Specific receptor for endothelin in cultured rat cardiocytes

Specific binding sites for the endothelium-derived vasoconstrictor endothelin (ET) and its effect on cytosolic free Ca 2+ concentrations ([Ca 2+] i) were studied in a primary culture of cardiocytes from neonatal rats. Binding studies using 125I-labeled-porcine ET as a radioligand revealed the presen...

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Veröffentlicht in:	Biochemical and biophysical research communications 1989-05, Vol.160 (3), p.1438-1444
Hauptverfasser:	Hirata, Yukio, Fukuda, Yuka, Yoshimi, Hiroki, Emori, Toshiaki, Shichiri, Masayoshi, Marumo, Fumiaki
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques ALKALINE EARTH METAL COMPOUNDS ANIMAL CELLS ANIMAL TISSUES ANIMALS Animals, Newborn BASIC BIOLOGICAL SCIENCES Benzofurans BETA DECAY RADIOISOTOPES BIOCHEMICAL REACTION KINETICS Biological and medical sciences BIOSYNTHESIS Blood coagulation. Blood cells BODY Calcium - metabolism Calcium Channel Blockers - pharmacology CALCIUM COMPOUNDS CARDIOVASCULAR AGENTS CARDIOVASCULAR SYSTEM CELL CULTURES Cells, Cultured Cytosol - metabolism DAYS LIVING RADIOISOTOPES DRUGS ELECTRON CAPTURE RADIOISOTOPES Endothelins ENDOTHELIUM Endothelium, Vascular ENZYME INHIBITORS FLUORESCENCE Fluorescent Dyes Fundamental and applied biological sciences. Psychology Fura-2 HEART INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES Iodine Radioisotopes ISOTOPES KINETICS LUMINESCENCE MAMMALS MEMBRANE PROTEINS Molecular and cellular biology MUSCLES MYOCARDIUM Myocardium - metabolism NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANS PEPTIDES Peptides - metabolism Peptides - pharmacology Platelet PROTEINS RADIOISOTOPES RATS REACTION KINETICS RECEPTORS Receptors, Cell Surface - drug effects Receptors, Cell Surface - metabolism Receptors, Endothelin RODENTS SYNTHESIS TISSUES Vasoconstrictor Agents - pharmacology VASOCONSTRICTORS VERTEBRATES
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