Primary Sequence Information from Intact Proteins by Electrospray Ionization Tandem Mass Spectrometry

Tandem mass spectrometry has been used to obtain information related to portions of the primary sequence for an intact protein, bovine ribonuclease A. Multiply charged molecular ions, generated by electrospray ionization, were collisionally dissociated at low energies in a triple quadrupole mass spe...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1990-04, Vol.248 (4952), p.201-204
Hauptverfasser:	Loo, Joseph A., Edmonds, Charles G., Smith, Richard D.
Format:	Artikel
Sprache:	eng
Schlagworte:	400201 - Chemical & Physicochemical Properties 550300 - Cytology Amino Acid Sequence Analysis ANIMALS BASIC BIOLOGICAL SCIENCES Biochemistry Biological and medical sciences CATTLE Chemical bonding Chemistry DATA Disulfides DOMESTIC ANIMALS ENZYMES ESTERASES EXPERIMENTAL DATA Fundamental and applied biological sciences. Psychology HYDROLASES INFORMATION INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY IONIZATION Ions MAMMALS MASS SPECTRA Mass spectrometers Mass spectrometry Mass Spectrometry - methods Mass spectroscopy Molecular biophysics Molecular ions MOLECULAR STRUCTURE Molecules NUMERICAL DATA Oxidation-Reduction PHOSPHODIESTERASES Proteins Quadrupoles Ribonuclease Ribonuclease, Pancreatic RNA-ASE RUMINANTS SPECTRA Spectroscopy : techniques and spectras VERTEBRATES
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