung and a human neuroblastoma (SK-N-MC) passaged in nude mice. κ opioid receptor binding was detected in 4 of 4 glioblastoma multiforme specimens and 2 of 2 human astrocytoma cell lines tested but not in the other brain tumors analyzed.</description><subject>550901 -- Pathology-- Tracer Techniques</subject><subject>Adenocarcinoma - analysis</subject><subject>Adenocarcinoma - secondary</subject><subject>Analgesics, Opioid - metabolism</subject><subject>ANIMALS</subject><subject>Astrocytoma - analysis</subject><subject>AUTONOMIC NERVOUS SYSTEM AGENTS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOCHEMICAL REACTION KINETICS</subject><subject>Biological and medical sciences</subject><subject>BODY</subject><subject>BRAIN</subject><subject>Brain Neoplasms - analysis</subject><subject>Brain Neoplasms - secondary</subject><subject>CENTRAL NERVOUS SYSTEM</subject><subject>COMPUTER CODES</subject><subject>Cyclazocine - analogs & derivatives</subject><subject>Cyclazocine - metabolism</subject><subject>DISEASES</subject><subject>DRUGS</subject><subject>ENDORPHINS</subject><subject>Ethylketocyclazocine</subject><subject>Glioblastoma - analysis</subject><subject>Glioma - analysis</subject><subject>Humans</subject><subject>HYDROGEN COMPOUNDS</subject><subject>ISOTOPE APPLICATIONS</subject><subject>KINETICS</subject><subject>L CODES</subject><subject>Male</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>Medical sciences</subject><subject>MEMBRANE PROTEINS</subject><subject>METASTASES</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>NEOPLASMS</subject><subject>NERVOUS SYSTEM</subject><subject>Neuroblastoma - analysis</subject><subject>NEUROREGULATORS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PRIMATES</subject><subject>PROTEINS</subject><subject>Radioligand Assay</subject><subject>Rats</subject><subject>REACTION KINETICS</subject><subject>RECEPTORS</subject><subject>Receptors, Opioid - analysis</subject><subject>Receptors, sigma</subject><subject>TRACER TECHNIQUES</subject><subject>TRITIUM COMPOUNDS</subject><subject>Tumors</subject><subject>VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFq3DAQQEVp2Ww3-YMGTAmhPTgZ2bIlXQJhaZPCQg9JzkKWR12FtbWR7ED-PnK9bG9FhxEzb4aZR8gXClcUaH0NULC8LKD6JuG7hLKGnH0gSyq4zKEu6UeyPCIn5HOMzwBQVbxckAWVnHIolgQe3J9OZ7pvM7933rVZQIP7wYeYuT7bjp3usybo9B_GLmVPySerdxHPDnFFnn7-eFzf55vfd7_Wt5vclIUYcquNtGCttBXXnAJHLljF25ZLKrCQpWWGi0bTBsAKTZlsBG8N1sIaahtbrsjXea6Pg1PRuAHN1vi-RzOoupayYFWCLmdoH_zLiHFQnYsGdzvdox-j4jI9XogEshk0wccY0Kp9cJ0Ob4qCmmyqSZWaVCkJ6q9NxVLb-WH-2HTY_mua9aX6xaGuo9E7G3RvXDxirKpZSeuE3cwYJmGvDsN0D_YGWxemc1rv_r_HO1MYjus</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Thomas, Gail E.</creator><creator>Szücs, Maria</creator><creator>Mamone, James Y.</creator><creator>Bem, Wojciech T.</creator><creator>Rush, Michael D.</creator><creator>Johnson, Frank E.</creator><creator>Coscia, Carmine J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1990</creationdate><title>Sigma and opioid receptors in human brain tumors</title><author>Thomas, Gail E. ; Szücs, Maria ; Mamone, James Y. ; Bem, Wojciech T. ; Rush, Michael D. ; Johnson, Frank E. ; Coscia, Carmine J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-fac9f0ff9f57a7107e78457dd7918e293f4c78ba1b00f8a149b87dce68fc1fbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>550901 -- Pathology-- Tracer Techniques</topic><topic>Adenocarcinoma - analysis</topic><topic>Adenocarcinoma - secondary</topic><topic>Analgesics, Opioid - metabolism</topic><topic>ANIMALS</topic><topic>Astrocytoma - analysis</topic><topic>AUTONOMIC NERVOUS SYSTEM AGENTS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOCHEMICAL REACTION KINETICS</topic><topic>Biological and medical sciences</topic><topic>BODY</topic><topic>BRAIN</topic><topic>Brain Neoplasms - analysis</topic><topic>Brain Neoplasms - secondary</topic><topic>CENTRAL NERVOUS SYSTEM</topic><topic>COMPUTER CODES</topic><topic>Cyclazocine - analogs & derivatives</topic><topic>Cyclazocine - metabolism</topic><topic>DISEASES</topic><topic>DRUGS</topic><topic>ENDORPHINS</topic><topic>Ethylketocyclazocine</topic><topic>Glioblastoma - analysis</topic><topic>Glioma - analysis</topic><topic>Humans</topic><topic>HYDROGEN COMPOUNDS</topic><topic>ISOTOPE APPLICATIONS</topic><topic>KINETICS</topic><topic>L CODES</topic><topic>Male</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>Medical sciences</topic><topic>MEMBRANE PROTEINS</topic><topic>METASTASES</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>NEOPLASMS</topic><topic>NERVOUS SYSTEM</topic><topic>Neuroblastoma - analysis</topic><topic>NEUROREGULATORS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PRIMATES</topic><topic>PROTEINS</topic><topic>Radioligand Assay</topic><topic>Rats</topic><topic>REACTION KINETICS</topic><topic>RECEPTORS</topic><topic>Receptors, Opioid - analysis</topic><topic>Receptors, sigma</topic><topic>TRACER TECHNIQUES</topic><topic>TRITIUM COMPOUNDS</topic><topic>Tumors</topic><topic>VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomas, Gail E.</creatorcontrib><creatorcontrib>Szücs, Maria</creatorcontrib><creatorcontrib>Mamone, James Y.</creatorcontrib><creatorcontrib>Bem, Wojciech T.</creatorcontrib><creatorcontrib>Rush, Michael D.</creatorcontrib><creatorcontrib>Johnson, Frank E.</creatorcontrib><creatorcontrib>Coscia, Carmine J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomas, Gail E.</au><au>Szücs, Maria</au><au>Mamone, James Y.</au><au>Bem, Wojciech T.</au><au>Rush, Michael D.</au><au>Johnson, Frank E.</au><au>Coscia, Carmine J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sigma and opioid receptors in human brain tumors</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1990</date><risdate>1990</risdate><volume>46</volume><issue>18</issue><spage>1279</spage><epage>1286</epage><pages>1279-1286</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><coden>LIFSAK</coden><abstract>Human brain tumors (obtained as surgical specimens) and nude mouse-borne human neuroblastomas and gliomas were analyzed for sigma and opioid receptor content. Sigma binding was assessed using [
3H] 1, 3-di-
o
-tolylguanidine (DTG), whereas opioid receptor subtypes were measured with tritiated forms of the following: μ, [D-ala
2, mePhe
4, gly-ol
5] enkephalin (DAMGE); κ, ethylketocyclazocine (EKC) or U69, 593; δ, [D-pen
2, D-pen
5] enkephalin (DPDPE) or [D-ala
2, D-leu
5] enkephalin (DADLE) with μ suppressor present. Binding parameters were estimated by homologous displacement assays followed by analysis using the LIGAND program. Sigma binding was detected in 15 of 16 tumors examined with very high levels (pmol/mg protein) found in a brain metastasis from an adenocarcinoma of lung and a human neuroblastoma (SK-N-MC) passaged in nude mice. κ opioid receptor binding was detected in 4 of 4 glioblastoma multiforme specimens and 2 of 2 human astrocytoma cell lines tested but not in the other brain tumors analyzed.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>1971702</pmid><doi>10.1016/0024-3205(90)90360-4</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 1990, Vol.46 (18), p.1279-1286 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_osti_scitechconnect_6699245 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 550901 -- Pathology-- Tracer Techniques Adenocarcinoma - analysis Adenocarcinoma - secondary Analgesics, Opioid - metabolism ANIMALS Astrocytoma - analysis AUTONOMIC NERVOUS SYSTEM AGENTS BASIC BIOLOGICAL SCIENCES BIOCHEMICAL REACTION KINETICS Biological and medical sciences BODY BRAIN Brain Neoplasms - analysis Brain Neoplasms - secondary CENTRAL NERVOUS SYSTEM COMPUTER CODES Cyclazocine - analogs & derivatives Cyclazocine - metabolism DISEASES DRUGS ENDORPHINS Ethylketocyclazocine Glioblastoma - analysis Glioma - analysis Humans HYDROGEN COMPOUNDS ISOTOPE APPLICATIONS KINETICS L CODES Male MAMMALS MAN Medical sciences MEMBRANE PROTEINS METASTASES Mice Mice, Nude NEOPLASMS NERVOUS SYSTEM Neuroblastoma - analysis NEUROREGULATORS ORGANIC COMPOUNDS ORGANS PRIMATES PROTEINS Radioligand Assay Rats REACTION KINETICS RECEPTORS Receptors, Opioid - analysis Receptors, sigma TRACER TECHNIQUES TRITIUM COMPOUNDS Tumors VERTEBRATES 550201 -- Biochemistry-- Tracer Techniques |
| title | Sigma and opioid receptors in human brain tumors |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T05%3A29%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sigma%20and%20opioid%20receptors%20in%20human%20brain%20tumors&rft.jtitle=Life%20sciences%20(1973)&rft.au=Thomas,%20Gail%20E.&rft.date=1990&rft.volume=46&rft.issue=18&rft.spage=1279&rft.epage=1286&rft.pages=1279-1286&rft.issn=0024-3205&rft.eissn=1879-0631&rft.coden=LIFSAK&rft_id=info:doi/10.1016/0024-3205(90)90360-4&rft_dat=%3Cproquest_osti_%3E79797728%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=79797728&rft_id=info:pmid/1971702&rft_els_id=0024320590903604&rfr_iscdi=true |