Muscarinic and dopaminergic receptor subtypes on striatal cholinergic interneurons
Unilateral stereotaxic injection of small amounts of the cholinotoxin, AF64A, caused minimal nonselective tissue damage and resulted in a significant loss of the presynaptic cholinergic markers [ 3H]hemicholinium-3 (45% reduction) and choline acetyltransferase (27% reduction). No significant change...
Gespeichert in:
| Veröffentlicht in: | Brain research bulletin 1990-12, Vol.25 (6), p.903-912 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 912 |
|---|---|
| container_issue | 6 |
| container_start_page | 903 |
| container_title | Brain research bulletin |
| container_volume | 25 |
| creator | Dawson, Valina L. Dawson, Ted M. Wamsley, James K. |
| description | Unilateral stereotaxic injection of small amounts of the cholinotoxin, AF64A, caused minimal nonselective tissue damage and resulted in a significant loss of the presynaptic cholinergic markers [
3H]hemicholinium-3 (45% reduction) and choline acetyltransferase (27% reduction). No significant change from control was observed in tyrosine hydroxylase or tryptophan hydroxylase activity; presynaptic neuronal markers for dopamine- and serotonin-containing neurons, respectively. The AF64A lesion resulted in a significant reduction of dopamine D
2 receptors as evidenced by a decrease in [
3H]sulpiride binding (42% reduction) and decrease of muscarinic non-M
1 receptors as shown by a reduction in [
3H]QNB binding in the presence of 100 nM pirenzepine (36% reduction). Saturation studies revealed that the change in [
3H]sulpiride and [
3H]QNB binding was due to a change in B
max not K
d. Intrastriatal injection of AF64A failed to alter dopamine D
1 or muscarinic M
1 receptors labeled with [
3H]SCH23390 and[
3H]pirenzepine, respectively. In addition, no change in [
3H]forskolin-labeled adenylate cyclase was observed. These results demonstrate that a subpopulation of muscarinic receptors (non-M
1) are presynaptic on cholinergic interneurons (hence, autoreceptors), and a subpopulation of dopamine D
2 receptors are postsynaptic on cholinergic interneurons. Furthermore, dopamine D
1, muscarinic M
1 and [
3H]forskolin-labeled adenylate cyclase are not localized to striatal cholinergic interneurons. |
| doi_str_mv | 10.1016/0361-9230(90)90186-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_5962783</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0361923090901864</els_id><sourcerecordid>80267474</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-2c17284871dcaee251074cc5febc9e661347861482ccff74a9d3860f3d87c1e3</originalsourceid><addsrcrecordid>eNqFkV1rFTEQhoMo9Vj9BwqLUNGLrUk2m4-bQil-QaUgvQ85s7M2ZU-yTbJC_71ZzrG9qxAYyDzzMvO-hLxl9JRRJj_TTrLW8I5-NPSToUzLVjwjG6ZV13Il1HOyeUBeklc531JKpe7lETniTBgp1Yb8-rlkcMkHD40LQzPE2e18wPS7fiQEnEtMTV625X7G3MTQ5JK8K25q4CZO_0gfCqaAS4ohvyYvRjdlfHOox-T665fri-_t5dW3Hxfnly0IJUrLgSmuhVZsAIfIe0aVAOhH3IJBKVknlJZMaA4wjko4M3Ra0rEbtAKG3TF5v5eNuXibwReEG4ghIBTbG8mV7ir0YQ_NKd4tmIvd-Qw4TS5gXLLVlMtqlfgvyHpNNe9pBcUehBRzTjjaOfmdS_eWUbvmYlfT7Wq6NeurudhV_91Bf9nucHgYOgRR-yeHvqt5TGNyAXx-1DZScW5M5c72HFZn_3hM6-UYAAef1sOH6J9e5C8_oKmh</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15808250</pqid></control><display><type>article</type><title>Muscarinic and dopaminergic receptor subtypes on striatal cholinergic interneurons</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Dawson, Valina L. ; Dawson, Ted M. ; Wamsley, James K.</creator><creatorcontrib>Dawson, Valina L. ; Dawson, Ted M. ; Wamsley, James K.</creatorcontrib><description>Unilateral stereotaxic injection of small amounts of the cholinotoxin, AF64A, caused minimal nonselective tissue damage and resulted in a significant loss of the presynaptic cholinergic markers [
3H]hemicholinium-3 (45% reduction) and choline acetyltransferase (27% reduction). No significant change from control was observed in tyrosine hydroxylase or tryptophan hydroxylase activity; presynaptic neuronal markers for dopamine- and serotonin-containing neurons, respectively. The AF64A lesion resulted in a significant reduction of dopamine D
2 receptors as evidenced by a decrease in [
3H]sulpiride binding (42% reduction) and decrease of muscarinic non-M
1 receptors as shown by a reduction in [
3H]QNB binding in the presence of 100 nM pirenzepine (36% reduction). Saturation studies revealed that the change in [
3H]sulpiride and [
3H]QNB binding was due to a change in B
max not K
d. Intrastriatal injection of AF64A failed to alter dopamine D
1 or muscarinic M
1 receptors labeled with [
3H]SCH23390 and[
3H]pirenzepine, respectively. In addition, no change in [
3H]forskolin-labeled adenylate cyclase was observed. These results demonstrate that a subpopulation of muscarinic receptors (non-M
1) are presynaptic on cholinergic interneurons (hence, autoreceptors), and a subpopulation of dopamine D
2 receptors are postsynaptic on cholinergic interneurons. Furthermore, dopamine D
1, muscarinic M
1 and [
3H]forskolin-labeled adenylate cyclase are not localized to striatal cholinergic interneurons.</description><identifier>ISSN: 0361-9230</identifier><identifier>EISSN: 1873-2747</identifier><identifier>DOI: 10.1016/0361-9230(90)90186-4</identifier><identifier>PMID: 2149667</identifier><identifier>CODEN: BRBUDU</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>550201 - Biochemistry- Tracer Techniques ; Acetylcholine ; AF64A ; ALCOHOLS ; AMINES ; AMMONIUM COMPOUNDS ; ANIMALS ; ANTIGENS ; AROMATICS ; AUTONOMIC NERVOUS SYSTEM AGENTS ; Autoreceptors ; Aziridines - pharmacology ; BASIC BIOLOGICAL SCIENCES ; BIOCHEMICAL REACTION KINETICS ; Biological and medical sciences ; BIOLOGICAL LOCALIZATION ; BIOLOGICAL MARKERS ; BODY ; BRAIN ; CARDIOTONICS ; CARDIOVASCULAR AGENTS ; CENTRAL NERVOUS SYSTEM ; Central neurotransmission. Neuromudulation. Pathways and receptors ; CHOLINE ; Choline - analogs & derivatives ; Choline - pharmacology ; Choline O-Acetyltransferase - metabolism ; Cholinotoxin ; Corpus Striatum - drug effects ; Corpus Striatum - metabolism ; CYCLASES ; D 1 receptors ; D 2 receptors ; DOPAMINE ; Dopamine receptor subtypes ; DRUGS ; ENZYMES ; Fundamental and applied biological sciences. Psychology ; Hemicholinium-3 binding ; HYDROGEN COMPOUNDS ; HYDROXY COMPOUNDS ; HYDROXYLASES ; Interneurons - drug effects ; Interneurons - metabolism ; ISOTOPE APPLICATIONS ; KINETICS ; LIPOTROPIC FACTORS ; LYASES ; M 1 receptors ; Male ; MAMMALS ; MATERIALS ; MEMBRANE PROTEINS ; Muscarinic receptor subtypes ; NERVOUS SYSTEM ; Neuromuscular Blocking Agents - pharmacology ; NEUROREGULATORS ; Non-M 1 receptors ; Nucleus Accumbens - drug effects ; Nucleus Accumbens - metabolism ; Olfactory Bulb - drug effects ; Olfactory Bulb - metabolism ; OLFACTORY BULBS ; ORGANIC COMPOUNDS ; ORGANS ; OXIDOREDUCTASES ; PHENOLS ; Pirenzepine - metabolism ; POLYPHENOLS ; PROTEINS ; QUATERNARY COMPOUNDS ; Quinuclidinyl Benzilate - metabolism ; RATS ; Rats, Inbred Strains ; REACTION KINETICS ; RECEPTORS ; Receptors, Dopamine - drug effects ; Receptors, Dopamine - metabolism ; Receptors, Dopamine D1 ; Receptors, Dopamine D2 ; Receptors, Muscarinic - metabolism ; RODENTS ; Sulpiride - metabolism ; SYMPATHOMIMETICS ; TOXIC MATERIALS ; TOXINS ; TRACER TECHNIQUES ; TRANSFERASES ; Tritium ; TRITIUM COMPOUNDS ; VERTEBRATES ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research bulletin, 1990-12, Vol.25 (6), p.903-912</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-2c17284871dcaee251074cc5febc9e661347861482ccff74a9d3860f3d87c1e3</citedby><cites>FETCH-LOGICAL-c474t-2c17284871dcaee251074cc5febc9e661347861482ccff74a9d3860f3d87c1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0361-9230(90)90186-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19672299$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2149667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5962783$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Dawson, Valina L.</creatorcontrib><creatorcontrib>Dawson, Ted M.</creatorcontrib><creatorcontrib>Wamsley, James K.</creatorcontrib><title>Muscarinic and dopaminergic receptor subtypes on striatal cholinergic interneurons</title><title>Brain research bulletin</title><addtitle>Brain Res Bull</addtitle><description>Unilateral stereotaxic injection of small amounts of the cholinotoxin, AF64A, caused minimal nonselective tissue damage and resulted in a significant loss of the presynaptic cholinergic markers [
3H]hemicholinium-3 (45% reduction) and choline acetyltransferase (27% reduction). No significant change from control was observed in tyrosine hydroxylase or tryptophan hydroxylase activity; presynaptic neuronal markers for dopamine- and serotonin-containing neurons, respectively. The AF64A lesion resulted in a significant reduction of dopamine D
2 receptors as evidenced by a decrease in [
3H]sulpiride binding (42% reduction) and decrease of muscarinic non-M
1 receptors as shown by a reduction in [
3H]QNB binding in the presence of 100 nM pirenzepine (36% reduction). Saturation studies revealed that the change in [
3H]sulpiride and [
3H]QNB binding was due to a change in B
max not K
d. Intrastriatal injection of AF64A failed to alter dopamine D
1 or muscarinic M
1 receptors labeled with [
3H]SCH23390 and[
3H]pirenzepine, respectively. In addition, no change in [
3H]forskolin-labeled adenylate cyclase was observed. These results demonstrate that a subpopulation of muscarinic receptors (non-M
1) are presynaptic on cholinergic interneurons (hence, autoreceptors), and a subpopulation of dopamine D
2 receptors are postsynaptic on cholinergic interneurons. Furthermore, dopamine D
1, muscarinic M
1 and [
3H]forskolin-labeled adenylate cyclase are not localized to striatal cholinergic interneurons.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>Acetylcholine</subject><subject>AF64A</subject><subject>ALCOHOLS</subject><subject>AMINES</subject><subject>AMMONIUM COMPOUNDS</subject><subject>ANIMALS</subject><subject>ANTIGENS</subject><subject>AROMATICS</subject><subject>AUTONOMIC NERVOUS SYSTEM AGENTS</subject><subject>Autoreceptors</subject><subject>Aziridines - pharmacology</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOCHEMICAL REACTION KINETICS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL LOCALIZATION</subject><subject>BIOLOGICAL MARKERS</subject><subject>BODY</subject><subject>BRAIN</subject><subject>CARDIOTONICS</subject><subject>CARDIOVASCULAR AGENTS</subject><subject>CENTRAL NERVOUS SYSTEM</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>CHOLINE</subject><subject>Choline - analogs & derivatives</subject><subject>Choline - pharmacology</subject><subject>Choline O-Acetyltransferase - metabolism</subject><subject>Cholinotoxin</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - metabolism</subject><subject>CYCLASES</subject><subject>D 1 receptors</subject><subject>D 2 receptors</subject><subject>DOPAMINE</subject><subject>Dopamine receptor subtypes</subject><subject>DRUGS</subject><subject>ENZYMES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemicholinium-3 binding</subject><subject>HYDROGEN COMPOUNDS</subject><subject>HYDROXY COMPOUNDS</subject><subject>HYDROXYLASES</subject><subject>Interneurons - drug effects</subject><subject>Interneurons - metabolism</subject><subject>ISOTOPE APPLICATIONS</subject><subject>KINETICS</subject><subject>LIPOTROPIC FACTORS</subject><subject>LYASES</subject><subject>M 1 receptors</subject><subject>Male</subject><subject>MAMMALS</subject><subject>MATERIALS</subject><subject>MEMBRANE PROTEINS</subject><subject>Muscarinic receptor subtypes</subject><subject>NERVOUS SYSTEM</subject><subject>Neuromuscular Blocking Agents - pharmacology</subject><subject>NEUROREGULATORS</subject><subject>Non-M 1 receptors</subject><subject>Nucleus Accumbens - drug effects</subject><subject>Nucleus Accumbens - metabolism</subject><subject>Olfactory Bulb - drug effects</subject><subject>Olfactory Bulb - metabolism</subject><subject>OLFACTORY BULBS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>OXIDOREDUCTASES</subject><subject>PHENOLS</subject><subject>Pirenzepine - metabolism</subject><subject>POLYPHENOLS</subject><subject>PROTEINS</subject><subject>QUATERNARY COMPOUNDS</subject><subject>Quinuclidinyl Benzilate - metabolism</subject><subject>RATS</subject><subject>Rats, Inbred Strains</subject><subject>REACTION KINETICS</subject><subject>RECEPTORS</subject><subject>Receptors, Dopamine - drug effects</subject><subject>Receptors, Dopamine - metabolism</subject><subject>Receptors, Dopamine D1</subject><subject>Receptors, Dopamine D2</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>RODENTS</subject><subject>Sulpiride - metabolism</subject><subject>SYMPATHOMIMETICS</subject><subject>TOXIC MATERIALS</subject><subject>TOXINS</subject><subject>TRACER TECHNIQUES</subject><subject>TRANSFERASES</subject><subject>Tritium</subject><subject>TRITIUM COMPOUNDS</subject><subject>VERTEBRATES</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0361-9230</issn><issn>1873-2747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1rFTEQhoMo9Vj9BwqLUNGLrUk2m4-bQil-QaUgvQ85s7M2ZU-yTbJC_71ZzrG9qxAYyDzzMvO-hLxl9JRRJj_TTrLW8I5-NPSToUzLVjwjG6ZV13Il1HOyeUBeklc531JKpe7lETniTBgp1Yb8-rlkcMkHD40LQzPE2e18wPS7fiQEnEtMTV625X7G3MTQ5JK8K25q4CZO_0gfCqaAS4ohvyYvRjdlfHOox-T665fri-_t5dW3Hxfnly0IJUrLgSmuhVZsAIfIe0aVAOhH3IJBKVknlJZMaA4wjko4M3Ra0rEbtAKG3TF5v5eNuXibwReEG4ghIBTbG8mV7ir0YQ_NKd4tmIvd-Qw4TS5gXLLVlMtqlfgvyHpNNe9pBcUehBRzTjjaOfmdS_eWUbvmYlfT7Wq6NeurudhV_91Bf9nucHgYOgRR-yeHvqt5TGNyAXx-1DZScW5M5c72HFZn_3hM6-UYAAef1sOH6J9e5C8_oKmh</recordid><startdate>19901201</startdate><enddate>19901201</enddate><creator>Dawson, Valina L.</creator><creator>Dawson, Ted M.</creator><creator>Wamsley, James K.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19901201</creationdate><title>Muscarinic and dopaminergic receptor subtypes on striatal cholinergic interneurons</title><author>Dawson, Valina L. ; Dawson, Ted M. ; Wamsley, James K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-2c17284871dcaee251074cc5febc9e661347861482ccff74a9d3860f3d87c1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>Acetylcholine</topic><topic>AF64A</topic><topic>ALCOHOLS</topic><topic>AMINES</topic><topic>AMMONIUM COMPOUNDS</topic><topic>ANIMALS</topic><topic>ANTIGENS</topic><topic>AROMATICS</topic><topic>AUTONOMIC NERVOUS SYSTEM AGENTS</topic><topic>Autoreceptors</topic><topic>Aziridines - pharmacology</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOCHEMICAL REACTION KINETICS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL LOCALIZATION</topic><topic>BIOLOGICAL MARKERS</topic><topic>BODY</topic><topic>BRAIN</topic><topic>CARDIOTONICS</topic><topic>CARDIOVASCULAR AGENTS</topic><topic>CENTRAL NERVOUS SYSTEM</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>CHOLINE</topic><topic>Choline - analogs & derivatives</topic><topic>Choline - pharmacology</topic><topic>Choline O-Acetyltransferase - metabolism</topic><topic>Cholinotoxin</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - metabolism</topic><topic>CYCLASES</topic><topic>D 1 receptors</topic><topic>D 2 receptors</topic><topic>DOPAMINE</topic><topic>Dopamine receptor subtypes</topic><topic>DRUGS</topic><topic>ENZYMES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemicholinium-3 binding</topic><topic>HYDROGEN COMPOUNDS</topic><topic>HYDROXY COMPOUNDS</topic><topic>HYDROXYLASES</topic><topic>Interneurons - drug effects</topic><topic>Interneurons - metabolism</topic><topic>ISOTOPE APPLICATIONS</topic><topic>KINETICS</topic><topic>LIPOTROPIC FACTORS</topic><topic>LYASES</topic><topic>M 1 receptors</topic><topic>Male</topic><topic>MAMMALS</topic><topic>MATERIALS</topic><topic>MEMBRANE PROTEINS</topic><topic>Muscarinic receptor subtypes</topic><topic>NERVOUS SYSTEM</topic><topic>Neuromuscular Blocking Agents - pharmacology</topic><topic>NEUROREGULATORS</topic><topic>Non-M 1 receptors</topic><topic>Nucleus Accumbens - drug effects</topic><topic>Nucleus Accumbens - metabolism</topic><topic>Olfactory Bulb - drug effects</topic><topic>Olfactory Bulb - metabolism</topic><topic>OLFACTORY BULBS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>OXIDOREDUCTASES</topic><topic>PHENOLS</topic><topic>Pirenzepine - metabolism</topic><topic>POLYPHENOLS</topic><topic>PROTEINS</topic><topic>QUATERNARY COMPOUNDS</topic><topic>Quinuclidinyl Benzilate - metabolism</topic><topic>RATS</topic><topic>Rats, Inbred Strains</topic><topic>REACTION KINETICS</topic><topic>RECEPTORS</topic><topic>Receptors, Dopamine - drug effects</topic><topic>Receptors, Dopamine - metabolism</topic><topic>Receptors, Dopamine D1</topic><topic>Receptors, Dopamine D2</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>RODENTS</topic><topic>Sulpiride - metabolism</topic><topic>SYMPATHOMIMETICS</topic><topic>TOXIC MATERIALS</topic><topic>TOXINS</topic><topic>TRACER TECHNIQUES</topic><topic>TRANSFERASES</topic><topic>Tritium</topic><topic>TRITIUM COMPOUNDS</topic><topic>VERTEBRATES</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dawson, Valina L.</creatorcontrib><creatorcontrib>Dawson, Ted M.</creatorcontrib><creatorcontrib>Wamsley, James K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Brain research bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dawson, Valina L.</au><au>Dawson, Ted M.</au><au>Wamsley, James K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Muscarinic and dopaminergic receptor subtypes on striatal cholinergic interneurons</atitle><jtitle>Brain research bulletin</jtitle><addtitle>Brain Res Bull</addtitle><date>1990-12-01</date><risdate>1990</risdate><volume>25</volume><issue>6</issue><spage>903</spage><epage>912</epage><pages>903-912</pages><issn>0361-9230</issn><eissn>1873-2747</eissn><coden>BRBUDU</coden><abstract>Unilateral stereotaxic injection of small amounts of the cholinotoxin, AF64A, caused minimal nonselective tissue damage and resulted in a significant loss of the presynaptic cholinergic markers [
3H]hemicholinium-3 (45% reduction) and choline acetyltransferase (27% reduction). No significant change from control was observed in tyrosine hydroxylase or tryptophan hydroxylase activity; presynaptic neuronal markers for dopamine- and serotonin-containing neurons, respectively. The AF64A lesion resulted in a significant reduction of dopamine D
2 receptors as evidenced by a decrease in [
3H]sulpiride binding (42% reduction) and decrease of muscarinic non-M
1 receptors as shown by a reduction in [
3H]QNB binding in the presence of 100 nM pirenzepine (36% reduction). Saturation studies revealed that the change in [
3H]sulpiride and [
3H]QNB binding was due to a change in B
max not K
d. Intrastriatal injection of AF64A failed to alter dopamine D
1 or muscarinic M
1 receptors labeled with [
3H]SCH23390 and[
3H]pirenzepine, respectively. In addition, no change in [
3H]forskolin-labeled adenylate cyclase was observed. These results demonstrate that a subpopulation of muscarinic receptors (non-M
1) are presynaptic on cholinergic interneurons (hence, autoreceptors), and a subpopulation of dopamine D
2 receptors are postsynaptic on cholinergic interneurons. Furthermore, dopamine D
1, muscarinic M
1 and [
3H]forskolin-labeled adenylate cyclase are not localized to striatal cholinergic interneurons.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>2149667</pmid><doi>10.1016/0361-9230(90)90186-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0361-9230 |
| ispartof | Brain research bulletin, 1990-12, Vol.25 (6), p.903-912 |
| issn | 0361-9230 1873-2747 |
| language | eng |
| recordid | cdi_osti_scitechconnect_5962783 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 550201 - Biochemistry- Tracer Techniques Acetylcholine AF64A ALCOHOLS AMINES AMMONIUM COMPOUNDS ANIMALS ANTIGENS AROMATICS AUTONOMIC NERVOUS SYSTEM AGENTS Autoreceptors Aziridines - pharmacology BASIC BIOLOGICAL SCIENCES BIOCHEMICAL REACTION KINETICS Biological and medical sciences BIOLOGICAL LOCALIZATION BIOLOGICAL MARKERS BODY BRAIN CARDIOTONICS CARDIOVASCULAR AGENTS CENTRAL NERVOUS SYSTEM Central neurotransmission. Neuromudulation. Pathways and receptors CHOLINE Choline - analogs & derivatives Choline - pharmacology Choline O-Acetyltransferase - metabolism Cholinotoxin Corpus Striatum - drug effects Corpus Striatum - metabolism CYCLASES D 1 receptors D 2 receptors DOPAMINE Dopamine receptor subtypes DRUGS ENZYMES Fundamental and applied biological sciences. Psychology Hemicholinium-3 binding HYDROGEN COMPOUNDS HYDROXY COMPOUNDS HYDROXYLASES Interneurons - drug effects Interneurons - metabolism ISOTOPE APPLICATIONS KINETICS LIPOTROPIC FACTORS LYASES M 1 receptors Male MAMMALS MATERIALS MEMBRANE PROTEINS Muscarinic receptor subtypes NERVOUS SYSTEM Neuromuscular Blocking Agents - pharmacology NEUROREGULATORS Non-M 1 receptors Nucleus Accumbens - drug effects Nucleus Accumbens - metabolism Olfactory Bulb - drug effects Olfactory Bulb - metabolism OLFACTORY BULBS ORGANIC COMPOUNDS ORGANS OXIDOREDUCTASES PHENOLS Pirenzepine - metabolism POLYPHENOLS PROTEINS QUATERNARY COMPOUNDS Quinuclidinyl Benzilate - metabolism RATS Rats, Inbred Strains REACTION KINETICS RECEPTORS Receptors, Dopamine - drug effects Receptors, Dopamine - metabolism Receptors, Dopamine D1 Receptors, Dopamine D2 Receptors, Muscarinic - metabolism RODENTS Sulpiride - metabolism SYMPATHOMIMETICS TOXIC MATERIALS TOXINS TRACER TECHNIQUES TRANSFERASES Tritium TRITIUM COMPOUNDS VERTEBRATES Vertebrates: nervous system and sense organs |
| title | Muscarinic and dopaminergic receptor subtypes on striatal cholinergic interneurons |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T18%3A01%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Muscarinic%20and%20dopaminergic%20receptor%20subtypes%20on%20striatal%20cholinergic%20interneurons&rft.jtitle=Brain%20research%20bulletin&rft.au=Dawson,%20Valina%20L.&rft.date=1990-12-01&rft.volume=25&rft.issue=6&rft.spage=903&rft.epage=912&rft.pages=903-912&rft.issn=0361-9230&rft.eissn=1873-2747&rft.coden=BRBUDU&rft_id=info:doi/10.1016/0361-9230(90)90186-4&rft_dat=%3Cproquest_osti_%3E80267474%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15808250&rft_id=info:pmid/2149667&rft_els_id=0361923090901864&rfr_iscdi=true |