Purification and characterization of lutropin receptor from membranes of pig follicular fluid

Membranes derived from free floating granulosa cells in porcine ovarian follicular fluid were used as a starting material for structural characterization of both LH/hCG and FSH receptors. The receptors were highly hormone-specific and showed single classes of high-affinity binding sites (Kd = 19-74...

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Veröffentlicht in:	Biochemistry (Easton) 1990-04, Vol.29 (15), p.3751-3759
Hauptverfasser:	Yarney, T. A, Sairam, M. R, Bhargavi, G. N, Mohapatra, S. K
Format:	Artikel
Sprache:	eng
Schlagworte:	550601 - Medicine- Unsealed Radionuclides in Diagnostics ANIMAL CELLS ANIMALS AUTORADIOGRAPHY BETA DECAY RADIOISOTOPES binding Binding, Competitive Biological and medical sciences BODY CELL CONSTITUENTS Cell Membrane - analysis CELL MEMBRANES Cell receptors Cell structures and functions CHEMICAL REACTIONS Chromatography, Affinity CROSS-LINKING Cross-Linking Reagents - metabolism DAYS LIVING RADIOISOTOPES DOMESTIC ANIMALS ELECTRON CAPTURE RADIOISOTOPES Female FEMALE GENITALS follicle-stimulating hormone follicular fluid Follicular Fluid - analysis FSH Fundamental and applied biological sciences. Psychology GONADOTROPINS GONADS Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors hormone specificity HORMONES INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES isolation ISOTOPES LH LIGANDS luteinizing hormone MAMMALS MEMBRANE PROTEINS MEMBRANES Molecular and cellular biology MOLECULAR STRUCTURE MOLECULAR WEIGHT NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANS OVARIES PEPTIDE HORMONES PEPTIDES PITUITARY HORMONES plasma membranes POLYMERIZATION POLYPEPTIDES PROTEINS PURIFICATION RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE RECEPTORS Receptors, FSH - metabolism Receptors, Gonadotropin - metabolism Receptors, LH - analysis Solubility SWINE TUMOR CELLS VERTEBRATES
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