Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry
An analytical method that was developed to analyze deuterium‐labeled fatty acids in human blood has been extended to identify labeled fatty acids from C14 to C24 chain length which are formed by metabolic processes such as desaturation, elongation, or shortening of the labeled fatty acids fed. A new...
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| Veröffentlicht in: | Lipids 1990-07, Vol.25 (7), p.401-405 |
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| creator | Rohwedder, W.K Duval, S.M Wolf, D.J Emken, E.A |
| description | An analytical method that was developed to analyze deuterium‐labeled fatty acids in human blood has been extended to identify labeled fatty acids from C14 to C24 chain length which are formed by metabolic processes such as desaturation, elongation, or shortening of the labeled fatty acids fed. A new computer and a hard‐wave adder have been utilized to assure reliable data acquisition. Relative standard derivations for the analysis of labeled fatty acids were measured at 0.02, 0.03, and 0.04 at the 5%, 1%, and 0.2% levels of the labeled fatty acid methyl esters, respectively. The method makes extensive use of standards and computer processing for accuracy and high productivity. Data from a chylomicron triacylglycerol fraction are included to demonstrate the sensitivity of detection of metabolites formed by desaturation and elongation. |
| doi_str_mv | 10.1007/BF02537984 |
| format | Article |
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A new computer and a hard‐wave adder have been utilized to assure reliable data acquisition. Relative standard derivations for the analysis of labeled fatty acids were measured at 0.02, 0.03, and 0.04 at the 5%, 1%, and 0.2% levels of the labeled fatty acid methyl esters, respectively. The method makes extensive use of standards and computer processing for accuracy and high productivity. 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Psychology ; GAS CHROMATOGRAPHY ; Gas Chromatography-Mass Spectrometry ; Humans ; HYDROGEN ISOTOPES ; ISOTOPE APPLICATIONS ; ISOTOPES ; labeling ; LIGHT NUCLEI ; Lipids ; MAMMALS ; MAN ; mass spectrometry ; MASS SPECTROSCOPY ; MATERIALS ; METABOLISM ; METABOLITES ; NUCLEI ; ODD-ODD NUCLEI ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; Other biological molecules ; PRIMATES ; PROCESSING ; SEPARATION PROCESSES ; SPECTROSCOPY ; STABLE ISOTOPES ; TRACER TECHNIQUES ; triacylglycerols ; VERTEBRATES</subject><ispartof>Lipids, 1990-07, Vol.25 (7), p.401-405</ispartof><rights>1990 American Oil Chemists' Society (AOCS)</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4041-a6227f2f358ad3bbbfbc4a6d755b0bf413ce9f2c475508d62635a419e67e7ee53</citedby><cites>FETCH-LOGICAL-c4041-a6227f2f358ad3bbbfbc4a6d755b0bf413ce9f2c475508d62635a419e67e7ee53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19293754$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2395419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6369276$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Rohwedder, W.K</creatorcontrib><creatorcontrib>Duval, S.M</creatorcontrib><creatorcontrib>Wolf, D.J</creatorcontrib><creatorcontrib>Emken, E.A</creatorcontrib><title>Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry</title><title>Lipids</title><addtitle>Lipids</addtitle><description>An analytical method that was developed to analyze deuterium‐labeled fatty acids in human blood has been extended to identify labeled fatty acids from C14 to C24 chain length which are formed by metabolic processes such as desaturation, elongation, or shortening of the labeled fatty acids fed. 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Data from a chylomicron triacylglycerol fraction are included to demonstrate the sensitivity of detection of metabolites formed by desaturation and elongation.</description><subject>550501 - Metabolism- Tracer Techniques</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BLOOD</subject><subject>BODY FLUIDS</subject><subject>CARBOXYLIC ACIDS</subject><subject>CHROMATOGRAPHY</subject><subject>DATA PROCESSING</subject><subject>DEUTERIUM</subject><subject>Electronic Data Processing</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAS CHROMATOGRAPHY</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>HYDROGEN ISOTOPES</subject><subject>ISOTOPE APPLICATIONS</subject><subject>ISOTOPES</subject><subject>labeling</subject><subject>LIGHT NUCLEI</subject><subject>Lipids</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>mass spectrometry</subject><subject>MASS SPECTROSCOPY</subject><subject>MATERIALS</subject><subject>METABOLISM</subject><subject>METABOLITES</subject><subject>NUCLEI</subject><subject>ODD-ODD NUCLEI</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>Other biological molecules</subject><subject>PRIMATES</subject><subject>PROCESSING</subject><subject>SEPARATION PROCESSES</subject><subject>SPECTROSCOPY</subject><subject>STABLE ISOTOPES</subject><subject>TRACER TECHNIQUES</subject><subject>triacylglycerols</subject><subject>VERTEBRATES</subject><issn>0024-4201</issn><issn>1558-9307</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kk1v1DAQhi0EKtvChTsiQioHpFB_xusjFAqVFoEEPUcTZ7xrlMRb2wHl3-PVruiNk-V3nnnHM2NCXjD6jlGqrz7cUK6ENmv5iKyYUuvaCKofkxWlXNaSU_aUnKf0q1yZNOqMnHFhlGRmReavCGmOOOKUq-CqvMNqxAxdGLyt_JQx2jD9xph8mA5Aj3PR_DzWA3Q4YF85yHmpwPo-Vd1SbSFVdhfDCDlsI-x3y9UIKVVpjzYXGXNcnpEnDoaEz0_nBbm7-fTz-ku9-fb59vr9praSSlZDw7l23Am1hl50Xec6K6HptVId7ZxkwqJx3Moi0HXf8EYoKG1ho1EjKnFBXh99Q8q-TdZntLvSz1Se0jaiMVw3BXpzhPYx3M-Ycjv6ZHEYYMIwp1YbU0araAHfHkEbQ0oRXbuPfoS4tIy2h0W0D4so8MuT69yN2P9DT5Mv8ctTHJKFwUWYrE8PjoYbodXBhx65P37A5T8V283t949UUlZSXh1THIQWtrHY3v0on0BQrimjohF_AQ7JqRw</recordid><startdate>199007</startdate><enddate>199007</enddate><creator>Rohwedder, W.K</creator><creator>Duval, S.M</creator><creator>Wolf, D.J</creator><creator>Emken, E.A</creator><general>Springer‐Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>199007</creationdate><title>Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry</title><author>Rohwedder, W.K ; Duval, S.M ; Wolf, D.J ; Emken, E.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4041-a6227f2f358ad3bbbfbc4a6d755b0bf413ce9f2c475508d62635a419e67e7ee53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>550501 - Metabolism- Tracer Techniques</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BLOOD</topic><topic>BODY FLUIDS</topic><topic>CARBOXYLIC ACIDS</topic><topic>CHROMATOGRAPHY</topic><topic>DATA PROCESSING</topic><topic>DEUTERIUM</topic><topic>Electronic Data Processing</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAS CHROMATOGRAPHY</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>HYDROGEN ISOTOPES</topic><topic>ISOTOPE APPLICATIONS</topic><topic>ISOTOPES</topic><topic>labeling</topic><topic>LIGHT NUCLEI</topic><topic>Lipids</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>mass spectrometry</topic><topic>MASS SPECTROSCOPY</topic><topic>MATERIALS</topic><topic>METABOLISM</topic><topic>METABOLITES</topic><topic>NUCLEI</topic><topic>ODD-ODD NUCLEI</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>Other biological molecules</topic><topic>PRIMATES</topic><topic>PROCESSING</topic><topic>SEPARATION PROCESSES</topic><topic>SPECTROSCOPY</topic><topic>STABLE ISOTOPES</topic><topic>TRACER TECHNIQUES</topic><topic>triacylglycerols</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rohwedder, W.K</creatorcontrib><creatorcontrib>Duval, S.M</creatorcontrib><creatorcontrib>Wolf, D.J</creatorcontrib><creatorcontrib>Emken, E.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Lipids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rohwedder, W.K</au><au>Duval, S.M</au><au>Wolf, D.J</au><au>Emken, E.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry</atitle><jtitle>Lipids</jtitle><addtitle>Lipids</addtitle><date>1990-07</date><risdate>1990</risdate><volume>25</volume><issue>7</issue><spage>401</spage><epage>405</epage><pages>401-405</pages><issn>0024-4201</issn><eissn>1558-9307</eissn><coden>LPDSAP</coden><abstract>An analytical method that was developed to analyze deuterium‐labeled fatty acids in human blood has been extended to identify labeled fatty acids from C14 to C24 chain length which are formed by metabolic processes such as desaturation, elongation, or shortening of the labeled fatty acids fed. A new computer and a hard‐wave adder have been utilized to assure reliable data acquisition. Relative standard derivations for the analysis of labeled fatty acids were measured at 0.02, 0.03, and 0.04 at the 5%, 1%, and 0.2% levels of the labeled fatty acid methyl esters, respectively. The method makes extensive use of standards and computer processing for accuracy and high productivity. Data from a chylomicron triacylglycerol fraction are included to demonstrate the sensitivity of detection of metabolites formed by desaturation and elongation.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer‐Verlag</pub><pmid>2395419</pmid><doi>10.1007/BF02537984</doi><tpages>5</tpages></addata></record> |
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| ispartof | Lipids, 1990-07, Vol.25 (7), p.401-405 |
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| subjects | 550501 - Metabolism- Tracer Techniques Analytical, structural and metabolic biochemistry ANIMALS BASIC BIOLOGICAL SCIENCES Biological and medical sciences BIOLOGICAL MATERIALS BLOOD BODY FLUIDS CARBOXYLIC ACIDS CHROMATOGRAPHY DATA PROCESSING DEUTERIUM Electronic Data Processing Fatty acids Fatty Acids - metabolism Fundamental and applied biological sciences. Psychology GAS CHROMATOGRAPHY Gas Chromatography-Mass Spectrometry Humans HYDROGEN ISOTOPES ISOTOPE APPLICATIONS ISOTOPES labeling LIGHT NUCLEI Lipids MAMMALS MAN mass spectrometry MASS SPECTROSCOPY MATERIALS METABOLISM METABOLITES NUCLEI ODD-ODD NUCLEI ORGANIC ACIDS ORGANIC COMPOUNDS Other biological molecules PRIMATES PROCESSING SEPARATION PROCESSES SPECTROSCOPY STABLE ISOTOPES TRACER TECHNIQUES triacylglycerols VERTEBRATES |
| title | Measurement of the metabolic interconversion of deuterium-labeled fatty acids by gas chromatography/mass spectrometry |
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