Relationship of Membrane-Bound Sulfhydryl Groups to Vitamin D-Stimulated Uptake of [75Se]Selenite by the Brush Border Membrane Vesicles from Chick Duodenum
The uptake of selenite by purified brush border membrane vesicles isolated from duodena of rachitic or vitamin D-treated chicks was studied by using radioactive selenite and a rapid filtration technique. Cholecalciferol treatment (500 IU at 72 h) significantly enhanced selenite uptake, a response th...
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| Veröffentlicht in: | The Journal of nutrition 1990-08, Vol.120 (8), p.882-888 |
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| description | The uptake of selenite by purified brush border membrane vesicles isolated from duodena of rachitic or vitamin D-treated chicks was studied by using radioactive selenite and a rapid filtration technique. Cholecalciferol treatment (500 IU at 72 h) significantly enhanced selenite uptake, a response that decreased when the vesicles were stored at room temperature for 2.5 h prior to the uptake measurement. Preincubation of the vesicles in 1.0 mmol/L H2O2 reduced [75Se]selenite uptake, indicating the involvement of oxidizable groups in the uptake reaction. Iodoacetic acid (IAA), a sulfhydryl-blocking reagent, at 1–2 mmol/L concentration eliminated the difference in selenite uptake due to cholecalciferol and had no effect on vesicles from rachitic animals. A higher concentration of IAA (10 mmol/L) enhanced selenite uptake manyfold and increased the absolute difference due to cholecalciferol treatment. Single intravenous doses of 100 IU cholecalciferol, 100 IU ergocalciferol, or 0.1 µg 1,25-dihydroxycholecalciferol also stimulated selenite uptake, suggesting a general response to vitamin D compounds. Normal animals given a single dose of 1,25-dihydroxycholecalciferol 12 h prior to killing also responded. Treatments that enhanced the uptake of [75Se]selenite also increased the amount of membrane-bound sulfhydryl groups, suggesting the involvement of membrane-bound sulfhydryl groups in the vitamin D response. A significant increase in selenite uptake by intravenous 1,25-dihydroxycholecalciferol occurred within 10 min. This rapid effect provides a new tool to probe early biochemical effects of vitamin D on intestinal epithelium. |
| doi_str_mv | 10.1093/jn/120.8.882 |
| format | Article |
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Cholecalciferol treatment (500 IU at 72 h) significantly enhanced selenite uptake, a response that decreased when the vesicles were stored at room temperature for 2.5 h prior to the uptake measurement. Preincubation of the vesicles in 1.0 mmol/L H2O2 reduced [75Se]selenite uptake, indicating the involvement of oxidizable groups in the uptake reaction. Iodoacetic acid (IAA), a sulfhydryl-blocking reagent, at 1–2 mmol/L concentration eliminated the difference in selenite uptake due to cholecalciferol and had no effect on vesicles from rachitic animals. A higher concentration of IAA (10 mmol/L) enhanced selenite uptake manyfold and increased the absolute difference due to cholecalciferol treatment. Single intravenous doses of 100 IU cholecalciferol, 100 IU ergocalciferol, or 0.1 µg 1,25-dihydroxycholecalciferol also stimulated selenite uptake, suggesting a general response to vitamin D compounds. Normal animals given a single dose of 1,25-dihydroxycholecalciferol 12 h prior to killing also responded. Treatments that enhanced the uptake of [75Se]selenite also increased the amount of membrane-bound sulfhydryl groups, suggesting the involvement of membrane-bound sulfhydryl groups in the vitamin D response. A significant increase in selenite uptake by intravenous 1,25-dihydroxycholecalciferol occurred within 10 min. This rapid effect provides a new tool to probe early biochemical effects of vitamin D on intestinal epithelium.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/120.8.882</identifier><identifier>PMID: 2166146</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>550501 -- Metabolism-- Tracer Techniques ; ABSORCION DE SUBSTANCIAS NUTRITIVAS ; ABSORPTION DE SUBSTANCES NUTRITIVES ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BIOCHEMICAL REACTION KINETICS ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; BIRDS ; BODY ; brush border membrane vesicles ; Calcitriol - pharmacology ; CELL CONSTITUENTS ; CELL MEMBRANES ; CHICKENS ; chicks ; CHOLECALCIFEROL ; Cholecalciferol - pharmacology ; DAYS LIVING RADIOISOTOPES ; DIGESTIVE SYSTEM ; Duodenum - drug effects ; Duodenum - metabolism ; ELECTRON CAPTURE RADIOISOTOPES ; ELEMENTS ; Ergocalciferols - pharmacology ; EVEN-ODD NUCLEI ; FOWL ; Fundamental and applied biological sciences. Psychology ; GASTROINTESTINAL TRACT ; Hydrogen Peroxide - pharmacology ; INTERMEDIATE MASS NUCLEI ; INTESTIN ; Intestine. Mesentery ; INTESTINES ; INTESTINOS ; Iodoacetates - pharmacology ; Iodoacetic Acid ; ISOTOPE APPLICATIONS ; ISOTOPES ; KINETICS ; Male ; MEMBRANE TRANSPORT ; MEMBRANES ; METABOLISM ; Microvilli - drug effects ; Microvilli - metabolism ; NUCLEI ; ORGANS ; POLLITO ; POUSSIN ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIOISOTOPES ; REACTION KINETICS ; REAGENTS ; Rickets - metabolism ; SELENIO ; Selenious Acid ; selenite ; SELENIUM ; Selenium - metabolism ; SELENIUM 75 ; SELENIUM ISOTOPES ; Selenium Radioisotopes ; SEMIMETALS ; SMALL INTESTINE ; Sulfhydryl Compounds - metabolism ; sulfhydryl groups ; THIOL ; TIOLES ; TRACER TECHNIQUES ; VERTEBRATES ; Vertebrates: digestive system ; VITAMIN D ; Vitamin D - pharmacology ; VITAMINA D ; VITAMINE D ; VITAMINS 560300 -- Chemicals Metabolism & Toxicology</subject><ispartof>The Journal of nutrition, 1990-08, Vol.120 (8), p.882-888</ispartof><rights>1990 American Society for Nutrition.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-d39f99e596e697eb59b621fecb8d02e9952117f6541812c386b2afa91d688c403</citedby><cites>FETCH-LOGICAL-c447t-d39f99e596e697eb59b621fecb8d02e9952117f6541812c386b2afa91d688c403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,782,786,887,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19818438$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2166146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6612600$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Mykkanen, Hannu M.</creatorcontrib><creatorcontrib>Wasserman, Robert H.</creatorcontrib><title>Relationship of Membrane-Bound Sulfhydryl Groups to Vitamin D-Stimulated Uptake of [75Se]Selenite by the Brush Border Membrane Vesicles from Chick Duodenum</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>The uptake of selenite by purified brush border membrane vesicles isolated from duodena of rachitic or vitamin D-treated chicks was studied by using radioactive selenite and a rapid filtration technique. Cholecalciferol treatment (500 IU at 72 h) significantly enhanced selenite uptake, a response that decreased when the vesicles were stored at room temperature for 2.5 h prior to the uptake measurement. Preincubation of the vesicles in 1.0 mmol/L H2O2 reduced [75Se]selenite uptake, indicating the involvement of oxidizable groups in the uptake reaction. Iodoacetic acid (IAA), a sulfhydryl-blocking reagent, at 1–2 mmol/L concentration eliminated the difference in selenite uptake due to cholecalciferol and had no effect on vesicles from rachitic animals. A higher concentration of IAA (10 mmol/L) enhanced selenite uptake manyfold and increased the absolute difference due to cholecalciferol treatment. Single intravenous doses of 100 IU cholecalciferol, 100 IU ergocalciferol, or 0.1 µg 1,25-dihydroxycholecalciferol also stimulated selenite uptake, suggesting a general response to vitamin D compounds. Normal animals given a single dose of 1,25-dihydroxycholecalciferol 12 h prior to killing also responded. Treatments that enhanced the uptake of [75Se]selenite also increased the amount of membrane-bound sulfhydryl groups, suggesting the involvement of membrane-bound sulfhydryl groups in the vitamin D response. A significant increase in selenite uptake by intravenous 1,25-dihydroxycholecalciferol occurred within 10 min. This rapid effect provides a new tool to probe early biochemical effects of vitamin D on intestinal epithelium.</description><subject>550501 -- Metabolism-- Tracer Techniques</subject><subject>ABSORCION DE SUBSTANCIAS NUTRITIVAS</subject><subject>ABSORPTION DE SUBSTANCES NUTRITIVES</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BIOCHEMICAL REACTION KINETICS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIRDS</subject><subject>BODY</subject><subject>brush border membrane vesicles</subject><subject>Calcitriol - pharmacology</subject><subject>CELL CONSTITUENTS</subject><subject>CELL MEMBRANES</subject><subject>CHICKENS</subject><subject>chicks</subject><subject>CHOLECALCIFEROL</subject><subject>Cholecalciferol - pharmacology</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>DIGESTIVE SYSTEM</subject><subject>Duodenum - drug effects</subject><subject>Duodenum - metabolism</subject><subject>ELECTRON CAPTURE RADIOISOTOPES</subject><subject>ELEMENTS</subject><subject>Ergocalciferols - pharmacology</subject><subject>EVEN-ODD NUCLEI</subject><subject>FOWL</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GASTROINTESTINAL TRACT</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>INTESTIN</subject><subject>Intestine. Mesentery</subject><subject>INTESTINES</subject><subject>INTESTINOS</subject><subject>Iodoacetates - pharmacology</subject><subject>Iodoacetic Acid</subject><subject>ISOTOPE APPLICATIONS</subject><subject>ISOTOPES</subject><subject>KINETICS</subject><subject>Male</subject><subject>MEMBRANE TRANSPORT</subject><subject>MEMBRANES</subject><subject>METABOLISM</subject><subject>Microvilli - drug effects</subject><subject>Microvilli - metabolism</subject><subject>NUCLEI</subject><subject>ORGANS</subject><subject>POLLITO</subject><subject>POUSSIN</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIOISOTOPES</subject><subject>REACTION KINETICS</subject><subject>REAGENTS</subject><subject>Rickets - metabolism</subject><subject>SELENIO</subject><subject>Selenious Acid</subject><subject>selenite</subject><subject>SELENIUM</subject><subject>Selenium - metabolism</subject><subject>SELENIUM 75</subject><subject>SELENIUM ISOTOPES</subject><subject>Selenium Radioisotopes</subject><subject>SEMIMETALS</subject><subject>SMALL INTESTINE</subject><subject>Sulfhydryl Compounds - metabolism</subject><subject>sulfhydryl groups</subject><subject>THIOL</subject><subject>TIOLES</subject><subject>TRACER TECHNIQUES</subject><subject>VERTEBRATES</subject><subject>Vertebrates: digestive system</subject><subject>VITAMIN D</subject><subject>Vitamin D - pharmacology</subject><subject>VITAMINA D</subject><subject>VITAMINE D</subject><subject>VITAMINS 560300 -- Chemicals Metabolism & Toxicology</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkVGLEzEUhQdR1u7qm0-CEAR9crpJZpomj7a7rsKKYO2-iIRM5sZJdyapSUbob_HPmqFlffEpcO_HyT3nFMULgucEi-py5y4JxXM-55w-KmZkUZOSEYwfFzOMKS0rwtjT4jzGHcaY1IKfFWc0z0jNZsWfr9CrZL2Lnd0jb9BnGJqgHJQrP7oWbcbedIc2HHp0E_y4jyh5dGeTGqxDV-Um2WHMAtCi7T6pe5gkvi8XG_ixgR6cTYCaA0odoFUYY4dWPrQQHn5BdxCt7iEiE_yA1p3V9-hq9C24cXhWPDGqj_D89F4U2w_X39Yfy9svN5_W729LXdfLVLaVMELAQjBgYgnNQjSMEgO64S2mIMSCErI0LOfCCdUVZw1VRgnSMs51jauL4vVR18dkZdT5aN1p7xzoJHNOlOEJenuE9sH_GiEmOdiooe-zCz9GuRSC1hRXGXx3BHXwMQYwch_soMJBEiynwuTOyVyY5DIXlvFXJ92xGaB9gE8N5f2b015FrXqTU9M2_tMUnPC64pl7eeSM8lL9DJnZbgSuBamm49lxCTnG3xbC5BKchtaGyWTr7f-v-wvQSLj0</recordid><startdate>19900801</startdate><enddate>19900801</enddate><creator>Mykkanen, Hannu M.</creator><creator>Wasserman, Robert H.</creator><general>Elsevier Inc</general><general>American Society for Nutritional Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19900801</creationdate><title>Relationship of Membrane-Bound Sulfhydryl Groups to Vitamin D-Stimulated Uptake of [75Se]Selenite by the Brush Border Membrane Vesicles from Chick Duodenum</title><author>Mykkanen, Hannu M. ; Wasserman, Robert H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-d39f99e596e697eb59b621fecb8d02e9952117f6541812c386b2afa91d688c403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>550501 -- Metabolism-- Tracer Techniques</topic><topic>ABSORCION DE SUBSTANCIAS NUTRITIVAS</topic><topic>ABSORPTION DE SUBSTANCES NUTRITIVES</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BIOCHEMICAL REACTION KINETICS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIRDS</topic><topic>BODY</topic><topic>brush border membrane vesicles</topic><topic>Calcitriol - pharmacology</topic><topic>CELL CONSTITUENTS</topic><topic>CELL MEMBRANES</topic><topic>CHICKENS</topic><topic>chicks</topic><topic>CHOLECALCIFEROL</topic><topic>Cholecalciferol - pharmacology</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>DIGESTIVE SYSTEM</topic><topic>Duodenum - drug effects</topic><topic>Duodenum - metabolism</topic><topic>ELECTRON CAPTURE RADIOISOTOPES</topic><topic>ELEMENTS</topic><topic>Ergocalciferols - pharmacology</topic><topic>EVEN-ODD NUCLEI</topic><topic>FOWL</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GASTROINTESTINAL TRACT</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>INTESTIN</topic><topic>Intestine. Mesentery</topic><topic>INTESTINES</topic><topic>INTESTINOS</topic><topic>Iodoacetates - pharmacology</topic><topic>Iodoacetic Acid</topic><topic>ISOTOPE APPLICATIONS</topic><topic>ISOTOPES</topic><topic>KINETICS</topic><topic>Male</topic><topic>MEMBRANE TRANSPORT</topic><topic>MEMBRANES</topic><topic>METABOLISM</topic><topic>Microvilli - drug effects</topic><topic>Microvilli - metabolism</topic><topic>NUCLEI</topic><topic>ORGANS</topic><topic>POLLITO</topic><topic>POUSSIN</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIOISOTOPES</topic><topic>REACTION KINETICS</topic><topic>REAGENTS</topic><topic>Rickets - metabolism</topic><topic>SELENIO</topic><topic>Selenious Acid</topic><topic>selenite</topic><topic>SELENIUM</topic><topic>Selenium - metabolism</topic><topic>SELENIUM 75</topic><topic>SELENIUM ISOTOPES</topic><topic>Selenium Radioisotopes</topic><topic>SEMIMETALS</topic><topic>SMALL INTESTINE</topic><topic>Sulfhydryl Compounds - metabolism</topic><topic>sulfhydryl groups</topic><topic>THIOL</topic><topic>TIOLES</topic><topic>TRACER TECHNIQUES</topic><topic>VERTEBRATES</topic><topic>Vertebrates: digestive system</topic><topic>VITAMIN D</topic><topic>Vitamin D - pharmacology</topic><topic>VITAMINA D</topic><topic>VITAMINE D</topic><topic>VITAMINS 560300 -- Chemicals Metabolism & Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mykkanen, Hannu M.</creatorcontrib><creatorcontrib>Wasserman, Robert H.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mykkanen, Hannu M.</au><au>Wasserman, Robert H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationship of Membrane-Bound Sulfhydryl Groups to Vitamin D-Stimulated Uptake of [75Se]Selenite by the Brush Border Membrane Vesicles from Chick Duodenum</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1990-08-01</date><risdate>1990</risdate><volume>120</volume><issue>8</issue><spage>882</spage><epage>888</epage><pages>882-888</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>The uptake of selenite by purified brush border membrane vesicles isolated from duodena of rachitic or vitamin D-treated chicks was studied by using radioactive selenite and a rapid filtration technique. Cholecalciferol treatment (500 IU at 72 h) significantly enhanced selenite uptake, a response that decreased when the vesicles were stored at room temperature for 2.5 h prior to the uptake measurement. Preincubation of the vesicles in 1.0 mmol/L H2O2 reduced [75Se]selenite uptake, indicating the involvement of oxidizable groups in the uptake reaction. Iodoacetic acid (IAA), a sulfhydryl-blocking reagent, at 1–2 mmol/L concentration eliminated the difference in selenite uptake due to cholecalciferol and had no effect on vesicles from rachitic animals. A higher concentration of IAA (10 mmol/L) enhanced selenite uptake manyfold and increased the absolute difference due to cholecalciferol treatment. Single intravenous doses of 100 IU cholecalciferol, 100 IU ergocalciferol, or 0.1 µg 1,25-dihydroxycholecalciferol also stimulated selenite uptake, suggesting a general response to vitamin D compounds. Normal animals given a single dose of 1,25-dihydroxycholecalciferol 12 h prior to killing also responded. Treatments that enhanced the uptake of [75Se]selenite also increased the amount of membrane-bound sulfhydryl groups, suggesting the involvement of membrane-bound sulfhydryl groups in the vitamin D response. A significant increase in selenite uptake by intravenous 1,25-dihydroxycholecalciferol occurred within 10 min. This rapid effect provides a new tool to probe early biochemical effects of vitamin D on intestinal epithelium.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2166146</pmid><doi>10.1093/jn/120.8.882</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | The Journal of nutrition, 1990-08, Vol.120 (8), p.882-888 |
| issn | 0022-3166 1541-6100 |
| language | eng |
| recordid | cdi_osti_scitechconnect_6612600 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | 550501 -- Metabolism-- Tracer Techniques ABSORCION DE SUBSTANCIAS NUTRITIVAS ABSORPTION DE SUBSTANCES NUTRITIVES ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BIOCHEMICAL REACTION KINETICS Biological and medical sciences BIOLOGICAL EFFECTS BIRDS BODY brush border membrane vesicles Calcitriol - pharmacology CELL CONSTITUENTS CELL MEMBRANES CHICKENS chicks CHOLECALCIFEROL Cholecalciferol - pharmacology DAYS LIVING RADIOISOTOPES DIGESTIVE SYSTEM Duodenum - drug effects Duodenum - metabolism ELECTRON CAPTURE RADIOISOTOPES ELEMENTS Ergocalciferols - pharmacology EVEN-ODD NUCLEI FOWL Fundamental and applied biological sciences. Psychology GASTROINTESTINAL TRACT Hydrogen Peroxide - pharmacology INTERMEDIATE MASS NUCLEI INTESTIN Intestine. Mesentery INTESTINES INTESTINOS Iodoacetates - pharmacology Iodoacetic Acid ISOTOPE APPLICATIONS ISOTOPES KINETICS Male MEMBRANE TRANSPORT MEMBRANES METABOLISM Microvilli - drug effects Microvilli - metabolism NUCLEI ORGANS POLLITO POUSSIN RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIOISOTOPES REACTION KINETICS REAGENTS Rickets - metabolism SELENIO Selenious Acid selenite SELENIUM Selenium - metabolism SELENIUM 75 SELENIUM ISOTOPES Selenium Radioisotopes SEMIMETALS SMALL INTESTINE Sulfhydryl Compounds - metabolism sulfhydryl groups THIOL TIOLES TRACER TECHNIQUES VERTEBRATES Vertebrates: digestive system VITAMIN D Vitamin D - pharmacology VITAMINA D VITAMINE D VITAMINS 560300 -- Chemicals Metabolism & Toxicology |
| title | Relationship of Membrane-Bound Sulfhydryl Groups to Vitamin D-Stimulated Uptake of [75Se]Selenite by the Brush Border Membrane Vesicles from Chick Duodenum |
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