Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions
We have examined the antigenicity of plasma membrane (PM) and rough microsomal (RM) fractions from rat liver using anti- ras monoclonal antibodies 142-24E05 and Y13-259 and immunochemistry as well as electron microscope immunocytochemistry. Proteins immunoprecipitated with monoclonal antibody 142-24...
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| Veröffentlicht in: | Experimental cell research 1991, Vol.192 (1), p.137-147 |
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| creator | Dominguez, J.Manuel Lanoix, J. Paiement, Jacques |
| description | We have examined the antigenicity of plasma membrane (PM) and rough microsomal (RM) fractions from rat liver using anti-
ras monoclonal antibodies 142-24E05 and Y13-259 and immunochemistry as well as electron microscope immunocytochemistry. Proteins immunoprecipitated with monoclonal antibody 142-24E05 were separated using single-dimensional gradient-gel electrophoresis. The separated proteins were then blotted onto nitrocellulose sheets and incubated with [α-
32P]GTP. Radioautograms of blots indicated the presence of specific 21.5- and 22-kDa labeled proteins in the PM fraction. A 23.5-kDa [α-
32P] GTP-binding protein was detected in immunoprecipitates of both PM and RM fractions. Monoclonal antibody Y13-259 reacted only with the 21.5-kDa [α-
32P] GTP-binding protein in the plasma membrane fraction. When anti-
ras monoclonal antibody 142-24E05 and the immunogold technique were applied to membrane fractions using a preembedding immunocytochemical method, specific labeling was observed in association with both vesicular structures and membrane sheets in the PM fraction but only with electron-dense vesicular structures in the RM fraction. Thus
ras antigenicity is associated with hepatocyte plasma membranes and
ras-like antigenicity is probably associated with vesicular (secretory/endocytic) elements in both plasma membrane and rough microsomal preparations. |
| doi_str_mv | 10.1016/0014-4827(91)90168-T |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_6152563</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>001448279190168T</els_id><sourcerecordid>80366082</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-658bd59f2f648781455afc938ef5f1d06567f1d041cfc6384a9d0d9d864c74e03</originalsourceid><addsrcrecordid>eNqFkU-LFDEQxYMo67j6DRSCoOihNelOMsllQRb_wYCX8RwySWUn0p2MSVoYP73pmcG96amg6lcvefUQek7JO0qoeE8IZR2T_fqNom9V68hu-wCtKFGk61nfP0Srv8hj9KSUH4QQKam4QldUKsmlWqFxk6wZw29TQ4o4eZxNwSbWcAcx2FCPOMTWq3gPB1OTPVbAh9GUyeAJpl02ERrucE7z3R5DdOk0DRZnqMHO4zxhn41d5MtT9MibscCzS71G3z993N5-6TbfPn-9_bDpLGO8doLLnePK914wuZaUcW68VYMEzz11RHCxXiqj1lsxSGaUI045KZhdMyDDNXp51k2lBl2aDbB7m2IEW7WgvOdiaNDrM3TI6ecMpeopFAvj2CyluWhJBiGI7P8LUq7IwBhtIDuDNqdSMnh9yGEy-agp0UtkeslDL3loRfUpMr1tay8u-vNuAne_dM6ozV9d5qa0rNo1ow3lHlOCkf70_M2Zg3baXwHy4hyiBRfyYtyl8O-P_AEgcbNj</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15903441</pqid></control><display><type>article</type><title>Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Dominguez, J.Manuel ; Lanoix, J. ; Paiement, Jacques</creator><creatorcontrib>Dominguez, J.Manuel ; Lanoix, J. ; Paiement, Jacques</creatorcontrib><description>We have examined the antigenicity of plasma membrane (PM) and rough microsomal (RM) fractions from rat liver using anti-
ras monoclonal antibodies 142-24E05 and Y13-259 and immunochemistry as well as electron microscope immunocytochemistry. Proteins immunoprecipitated with monoclonal antibody 142-24E05 were separated using single-dimensional gradient-gel electrophoresis. The separated proteins were then blotted onto nitrocellulose sheets and incubated with [α-
32P]GTP. Radioautograms of blots indicated the presence of specific 21.5- and 22-kDa labeled proteins in the PM fraction. A 23.5-kDa [α-
32P] GTP-binding protein was detected in immunoprecipitates of both PM and RM fractions. Monoclonal antibody Y13-259 reacted only with the 21.5-kDa [α-
32P] GTP-binding protein in the plasma membrane fraction. When anti-
ras monoclonal antibody 142-24E05 and the immunogold technique were applied to membrane fractions using a preembedding immunocytochemical method, specific labeling was observed in association with both vesicular structures and membrane sheets in the PM fraction but only with electron-dense vesicular structures in the RM fraction. Thus
ras antigenicity is associated with hepatocyte plasma membranes and
ras-like antigenicity is probably associated with vesicular (secretory/endocytic) elements in both plasma membrane and rough microsomal preparations.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(91)90168-T</identifier><identifier>PMID: 1898589</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>550201 - Biochemistry- Tracer Techniques ; ANIMAL CELLS ; Animals ; ANTIBODIES ; Antibodies, Monoclonal ; ANTIGEN-ANTIBODY REACTIONS ; AUTORADIOGRAPHY ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Biological and medical sciences ; BIOLOGICAL LOCALIZATION ; CELL CONSTITUENTS ; Cell Membrane - chemistry ; CELL MEMBRANES ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; Cross Reactions ; DAYS LIVING RADIOISOTOPES ; ELECTROPHORESIS ; ENDOPLASMIC RETICULUM ; Endoplasmic Reticulum - chemistry ; Fundamental and applied biological sciences. Psychology ; GTP-Binding Proteins - immunology ; hepatocytes ; Immunohistochemistry ; ISOTOPES ; LIGHT NUCLEI ; Liver - chemistry ; LIVER CELLS ; MEMBRANES ; MICROSOMES ; Molecular and cellular biology ; Molecular Weight ; MONOCLONAL ANTIBODIES ; NUCLEI ; ODD-ODD NUCLEI ; Oncogene Protein p21(ras) - analysis ; ORGANIC COMPOUNDS ; ORGANOIDS ; PHOSPHORUS 32 ; PHOSPHORUS ISOTOPES ; Phosphorus Radioisotopes ; plasma membranes ; Precipitin Tests ; protein p21 ; PROTEINS ; RADIOISOTOPES ; ras ; Rats ; RIBOSOMES ; SOMATIC CELLS ; Subcellular Fractions - chemistry</subject><ispartof>Experimental cell research, 1991, Vol.192 (1), p.137-147</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-658bd59f2f648781455afc938ef5f1d06567f1d041cfc6384a9d0d9d864c74e03</citedby><cites>FETCH-LOGICAL-c445t-658bd59f2f648781455afc938ef5f1d06567f1d041cfc6384a9d0d9d864c74e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(91)90168-T$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19640241$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1898589$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6152563$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Dominguez, J.Manuel</creatorcontrib><creatorcontrib>Lanoix, J.</creatorcontrib><creatorcontrib>Paiement, Jacques</creatorcontrib><title>Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>We have examined the antigenicity of plasma membrane (PM) and rough microsomal (RM) fractions from rat liver using anti-
ras monoclonal antibodies 142-24E05 and Y13-259 and immunochemistry as well as electron microscope immunocytochemistry. Proteins immunoprecipitated with monoclonal antibody 142-24E05 were separated using single-dimensional gradient-gel electrophoresis. The separated proteins were then blotted onto nitrocellulose sheets and incubated with [α-
32P]GTP. Radioautograms of blots indicated the presence of specific 21.5- and 22-kDa labeled proteins in the PM fraction. A 23.5-kDa [α-
32P] GTP-binding protein was detected in immunoprecipitates of both PM and RM fractions. Monoclonal antibody Y13-259 reacted only with the 21.5-kDa [α-
32P] GTP-binding protein in the plasma membrane fraction. When anti-
ras monoclonal antibody 142-24E05 and the immunogold technique were applied to membrane fractions using a preembedding immunocytochemical method, specific labeling was observed in association with both vesicular structures and membrane sheets in the PM fraction but only with electron-dense vesicular structures in the RM fraction. Thus
ras antigenicity is associated with hepatocyte plasma membranes and
ras-like antigenicity is probably associated with vesicular (secretory/endocytic) elements in both plasma membrane and rough microsomal preparations.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>ANIMAL CELLS</subject><subject>Animals</subject><subject>ANTIBODIES</subject><subject>Antibodies, Monoclonal</subject><subject>ANTIGEN-ANTIBODY REACTIONS</subject><subject>AUTORADIOGRAPHY</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL LOCALIZATION</subject><subject>CELL CONSTITUENTS</subject><subject>Cell Membrane - chemistry</subject><subject>CELL MEMBRANES</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>Cross Reactions</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>ELECTROPHORESIS</subject><subject>ENDOPLASMIC RETICULUM</subject><subject>Endoplasmic Reticulum - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GTP-Binding Proteins - immunology</subject><subject>hepatocytes</subject><subject>Immunohistochemistry</subject><subject>ISOTOPES</subject><subject>LIGHT NUCLEI</subject><subject>Liver - chemistry</subject><subject>LIVER CELLS</subject><subject>MEMBRANES</subject><subject>MICROSOMES</subject><subject>Molecular and cellular biology</subject><subject>Molecular Weight</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>NUCLEI</subject><subject>ODD-ODD NUCLEI</subject><subject>Oncogene Protein p21(ras) - analysis</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANOIDS</subject><subject>PHOSPHORUS 32</subject><subject>PHOSPHORUS ISOTOPES</subject><subject>Phosphorus Radioisotopes</subject><subject>plasma membranes</subject><subject>Precipitin Tests</subject><subject>protein p21</subject><subject>PROTEINS</subject><subject>RADIOISOTOPES</subject><subject>ras</subject><subject>Rats</subject><subject>RIBOSOMES</subject><subject>SOMATIC CELLS</subject><subject>Subcellular Fractions - chemistry</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-LFDEQxYMo67j6DRSCoOihNelOMsllQRb_wYCX8RwySWUn0p2MSVoYP73pmcG96amg6lcvefUQek7JO0qoeE8IZR2T_fqNom9V68hu-wCtKFGk61nfP0Srv8hj9KSUH4QQKam4QldUKsmlWqFxk6wZw29TQ4o4eZxNwSbWcAcx2FCPOMTWq3gPB1OTPVbAh9GUyeAJpl02ERrucE7z3R5DdOk0DRZnqMHO4zxhn41d5MtT9MibscCzS71G3z993N5-6TbfPn-9_bDpLGO8doLLnePK914wuZaUcW68VYMEzz11RHCxXiqj1lsxSGaUI045KZhdMyDDNXp51k2lBl2aDbB7m2IEW7WgvOdiaNDrM3TI6ecMpeopFAvj2CyluWhJBiGI7P8LUq7IwBhtIDuDNqdSMnh9yGEy-agp0UtkeslDL3loRfUpMr1tay8u-vNuAne_dM6ozV9d5qa0rNo1ow3lHlOCkf70_M2Zg3baXwHy4hyiBRfyYtyl8O-P_AEgcbNj</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Dominguez, J.Manuel</creator><creator>Lanoix, J.</creator><creator>Paiement, Jacques</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1991</creationdate><title>Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions</title><author>Dominguez, J.Manuel ; Lanoix, J. ; Paiement, Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-658bd59f2f648781455afc938ef5f1d06567f1d041cfc6384a9d0d9d864c74e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>ANIMAL CELLS</topic><topic>Animals</topic><topic>ANTIBODIES</topic><topic>Antibodies, Monoclonal</topic><topic>ANTIGEN-ANTIBODY REACTIONS</topic><topic>AUTORADIOGRAPHY</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL LOCALIZATION</topic><topic>CELL CONSTITUENTS</topic><topic>Cell Membrane - chemistry</topic><topic>CELL MEMBRANES</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>Cross Reactions</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>ELECTROPHORESIS</topic><topic>ENDOPLASMIC RETICULUM</topic><topic>Endoplasmic Reticulum - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GTP-Binding Proteins - immunology</topic><topic>hepatocytes</topic><topic>Immunohistochemistry</topic><topic>ISOTOPES</topic><topic>LIGHT NUCLEI</topic><topic>Liver - chemistry</topic><topic>LIVER CELLS</topic><topic>MEMBRANES</topic><topic>MICROSOMES</topic><topic>Molecular and cellular biology</topic><topic>Molecular Weight</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>NUCLEI</topic><topic>ODD-ODD NUCLEI</topic><topic>Oncogene Protein p21(ras) - analysis</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANOIDS</topic><topic>PHOSPHORUS 32</topic><topic>PHOSPHORUS ISOTOPES</topic><topic>Phosphorus Radioisotopes</topic><topic>plasma membranes</topic><topic>Precipitin Tests</topic><topic>protein p21</topic><topic>PROTEINS</topic><topic>RADIOISOTOPES</topic><topic>ras</topic><topic>Rats</topic><topic>RIBOSOMES</topic><topic>SOMATIC CELLS</topic><topic>Subcellular Fractions - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dominguez, J.Manuel</creatorcontrib><creatorcontrib>Lanoix, J.</creatorcontrib><creatorcontrib>Paiement, Jacques</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dominguez, J.Manuel</au><au>Lanoix, J.</au><au>Paiement, Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1991</date><risdate>1991</risdate><volume>192</volume><issue>1</issue><spage>137</spage><epage>147</epage><pages>137-147</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>We have examined the antigenicity of plasma membrane (PM) and rough microsomal (RM) fractions from rat liver using anti-
ras monoclonal antibodies 142-24E05 and Y13-259 and immunochemistry as well as electron microscope immunocytochemistry. Proteins immunoprecipitated with monoclonal antibody 142-24E05 were separated using single-dimensional gradient-gel electrophoresis. The separated proteins were then blotted onto nitrocellulose sheets and incubated with [α-
32P]GTP. Radioautograms of blots indicated the presence of specific 21.5- and 22-kDa labeled proteins in the PM fraction. A 23.5-kDa [α-
32P] GTP-binding protein was detected in immunoprecipitates of both PM and RM fractions. Monoclonal antibody Y13-259 reacted only with the 21.5-kDa [α-
32P] GTP-binding protein in the plasma membrane fraction. When anti-
ras monoclonal antibody 142-24E05 and the immunogold technique were applied to membrane fractions using a preembedding immunocytochemical method, specific labeling was observed in association with both vesicular structures and membrane sheets in the PM fraction but only with electron-dense vesicular structures in the RM fraction. Thus
ras antigenicity is associated with hepatocyte plasma membranes and
ras-like antigenicity is probably associated with vesicular (secretory/endocytic) elements in both plasma membrane and rough microsomal preparations.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>1898589</pmid><doi>10.1016/0014-4827(91)90168-T</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 1991, Vol.192 (1), p.137-147 |
| issn | 0014-4827 1090-2422 |
| language | eng |
| recordid | cdi_osti_scitechconnect_6152563 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 550201 - Biochemistry- Tracer Techniques ANIMAL CELLS Animals ANTIBODIES Antibodies, Monoclonal ANTIGEN-ANTIBODY REACTIONS AUTORADIOGRAPHY BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BIOLOGICAL LOCALIZATION CELL CONSTITUENTS Cell Membrane - chemistry CELL MEMBRANES Cell membranes. Ionic channels. Membrane pores Cell structures and functions Cross Reactions DAYS LIVING RADIOISOTOPES ELECTROPHORESIS ENDOPLASMIC RETICULUM Endoplasmic Reticulum - chemistry Fundamental and applied biological sciences. Psychology GTP-Binding Proteins - immunology hepatocytes Immunohistochemistry ISOTOPES LIGHT NUCLEI Liver - chemistry LIVER CELLS MEMBRANES MICROSOMES Molecular and cellular biology Molecular Weight MONOCLONAL ANTIBODIES NUCLEI ODD-ODD NUCLEI Oncogene Protein p21(ras) - analysis ORGANIC COMPOUNDS ORGANOIDS PHOSPHORUS 32 PHOSPHORUS ISOTOPES Phosphorus Radioisotopes plasma membranes Precipitin Tests protein p21 PROTEINS RADIOISOTOPES ras Rats RIBOSOMES SOMATIC CELLS Subcellular Fractions - chemistry |
| title | Localization of ras antigenicity in rat hepatocyte plasma membrane and rough endoplasmic reticulum fractions |
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