Protein kinase A phosphorylates retinal phosducin on serine 73 in situ

Photoreceptors of vertebrate retinas contain a 33,000-dalton phosphoprotein, phosducin, which complexes with the beta, gamma subunits of the photoreceptor G-protein (guanine nucleotide-binding protein), transducin. In situ, the retinal content of phosphorylated phosducin is modulated by light in con...

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Veröffentlicht in:	The Journal of biological chemistry 1990-09, Vol.265 (26), p.15860-15866
Hauptverfasser:	Lee, R H, Brown, B M, Lolley, R N
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques Adenosine Triphosphate - metabolism AMINO ACID SEQUENCE ANIMALS ANTIBODIES Antibodies, Monoclonal ATP BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOASSAY BIOCHEMICAL REACTION KINETICS Blotting, Western BODY BODY AREAS CATTAILS CATTLE CHEMICAL REACTIONS CHROMATOGRAPHY Chromatography, High Pressure Liquid Cyanogen Bromide DAYS LIVING RADIOISOTOPES DOMESTIC ANIMALS ENZYME ACTIVITY ENZYMES Eye Proteins - isolation & purification Eye Proteins - metabolism EYES FACE GTP-Binding Protein Regulators HEAD IMMUNOASSAY ISOTOPE APPLICATIONS ISOTOPES KINETICS LIGHT NUCLEI LILIOPSIDA LIQUID COLUMN CHROMATOGRAPHY MAGNOLIOPHYTA MAMMALS Molecular Sequence Data MOLECULAR STRUCTURE MONOCLONAL ANTIBODIES NUCLEI NUCLEOTIDES ODD-ODD NUCLEI ORGANIC COMPOUNDS ORGANS Peptide Fragments - isolation & purification PEPTIDES phosducin Phosphopeptides - isolation & purification Phosphoproteins - isolation & purification Phosphoproteins - metabolism PHOSPHORUS 32 PHOSPHORUS ISOTOPES Phosphorus Radioisotopes PHOSPHORUS-GROUP TRANSFERASES PHOSPHORYLATION PHOSPHOTRANSFERASES PLANTS Protein Kinases - metabolism PROTEINS RADIOISOTOPES RATS Rats, Inbred Strains REACTION KINETICS RETINA Retina - metabolism RODENTS RUMINANTS SENSE ORGANS SEPARATION PROCESSES Substrate Specificity TRACER TECHNIQUES TRANSFERASES VERTEBRATES
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