Resonance Raman studies on the intermediate K-590 in the photocycle of bacteriorhodopsin
Resonance Raman (RR) spectra of the intermediate K-590 in the photocycle of the retinylidene Schiff base chromophore of bacteriorhodopsin (BR) were recorded at 21{degree}C, where K has a lifetime of {approximately}2 {mu}s. An aqueous suspension of purple membranes from Halobacteria (16 {mu}M concent...
Gespeichert in:
Veröffentlicht in: | Journal of physical chemistry (1952) 1991-03, Vol.95 (5), p.1993-2001 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 2001 |
---|---|
container_issue | 5 |
container_start_page | 1993 |
container_title | Journal of physical chemistry (1952) |
container_volume | 95 |
creator | Lohrmann, R Grieger, I Stockburger, Manfred |
description | Resonance Raman (RR) spectra of the intermediate K-590 in the photocycle of the retinylidene Schiff base chromophore of bacteriorhodopsin (BR) were recorded at 21{degree}C, where K has a lifetime of {approximately}2 {mu}s. An aqueous suspension of purple membranes from Halobacteria (16 {mu}M concentration of BR) was flowed across the waist of a laser beam (514 nm) serving for both photolysis and RR excitation. The residence time of the sample in the beam waist was {approximately}2 {mu}s. To subtract the contribution of the parent BR-570, a special experiment was designed in which two spectra, one at a low degree of photolysis (mainly consisting of BR-570) and one at a significantly higher degree of photolysis (mixture of BR-570 and K-590) could be recorded simultaneously. This allowed us to perform the difference procedure with high accuracy. New vibrational features in the spectrum of K-590 were identified in the C{double bond}C stretching region. From a comparison with the RR spectra of the parent BR-570 and the subsequent product L-550 the following was concluded: During the photoinduced transition from BR-570 to K-590 a negatively charged counterion is removed from the positively charged Schiff base group. |
doi_str_mv | 10.1021/j100158a020 |
format | Article |
fullrecord | <record><control><sourceid>acs_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_5748556</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>d236618558</sourcerecordid><originalsourceid>FETCH-LOGICAL-a423t-12325912afc879e89e7c7532d0745897ea983ae18c2e1217e95705f69df7a6993</originalsourceid><addsrcrecordid>eNpt0E1rGzEQBmBRGoib5pQ_sJRAD2FTfaxWq2MwtVvikOAkkJuYamexHFsykgLNv4_cDaaHnAQzj0ajl5AzRi8Z5ezHmlHKZAeU009kwmTDaqka-plMKOW8Fq1sjsmXlNa0OCHYhDwtMQUP3mK1hC34KuWX3mGqgq_yCivnM8Yt9g4yVte11LSU_nV2q5CDfbUbrMJQ_QFboAtxFfqwS85_JUcDbBKevp8n5HH282H6q17czn9PrxY1NFzkmnHBpWYcBtspjZ1GZZUUvKeqkZ1WCLoTgKyzHBlnCrVUVA6t7gcFrdbihHwb54aUnUnWZbQrG7xHm035fCdlW9DFiGwMKUUczC66LcRXw6jZJ2f-S67o81HvIFnYDLHE49LhimyoUGL_cj0ylzL-PbQhPptWCSXNw929uZ9NF2q-WJqb4r-PHmwy6_ASfcnlwwXeAOwuhuQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Resonance Raman studies on the intermediate K-590 in the photocycle of bacteriorhodopsin</title><source>ACS Publications</source><creator>Lohrmann, R ; Grieger, I ; Stockburger, Manfred</creator><creatorcontrib>Lohrmann, R ; Grieger, I ; Stockburger, Manfred</creatorcontrib><description>Resonance Raman (RR) spectra of the intermediate K-590 in the photocycle of the retinylidene Schiff base chromophore of bacteriorhodopsin (BR) were recorded at 21{degree}C, where K has a lifetime of {approximately}2 {mu}s. An aqueous suspension of purple membranes from Halobacteria (16 {mu}M concentration of BR) was flowed across the waist of a laser beam (514 nm) serving for both photolysis and RR excitation. The residence time of the sample in the beam waist was {approximately}2 {mu}s. To subtract the contribution of the parent BR-570, a special experiment was designed in which two spectra, one at a low degree of photolysis (mainly consisting of BR-570) and one at a significantly higher degree of photolysis (mixture of BR-570 and K-590) could be recorded simultaneously. This allowed us to perform the difference procedure with high accuracy. New vibrational features in the spectrum of K-590 were identified in the C{double bond}C stretching region. From a comparison with the RR spectra of the parent BR-570 and the subsequent product L-550 the following was concluded: During the photoinduced transition from BR-570 to K-590 a negatively charged counterion is removed from the positively charged Schiff base group.</description><identifier>ISSN: 0022-3654</identifier><identifier>EISSN: 1541-5740</identifier><identifier>DOI: 10.1021/j100158a020</identifier><identifier>CODEN: JPCHAX</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-) ; 400500 - Photochemistry ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; CHEMICAL REACTIONS ; DATA ; DATA ANALYSIS ; EXPERIMENTAL DATA ; Fundamental and applied biological sciences. Psychology ; INFORMATION ; INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY ; LASER SPECTROSCOPY ; MEASURING METHODS ; MEDIUM TEMPERATURE ; NUMERICAL DATA ; PHOTOCHEMICAL REACTIONS ; PHOTOSYNTHESIS ; PHOTOSYNTHETIC BACTERIA ; Proteins ; RAMAN SPECTROSCOPY ; SOLAR ENERGY ; SPECTROSCOPY ; SYNTHESIS</subject><ispartof>Journal of physical chemistry (1952), 1991-03, Vol.95 (5), p.1993-2001</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a423t-12325912afc879e89e7c7532d0745897ea983ae18c2e1217e95705f69df7a6993</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/j100158a020$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/j100158a020$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5403739$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5748556$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Lohrmann, R</creatorcontrib><creatorcontrib>Grieger, I</creatorcontrib><creatorcontrib>Stockburger, Manfred</creatorcontrib><title>Resonance Raman studies on the intermediate K-590 in the photocycle of bacteriorhodopsin</title><title>Journal of physical chemistry (1952)</title><addtitle>J. Phys. Chem</addtitle><description>Resonance Raman (RR) spectra of the intermediate K-590 in the photocycle of the retinylidene Schiff base chromophore of bacteriorhodopsin (BR) were recorded at 21{degree}C, where K has a lifetime of {approximately}2 {mu}s. An aqueous suspension of purple membranes from Halobacteria (16 {mu}M concentration of BR) was flowed across the waist of a laser beam (514 nm) serving for both photolysis and RR excitation. The residence time of the sample in the beam waist was {approximately}2 {mu}s. To subtract the contribution of the parent BR-570, a special experiment was designed in which two spectra, one at a low degree of photolysis (mainly consisting of BR-570) and one at a significantly higher degree of photolysis (mixture of BR-570 and K-590) could be recorded simultaneously. This allowed us to perform the difference procedure with high accuracy. New vibrational features in the spectrum of K-590 were identified in the C{double bond}C stretching region. From a comparison with the RR spectra of the parent BR-570 and the subsequent product L-550 the following was concluded: During the photoinduced transition from BR-570 to K-590 a negatively charged counterion is removed from the positively charged Schiff base group.</description><subject>140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)</subject><subject>400500 - Photochemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>CHEMICAL REACTIONS</subject><subject>DATA</subject><subject>DATA ANALYSIS</subject><subject>EXPERIMENTAL DATA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>INFORMATION</subject><subject>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</subject><subject>LASER SPECTROSCOPY</subject><subject>MEASURING METHODS</subject><subject>MEDIUM TEMPERATURE</subject><subject>NUMERICAL DATA</subject><subject>PHOTOCHEMICAL REACTIONS</subject><subject>PHOTOSYNTHESIS</subject><subject>PHOTOSYNTHETIC BACTERIA</subject><subject>Proteins</subject><subject>RAMAN SPECTROSCOPY</subject><subject>SOLAR ENERGY</subject><subject>SPECTROSCOPY</subject><subject>SYNTHESIS</subject><issn>0022-3654</issn><issn>1541-5740</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNpt0E1rGzEQBmBRGoib5pQ_sJRAD2FTfaxWq2MwtVvikOAkkJuYamexHFsykgLNv4_cDaaHnAQzj0ajl5AzRi8Z5ezHmlHKZAeU009kwmTDaqka-plMKOW8Fq1sjsmXlNa0OCHYhDwtMQUP3mK1hC34KuWX3mGqgq_yCivnM8Yt9g4yVte11LSU_nV2q5CDfbUbrMJQ_QFboAtxFfqwS85_JUcDbBKevp8n5HH282H6q17czn9PrxY1NFzkmnHBpWYcBtspjZ1GZZUUvKeqkZ1WCLoTgKyzHBlnCrVUVA6t7gcFrdbihHwb54aUnUnWZbQrG7xHm035fCdlW9DFiGwMKUUczC66LcRXw6jZJ2f-S67o81HvIFnYDLHE49LhimyoUGL_cj0ylzL-PbQhPptWCSXNw929uZ9NF2q-WJqb4r-PHmwy6_ASfcnlwwXeAOwuhuQ</recordid><startdate>199103</startdate><enddate>199103</enddate><creator>Lohrmann, R</creator><creator>Grieger, I</creator><creator>Stockburger, Manfred</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>199103</creationdate><title>Resonance Raman studies on the intermediate K-590 in the photocycle of bacteriorhodopsin</title><author>Lohrmann, R ; Grieger, I ; Stockburger, Manfred</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a423t-12325912afc879e89e7c7532d0745897ea983ae18c2e1217e95705f69df7a6993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)</topic><topic>400500 - Photochemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>CHEMICAL REACTIONS</topic><topic>DATA</topic><topic>DATA ANALYSIS</topic><topic>EXPERIMENTAL DATA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>INFORMATION</topic><topic>INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY</topic><topic>LASER SPECTROSCOPY</topic><topic>MEASURING METHODS</topic><topic>MEDIUM TEMPERATURE</topic><topic>NUMERICAL DATA</topic><topic>PHOTOCHEMICAL REACTIONS</topic><topic>PHOTOSYNTHESIS</topic><topic>PHOTOSYNTHETIC BACTERIA</topic><topic>Proteins</topic><topic>RAMAN SPECTROSCOPY</topic><topic>SOLAR ENERGY</topic><topic>SPECTROSCOPY</topic><topic>SYNTHESIS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lohrmann, R</creatorcontrib><creatorcontrib>Grieger, I</creatorcontrib><creatorcontrib>Stockburger, Manfred</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Journal of physical chemistry (1952)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lohrmann, R</au><au>Grieger, I</au><au>Stockburger, Manfred</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Resonance Raman studies on the intermediate K-590 in the photocycle of bacteriorhodopsin</atitle><jtitle>Journal of physical chemistry (1952)</jtitle><addtitle>J. Phys. Chem</addtitle><date>1991-03</date><risdate>1991</risdate><volume>95</volume><issue>5</issue><spage>1993</spage><epage>2001</epage><pages>1993-2001</pages><issn>0022-3654</issn><eissn>1541-5740</eissn><coden>JPCHAX</coden><abstract>Resonance Raman (RR) spectra of the intermediate K-590 in the photocycle of the retinylidene Schiff base chromophore of bacteriorhodopsin (BR) were recorded at 21{degree}C, where K has a lifetime of {approximately}2 {mu}s. An aqueous suspension of purple membranes from Halobacteria (16 {mu}M concentration of BR) was flowed across the waist of a laser beam (514 nm) serving for both photolysis and RR excitation. The residence time of the sample in the beam waist was {approximately}2 {mu}s. To subtract the contribution of the parent BR-570, a special experiment was designed in which two spectra, one at a low degree of photolysis (mainly consisting of BR-570) and one at a significantly higher degree of photolysis (mixture of BR-570 and K-590) could be recorded simultaneously. This allowed us to perform the difference procedure with high accuracy. New vibrational features in the spectrum of K-590 were identified in the C{double bond}C stretching region. From a comparison with the RR spectra of the parent BR-570 and the subsequent product L-550 the following was concluded: During the photoinduced transition from BR-570 to K-590 a negatively charged counterion is removed from the positively charged Schiff base group.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/j100158a020</doi><tpages>9</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0022-3654 |
ispartof | Journal of physical chemistry (1952), 1991-03, Vol.95 (5), p.1993-2001 |
issn | 0022-3654 1541-5740 |
language | eng |
recordid | cdi_osti_scitechconnect_5748556 |
source | ACS Publications |
subjects | 140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-) 400500 - Photochemistry Analytical, structural and metabolic biochemistry Biological and medical sciences CHEMICAL REACTIONS DATA DATA ANALYSIS EXPERIMENTAL DATA Fundamental and applied biological sciences. Psychology INFORMATION INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY LASER SPECTROSCOPY MEASURING METHODS MEDIUM TEMPERATURE NUMERICAL DATA PHOTOCHEMICAL REACTIONS PHOTOSYNTHESIS PHOTOSYNTHETIC BACTERIA Proteins RAMAN SPECTROSCOPY SOLAR ENERGY SPECTROSCOPY SYNTHESIS |
title | Resonance Raman studies on the intermediate K-590 in the photocycle of bacteriorhodopsin |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T10%3A48%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Resonance%20Raman%20studies%20on%20the%20intermediate%20K-590%20in%20the%20photocycle%20of%20bacteriorhodopsin&rft.jtitle=Journal%20of%20physical%20chemistry%20(1952)&rft.au=Lohrmann,%20R&rft.date=1991-03&rft.volume=95&rft.issue=5&rft.spage=1993&rft.epage=2001&rft.pages=1993-2001&rft.issn=0022-3654&rft.eissn=1541-5740&rft.coden=JPCHAX&rft_id=info:doi/10.1021/j100158a020&rft_dat=%3Cacs_osti_%3Ed236618558%3C/acs_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |