Probe Mapping to Facilitate Transposon-Based DNA Sequencing

A promising strategy for DNA sequencing exploits transposons to provide mobile sites for the binding of sequencing primers. For such a strategy to be maximally effiicent, the location and orientation of the transposon must readily determined and the insertion sites should be randomly distributed. We...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1990-08, Vol.87 (16), p.6213-6217
Hauptverfasser:	Strausbaugh, Linda D., Bourke, Michael T., Sommer, Martin T., Coon, Michael E., Berg, Claire M.
Format:	Artikel
Sprache:	eng
Schlagworte:	550200 - Biochemistry Animals AUTORADIOGRAPHY BACTERIA Base composition Base Sequence BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences Biotechnology CLONING DAYS LIVING RADIOISOTOPES DNA DNA - genetics DNA HYBRIDIZATION DNA Probes DNA SEQUENCING DNA Transposable Elements DNA-CLONING Drosophila melanogaster Drosophila melanogaster - genetics Enzymes ESCHERICHIA COLI Escherichia coli - genetics EVEN-ODD NUCLEI Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Techniques Genetic vectors HYBRIDIZATION ISOTOPES Landing sites LIGHT NUCLEI Methods. Procedures. Technologies MICROORGANISMS Molecular Sequence Data MUTAGENESIS NUCLEI NUCLEIC ACIDS Nucleotide sequences Oligonucleotide Probes ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS Plasmids RADIOISOTOPES Sequencing STRUCTURAL CHEMICAL ANALYSIS SULFUR 35 SULFUR ISOTOPES Synthetic digonucleotides and genes. Sequencing THIOLS TRANSPOSONS
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