Phosphorylation of chicken growth hormone
The possibility that chicken growth hormone (cGH) can be phosphorylated has been examined. Both native and biosynthetic cGH were phosphorylated by cAMP-dependent protein kinase (and π- 32P-ATP). The extent of phosphorylation was however less than that observed with ovine prolactin. Under the conditi...
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| Veröffentlicht in: | Life sciences (1973) 1990, Vol.47 (11), p.945-952 |
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| creator | Aramburo, C. Donoghue, D. Montiel, J.L. Berghman, L.R. Scanes, C.G. |
| description | The possibility that chicken growth hormone (cGH) can be phosphorylated has been examined. Both native and biosynthetic cGH were phosphorylated by cAMP-dependent protein kinase (and π-
32P-ATP). The extent of phosphorylation was however less than that observed with ovine prolactin. Under the conditions employed, glycosylated cGH was not phosphorylated. Chicken anterior pituitary cells in primary culture were incubated in the presence of
32P-phosphate. Radioactive phosphate was incorporated
in vitro
into the fraction immunoprecipitable with antisera against cGH. Incorporation was increased with cell number and time of incubation. The presence of GH releasing factor (GRF) increased the release of
32P-phosphate labelled immunoprecipitable GH into the incubation media but not content of immunoprecipitable GH in the cells. The molecular weight of the phosphorylated immunoreactive cGH in the cells corresponded to cGH dimer. |
| doi_str_mv | 10.1016/0024-3205(90)90541-X |
| format | Article |
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32P-ATP). The extent of phosphorylation was however less than that observed with ovine prolactin. Under the conditions employed, glycosylated cGH was not phosphorylated. Chicken anterior pituitary cells in primary culture were incubated in the presence of
32P-phosphate. Radioactive phosphate was incorporated
in vitro
into the fraction immunoprecipitable with antisera against cGH. Incorporation was increased with cell number and time of incubation. The presence of GH releasing factor (GRF) increased the release of
32P-phosphate labelled immunoprecipitable GH into the incubation media but not content of immunoprecipitable GH in the cells. The molecular weight of the phosphorylated immunoreactive cGH in the cells corresponded to cGH dimer.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(90)90541-X</identifier><identifier>PMID: 2215076</identifier><identifier>CODEN: LIFSAK</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>550501 - Metabolism- Tracer Techniques ; AMP ; Analytical, structural and metabolic biochemistry ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; BIOASSAY ; Biological and medical sciences ; BIRDS ; Cells, Cultured ; CHEMICAL REACTIONS ; CHICKENS ; DAYS LIVING RADIOISOTOPES ; Electrophoresis, Polyacrylamide Gel ; ENZYMES ; FOWL ; Fundamental and applied biological sciences. Psychology ; growth hormone ; Growth Hormone - metabolism ; HORMONES ; IMMUNOASSAY ; ISOTOPE APPLICATIONS ; ISOTOPES ; LIGHT NUCLEI ; Male ; MOLECULAR WEIGHT ; NUCLEI ; NUCLEOTIDES ; ODD-ODD NUCLEI ; ORGANIC COMPOUNDS ; OXYGEN COMPOUNDS ; PEPTIDE HORMONES ; PHOSPHATES ; PHOSPHORUS 32 ; PHOSPHORUS COMPOUNDS ; PHOSPHORUS ISOTOPES ; PHOSPHORUS-GROUP TRANSFERASES ; PHOSPHORYLATION ; PHOSPHOTRANSFERASES ; Pituitary Gland, Anterior - cytology ; Pituitary Gland, Anterior - metabolism ; PITUITARY HORMONES ; Precipitin Tests ; Protein hormones. Growth factors. Cytokines ; Protein Kinases - metabolism ; Proteins ; RADIOISOTOPES ; STH ; TRACER TECHNIQUES ; TRANSFERASES ; VERTEBRATES</subject><ispartof>Life sciences (1973), 1990, Vol.47 (11), p.945-952</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-b2eb810f851352fe98889ffc4ef0db534209bbefc97d136c1753cd7315226d443</citedby><cites>FETCH-LOGICAL-c445t-b2eb810f851352fe98889ffc4ef0db534209bbefc97d136c1753cd7315226d443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0024-3205(90)90541-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19391849$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2215076$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6253710$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Aramburo, C.</creatorcontrib><creatorcontrib>Donoghue, D.</creatorcontrib><creatorcontrib>Montiel, J.L.</creatorcontrib><creatorcontrib>Berghman, L.R.</creatorcontrib><creatorcontrib>Scanes, C.G.</creatorcontrib><title>Phosphorylation of chicken growth hormone</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>The possibility that chicken growth hormone (cGH) can be phosphorylated has been examined. Both native and biosynthetic cGH were phosphorylated by cAMP-dependent protein kinase (and π-
32P-ATP). The extent of phosphorylation was however less than that observed with ovine prolactin. Under the conditions employed, glycosylated cGH was not phosphorylated. Chicken anterior pituitary cells in primary culture were incubated in the presence of
32P-phosphate. Radioactive phosphate was incorporated
in vitro
into the fraction immunoprecipitable with antisera against cGH. Incorporation was increased with cell number and time of incubation. The presence of GH releasing factor (GRF) increased the release of
32P-phosphate labelled immunoprecipitable GH into the incubation media but not content of immunoprecipitable GH in the cells. The molecular weight of the phosphorylated immunoreactive cGH in the cells corresponded to cGH dimer.</description><subject>550501 - Metabolism- Tracer Techniques</subject><subject>AMP</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>BIOASSAY</subject><subject>Biological and medical sciences</subject><subject>BIRDS</subject><subject>Cells, Cultured</subject><subject>CHEMICAL REACTIONS</subject><subject>CHICKENS</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>ENZYMES</subject><subject>FOWL</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>growth hormone</subject><subject>Growth Hormone - metabolism</subject><subject>HORMONES</subject><subject>IMMUNOASSAY</subject><subject>ISOTOPE APPLICATIONS</subject><subject>ISOTOPES</subject><subject>LIGHT NUCLEI</subject><subject>Male</subject><subject>MOLECULAR WEIGHT</subject><subject>NUCLEI</subject><subject>NUCLEOTIDES</subject><subject>ODD-ODD NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>OXYGEN COMPOUNDS</subject><subject>PEPTIDE HORMONES</subject><subject>PHOSPHATES</subject><subject>PHOSPHORUS 32</subject><subject>PHOSPHORUS COMPOUNDS</subject><subject>PHOSPHORUS ISOTOPES</subject><subject>PHOSPHORUS-GROUP TRANSFERASES</subject><subject>PHOSPHORYLATION</subject><subject>PHOSPHOTRANSFERASES</subject><subject>Pituitary Gland, Anterior - cytology</subject><subject>Pituitary Gland, Anterior - metabolism</subject><subject>PITUITARY HORMONES</subject><subject>Precipitin Tests</subject><subject>Protein hormones. Growth factors. Cytokines</subject><subject>Protein Kinases - metabolism</subject><subject>Proteins</subject><subject>RADIOISOTOPES</subject><subject>STH</subject><subject>TRACER TECHNIQUES</subject><subject>TRANSFERASES</subject><subject>VERTEBRATES</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq0KRBfoPyjSCqmoHELHX7F9qYRWbUFCKgcqcbMSZ0xcsvFiZ0H8-ybdFdzgNIf3mVczDyGfKZxRoOU3ACYKzkB-NXBqQApa3H4gM6qVKaDkdIfMXpCPZD_nvwAgpeJ7ZI8xKkGVM3J63ca8amN67qohxH4e_dy1wd1jP79L8Wlo52O4jD0ekl1fdRk_becB-fPzx83iorj6_etycX5VOCHkUNQMa03Ba0m5ZB6N1tp47wR6aGrJBQNT1-idUQ3lpaNKctcoTiVjZSMEPyDHm96Yh2CzCwO61sW-RzfYkkmuKIzQyQZapfiwxjzYZcgOu67qMa6z1QCCKyXeBak0oE05NYoN6FLMOaG3qxSWVXq2FOzk204y7STTGrD_fdvbce1o27-ul9i8LG0Fj_mXbV5lV3U-Vb0L-bXbcEO1MCP3fcPhqPYxYJo-x95hE9L0eBPD24f8A4jnmbw</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Aramburo, C.</creator><creator>Donoghue, D.</creator><creator>Montiel, J.L.</creator><creator>Berghman, L.R.</creator><creator>Scanes, C.G.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1990</creationdate><title>Phosphorylation of chicken growth hormone</title><author>Aramburo, C. ; Donoghue, D. ; Montiel, J.L. ; Berghman, L.R. ; Scanes, C.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-b2eb810f851352fe98889ffc4ef0db534209bbefc97d136c1753cd7315226d443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>550501 - Metabolism- Tracer Techniques</topic><topic>AMP</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>BIOASSAY</topic><topic>Biological and medical sciences</topic><topic>BIRDS</topic><topic>Cells, Cultured</topic><topic>CHEMICAL REACTIONS</topic><topic>CHICKENS</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>ENZYMES</topic><topic>FOWL</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>growth hormone</topic><topic>Growth Hormone - metabolism</topic><topic>HORMONES</topic><topic>IMMUNOASSAY</topic><topic>ISOTOPE APPLICATIONS</topic><topic>ISOTOPES</topic><topic>LIGHT NUCLEI</topic><topic>Male</topic><topic>MOLECULAR WEIGHT</topic><topic>NUCLEI</topic><topic>NUCLEOTIDES</topic><topic>ODD-ODD NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>OXYGEN COMPOUNDS</topic><topic>PEPTIDE HORMONES</topic><topic>PHOSPHATES</topic><topic>PHOSPHORUS 32</topic><topic>PHOSPHORUS COMPOUNDS</topic><topic>PHOSPHORUS ISOTOPES</topic><topic>PHOSPHORUS-GROUP TRANSFERASES</topic><topic>PHOSPHORYLATION</topic><topic>PHOSPHOTRANSFERASES</topic><topic>Pituitary Gland, Anterior - cytology</topic><topic>Pituitary Gland, Anterior - metabolism</topic><topic>PITUITARY HORMONES</topic><topic>Precipitin Tests</topic><topic>Protein hormones. Growth factors. Cytokines</topic><topic>Protein Kinases - metabolism</topic><topic>Proteins</topic><topic>RADIOISOTOPES</topic><topic>STH</topic><topic>TRACER TECHNIQUES</topic><topic>TRANSFERASES</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aramburo, C.</creatorcontrib><creatorcontrib>Donoghue, D.</creatorcontrib><creatorcontrib>Montiel, J.L.</creatorcontrib><creatorcontrib>Berghman, L.R.</creatorcontrib><creatorcontrib>Scanes, C.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aramburo, C.</au><au>Donoghue, D.</au><au>Montiel, J.L.</au><au>Berghman, L.R.</au><au>Scanes, C.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphorylation of chicken growth hormone</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1990</date><risdate>1990</risdate><volume>47</volume><issue>11</issue><spage>945</spage><epage>952</epage><pages>945-952</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><coden>LIFSAK</coden><abstract>The possibility that chicken growth hormone (cGH) can be phosphorylated has been examined. Both native and biosynthetic cGH were phosphorylated by cAMP-dependent protein kinase (and π-
32P-ATP). The extent of phosphorylation was however less than that observed with ovine prolactin. Under the conditions employed, glycosylated cGH was not phosphorylated. Chicken anterior pituitary cells in primary culture were incubated in the presence of
32P-phosphate. Radioactive phosphate was incorporated
in vitro
into the fraction immunoprecipitable with antisera against cGH. Incorporation was increased with cell number and time of incubation. The presence of GH releasing factor (GRF) increased the release of
32P-phosphate labelled immunoprecipitable GH into the incubation media but not content of immunoprecipitable GH in the cells. The molecular weight of the phosphorylated immunoreactive cGH in the cells corresponded to cGH dimer.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>2215076</pmid><doi>10.1016/0024-3205(90)90541-X</doi><tpages>8</tpages></addata></record> |
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| ispartof | Life sciences (1973), 1990, Vol.47 (11), p.945-952 |
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| recordid | cdi_osti_scitechconnect_6253710 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 550501 - Metabolism- Tracer Techniques AMP Analytical, structural and metabolic biochemistry ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOASSAY Biological and medical sciences BIRDS Cells, Cultured CHEMICAL REACTIONS CHICKENS DAYS LIVING RADIOISOTOPES Electrophoresis, Polyacrylamide Gel ENZYMES FOWL Fundamental and applied biological sciences. Psychology growth hormone Growth Hormone - metabolism HORMONES IMMUNOASSAY ISOTOPE APPLICATIONS ISOTOPES LIGHT NUCLEI Male MOLECULAR WEIGHT NUCLEI NUCLEOTIDES ODD-ODD NUCLEI ORGANIC COMPOUNDS OXYGEN COMPOUNDS PEPTIDE HORMONES PHOSPHATES PHOSPHORUS 32 PHOSPHORUS COMPOUNDS PHOSPHORUS ISOTOPES PHOSPHORUS-GROUP TRANSFERASES PHOSPHORYLATION PHOSPHOTRANSFERASES Pituitary Gland, Anterior - cytology Pituitary Gland, Anterior - metabolism PITUITARY HORMONES Precipitin Tests Protein hormones. Growth factors. Cytokines Protein Kinases - metabolism Proteins RADIOISOTOPES STH TRACER TECHNIQUES TRANSFERASES VERTEBRATES |
| title | Phosphorylation of chicken growth hormone |
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