Modification of MCDB 110 medium to support prolonged growth and consistent high cloning efficiency of diploid human fibroblasts

In preparation for studies on the growth factor requirements of normal and transformed human fibroblasts, we have developed a serum-free medium that supports vigorous longterm serial subculture of diploid human fibroblasts and allows them to form large-sized colonies with high efficiency (40 to 60%)...

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Veröffentlicht in:	Experimental cell research 1987-10, Vol.172 (2), p.318-328
Hauptverfasser:	Ryan, P.Ann, Maher, Veronica M., McCormick, J.Justin
Format:	Artikel
Sprache:	eng
Schlagworte:	550300 - Cytology ANIMAL CELLS ANIMALS BASIC BIOLOGICAL SCIENCES Biological and medical sciences BIOLOGICAL EFFECTS Biotechnology Cell Adhesion - drug effects CELL CULTURES Cell cultures. Hybridization. Fusion Cell Division - drug effects Clone Cells Colony-Forming Units Assay CONNECTIVE TISSUE CELLS CULTURE MEDIA Culture Media - analysis Culture Media - pharmacology CYTOLOGICAL TECHNIQUES Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures FIBROBLASTS Fibroblasts - cytology Fibroblasts - drug effects Fundamental and applied biological sciences. Psychology GROWTH GROWTH FACTORS Growth Substances - analysis Humans MAMMALS MAN Methods. Procedures. Technologies MITOGENS Molecular and cellular biology ORGANIC COMPOUNDS PRIMATES PROTEINS SOMATIC CELLS VERTEBRATES
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