Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas
Fifty-one radiation-induced murine osteosarcomas were investigated for alterations in c-myc gene structure and c-myc expression. Amplification of c-myc was found in 30% of BALB/c tumors and 13% of NMRI tumors. A region of common proviral integration, Mlvi-1, localized on the same region on chromosom...
Gespeichert in:
| Veröffentlicht in: | Cancer research (Chicago, Ill.) Ill.), 1990-07, Vol.50 (13), p.4146-4153 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4153 |
|---|---|
| container_issue | 13 |
| container_start_page | 4146 |
| container_title | Cancer research (Chicago, Ill.) |
| container_volume | 50 |
| creator | STURM, S. A STRAUSS, P. G ADOLPH, S HAMEISTER, H ERFLE, V |
| description | Fifty-one radiation-induced murine osteosarcomas were investigated for alterations in c-myc gene structure and c-myc expression. Amplification of c-myc was found in 30% of BALB/c tumors and 13% of NMRI tumors. A region of common proviral integration, Mlvi-1, localized on the same region on chromosome 15, was amplified concomitantly. Multiple copies of both loci were localized on double minutes. Three of the tumors with c-myc amplification also showed rearrangements of the c-myc gene region. One of these rearrangements included the 5' and 3'-flanking sequences and the noncoding part of the third exon. Repetitive sequences were found in the 5' region of the c-myc gene, and the 3' flanking region was substituted by sequences normally present in a more distant part of chromosome 15. Increased levels of c-myc transcripts of apparently normal size were found in tumors carrying amplified c-myc sequences. Abnormally high expression of c-myc in some tumors was correlated with an early stage of osteogenic differentiation, suggesting the involvement of the c-myc gene in the control of the osteogenic differentiation of transformed cells. |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_6694070</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2141296</sourcerecordid><originalsourceid>FETCH-LOGICAL-o263t-4ec2e21063a9c256dd8057c9103a5f9626a220224423f5e71498d110c5f67ed13</originalsourceid><addsrcrecordid>eNo9kE1LAzEQhoMotVZ_ghAEj4F87-ZYil9QEETPS5wkGmmSkuwe-u9dbPE0DM_DO8x7hpZMiZ50UqpztKSU9kTJjl-iq9Z-5lUxqhZowZlk3Oglelun_S6GCHaMJWObHa7e1mrzl08-j7gEDCQdAMeMq3XxzyMxuwm8w2mqMXtc2uhLsxVKsu0aXQS7a_7mNFfo4_HhffNMtq9PL5v1lhSuxUikB-45o1pYA1xp53qqOjCMCquC0VxbzinnUnIRlO-YNL1jjIIKuvOOiRW6O-bO1-PQII4evqHk7GEctDaSdnSWbo_SfvpM3g37GpOth-FUwMzvT9w2sLswPw6x_WvMCK61NOIXS3lkEA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas</title><source>MEDLINE</source><source>American Association for Cancer Research</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>STURM, S. A ; STRAUSS, P. G ; ADOLPH, S ; HAMEISTER, H ; ERFLE, V</creator><creatorcontrib>STURM, S. A ; STRAUSS, P. G ; ADOLPH, S ; HAMEISTER, H ; ERFLE, V</creatorcontrib><description>Fifty-one radiation-induced murine osteosarcomas were investigated for alterations in c-myc gene structure and c-myc expression. Amplification of c-myc was found in 30% of BALB/c tumors and 13% of NMRI tumors. A region of common proviral integration, Mlvi-1, localized on the same region on chromosome 15, was amplified concomitantly. Multiple copies of both loci were localized on double minutes. Three of the tumors with c-myc amplification also showed rearrangements of the c-myc gene region. One of these rearrangements included the 5' and 3'-flanking sequences and the noncoding part of the third exon. Repetitive sequences were found in the 5' region of the c-myc gene, and the 3' flanking region was substituted by sequences normally present in a more distant part of chromosome 15. Increased levels of c-myc transcripts of apparently normal size were found in tumors carrying amplified c-myc sequences. Abnormally high expression of c-myc in some tumors was correlated with an early stage of osteogenic differentiation, suggesting the involvement of the c-myc gene in the control of the osteogenic differentiation of transformed cells.</description><identifier>ISSN: 0008-5472</identifier><identifier>EISSN: 1538-7445</identifier><identifier>PMID: 2141296</identifier><identifier>CODEN: CNREA8</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Animal tumors. Experimental tumors ; ANIMALS ; Biological and medical sciences ; BIOLOGICAL FUNCTIONS ; Chromosome Mapping ; CHROMOSOMES ; DISEASES ; Experimental bone, joint and muscle tumors ; EXPERIMENTAL NEOPLASMS ; Female ; FUNCTIONS ; GENE AMPLIFICATION ; Gene Amplification - genetics ; Gene Rearrangement - genetics ; GENE RECOMBINATION ; GENE RECOMBINATION PROTEINS ; GENE REGULATION ; GENES ; Genomic Library ; GLYCOPROTEINS ; KARYOTYPE ; Karyotyping ; MAMMALS ; Medical sciences ; MICE ; Mice, Inbred BALB C ; NEOPLASMS ; Neoplasms, Radiation-Induced - etiology ; Neoplasms, Radiation-Induced - genetics ; NUCLEIC ACIDS ; NUCLEOPROTEINS ; ONCOGENES ; ORGANIC COMPOUNDS ; Osteopontin ; Osteosarcoma - etiology ; Osteosarcoma - genetics ; OSTEOSARCOMAS ; PROTEINS ; Proto-Oncogene Proteins - analysis ; Proto-Oncogene Proteins c-fos ; Proto-Oncogene Proteins c-mos ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIOINDUCTION ; RNA ; RNA, Neoplasm - analysis ; RODENTS ; SARCOMAS ; Sialoglycoproteins - analysis ; SKELETAL DISEASES ; TRANSCRIPTION ; Tumors ; VERTEBRATES 560152 -- Radiation Effects on Animals-- Animals</subject><ispartof>Cancer research (Chicago, Ill.), 1990-07, Vol.50 (13), p.4146-4153</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19326649$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2141296$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6694070$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>STURM, S. A</creatorcontrib><creatorcontrib>STRAUSS, P. G</creatorcontrib><creatorcontrib>ADOLPH, S</creatorcontrib><creatorcontrib>HAMEISTER, H</creatorcontrib><creatorcontrib>ERFLE, V</creatorcontrib><title>Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas</title><title>Cancer research (Chicago, Ill.)</title><addtitle>Cancer Res</addtitle><description>Fifty-one radiation-induced murine osteosarcomas were investigated for alterations in c-myc gene structure and c-myc expression. Amplification of c-myc was found in 30% of BALB/c tumors and 13% of NMRI tumors. A region of common proviral integration, Mlvi-1, localized on the same region on chromosome 15, was amplified concomitantly. Multiple copies of both loci were localized on double minutes. Three of the tumors with c-myc amplification also showed rearrangements of the c-myc gene region. One of these rearrangements included the 5' and 3'-flanking sequences and the noncoding part of the third exon. Repetitive sequences were found in the 5' region of the c-myc gene, and the 3' flanking region was substituted by sequences normally present in a more distant part of chromosome 15. Increased levels of c-myc transcripts of apparently normal size were found in tumors carrying amplified c-myc sequences. Abnormally high expression of c-myc in some tumors was correlated with an early stage of osteogenic differentiation, suggesting the involvement of the c-myc gene in the control of the osteogenic differentiation of transformed cells.</description><subject>Animal tumors. Experimental tumors</subject><subject>ANIMALS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL FUNCTIONS</subject><subject>Chromosome Mapping</subject><subject>CHROMOSOMES</subject><subject>DISEASES</subject><subject>Experimental bone, joint and muscle tumors</subject><subject>EXPERIMENTAL NEOPLASMS</subject><subject>Female</subject><subject>FUNCTIONS</subject><subject>GENE AMPLIFICATION</subject><subject>Gene Amplification - genetics</subject><subject>Gene Rearrangement - genetics</subject><subject>GENE RECOMBINATION</subject><subject>GENE RECOMBINATION PROTEINS</subject><subject>GENE REGULATION</subject><subject>GENES</subject><subject>Genomic Library</subject><subject>GLYCOPROTEINS</subject><subject>KARYOTYPE</subject><subject>Karyotyping</subject><subject>MAMMALS</subject><subject>Medical sciences</subject><subject>MICE</subject><subject>Mice, Inbred BALB C</subject><subject>NEOPLASMS</subject><subject>Neoplasms, Radiation-Induced - etiology</subject><subject>Neoplasms, Radiation-Induced - genetics</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOPROTEINS</subject><subject>ONCOGENES</subject><subject>ORGANIC COMPOUNDS</subject><subject>Osteopontin</subject><subject>Osteosarcoma - etiology</subject><subject>Osteosarcoma - genetics</subject><subject>OSTEOSARCOMAS</subject><subject>PROTEINS</subject><subject>Proto-Oncogene Proteins - analysis</subject><subject>Proto-Oncogene Proteins c-fos</subject><subject>Proto-Oncogene Proteins c-mos</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIOINDUCTION</subject><subject>RNA</subject><subject>RNA, Neoplasm - analysis</subject><subject>RODENTS</subject><subject>SARCOMAS</subject><subject>Sialoglycoproteins - analysis</subject><subject>SKELETAL DISEASES</subject><subject>TRANSCRIPTION</subject><subject>Tumors</subject><subject>VERTEBRATES 560152 -- Radiation Effects on Animals-- Animals</subject><issn>0008-5472</issn><issn>1538-7445</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LAzEQhoMotVZ_ghAEj4F87-ZYil9QEETPS5wkGmmSkuwe-u9dbPE0DM_DO8x7hpZMiZ50UqpztKSU9kTJjl-iq9Z-5lUxqhZowZlk3Oglelun_S6GCHaMJWObHa7e1mrzl08-j7gEDCQdAMeMq3XxzyMxuwm8w2mqMXtc2uhLsxVKsu0aXQS7a_7mNFfo4_HhffNMtq9PL5v1lhSuxUikB-45o1pYA1xp53qqOjCMCquC0VxbzinnUnIRlO-YNL1jjIIKuvOOiRW6O-bO1-PQII4evqHk7GEctDaSdnSWbo_SfvpM3g37GpOth-FUwMzvT9w2sLswPw6x_WvMCK61NOIXS3lkEA</recordid><startdate>19900701</startdate><enddate>19900701</enddate><creator>STURM, S. A</creator><creator>STRAUSS, P. G</creator><creator>ADOLPH, S</creator><creator>HAMEISTER, H</creator><creator>ERFLE, V</creator><general>American Association for Cancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>OTOTI</scope></search><sort><creationdate>19900701</creationdate><title>Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas</title><author>STURM, S. A ; STRAUSS, P. G ; ADOLPH, S ; HAMEISTER, H ; ERFLE, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-o263t-4ec2e21063a9c256dd8057c9103a5f9626a220224423f5e71498d110c5f67ed13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animal tumors. Experimental tumors</topic><topic>ANIMALS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL FUNCTIONS</topic><topic>Chromosome Mapping</topic><topic>CHROMOSOMES</topic><topic>DISEASES</topic><topic>Experimental bone, joint and muscle tumors</topic><topic>EXPERIMENTAL NEOPLASMS</topic><topic>Female</topic><topic>FUNCTIONS</topic><topic>GENE AMPLIFICATION</topic><topic>Gene Amplification - genetics</topic><topic>Gene Rearrangement - genetics</topic><topic>GENE RECOMBINATION</topic><topic>GENE RECOMBINATION PROTEINS</topic><topic>GENE REGULATION</topic><topic>GENES</topic><topic>Genomic Library</topic><topic>GLYCOPROTEINS</topic><topic>KARYOTYPE</topic><topic>Karyotyping</topic><topic>MAMMALS</topic><topic>Medical sciences</topic><topic>MICE</topic><topic>Mice, Inbred BALB C</topic><topic>NEOPLASMS</topic><topic>Neoplasms, Radiation-Induced - etiology</topic><topic>Neoplasms, Radiation-Induced - genetics</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOPROTEINS</topic><topic>ONCOGENES</topic><topic>ORGANIC COMPOUNDS</topic><topic>Osteopontin</topic><topic>Osteosarcoma - etiology</topic><topic>Osteosarcoma - genetics</topic><topic>OSTEOSARCOMAS</topic><topic>PROTEINS</topic><topic>Proto-Oncogene Proteins - analysis</topic><topic>Proto-Oncogene Proteins c-fos</topic><topic>Proto-Oncogene Proteins c-mos</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIOINDUCTION</topic><topic>RNA</topic><topic>RNA, Neoplasm - analysis</topic><topic>RODENTS</topic><topic>SARCOMAS</topic><topic>Sialoglycoproteins - analysis</topic><topic>SKELETAL DISEASES</topic><topic>TRANSCRIPTION</topic><topic>Tumors</topic><topic>VERTEBRATES 560152 -- Radiation Effects on Animals-- Animals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>STURM, S. A</creatorcontrib><creatorcontrib>STRAUSS, P. G</creatorcontrib><creatorcontrib>ADOLPH, S</creatorcontrib><creatorcontrib>HAMEISTER, H</creatorcontrib><creatorcontrib>ERFLE, V</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>OSTI.GOV</collection><jtitle>Cancer research (Chicago, Ill.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>STURM, S. A</au><au>STRAUSS, P. G</au><au>ADOLPH, S</au><au>HAMEISTER, H</au><au>ERFLE, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas</atitle><jtitle>Cancer research (Chicago, Ill.)</jtitle><addtitle>Cancer Res</addtitle><date>1990-07-01</date><risdate>1990</risdate><volume>50</volume><issue>13</issue><spage>4146</spage><epage>4153</epage><pages>4146-4153</pages><issn>0008-5472</issn><eissn>1538-7445</eissn><coden>CNREA8</coden><abstract>Fifty-one radiation-induced murine osteosarcomas were investigated for alterations in c-myc gene structure and c-myc expression. Amplification of c-myc was found in 30% of BALB/c tumors and 13% of NMRI tumors. A region of common proviral integration, Mlvi-1, localized on the same region on chromosome 15, was amplified concomitantly. Multiple copies of both loci were localized on double minutes. Three of the tumors with c-myc amplification also showed rearrangements of the c-myc gene region. One of these rearrangements included the 5' and 3'-flanking sequences and the noncoding part of the third exon. Repetitive sequences were found in the 5' region of the c-myc gene, and the 3' flanking region was substituted by sequences normally present in a more distant part of chromosome 15. Increased levels of c-myc transcripts of apparently normal size were found in tumors carrying amplified c-myc sequences. Abnormally high expression of c-myc in some tumors was correlated with an early stage of osteogenic differentiation, suggesting the involvement of the c-myc gene in the control of the osteogenic differentiation of transformed cells.</abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>2141296</pmid><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0008-5472 |
| ispartof | Cancer research (Chicago, Ill.), 1990-07, Vol.50 (13), p.4146-4153 |
| issn | 0008-5472 1538-7445 |
| language | eng |
| recordid | cdi_osti_scitechconnect_6694070 |
| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Animal tumors. Experimental tumors ANIMALS Biological and medical sciences BIOLOGICAL FUNCTIONS Chromosome Mapping CHROMOSOMES DISEASES Experimental bone, joint and muscle tumors EXPERIMENTAL NEOPLASMS Female FUNCTIONS GENE AMPLIFICATION Gene Amplification - genetics Gene Rearrangement - genetics GENE RECOMBINATION GENE RECOMBINATION PROTEINS GENE REGULATION GENES Genomic Library GLYCOPROTEINS KARYOTYPE Karyotyping MAMMALS Medical sciences MICE Mice, Inbred BALB C NEOPLASMS Neoplasms, Radiation-Induced - etiology Neoplasms, Radiation-Induced - genetics NUCLEIC ACIDS NUCLEOPROTEINS ONCOGENES ORGANIC COMPOUNDS Osteopontin Osteosarcoma - etiology Osteosarcoma - genetics OSTEOSARCOMAS PROTEINS Proto-Oncogene Proteins - analysis Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-mos RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIOINDUCTION RNA RNA, Neoplasm - analysis RODENTS SARCOMAS Sialoglycoproteins - analysis SKELETAL DISEASES TRANSCRIPTION Tumors VERTEBRATES 560152 -- Radiation Effects on Animals-- Animals |
| title | Amplification and rearrangement of c-myc in radiation-induced murine osteosarcomas |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T00%3A24%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Amplification%20and%20rearrangement%20of%20c-myc%20in%20radiation-induced%20murine%20osteosarcomas&rft.jtitle=Cancer%20research%20(Chicago,%20Ill.)&rft.au=STURM,%20S.%20A&rft.date=1990-07-01&rft.volume=50&rft.issue=13&rft.spage=4146&rft.epage=4153&rft.pages=4146-4153&rft.issn=0008-5472&rft.eissn=1538-7445&rft.coden=CNREA8&rft_id=info:doi/&rft_dat=%3Cpubmed_osti_%3E2141296%3C/pubmed_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/2141296&rfr_iscdi=true |