Cardiac Blood-Pool Scintigraphy in Rats and Hamsters: Comparison of Five Radiopharmaceuticals and Three Pinhole Collimator Apertures
Preclinical evaluation of cardiac drugs may require evaluation of cardiac function in intact animals. To optimize the quality of radionuclide measurements of ventricular function in small animals, a comparison was made of gated blood-pool scans recorded with five blood-pool radiopharmaceuticals (99m...
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| Veröffentlicht in: | The Journal of nuclear medicine (1978) 1991-05, Vol.32 (5), p.851-855 |
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| creator | Pieri, Pierluigi Fischman, Alan J Ahmad, Marsood Moore, Richard H Callahan, Ronald J Strauss, H. William |
| description | Preclinical evaluation of cardiac drugs may require evaluation of cardiac function in intact animals. To optimize the quality of radionuclide measurements of ventricular function in small animals, a comparison was made of gated blood-pool scans recorded with five blood-pool radiopharmaceuticals (99mTc-labeled human polyclonal IgG, 99mTc-human serum albumin labeled by two methods, and red blood cells radiolabeled with 99mTc via in vivo and in vitro methods) in rats and three pinhole apertures in hamsters. The quality of the radiopharmaceuticals was evaluated by comparing count density ratios (LV/BACKGROUND and LV/LIVER) and ejection fractions recorded with each agent. The edge definition of the left ventricle and count rate performance of the 1-, 2-, and 3-mm apertures was evaluated in hamsters. In general, the images obtained with the radiolabeled cells were superior to those obtained with the labeled proteins and no significant differences between the protein preparations were detected. Left ventricular ejection fractions calculated with all five radiopharmaceuticals were not significantly different. The best quality images were obtained with the 1-mm pinhole collimator. Ejection fraction and acquisition time were inversely related to aperture size. A good compromise between resolution and sensitivity was obtained with the 2-mm pinhole collimator. |
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William</creator><creatorcontrib>Pieri, Pierluigi ; Fischman, Alan J ; Ahmad, Marsood ; Moore, Richard H ; Callahan, Ronald J ; Strauss, H. William</creatorcontrib><description>Preclinical evaluation of cardiac drugs may require evaluation of cardiac function in intact animals. To optimize the quality of radionuclide measurements of ventricular function in small animals, a comparison was made of gated blood-pool scans recorded with five blood-pool radiopharmaceuticals (99mTc-labeled human polyclonal IgG, 99mTc-human serum albumin labeled by two methods, and red blood cells radiolabeled with 99mTc via in vivo and in vitro methods) in rats and three pinhole apertures in hamsters. The quality of the radiopharmaceuticals was evaluated by comparing count density ratios (LV/BACKGROUND and LV/LIVER) and ejection fractions recorded with each agent. The edge definition of the left ventricle and count rate performance of the 1-, 2-, and 3-mm apertures was evaluated in hamsters. In general, the images obtained with the radiolabeled cells were superior to those obtained with the labeled proteins and no significant differences between the protein preparations were detected. Left ventricular ejection fractions calculated with all five radiopharmaceuticals were not significantly different. The best quality images were obtained with the 1-mm pinhole collimator. Ejection fraction and acquisition time were inversely related to aperture size. A good compromise between resolution and sensitivity was obtained with the 2-mm pinhole collimator.</description><identifier>ISSN: 0161-5505</identifier><identifier>EISSN: 1535-5667</identifier><identifier>PMID: 1850784</identifier><language>eng</language><publisher>United States: Soc Nuclear Med</publisher><subject>550601 - Medicine- Unsealed Radionuclides in Diagnostics ; ALBUMINS ; ANIMALS ; Animals, Laboratory - physiology ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; BIOLOGICAL MATERIALS ; BLOOD ; BLOOD CELLS ; BODY ; BODY FLUIDS ; CARDIOVASCULAR SYSTEM ; COLLIMATORS ; COMPARATIVE EVALUATIONS ; COUNTING TECHNIQUES ; Cricetinae ; DIAGNOSTIC TECHNIQUES ; DRUGS ; DYNAMIC FUNCTION STUDIES ; ERYTHROCYTES ; EVALUATION ; Gated Blood-Pool Imaging - instrumentation ; Gated Blood-Pool Imaging - methods ; GLOBULINS ; HAMSTERS ; HEART ; HOURS LIVING RADIOISOTOPES ; IMMUNOGLOBULINS ; INTERMEDIATE MASS NUCLEI ; INTERNAL CONVERSION RADIOISOTOPES ; ISOMERIC NUCLEI ; ISOMERIC TRANSITION ISOTOPES ; ISOTOPES ; LABELLED COMPOUNDS ; Male ; MAMMALS ; MATERIALS ; Mesocricetus ; NUCLEI ; ODD-EVEN NUCLEI ; ORGANIC COMPOUNDS ; ORGANS ; PROTEINS ; RADIOISOTOPE SCANNING ; RADIOISOTOPES ; RADIOLOGY AND NUCLEAR MEDICINE ; RADIOPHARMACEUTICALS ; RATS ; Rats, Inbred Strains ; RODENTS ; SCINTISCANNING ; Sodium Pertechnetate Tc 99m ; Stroke Volume ; Technetium ; TECHNETIUM 99 ; TECHNETIUM ISOTOPES ; Technetium Tc 99m Aggregated Albumin ; VERTEBRATES ; YEARS LIVING RADIOI</subject><ispartof>The Journal of nuclear medicine (1978), 1991-05, Vol.32 (5), p.851-855</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1850784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5767938$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Pieri, Pierluigi</creatorcontrib><creatorcontrib>Fischman, Alan J</creatorcontrib><creatorcontrib>Ahmad, Marsood</creatorcontrib><creatorcontrib>Moore, Richard H</creatorcontrib><creatorcontrib>Callahan, Ronald J</creatorcontrib><creatorcontrib>Strauss, H. William</creatorcontrib><title>Cardiac Blood-Pool Scintigraphy in Rats and Hamsters: Comparison of Five Radiopharmaceuticals and Three Pinhole Collimator Apertures</title><title>The Journal of nuclear medicine (1978)</title><addtitle>J Nucl Med</addtitle><description>Preclinical evaluation of cardiac drugs may require evaluation of cardiac function in intact animals. To optimize the quality of radionuclide measurements of ventricular function in small animals, a comparison was made of gated blood-pool scans recorded with five blood-pool radiopharmaceuticals (99mTc-labeled human polyclonal IgG, 99mTc-human serum albumin labeled by two methods, and red blood cells radiolabeled with 99mTc via in vivo and in vitro methods) in rats and three pinhole apertures in hamsters. The quality of the radiopharmaceuticals was evaluated by comparing count density ratios (LV/BACKGROUND and LV/LIVER) and ejection fractions recorded with each agent. The edge definition of the left ventricle and count rate performance of the 1-, 2-, and 3-mm apertures was evaluated in hamsters. In general, the images obtained with the radiolabeled cells were superior to those obtained with the labeled proteins and no significant differences between the protein preparations were detected. Left ventricular ejection fractions calculated with all five radiopharmaceuticals were not significantly different. The best quality images were obtained with the 1-mm pinhole collimator. Ejection fraction and acquisition time were inversely related to aperture size. A good compromise between resolution and sensitivity was obtained with the 2-mm pinhole collimator.</description><subject>550601 - Medicine- Unsealed Radionuclides in Diagnostics</subject><subject>ALBUMINS</subject><subject>ANIMALS</subject><subject>Animals, Laboratory - physiology</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BLOOD</subject><subject>BLOOD CELLS</subject><subject>BODY</subject><subject>BODY FLUIDS</subject><subject>CARDIOVASCULAR SYSTEM</subject><subject>COLLIMATORS</subject><subject>COMPARATIVE EVALUATIONS</subject><subject>COUNTING TECHNIQUES</subject><subject>Cricetinae</subject><subject>DIAGNOSTIC TECHNIQUES</subject><subject>DRUGS</subject><subject>DYNAMIC FUNCTION STUDIES</subject><subject>ERYTHROCYTES</subject><subject>EVALUATION</subject><subject>Gated Blood-Pool Imaging - instrumentation</subject><subject>Gated Blood-Pool Imaging - methods</subject><subject>GLOBULINS</subject><subject>HAMSTERS</subject><subject>HEART</subject><subject>HOURS LIVING RADIOISOTOPES</subject><subject>IMMUNOGLOBULINS</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>INTERNAL CONVERSION RADIOISOTOPES</subject><subject>ISOMERIC NUCLEI</subject><subject>ISOMERIC TRANSITION ISOTOPES</subject><subject>ISOTOPES</subject><subject>LABELLED COMPOUNDS</subject><subject>Male</subject><subject>MAMMALS</subject><subject>MATERIALS</subject><subject>Mesocricetus</subject><subject>NUCLEI</subject><subject>ODD-EVEN NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PROTEINS</subject><subject>RADIOISOTOPE SCANNING</subject><subject>RADIOISOTOPES</subject><subject>RADIOLOGY AND NUCLEAR MEDICINE</subject><subject>RADIOPHARMACEUTICALS</subject><subject>RATS</subject><subject>Rats, Inbred Strains</subject><subject>RODENTS</subject><subject>SCINTISCANNING</subject><subject>Sodium Pertechnetate Tc 99m</subject><subject>Stroke Volume</subject><subject>Technetium</subject><subject>TECHNETIUM 99</subject><subject>TECHNETIUM ISOTOPES</subject><subject>Technetium Tc 99m Aggregated Albumin</subject><subject>VERTEBRATES</subject><subject>YEARS LIVING RADIOI</subject><issn>0161-5505</issn><issn>1535-5667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0M1q3DAUBWATWqbTJI8QEF10Z5Ct0c90Nx2apBBoaJK1kK-uxxpkyZXkhuzz4DXMQFd3850D91xU64YzXnMh5IdqTRvR1JxT_qn6nPORUiqUUqtq1ShOpdqsq_e9SdYZIN99jLZ-jNGTJ3ChuEMy0_BGXCC_TcnEBEvuzZgLpvyN7OM4meRyDCT25Nb9xUVZF6fBpNEAzsWB8afU85AQyaMLQ_S4JL13oykxkd2EqcwJ81X1sV80Xp_vZfVy--N5f18__Lr7ud891EMjt6XuoGuZ7JVluBE99kpRBI6St2ioRdFxQeW2ExLaLSJjnWqhxa6zG2UBJLDL6supN-bidAZXEAaIISAUzaWQW6YW9PWEphT_zJiLHl0G9N4EjHPWivJNI9p2gTdnOHcjWj2l5a_0ps_b_i8a3GF4dQl1mMHjMtCCj2FkreZa8Yb9A-Pghn8</recordid><startdate>199105</startdate><enddate>199105</enddate><creator>Pieri, Pierluigi</creator><creator>Fischman, Alan J</creator><creator>Ahmad, Marsood</creator><creator>Moore, Richard H</creator><creator>Callahan, Ronald J</creator><creator>Strauss, H. 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William</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h179t-bcb237f8d3e46fef880ec5e752ea0de6b56079b67c29ee33b82c2ebbd48dcc7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>550601 - Medicine- Unsealed Radionuclides in Diagnostics</topic><topic>ALBUMINS</topic><topic>ANIMALS</topic><topic>Animals, Laboratory - physiology</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BLOOD</topic><topic>BLOOD CELLS</topic><topic>BODY</topic><topic>BODY FLUIDS</topic><topic>CARDIOVASCULAR SYSTEM</topic><topic>COLLIMATORS</topic><topic>COMPARATIVE EVALUATIONS</topic><topic>COUNTING TECHNIQUES</topic><topic>Cricetinae</topic><topic>DIAGNOSTIC TECHNIQUES</topic><topic>DRUGS</topic><topic>DYNAMIC FUNCTION STUDIES</topic><topic>ERYTHROCYTES</topic><topic>EVALUATION</topic><topic>Gated Blood-Pool Imaging - instrumentation</topic><topic>Gated Blood-Pool Imaging - methods</topic><topic>GLOBULINS</topic><topic>HAMSTERS</topic><topic>HEART</topic><topic>HOURS LIVING RADIOISOTOPES</topic><topic>IMMUNOGLOBULINS</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>INTERNAL CONVERSION RADIOISOTOPES</topic><topic>ISOMERIC NUCLEI</topic><topic>ISOMERIC TRANSITION ISOTOPES</topic><topic>ISOTOPES</topic><topic>LABELLED COMPOUNDS</topic><topic>Male</topic><topic>MAMMALS</topic><topic>MATERIALS</topic><topic>Mesocricetus</topic><topic>NUCLEI</topic><topic>ODD-EVEN NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PROTEINS</topic><topic>RADIOISOTOPE SCANNING</topic><topic>RADIOISOTOPES</topic><topic>RADIOLOGY AND NUCLEAR MEDICINE</topic><topic>RADIOPHARMACEUTICALS</topic><topic>RATS</topic><topic>Rats, Inbred Strains</topic><topic>RODENTS</topic><topic>SCINTISCANNING</topic><topic>Sodium Pertechnetate Tc 99m</topic><topic>Stroke Volume</topic><topic>Technetium</topic><topic>TECHNETIUM 99</topic><topic>TECHNETIUM ISOTOPES</topic><topic>Technetium Tc 99m Aggregated Albumin</topic><topic>VERTEBRATES</topic><topic>YEARS LIVING RADIOI</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pieri, Pierluigi</creatorcontrib><creatorcontrib>Fischman, Alan J</creatorcontrib><creatorcontrib>Ahmad, Marsood</creatorcontrib><creatorcontrib>Moore, Richard H</creatorcontrib><creatorcontrib>Callahan, Ronald J</creatorcontrib><creatorcontrib>Strauss, H. William</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>The Journal of nuclear medicine (1978)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pieri, Pierluigi</au><au>Fischman, Alan J</au><au>Ahmad, Marsood</au><au>Moore, Richard H</au><au>Callahan, Ronald J</au><au>Strauss, H. William</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cardiac Blood-Pool Scintigraphy in Rats and Hamsters: Comparison of Five Radiopharmaceuticals and Three Pinhole Collimator Apertures</atitle><jtitle>The Journal of nuclear medicine (1978)</jtitle><addtitle>J Nucl Med</addtitle><date>1991-05</date><risdate>1991</risdate><volume>32</volume><issue>5</issue><spage>851</spage><epage>855</epage><pages>851-855</pages><issn>0161-5505</issn><eissn>1535-5667</eissn><abstract>Preclinical evaluation of cardiac drugs may require evaluation of cardiac function in intact animals. To optimize the quality of radionuclide measurements of ventricular function in small animals, a comparison was made of gated blood-pool scans recorded with five blood-pool radiopharmaceuticals (99mTc-labeled human polyclonal IgG, 99mTc-human serum albumin labeled by two methods, and red blood cells radiolabeled with 99mTc via in vivo and in vitro methods) in rats and three pinhole apertures in hamsters. The quality of the radiopharmaceuticals was evaluated by comparing count density ratios (LV/BACKGROUND and LV/LIVER) and ejection fractions recorded with each agent. The edge definition of the left ventricle and count rate performance of the 1-, 2-, and 3-mm apertures was evaluated in hamsters. In general, the images obtained with the radiolabeled cells were superior to those obtained with the labeled proteins and no significant differences between the protein preparations were detected. Left ventricular ejection fractions calculated with all five radiopharmaceuticals were not significantly different. The best quality images were obtained with the 1-mm pinhole collimator. Ejection fraction and acquisition time were inversely related to aperture size. A good compromise between resolution and sensitivity was obtained with the 2-mm pinhole collimator.</abstract><cop>United States</cop><pub>Soc Nuclear Med</pub><pmid>1850784</pmid><tpages>5</tpages></addata></record> |
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| ispartof | The Journal of nuclear medicine (1978), 1991-05, Vol.32 (5), p.851-855 |
| issn | 0161-5505 1535-5667 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | 550601 - Medicine- Unsealed Radionuclides in Diagnostics ALBUMINS ANIMALS Animals, Laboratory - physiology BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOLOGICAL MATERIALS BLOOD BLOOD CELLS BODY BODY FLUIDS CARDIOVASCULAR SYSTEM COLLIMATORS COMPARATIVE EVALUATIONS COUNTING TECHNIQUES Cricetinae DIAGNOSTIC TECHNIQUES DRUGS DYNAMIC FUNCTION STUDIES ERYTHROCYTES EVALUATION Gated Blood-Pool Imaging - instrumentation Gated Blood-Pool Imaging - methods GLOBULINS HAMSTERS HEART HOURS LIVING RADIOISOTOPES IMMUNOGLOBULINS INTERMEDIATE MASS NUCLEI INTERNAL CONVERSION RADIOISOTOPES ISOMERIC NUCLEI ISOMERIC TRANSITION ISOTOPES ISOTOPES LABELLED COMPOUNDS Male MAMMALS MATERIALS Mesocricetus NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANS PROTEINS RADIOISOTOPE SCANNING RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE RADIOPHARMACEUTICALS RATS Rats, Inbred Strains RODENTS SCINTISCANNING Sodium Pertechnetate Tc 99m Stroke Volume Technetium TECHNETIUM 99 TECHNETIUM ISOTOPES Technetium Tc 99m Aggregated Albumin VERTEBRATES YEARS LIVING RADIOI |
| title | Cardiac Blood-Pool Scintigraphy in Rats and Hamsters: Comparison of Five Radiopharmaceuticals and Three Pinhole Collimator Apertures |
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