Structural characterization of the N-glycans of a recombinant hepatitis B surface antigen derived from yeast

The N-glycans of purified recombinant middle surface protein (preS2+S) from hepatitis B virus, a candidate vaccine antigen expressed in a mnn9 mutant strain of Saccharomyces cerevisiae, have been characterized structurally. The glycans were released by N-glycanase treatment, isolated by size-exclusi...

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Veröffentlicht in:	Biochemistry (Easton) 1992, Vol.31 (1), p.285-295
Hauptverfasser:	Ip, Charlotte C. Yu, Miller, William J, Kubek, Dennis J, Strang, Anne Marie, Van Halbeek, Herman, Piesecki, Susan J, Alhadeff, Jack A
Format:	Artikel
Sprache:	eng
Schlagworte:	550601 - Medicine- Unsealed Radionuclides in Diagnostics Amidohydrolases Antigenic determinants, haptens, artificial antigens ANTIGENS Biological and medical sciences Carbohydrate Conformation Carbohydrate Sequence Chromatography, High Pressure Liquid Chromatography, Ion Exchange DIGESTIVE SYSTEM DISEASES DISEASES EUMYCOTA Fundamental and applied biological sciences. Psychology Fundamental immunology FUNGI GLYCOPROTEINS HEPATITIS Hepatitis B Surface Antigens - chemistry Hepatitis B Surface Antigens - genetics Hepatitis B Surface Antigens - isolation & purification Hydrogen-Ion Concentration IMMUNOLOGY MAGNETIC RESONANCE Magnetic Resonance Spectroscopy MASS SPECTROSCOPY MEMBRANE PROTEINS MICROORGANISMS Molecular immunology Molecular Sequence Data MOLECULAR STRUCTURE NUCLEAR MAGNETIC RESONANCE ORGANIC COMPOUNDS Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase PLANTS Polysaccharides - analysis pre S2/S protein Protein Precursors - chemistry Protein Precursors - genetics Protein Precursors - isolation & purification PROTEINS RADIOLOGY AND NUCLEAR MEDICINE Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - immunology RESONANCE SACCHAROMYCES SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - chemistry Saccharomyces cerevisiae - genetics Spectrometry, Mass, Fast Atom Bombardment SPECTROSCOPY Structure-Activity Relationship VACCINES Viral Envelope Proteins - chemistry Viral Envelope Proteins - immunology Viral Hepatitis Vaccines - chemistry YEASTS
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