Engineered Metal-Binding Proteins: Purification to Protein Folding

Proteins can use metal ions to bind substrates, to maintain structure, to effect catalysis and for allosteric control and regulation. It is demonstrated that metal recognition can be engineered into proteins for applications such as protein purification.

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Bibliographische Detailangaben
Veröffentlicht in:	Science (American Association for the Advancement of Science) 1991-06, Vol.252 (5014), p.1796-1797
Hauptverfasser:	Arnold, Frances H., Haymore, Barry L.
Format:	Artikel
Sprache:	eng
Schlagworte:	550200 - Biochemistry 560300 - Chemicals Metabolism & Toxicology AMINO ACIDS AZOLES BASIC BIOLOGICAL SCIENCES Binding Sites BIOCHEMICAL REACTION KINETICS Biological and medical sciences BIOTECHNOLOGY CARBOXYLIC ACIDS Carrier Proteins - chemical synthesis Carrier Proteins - chemistry Carrier Proteins - isolation & purification Chelates Chelation CHEMICAL BONDS Chemical engineering Chemistry COMPLEXES Copper COPPER COMPLEXES Cytochrome c Group - chemistry Cytochromes Disulfides folding FRACTIONATION Fundamental and applied biological sciences. Psychology GENETIC ENGINEERING HETEROCYCLIC ACIDS HETEROCYCLIC COMPOUNDS HISTIDINE IMIDAZOLES KINETICS LIGANDS MEMBRANE PROTEINS METABOLISM Metal ions metal-binding protein Metallurgy Metals Metals - metabolism Methods. Procedures. Technologies Models, Molecular ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS Perspective Protein binding Protein Conformation PROTEIN ENGINEERING Protein folding PROTEINS purification RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT REACTION KINETICS RECEPTORS reviews SEPARATION PROCESSES TRANSITION ELEMENT COMPLEXES use
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