Incorporation of dA opposite N3-ethylthymidine terminates in vitro DNA synthesis

N3-Ethylthymidine (N3-Et-dT) was site specifically incorporated into a 17-nucleotide oligomer to investigate the significance of DNA ethylation at the central hydrogen-bonding site (N3) of thymine. The 5'-(dimethoxytrityl)-protected N3-Et-dT was converted to the corresponding 3'-phosphoram...

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Veröffentlicht in:	Biochemistry (Easton) 1990-11, Vol.29 (45), p.10357-10364
Hauptverfasser:	Bhanot, Opinder S, Grevatt, Peter C, Donahue, Jean M, Gabrielides, Christine N, Solomon, Jerome J
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques ALKYLATION Bacteriophage phi X 174 - genetics Base Composition Base Sequence BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences Chemical mutagenesis CHEMICAL REACTIONS DAYS LIVING RADIOISOTOPES Deoxyadenine Nucleotides - metabolism DERIVATIZATION DNA DNA - chemical synthesis DNA REPLICATION DNA SEQUENCING Ethylnitrosourea - metabolism Hydrogen Bonding ISOTOPES LIGHT NUCLEI Medical sciences MOLECULAR BIOLOGY Molecular Sequence Data MUTAGENESIS Mutation NITROSO COMPOUNDS NUCLEI NUCLEIC ACID REPLICATION NUCLEIC ACIDS ODD-ODD NUCLEI OLIGONUCLEOTIDES ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS PHOSPHORUS 32 PHOSPHORUS ISOTOPES RADIOISOTOPES STRUCTURAL CHEMICAL ANALYSIS Templates, Genetic Thymidine - analogs & derivatives Thymidine - metabolism Toxicology
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