Enhanced dna repair and tolerance of DNA damage associated with resistance to cis-diammine-dichloroplatinum (II) after in vitro exposure of a human teratoma cell line to fractionated X-irradiation
In vitro exposure of a human testicular teratoma continuous cell line to fractionated X-irradiation resulted in the expression of resistance to cisplatin. In two independently-derived sublines, designated SUSA-DXR 3 and SUSADXR 10 resulting from treatment with either 13 fractions of 1.5 Gy (dose req...
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| Veröffentlicht in: | International journal of radiation oncology, biology, physics biology, physics, 1990-07, Vol.19 (1), p.75-83 |
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| creator | Hill, Bridget T. Shellard, Sharon A. Hosking, Louise K. Fichtinger-Schepman, Anne Marie J. Bedford, Philip |
| description | In vitro exposure of a human testicular teratoma continuous cell line to fractionated X-irradiation resulted in the expression of resistance to cisplatin. In two independently-derived sublines, designated SUSA-DXR
3 and SUSADXR
10 resulting from treatment with either 13 fractions of 1.5 Gy (dose required to reduce survival by 1 log) or 10 fractions of 3 Gy (dose required to reduce survival by 2 logs) respectivves, the IC
50 values for cisplatin were 2− and 3.1-fold higher than that of the parental cell line. These sublines were cross-resistant to carboplatin (approximately 2-fold) but not to adriamycin and they showed unaltered radiosensitivities. The SUSA-DXR
10 subline expressed some cross-resistance to mitomycin C and melphalan but none to Carmustine (BCNU). Total glutathione content was significantly reduced in both SUSA-DXR
10 and SUSA-DXR
13 cells, but the activities of associated enzymes, including the glutathione S-transferases, peroxidase and reductase were not modified significantly in the resistant sublines. Resistance in the SUSA-DXR
10 subline was associated with significantly decreased
195mcisplatin uptake (p < 0.01), but this was not reflected in a reduced level of drug bound to the DNA. The formation and removal of four platinum DNA adducts were immunochemically quantitated. Immediately following drug treatment there was a higher level of total platination of the DNA in the resistant subline indicative of increased tolerance to DNA damage. After an 18 hr post treatment incubation, there was an indication of some repair capacity in this SUSA-DXR
10 cell line, which was not apparent in the parental cells. Neither the parental nor the SUSA-DXR
10 cell line was proficient in the repair of the major adduct Pt-GG, whereas both lines repaired the monofunctional adduct and the adduct Pt(GMP)
2. SUSA-DXR
10 cells were also able to repair the intrastrand adduct Pt-AG and interstrand crosslinks, unlike the repair deficient parental cells. Higher levels of interstrand crosslinks were characteristic of the SUSA-DXR
10 subline. These observations therefore implicate both enhanced repair and increased tolerance of DNA damage as mechanisms of resistance to cisplatin resulting from
in vitro exposure of a human teratoma cell line to fractionated X-irradiation. |
| doi_str_mv | 10.1016/0360-3016(90)90137-9 |
| format | Article |
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3 and SUSADXR
10 resulting from treatment with either 13 fractions of 1.5 Gy (dose required to reduce survival by 1 log) or 10 fractions of 3 Gy (dose required to reduce survival by 2 logs) respectivves, the IC
50 values for cisplatin were 2− and 3.1-fold higher than that of the parental cell line. These sublines were cross-resistant to carboplatin (approximately 2-fold) but not to adriamycin and they showed unaltered radiosensitivities. The SUSA-DXR
10 subline expressed some cross-resistance to mitomycin C and melphalan but none to Carmustine (BCNU). Total glutathione content was significantly reduced in both SUSA-DXR
10 and SUSA-DXR
13 cells, but the activities of associated enzymes, including the glutathione S-transferases, peroxidase and reductase were not modified significantly in the resistant sublines. Resistance in the SUSA-DXR
10 subline was associated with significantly decreased
195mcisplatin uptake (p < 0.01), but this was not reflected in a reduced level of drug bound to the DNA. The formation and removal of four platinum DNA adducts were immunochemically quantitated. Immediately following drug treatment there was a higher level of total platination of the DNA in the resistant subline indicative of increased tolerance to DNA damage. After an 18 hr post treatment incubation, there was an indication of some repair capacity in this SUSA-DXR
10 cell line, which was not apparent in the parental cells. Neither the parental nor the SUSA-DXR
10 cell line was proficient in the repair of the major adduct Pt-GG, whereas both lines repaired the monofunctional adduct and the adduct Pt(GMP)
2. SUSA-DXR
10 cells were also able to repair the intrastrand adduct Pt-AG and interstrand crosslinks, unlike the repair deficient parental cells. Higher levels of interstrand crosslinks were characteristic of the SUSA-DXR
10 subline. These observations therefore implicate both enhanced repair and increased tolerance of DNA damage as mechanisms of resistance to cisplatin resulting from
in vitro exposure of a human teratoma cell line to fractionated X-irradiation.</description><identifier>ISSN: 0360-3016</identifier><identifier>EISSN: 1879-355X</identifier><identifier>DOI: 10.1016/0360-3016(90)90137-9</identifier><identifier>PMID: 2380098</identifier><identifier>CODEN: IOBPD3</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>ANIMAL CELLS ; ANIMALS ; ANTINEOPLASTIC DRUGS ; Biological and medical sciences ; BIOLOGICAL RECOVERY ; BIOLOGICAL REPAIR ; BODY ; Cisplatin ; Cisplatin - metabolism ; Cisplatin - pharmacokinetics ; Cisplatin - pharmacology ; DISEASES ; DNA - drug effects ; DNA - metabolism ; DNA Adducts ; DNA Damage ; DNA damage and repair ; DNA REPAIR ; DNA Repair - radiation effects ; Dose-Response Relationship, Drug ; DOSE-RESPONSE RELATIONSHIPS ; DOSES ; Drug resistance ; Drug Resistance - radiation effects ; DRUGS ; ENZYMES ; FRACTIONATED IRRADIATION ; Genital system. Mammary gland ; GLUTATHIONE ; Glutathione - metabolism ; Glutathione Peroxidase - metabolism ; Glutathione Reductase - metabolism ; Glutathione Transferase - metabolism ; GONADS ; Humans ; IN VITRO ; IRRADIATION ; Male ; MALE GENITALS ; MAMMALS ; MAN ; Medical sciences ; NEOPLASMS ; ORGANIC COMPOUNDS ; ORGANS ; OXIDOREDUCTASES ; PEPTIDES ; PEROXIDASES ; POLYPEPTIDES ; PRIMATES ; PROTEINS ; Radiation ; RADIATION DOSES ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIOPROTECTIVE SUBSTANCES ; RADIOSENSITIVITY ; Radiotherapy Dosage ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; RECOVERY ; REPAIR ; Teratoma - enzymology ; Teratoma - metabolism ; Teratoma - pathology ; TESTES ; Testicular Neoplasms - enzymology ; Testicular Neoplasms - metabolism ; Testicular Neoplasms - pathology ; Time Factors ; TRANSFERASES ; TUMOR CELLS ; Tumor Cells, Cultured - enzymology ; Tumor Cells, Cultured - radiation effects ; VERTEBRATES 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture</subject><ispartof>International journal of radiation oncology, biology, physics, 1990-07, Vol.19 (1), p.75-83</ispartof><rights>1990</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c328t-18bce0fd3f8427534acf617f94d412841e6d75ed5934eb371f80d3215707ff73</citedby><cites>FETCH-LOGICAL-c328t-18bce0fd3f8427534acf617f94d412841e6d75ed5934eb371f80d3215707ff73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0360-3016(90)90137-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4649867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2380098$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6616416$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Hill, Bridget T.</creatorcontrib><creatorcontrib>Shellard, Sharon A.</creatorcontrib><creatorcontrib>Hosking, Louise K.</creatorcontrib><creatorcontrib>Fichtinger-Schepman, Anne Marie J.</creatorcontrib><creatorcontrib>Bedford, Philip</creatorcontrib><title>Enhanced dna repair and tolerance of DNA damage associated with resistance to cis-diammine-dichloroplatinum (II) after in vitro exposure of a human teratoma cell line to fractionated X-irradiation</title><title>International journal of radiation oncology, biology, physics</title><addtitle>Int J Radiat Oncol Biol Phys</addtitle><description>In vitro exposure of a human testicular teratoma continuous cell line to fractionated X-irradiation resulted in the expression of resistance to cisplatin. In two independently-derived sublines, designated SUSA-DXR
3 and SUSADXR
10 resulting from treatment with either 13 fractions of 1.5 Gy (dose required to reduce survival by 1 log) or 10 fractions of 3 Gy (dose required to reduce survival by 2 logs) respectivves, the IC
50 values for cisplatin were 2− and 3.1-fold higher than that of the parental cell line. These sublines were cross-resistant to carboplatin (approximately 2-fold) but not to adriamycin and they showed unaltered radiosensitivities. The SUSA-DXR
10 subline expressed some cross-resistance to mitomycin C and melphalan but none to Carmustine (BCNU). Total glutathione content was significantly reduced in both SUSA-DXR
10 and SUSA-DXR
13 cells, but the activities of associated enzymes, including the glutathione S-transferases, peroxidase and reductase were not modified significantly in the resistant sublines. Resistance in the SUSA-DXR
10 subline was associated with significantly decreased
195mcisplatin uptake (p < 0.01), but this was not reflected in a reduced level of drug bound to the DNA. The formation and removal of four platinum DNA adducts were immunochemically quantitated. Immediately following drug treatment there was a higher level of total platination of the DNA in the resistant subline indicative of increased tolerance to DNA damage. After an 18 hr post treatment incubation, there was an indication of some repair capacity in this SUSA-DXR
10 cell line, which was not apparent in the parental cells. Neither the parental nor the SUSA-DXR
10 cell line was proficient in the repair of the major adduct Pt-GG, whereas both lines repaired the monofunctional adduct and the adduct Pt(GMP)
2. SUSA-DXR
10 cells were also able to repair the intrastrand adduct Pt-AG and interstrand crosslinks, unlike the repair deficient parental cells. Higher levels of interstrand crosslinks were characteristic of the SUSA-DXR
10 subline. These observations therefore implicate both enhanced repair and increased tolerance of DNA damage as mechanisms of resistance to cisplatin resulting from
in vitro exposure of a human teratoma cell line to fractionated X-irradiation.</description><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>ANTINEOPLASTIC DRUGS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL RECOVERY</subject><subject>BIOLOGICAL REPAIR</subject><subject>BODY</subject><subject>Cisplatin</subject><subject>Cisplatin - metabolism</subject><subject>Cisplatin - pharmacokinetics</subject><subject>Cisplatin - pharmacology</subject><subject>DISEASES</subject><subject>DNA - drug effects</subject><subject>DNA - metabolism</subject><subject>DNA Adducts</subject><subject>DNA Damage</subject><subject>DNA damage and repair</subject><subject>DNA REPAIR</subject><subject>DNA Repair - radiation effects</subject><subject>Dose-Response Relationship, Drug</subject><subject>DOSE-RESPONSE RELATIONSHIPS</subject><subject>DOSES</subject><subject>Drug resistance</subject><subject>Drug Resistance - radiation effects</subject><subject>DRUGS</subject><subject>ENZYMES</subject><subject>FRACTIONATED IRRADIATION</subject><subject>Genital system. Mammary gland</subject><subject>GLUTATHIONE</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Glutathione Reductase - metabolism</subject><subject>Glutathione Transferase - metabolism</subject><subject>GONADS</subject><subject>Humans</subject><subject>IN VITRO</subject><subject>IRRADIATION</subject><subject>Male</subject><subject>MALE GENITALS</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>Medical sciences</subject><subject>NEOPLASMS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>OXIDOREDUCTASES</subject><subject>PEPTIDES</subject><subject>PEROXIDASES</subject><subject>POLYPEPTIDES</subject><subject>PRIMATES</subject><subject>PROTEINS</subject><subject>Radiation</subject><subject>RADIATION DOSES</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIOPROTECTIVE SUBSTANCES</subject><subject>RADIOSENSITIVITY</subject><subject>Radiotherapy Dosage</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>RECOVERY</subject><subject>REPAIR</subject><subject>Teratoma - enzymology</subject><subject>Teratoma - metabolism</subject><subject>Teratoma - pathology</subject><subject>TESTES</subject><subject>Testicular Neoplasms - enzymology</subject><subject>Testicular Neoplasms - metabolism</subject><subject>Testicular Neoplasms - pathology</subject><subject>Time Factors</subject><subject>TRANSFERASES</subject><subject>TUMOR CELLS</subject><subject>Tumor Cells, Cultured - enzymology</subject><subject>Tumor Cells, Cultured - radiation effects</subject><subject>VERTEBRATES 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture</subject><issn>0360-3016</issn><issn>1879-355X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxiMEKtvCG4BkIQ7tIWDHjh1fkKq2wEoVXHroLZr1H2KU2CvbW-D9eDDs3dUeOXnk-X0zn-ZrmjcEfyCY8I-YctzSUl1KfCUxoaKVz5oVGYRsad8_Pm9WJ-Rlc57ST4wxIYKdNWcdHTCWw6r5e-cn8MpopD2gaLbgIgKvUQ6zibWDgkW3366RhgV-GAQpBeUgF8Uvl6ciSS7lPZgDUi612sGyOG9KoaY5xLCdITu_W9Dlen2FwGYTkfPoyeUYkPm9DWkX92sATbsFPCoA5LAAUmae0Vxm1dk2gsou-P3ux9bFCGVV_XnVvLAwJ_P6-F40D5_vHm6-tvffv6xvru9bRbsht2TYKIOtpnZgnegpA2U5EVYyzUg3MGK4Fr3RvaTMbKggdsCadqQXWFgr6EXz7jA2pOzGpFw2alLBe6PyyDnhjPACsQOkYkgpGjtuo1sg_hkJHmtuYw1lrKGMEo_73EZZZG8Psu1usxh9Eh2DKv33xz4kBbOt0bh0whhncuDV4qcDZsoZnpyJ1aap-bpYXerg_u_jH_eutrg</recordid><startdate>199007</startdate><enddate>199007</enddate><creator>Hill, Bridget T.</creator><creator>Shellard, Sharon A.</creator><creator>Hosking, Louise K.</creator><creator>Fichtinger-Schepman, Anne Marie J.</creator><creator>Bedford, Philip</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>199007</creationdate><title>Enhanced dna repair and tolerance of DNA damage associated with resistance to cis-diammine-dichloroplatinum (II) after in vitro exposure of a human teratoma cell line to fractionated X-irradiation</title><author>Hill, Bridget T. ; Shellard, Sharon A. ; Hosking, Louise K. ; Fichtinger-Schepman, Anne Marie J. ; Bedford, Philip</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-18bce0fd3f8427534acf617f94d412841e6d75ed5934eb371f80d3215707ff73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>ANTINEOPLASTIC DRUGS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>BODY</topic><topic>Cisplatin</topic><topic>Cisplatin - metabolism</topic><topic>Cisplatin - pharmacokinetics</topic><topic>Cisplatin - pharmacology</topic><topic>DISEASES</topic><topic>DNA - drug effects</topic><topic>DNA - metabolism</topic><topic>DNA Adducts</topic><topic>DNA Damage</topic><topic>DNA damage and repair</topic><topic>DNA REPAIR</topic><topic>DNA Repair - radiation effects</topic><topic>Dose-Response Relationship, Drug</topic><topic>DOSE-RESPONSE RELATIONSHIPS</topic><topic>DOSES</topic><topic>Drug resistance</topic><topic>Drug Resistance - radiation effects</topic><topic>DRUGS</topic><topic>ENZYMES</topic><topic>FRACTIONATED IRRADIATION</topic><topic>Genital system. Mammary gland</topic><topic>GLUTATHIONE</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Glutathione Reductase - metabolism</topic><topic>Glutathione Transferase - metabolism</topic><topic>GONADS</topic><topic>Humans</topic><topic>IN VITRO</topic><topic>IRRADIATION</topic><topic>Male</topic><topic>MALE GENITALS</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>Medical sciences</topic><topic>NEOPLASMS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>OXIDOREDUCTASES</topic><topic>PEPTIDES</topic><topic>PEROXIDASES</topic><topic>POLYPEPTIDES</topic><topic>PRIMATES</topic><topic>PROTEINS</topic><topic>Radiation</topic><topic>RADIATION DOSES</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIOPROTECTIVE SUBSTANCES</topic><topic>RADIOSENSITIVITY</topic><topic>Radiotherapy Dosage</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>RECOVERY</topic><topic>REPAIR</topic><topic>Teratoma - enzymology</topic><topic>Teratoma - metabolism</topic><topic>Teratoma - pathology</topic><topic>TESTES</topic><topic>Testicular Neoplasms - enzymology</topic><topic>Testicular Neoplasms - metabolism</topic><topic>Testicular Neoplasms - pathology</topic><topic>Time Factors</topic><topic>TRANSFERASES</topic><topic>TUMOR CELLS</topic><topic>Tumor Cells, Cultured - enzymology</topic><topic>Tumor Cells, Cultured - radiation effects</topic><topic>VERTEBRATES 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hill, Bridget T.</creatorcontrib><creatorcontrib>Shellard, Sharon A.</creatorcontrib><creatorcontrib>Hosking, Louise K.</creatorcontrib><creatorcontrib>Fichtinger-Schepman, Anne Marie J.</creatorcontrib><creatorcontrib>Bedford, Philip</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>International journal of radiation oncology, biology, physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hill, Bridget T.</au><au>Shellard, Sharon A.</au><au>Hosking, Louise K.</au><au>Fichtinger-Schepman, Anne Marie J.</au><au>Bedford, Philip</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced dna repair and tolerance of DNA damage associated with resistance to cis-diammine-dichloroplatinum (II) after in vitro exposure of a human teratoma cell line to fractionated X-irradiation</atitle><jtitle>International journal of radiation oncology, biology, physics</jtitle><addtitle>Int J Radiat Oncol Biol Phys</addtitle><date>1990-07</date><risdate>1990</risdate><volume>19</volume><issue>1</issue><spage>75</spage><epage>83</epage><pages>75-83</pages><issn>0360-3016</issn><eissn>1879-355X</eissn><coden>IOBPD3</coden><abstract>In vitro exposure of a human testicular teratoma continuous cell line to fractionated X-irradiation resulted in the expression of resistance to cisplatin. In two independently-derived sublines, designated SUSA-DXR
3 and SUSADXR
10 resulting from treatment with either 13 fractions of 1.5 Gy (dose required to reduce survival by 1 log) or 10 fractions of 3 Gy (dose required to reduce survival by 2 logs) respectivves, the IC
50 values for cisplatin were 2− and 3.1-fold higher than that of the parental cell line. These sublines were cross-resistant to carboplatin (approximately 2-fold) but not to adriamycin and they showed unaltered radiosensitivities. The SUSA-DXR
10 subline expressed some cross-resistance to mitomycin C and melphalan but none to Carmustine (BCNU). Total glutathione content was significantly reduced in both SUSA-DXR
10 and SUSA-DXR
13 cells, but the activities of associated enzymes, including the glutathione S-transferases, peroxidase and reductase were not modified significantly in the resistant sublines. Resistance in the SUSA-DXR
10 subline was associated with significantly decreased
195mcisplatin uptake (p < 0.01), but this was not reflected in a reduced level of drug bound to the DNA. The formation and removal of four platinum DNA adducts were immunochemically quantitated. Immediately following drug treatment there was a higher level of total platination of the DNA in the resistant subline indicative of increased tolerance to DNA damage. After an 18 hr post treatment incubation, there was an indication of some repair capacity in this SUSA-DXR
10 cell line, which was not apparent in the parental cells. Neither the parental nor the SUSA-DXR
10 cell line was proficient in the repair of the major adduct Pt-GG, whereas both lines repaired the monofunctional adduct and the adduct Pt(GMP)
2. SUSA-DXR
10 cells were also able to repair the intrastrand adduct Pt-AG and interstrand crosslinks, unlike the repair deficient parental cells. Higher levels of interstrand crosslinks were characteristic of the SUSA-DXR
10 subline. These observations therefore implicate both enhanced repair and increased tolerance of DNA damage as mechanisms of resistance to cisplatin resulting from
in vitro exposure of a human teratoma cell line to fractionated X-irradiation.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2380098</pmid><doi>10.1016/0360-3016(90)90137-9</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0360-3016 |
| ispartof | International journal of radiation oncology, biology, physics, 1990-07, Vol.19 (1), p.75-83 |
| issn | 0360-3016 1879-355X |
| language | eng |
| recordid | cdi_osti_scitechconnect_6616416 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | ANIMAL CELLS ANIMALS ANTINEOPLASTIC DRUGS Biological and medical sciences BIOLOGICAL RECOVERY BIOLOGICAL REPAIR BODY Cisplatin Cisplatin - metabolism Cisplatin - pharmacokinetics Cisplatin - pharmacology DISEASES DNA - drug effects DNA - metabolism DNA Adducts DNA Damage DNA damage and repair DNA REPAIR DNA Repair - radiation effects Dose-Response Relationship, Drug DOSE-RESPONSE RELATIONSHIPS DOSES Drug resistance Drug Resistance - radiation effects DRUGS ENZYMES FRACTIONATED IRRADIATION Genital system. Mammary gland GLUTATHIONE Glutathione - metabolism Glutathione Peroxidase - metabolism Glutathione Reductase - metabolism Glutathione Transferase - metabolism GONADS Humans IN VITRO IRRADIATION Male MALE GENITALS MAMMALS MAN Medical sciences NEOPLASMS ORGANIC COMPOUNDS ORGANS OXIDOREDUCTASES PEPTIDES PEROXIDASES POLYPEPTIDES PRIMATES PROTEINS Radiation RADIATION DOSES RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIOPROTECTIVE SUBSTANCES RADIOSENSITIVITY Radiotherapy Dosage Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) RECOVERY REPAIR Teratoma - enzymology Teratoma - metabolism Teratoma - pathology TESTES Testicular Neoplasms - enzymology Testicular Neoplasms - metabolism Testicular Neoplasms - pathology Time Factors TRANSFERASES TUMOR CELLS Tumor Cells, Cultured - enzymology Tumor Cells, Cultured - radiation effects VERTEBRATES 560120 -- Radiation Effects on Biochemicals, Cells, & Tissue Culture |
| title | Enhanced dna repair and tolerance of DNA damage associated with resistance to cis-diammine-dichloroplatinum (II) after in vitro exposure of a human teratoma cell line to fractionated X-irradiation |
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