A more sensitive and specific radioenzymatic assay for catecholamines
This modification of the catechol-O-methyltransferase (COMT) based radioenzymatic assay for norepinephrine (NE) and epinephrine (E) improves sensitivity, selectivity and eliminates many inhibitors of COMT. Prior to assay, samples are extracted into heptane with diphenylborate, then into dilute aceti...
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| Veröffentlicht in: | Life sciences (1973) 1990, Vol.47 (23), p.2143-2153 |
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| container_title | Life sciences (1973) |
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| creator | Kennedy, Brian Ziegler, Michael G. |
| description | This modification of the catechol-O-methyltransferase (COMT) based radioenzymatic assay for norepinephrine (NE) and epinephrine (E) improves sensitivity, selectivity and eliminates many inhibitors of COMT. Prior to assay, samples are extracted into heptane with diphenylborate, then into dilute acetic acid. This extraction procedure has an efficiency of 78% for NE but less than 2% for S-adenosylmethionine (SAM). The extraction procedure also excludes calcium and other COMT inhibitors present in urine, plasma and every tissue tested. This eliminates the requirement for individual standardization of tissue and urine samples. Sensitivity of the assay for NE and E is 10 and 6 pg/ml respectively in 1 ml of plasma. The intraassay coefficients of variation for NE and E are 4 and 13% and the interassay coefficients of variation for NE and E are 10 and 16% in a human plasma sample containing low catecholamine levels. The assay permits quantitation of plasma E levels that were undetectable in prior assays. |
| doi_str_mv | 10.1016/0024-3205(90)90314-H |
| format | Article |
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Prior to assay, samples are extracted into heptane with diphenylborate, then into dilute acetic acid. This extraction procedure has an efficiency of 78% for NE but less than 2% for S-adenosylmethionine (SAM). The extraction procedure also excludes calcium and other COMT inhibitors present in urine, plasma and every tissue tested. This eliminates the requirement for individual standardization of tissue and urine samples. Sensitivity of the assay for NE and E is 10 and 6 pg/ml respectively in 1 ml of plasma. The intraassay coefficients of variation for NE and E are 4 and 13% and the interassay coefficients of variation for NE and E are 10 and 16% in a human plasma sample containing low catecholamine levels. The assay permits quantitation of plasma E levels that were undetectable in prior assays.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/0024-3205(90)90314-H</identifier><identifier>PMID: 2266783</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>550201 - Biochemistry- Tracer Techniques ; ADRENAL HORMONES ; ADRENALINE ; AMINES ; Animals ; AROMATICS ; AUTONOMIC NERVOUS SYSTEM AGENTS ; BASIC BIOLOGICAL SCIENCES ; Borates - chemistry ; CARDIOTONICS ; CARDIOVASCULAR AGENTS ; Catechol O-Methyltransferase - chemistry ; CATECHOLAMINES ; Catecholamines - analysis ; DRUGS ; ENZYMES ; Epinephrine - analysis ; EXTRACTION ; Humans ; HYDROXY COMPOUNDS ; METHYL TRANSFERASES ; NEUROREGULATORS ; NORADRENALINE ; Norepinephrine - analysis ; ORGANIC COMPOUNDS ; PHENOLS ; POLYPHENOLS ; RADIOENZYMATIC ASSAY ; Rats ; Reproducibility of Results ; Scintillation Counting - methods ; SENSITIVITY ANALYSIS ; Sensitivity and Specificity ; SEPARATION PROCESSES ; SOLVENT EXTRACTION ; SYMPATHOMIMETICS ; TRANSFERASES</subject><ispartof>Life sciences (1973), 1990, Vol.47 (23), p.2143-2153</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-57de9d07ea544d20728e34361b47a45bdcd1562df2470d41cada3295ce2450b03</citedby><cites>FETCH-LOGICAL-c384t-57de9d07ea544d20728e34361b47a45bdcd1562df2470d41cada3295ce2450b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/002432059090314H$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2266783$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5935551$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Kennedy, Brian</creatorcontrib><creatorcontrib>Ziegler, Michael G.</creatorcontrib><title>A more sensitive and specific radioenzymatic assay for catecholamines</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>This modification of the catechol-O-methyltransferase (COMT) based radioenzymatic assay for norepinephrine (NE) and epinephrine (E) improves sensitivity, selectivity and eliminates many inhibitors of COMT. Prior to assay, samples are extracted into heptane with diphenylborate, then into dilute acetic acid. This extraction procedure has an efficiency of 78% for NE but less than 2% for S-adenosylmethionine (SAM). The extraction procedure also excludes calcium and other COMT inhibitors present in urine, plasma and every tissue tested. This eliminates the requirement for individual standardization of tissue and urine samples. Sensitivity of the assay for NE and E is 10 and 6 pg/ml respectively in 1 ml of plasma. The intraassay coefficients of variation for NE and E are 4 and 13% and the interassay coefficients of variation for NE and E are 10 and 16% in a human plasma sample containing low catecholamine levels. The assay permits quantitation of plasma E levels that were undetectable in prior assays.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>ADRENAL HORMONES</subject><subject>ADRENALINE</subject><subject>AMINES</subject><subject>Animals</subject><subject>AROMATICS</subject><subject>AUTONOMIC NERVOUS SYSTEM AGENTS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Borates - chemistry</subject><subject>CARDIOTONICS</subject><subject>CARDIOVASCULAR AGENTS</subject><subject>Catechol O-Methyltransferase - chemistry</subject><subject>CATECHOLAMINES</subject><subject>Catecholamines - analysis</subject><subject>DRUGS</subject><subject>ENZYMES</subject><subject>Epinephrine - analysis</subject><subject>EXTRACTION</subject><subject>Humans</subject><subject>HYDROXY COMPOUNDS</subject><subject>METHYL TRANSFERASES</subject><subject>NEUROREGULATORS</subject><subject>NORADRENALINE</subject><subject>Norepinephrine - analysis</subject><subject>ORGANIC COMPOUNDS</subject><subject>PHENOLS</subject><subject>POLYPHENOLS</subject><subject>RADIOENZYMATIC ASSAY</subject><subject>Rats</subject><subject>Reproducibility of Results</subject><subject>Scintillation Counting - methods</subject><subject>SENSITIVITY ANALYSIS</subject><subject>Sensitivity and Specificity</subject><subject>SEPARATION PROCESSES</subject><subject>SOLVENT EXTRACTION</subject><subject>SYMPATHOMIMETICS</subject><subject>TRANSFERASES</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotVb_gcLiQfSwOvnaj4tQSrVCwYueQzaZxUh3U5Ntof56d23x6GkY5pmZl4eQSwr3FGj2AMBEyhnI2xLuSuBUpIsjMqZFXqaQcXpMxn_IKTmL8RMApMz5iIwYy7K84GMynyaND5hEbKPr3BYT3dokrtG42pkkaOs8tt-7Rnd9q2PUu6T2ITG6Q_PhV7pxLcZzclLrVcSLQ52Q96f522yRLl-fX2bTZWp4IbpU5hZLCzlqKYRlkLMCueAZrUSuhayssVRmzNZM5GAFNdpqzkppkAkJFfAJud7f9bFzKho3hDC-bdF0SpZcSkl76GYPrYP_2mDsVOOiwdVKt-g3URXAei9F2YNiD5rgYwxYq3VwjQ47RUENitXgTw3-VAnqV7Fa9GtXh_ubqkH7t3Rw2s8f93PsTWwdhiEotgatC0NO693_D34ALPeJww</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Kennedy, Brian</creator><creator>Ziegler, Michael G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1990</creationdate><title>A more sensitive and specific radioenzymatic assay for catecholamines</title><author>Kennedy, Brian ; Ziegler, Michael G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-57de9d07ea544d20728e34361b47a45bdcd1562df2470d41cada3295ce2450b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>ADRENAL HORMONES</topic><topic>ADRENALINE</topic><topic>AMINES</topic><topic>Animals</topic><topic>AROMATICS</topic><topic>AUTONOMIC NERVOUS SYSTEM AGENTS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Borates - chemistry</topic><topic>CARDIOTONICS</topic><topic>CARDIOVASCULAR AGENTS</topic><topic>Catechol O-Methyltransferase - chemistry</topic><topic>CATECHOLAMINES</topic><topic>Catecholamines - analysis</topic><topic>DRUGS</topic><topic>ENZYMES</topic><topic>Epinephrine - analysis</topic><topic>EXTRACTION</topic><topic>Humans</topic><topic>HYDROXY COMPOUNDS</topic><topic>METHYL TRANSFERASES</topic><topic>NEUROREGULATORS</topic><topic>NORADRENALINE</topic><topic>Norepinephrine - analysis</topic><topic>ORGANIC COMPOUNDS</topic><topic>PHENOLS</topic><topic>POLYPHENOLS</topic><topic>RADIOENZYMATIC ASSAY</topic><topic>Rats</topic><topic>Reproducibility of Results</topic><topic>Scintillation Counting - methods</topic><topic>SENSITIVITY ANALYSIS</topic><topic>Sensitivity and Specificity</topic><topic>SEPARATION PROCESSES</topic><topic>SOLVENT EXTRACTION</topic><topic>SYMPATHOMIMETICS</topic><topic>TRANSFERASES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kennedy, Brian</creatorcontrib><creatorcontrib>Ziegler, Michael G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kennedy, Brian</au><au>Ziegler, Michael G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A more sensitive and specific radioenzymatic assay for catecholamines</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>1990</date><risdate>1990</risdate><volume>47</volume><issue>23</issue><spage>2143</spage><epage>2153</epage><pages>2143-2153</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>This modification of the catechol-O-methyltransferase (COMT) based radioenzymatic assay for norepinephrine (NE) and epinephrine (E) improves sensitivity, selectivity and eliminates many inhibitors of COMT. Prior to assay, samples are extracted into heptane with diphenylborate, then into dilute acetic acid. This extraction procedure has an efficiency of 78% for NE but less than 2% for S-adenosylmethionine (SAM). The extraction procedure also excludes calcium and other COMT inhibitors present in urine, plasma and every tissue tested. This eliminates the requirement for individual standardization of tissue and urine samples. Sensitivity of the assay for NE and E is 10 and 6 pg/ml respectively in 1 ml of plasma. The intraassay coefficients of variation for NE and E are 4 and 13% and the interassay coefficients of variation for NE and E are 10 and 16% in a human plasma sample containing low catecholamine levels. The assay permits quantitation of plasma E levels that were undetectable in prior assays.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>2266783</pmid><doi>10.1016/0024-3205(90)90314-H</doi><tpages>11</tpages></addata></record> |
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| ispartof | Life sciences (1973), 1990, Vol.47 (23), p.2143-2153 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 550201 - Biochemistry- Tracer Techniques ADRENAL HORMONES ADRENALINE AMINES Animals AROMATICS AUTONOMIC NERVOUS SYSTEM AGENTS BASIC BIOLOGICAL SCIENCES Borates - chemistry CARDIOTONICS CARDIOVASCULAR AGENTS Catechol O-Methyltransferase - chemistry CATECHOLAMINES Catecholamines - analysis DRUGS ENZYMES Epinephrine - analysis EXTRACTION Humans HYDROXY COMPOUNDS METHYL TRANSFERASES NEUROREGULATORS NORADRENALINE Norepinephrine - analysis ORGANIC COMPOUNDS PHENOLS POLYPHENOLS RADIOENZYMATIC ASSAY Rats Reproducibility of Results Scintillation Counting - methods SENSITIVITY ANALYSIS Sensitivity and Specificity SEPARATION PROCESSES SOLVENT EXTRACTION SYMPATHOMIMETICS TRANSFERASES |
| title | A more sensitive and specific radioenzymatic assay for catecholamines |
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