A microtiter-based assay for the detection of protein tyrosine kinase activity

A 96-well microtiter enzyme-linked immunosorbent assay (ELISA) for protein tyrosine kinases has been developed. This assay uses one of several substrates that are phosphorylated by tyrosine kinase, an antibody to phosphotyrosine, and a peroxidase-linked second antibody. Color development is monitore...

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Veröffentlicht in:	Analytical biochemistry 1990-11, Vol.190 (2), p.249-253
Hauptverfasser:	Cleaveland, Jeffrey S., Kiener, Peter A., Hammond, David J., Schacter, Bernice Z.
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques Adenosine Triphosphate - metabolism Alkaloids - pharmacology AMINO ACIDS Antibodies - immunology Autoanalysis BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Binding, Competitive BIOASSAY Carbazoles - pharmacology CARBOXYLIC ACIDS CHEMICAL REACTIONS DAYS LIVING RADIOISOTOPES ENZYME ACTIVITY ENZYME IMMUNOASSAY ENZYME INHIBITORS Enzyme Stability - drug effects Enzyme-Linked Immunosorbent Assay ENZYMES ErbB Receptors - physiology HYDROXY ACIDS IMMUNOASSAY Indole Alkaloids ISOTOPE APPLICATIONS ISOTOPES LIGHT NUCLEI Muramidase - metabolism NUCLEI ODD-ODD NUCLEI ORGANIC ACIDS ORGANIC COMPOUNDS Phosphoric Monoester Hydrolases - metabolism PHOSPHORUS 32 PHOSPHORUS ISOTOPES Phosphorus Radioisotopes PHOSPHORUS-GROUP TRANSFERASES PHOSPHORYLATION PHOSPHOTRANSFERASES Phosphotyrosine Protein-Tyrosine Kinases - analysis Protein-Tyrosine Kinases - immunology RADIOISOTOPES Staurosporine Substrate Specificity - drug effects TRACER TECHNIQUES TRANSFERASES TYROSINE Tyrosine - analogs & derivatives Tyrosine - immunology
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