Ouabain-sensitive Rb + uptake in mouse eggs and preimplantation conceptuses
The results of histochemical and immunocytochemical studies have been used elsewhere to support the hypothesis that Na + K + - ATPase expression is initiated or increases dramatically in preimplantation mouse conceptuses just before they begin to cavitate. Moreover, localization of the enzyme in the...
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| creator | Van Winkle, Lon J. Campione, Allan L. |
| description | The results of histochemical and immunocytochemical studies have been used elsewhere to support the hypothesis that
Na
+
K
+
-
ATPase
expression is initiated or increases dramatically in preimplantation mouse conceptuses just before they begin to cavitate. Moreover, localization of the enzyme in the inner membrane of the mural trophoblast is thought to be involved directly in formation and maintenance of the blastocyst cavity. Presumably,
Na
+
K
+
-
ATPase
extrudes the cation, Na
+, and therefore water into the cavity. The cation transporting activity of the enzyme can be determined by measuring ouabain-sensitive Rb
+ uptake by cells. Therefore, we measured Rb
+ uptake in mouse eggs and preimplantation conceptuses at various stages of development.
86Rb
+ uptake by conceptuses increased linearly with time for at least 60 min in medium containing 0.7 m
M total Rb
+ plus K
+ in the absence or presence of 1.0 m
M ouabain, and ouabain inhibited more than 70% of
86Rb
+ uptake. The ouabain concentration at
1
2
of maximum inhibition of the ouabain-sensitive component of
86Rb
+ uptake was about 10–20 μ
M in eggs and conceptuses at all stages of preimplantation development. Moreover, ouabain-sensitive Rb
+ uptake had a twofold higher
V
max value in blastocysts than in eggs or conceptuses at earlier stages of development (i.e., ∼173 vs 70–100 fmole · conceptus
−1 · min
−1), although the total cell surface area also was probably about two times greater in blastocysts than in eggs or other conceptuses. Ouabain-sensitive Rb
+ transport in eggs and conceptuses may have occurred via a signle ouabain-sensitive Rb
+ transporter with a Hill coefficient of 1.5–1.8 (Hill plots). When it was assumed that the Hill coefficient had a value of 2.0, however, eggs and conceptuses appeared to contain at least two forms of
Na
+
K
+
-
ATPase
activity. These studies are the first to show that the cation transporting activity of
Na
+
K
+
-
ATPase
can be measured quantitatively in mammalian eggs and preimplantation conceptuses. Inclusion of this assay in experiments designed to determine how
Na
+
K
+
-
ATPasse
activity is controlled in oocytes and conceptuses should yield further insight into the role of this enzyme in oogenesis and preimplantation development. |
| doi_str_mv | 10.1016/0012-1606(91)90456-D |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_osti_scitechconnect_5464127</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>001216069190456D</els_id><sourcerecordid>80650004</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-45f8584d738c853f7f6d938d3643093e5476790bac7cdd02dc1e2bd6421e53093</originalsourceid><addsrcrecordid>eNp9kF1rFTEQhoMo9Vj9BwpBRCxlNdl8bHIjSGutWCiIgnchm8zW6NnsmmQL_nuz7qHeeTUX88zwvg9CTyl5TQmVbwihbUMlka80PdGEC9mc30M7SrRohOTf7qPdHfIQPcr5ByGEKcWO0BGVvNNK79Cn68X2NsQmQ8yhhFvAn3t8ipe52J-AQ8TjtGTAcHOTsY0ezwnCOO9tLLaEKWI3RQdzqUx-jB4Mdp_hyWEeo68X77-cXTZX1x8-nr27ahynrDRcDEoo7jumnBJs6AbpNVOeSc6IZiB4JztNeus65z1pvaPQ9l7yloJYiWP0fPs75RJMdqGA-15zRHDFCC45bbsKvdygOU2_FsjFjCE72NfkUBsZRaSoPngF-Qa6NOWcYDBzCqNNvw0lZhVtVotmtWg0NX9Fm_N69uzwf-lH8P-ONrN1_-Kwt9nZ_ZBsdCHfYUJoRTpWsbcbBlXYbYC09oGq1Ie01vFT-H-OP8YLmAM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80650004</pqid></control><display><type>article</type><title>Ouabain-sensitive Rb + uptake in mouse eggs and preimplantation conceptuses</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Van Winkle, Lon J. ; Campione, Allan L.</creator><creatorcontrib>Van Winkle, Lon J. ; Campione, Allan L.</creatorcontrib><description>The results of histochemical and immunocytochemical studies have been used elsewhere to support the hypothesis that
Na
+
K
+
-
ATPase
expression is initiated or increases dramatically in preimplantation mouse conceptuses just before they begin to cavitate. Moreover, localization of the enzyme in the inner membrane of the mural trophoblast is thought to be involved directly in formation and maintenance of the blastocyst cavity. Presumably,
Na
+
K
+
-
ATPase
extrudes the cation, Na
+, and therefore water into the cavity. The cation transporting activity of the enzyme can be determined by measuring ouabain-sensitive Rb
+ uptake by cells. Therefore, we measured Rb
+ uptake in mouse eggs and preimplantation conceptuses at various stages of development.
86Rb
+ uptake by conceptuses increased linearly with time for at least 60 min in medium containing 0.7 m
M total Rb
+ plus K
+ in the absence or presence of 1.0 m
M ouabain, and ouabain inhibited more than 70% of
86Rb
+ uptake. The ouabain concentration at
1
2
of maximum inhibition of the ouabain-sensitive component of
86Rb
+ uptake was about 10–20 μ
M in eggs and conceptuses at all stages of preimplantation development. Moreover, ouabain-sensitive Rb
+ uptake had a twofold higher
V
max value in blastocysts than in eggs or conceptuses at earlier stages of development (i.e., ∼173 vs 70–100 fmole · conceptus
−1 · min
−1), although the total cell surface area also was probably about two times greater in blastocysts than in eggs or other conceptuses. Ouabain-sensitive Rb
+ transport in eggs and conceptuses may have occurred via a signle ouabain-sensitive Rb
+ transporter with a Hill coefficient of 1.5–1.8 (Hill plots). When it was assumed that the Hill coefficient had a value of 2.0, however, eggs and conceptuses appeared to contain at least two forms of
Na
+
K
+
-
ATPase
activity. These studies are the first to show that the cation transporting activity of
Na
+
K
+
-
ATPase
can be measured quantitatively in mammalian eggs and preimplantation conceptuses. Inclusion of this assay in experiments designed to determine how
Na
+
K
+
-
ATPasse
activity is controlled in oocytes and conceptuses should yield further insight into the role of this enzyme in oogenesis and preimplantation development.</description><identifier>ISSN: 0012-1606</identifier><identifier>EISSN: 1095-564X</identifier><identifier>DOI: 10.1016/0012-1606(91)90456-D</identifier><identifier>PMID: 1647989</identifier><identifier>CODEN: DEBIAO</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>550201 - Biochemistry- Tracer Techniques ; ACID ANHYDRASES ; ALKALI METAL COMPOUNDS ; ANIMALS ; ATP-ASE ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Biological and medical sciences ; Blastocyst - drug effects ; Blastocyst - enzymology ; Blastocyst - metabolism ; CARBOHYDRATES ; CARDIAC GLYCOSIDES ; CARDIOTONICS ; CARDIOVASCULAR AGENTS ; CATIONS ; CHARGED PARTICLES ; DAYS LIVING RADIOISOTOPES ; DRUGS ; Early stages. Segmentation. Gastrulation. Neurulation ; EGGS ; ELECTRON CAPTURE RADIOISOTOPES ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic Development - drug effects ; ENZYME ACTIVITY ; ENZYMES ; Female ; Fundamental and applied biological sciences. Psychology ; GLYCOSIDES ; HYDROLASES ; INTERMEDIATE MASS NUCLEI ; IONS ; ISOMERIC TRANSITION ISOTOPES ; ISOTOPE APPLICATIONS ; ISOTOPES ; Kinetics ; MAMMALS ; MEMBRANE TRANSPORT ; MICE ; Mice, Inbred ICR ; MINUTES LIVING RADIOISOTOPES ; NUCLEI ; ODD-ODD NUCLEI ; ORGANIC COMPOUNDS ; OUABAIN ; Ouabain - pharmacology ; Ovum - drug effects ; Ovum - enzymology ; Ovum - metabolism ; PHOSPHOHYDROLASES ; PREGNANCY ; RADIOISOTOPES ; RODENTS ; RUBIDIUM 86 ; RUBIDIUM COMPOUNDS ; RUBIDIUM ISOTOP ; Rubidium Radioisotopes ; Sodium - metabolism ; SODIUM COMPOUNDS ; Sodium-Potassium-Exchanging ATPase - metabolism ; STROPHANTHINS ; TRACER TECHNIQUES ; VERTEBRATES</subject><ispartof>Developmental biology, 1991-07, Vol.146 (1), p.158-166</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-45f8584d738c853f7f6d938d3643093e5476790bac7cdd02dc1e2bd6421e53093</citedby><cites>FETCH-LOGICAL-c413t-45f8584d738c853f7f6d938d3643093e5476790bac7cdd02dc1e2bd6421e53093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0012-1606(91)90456-D$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3541,27915,27916,45986</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5598073$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1647989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5464127$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Van Winkle, Lon J.</creatorcontrib><creatorcontrib>Campione, Allan L.</creatorcontrib><title>Ouabain-sensitive Rb + uptake in mouse eggs and preimplantation conceptuses</title><title>Developmental biology</title><addtitle>Dev Biol</addtitle><description>The results of histochemical and immunocytochemical studies have been used elsewhere to support the hypothesis that
Na
+
K
+
-
ATPase
expression is initiated or increases dramatically in preimplantation mouse conceptuses just before they begin to cavitate. Moreover, localization of the enzyme in the inner membrane of the mural trophoblast is thought to be involved directly in formation and maintenance of the blastocyst cavity. Presumably,
Na
+
K
+
-
ATPase
extrudes the cation, Na
+, and therefore water into the cavity. The cation transporting activity of the enzyme can be determined by measuring ouabain-sensitive Rb
+ uptake by cells. Therefore, we measured Rb
+ uptake in mouse eggs and preimplantation conceptuses at various stages of development.
86Rb
+ uptake by conceptuses increased linearly with time for at least 60 min in medium containing 0.7 m
M total Rb
+ plus K
+ in the absence or presence of 1.0 m
M ouabain, and ouabain inhibited more than 70% of
86Rb
+ uptake. The ouabain concentration at
1
2
of maximum inhibition of the ouabain-sensitive component of
86Rb
+ uptake was about 10–20 μ
M in eggs and conceptuses at all stages of preimplantation development. Moreover, ouabain-sensitive Rb
+ uptake had a twofold higher
V
max value in blastocysts than in eggs or conceptuses at earlier stages of development (i.e., ∼173 vs 70–100 fmole · conceptus
−1 · min
−1), although the total cell surface area also was probably about two times greater in blastocysts than in eggs or other conceptuses. Ouabain-sensitive Rb
+ transport in eggs and conceptuses may have occurred via a signle ouabain-sensitive Rb
+ transporter with a Hill coefficient of 1.5–1.8 (Hill plots). When it was assumed that the Hill coefficient had a value of 2.0, however, eggs and conceptuses appeared to contain at least two forms of
Na
+
K
+
-
ATPase
activity. These studies are the first to show that the cation transporting activity of
Na
+
K
+
-
ATPase
can be measured quantitatively in mammalian eggs and preimplantation conceptuses. Inclusion of this assay in experiments designed to determine how
Na
+
K
+
-
ATPasse
activity is controlled in oocytes and conceptuses should yield further insight into the role of this enzyme in oogenesis and preimplantation development.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>ACID ANHYDRASES</subject><subject>ALKALI METAL COMPOUNDS</subject><subject>ANIMALS</subject><subject>ATP-ASE</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - enzymology</subject><subject>Blastocyst - metabolism</subject><subject>CARBOHYDRATES</subject><subject>CARDIAC GLYCOSIDES</subject><subject>CARDIOTONICS</subject><subject>CARDIOVASCULAR AGENTS</subject><subject>CATIONS</subject><subject>CHARGED PARTICLES</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>DRUGS</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>EGGS</subject><subject>ELECTRON CAPTURE RADIOISOTOPES</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic Development - drug effects</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLYCOSIDES</subject><subject>HYDROLASES</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>IONS</subject><subject>ISOMERIC TRANSITION ISOTOPES</subject><subject>ISOTOPE APPLICATIONS</subject><subject>ISOTOPES</subject><subject>Kinetics</subject><subject>MAMMALS</subject><subject>MEMBRANE TRANSPORT</subject><subject>MICE</subject><subject>Mice, Inbred ICR</subject><subject>MINUTES LIVING RADIOISOTOPES</subject><subject>NUCLEI</subject><subject>ODD-ODD NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>OUABAIN</subject><subject>Ouabain - pharmacology</subject><subject>Ovum - drug effects</subject><subject>Ovum - enzymology</subject><subject>Ovum - metabolism</subject><subject>PHOSPHOHYDROLASES</subject><subject>PREGNANCY</subject><subject>RADIOISOTOPES</subject><subject>RODENTS</subject><subject>RUBIDIUM 86</subject><subject>RUBIDIUM COMPOUNDS</subject><subject>RUBIDIUM ISOTOP</subject><subject>Rubidium Radioisotopes</subject><subject>Sodium - metabolism</subject><subject>SODIUM COMPOUNDS</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>STROPHANTHINS</subject><subject>TRACER TECHNIQUES</subject><subject>VERTEBRATES</subject><issn>0012-1606</issn><issn>1095-564X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFTEQhoMo9Vj9BwpBRCxlNdl8bHIjSGutWCiIgnchm8zW6NnsmmQL_nuz7qHeeTUX88zwvg9CTyl5TQmVbwihbUMlka80PdGEC9mc30M7SrRohOTf7qPdHfIQPcr5ByGEKcWO0BGVvNNK79Cn68X2NsQmQ8yhhFvAn3t8ipe52J-AQ8TjtGTAcHOTsY0ezwnCOO9tLLaEKWI3RQdzqUx-jB4Mdp_hyWEeo68X77-cXTZX1x8-nr27ahynrDRcDEoo7jumnBJs6AbpNVOeSc6IZiB4JztNeus65z1pvaPQ9l7yloJYiWP0fPs75RJMdqGA-15zRHDFCC45bbsKvdygOU2_FsjFjCE72NfkUBsZRaSoPngF-Qa6NOWcYDBzCqNNvw0lZhVtVotmtWg0NX9Fm_N69uzwf-lH8P-ONrN1_-Kwt9nZ_ZBsdCHfYUJoRTpWsbcbBlXYbYC09oGq1Ie01vFT-H-OP8YLmAM</recordid><startdate>19910701</startdate><enddate>19910701</enddate><creator>Van Winkle, Lon J.</creator><creator>Campione, Allan L.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19910701</creationdate><title>Ouabain-sensitive Rb + uptake in mouse eggs and preimplantation conceptuses</title><author>Van Winkle, Lon J. ; Campione, Allan L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-45f8584d738c853f7f6d938d3643093e5476790bac7cdd02dc1e2bd6421e53093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>ACID ANHYDRASES</topic><topic>ALKALI METAL COMPOUNDS</topic><topic>ANIMALS</topic><topic>ATP-ASE</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - enzymology</topic><topic>Blastocyst - metabolism</topic><topic>CARBOHYDRATES</topic><topic>CARDIAC GLYCOSIDES</topic><topic>CARDIOTONICS</topic><topic>CARDIOVASCULAR AGENTS</topic><topic>CATIONS</topic><topic>CHARGED PARTICLES</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>DRUGS</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>EGGS</topic><topic>ELECTRON CAPTURE RADIOISOTOPES</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic Development - drug effects</topic><topic>ENZYME ACTIVITY</topic><topic>ENZYMES</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLYCOSIDES</topic><topic>HYDROLASES</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>IONS</topic><topic>ISOMERIC TRANSITION ISOTOPES</topic><topic>ISOTOPE APPLICATIONS</topic><topic>ISOTOPES</topic><topic>Kinetics</topic><topic>MAMMALS</topic><topic>MEMBRANE TRANSPORT</topic><topic>MICE</topic><topic>Mice, Inbred ICR</topic><topic>MINUTES LIVING RADIOISOTOPES</topic><topic>NUCLEI</topic><topic>ODD-ODD NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>OUABAIN</topic><topic>Ouabain - pharmacology</topic><topic>Ovum - drug effects</topic><topic>Ovum - enzymology</topic><topic>Ovum - metabolism</topic><topic>PHOSPHOHYDROLASES</topic><topic>PREGNANCY</topic><topic>RADIOISOTOPES</topic><topic>RODENTS</topic><topic>RUBIDIUM 86</topic><topic>RUBIDIUM COMPOUNDS</topic><topic>RUBIDIUM ISOTOP</topic><topic>Rubidium Radioisotopes</topic><topic>Sodium - metabolism</topic><topic>SODIUM COMPOUNDS</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>STROPHANTHINS</topic><topic>TRACER TECHNIQUES</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Winkle, Lon J.</creatorcontrib><creatorcontrib>Campione, Allan L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Winkle, Lon J.</au><au>Campione, Allan L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ouabain-sensitive Rb + uptake in mouse eggs and preimplantation conceptuses</atitle><jtitle>Developmental biology</jtitle><addtitle>Dev Biol</addtitle><date>1991-07-01</date><risdate>1991</risdate><volume>146</volume><issue>1</issue><spage>158</spage><epage>166</epage><pages>158-166</pages><issn>0012-1606</issn><eissn>1095-564X</eissn><coden>DEBIAO</coden><abstract>The results of histochemical and immunocytochemical studies have been used elsewhere to support the hypothesis that
Na
+
K
+
-
ATPase
expression is initiated or increases dramatically in preimplantation mouse conceptuses just before they begin to cavitate. Moreover, localization of the enzyme in the inner membrane of the mural trophoblast is thought to be involved directly in formation and maintenance of the blastocyst cavity. Presumably,
Na
+
K
+
-
ATPase
extrudes the cation, Na
+, and therefore water into the cavity. The cation transporting activity of the enzyme can be determined by measuring ouabain-sensitive Rb
+ uptake by cells. Therefore, we measured Rb
+ uptake in mouse eggs and preimplantation conceptuses at various stages of development.
86Rb
+ uptake by conceptuses increased linearly with time for at least 60 min in medium containing 0.7 m
M total Rb
+ plus K
+ in the absence or presence of 1.0 m
M ouabain, and ouabain inhibited more than 70% of
86Rb
+ uptake. The ouabain concentration at
1
2
of maximum inhibition of the ouabain-sensitive component of
86Rb
+ uptake was about 10–20 μ
M in eggs and conceptuses at all stages of preimplantation development. Moreover, ouabain-sensitive Rb
+ uptake had a twofold higher
V
max value in blastocysts than in eggs or conceptuses at earlier stages of development (i.e., ∼173 vs 70–100 fmole · conceptus
−1 · min
−1), although the total cell surface area also was probably about two times greater in blastocysts than in eggs or other conceptuses. Ouabain-sensitive Rb
+ transport in eggs and conceptuses may have occurred via a signle ouabain-sensitive Rb
+ transporter with a Hill coefficient of 1.5–1.8 (Hill plots). When it was assumed that the Hill coefficient had a value of 2.0, however, eggs and conceptuses appeared to contain at least two forms of
Na
+
K
+
-
ATPase
activity. These studies are the first to show that the cation transporting activity of
Na
+
K
+
-
ATPase
can be measured quantitatively in mammalian eggs and preimplantation conceptuses. Inclusion of this assay in experiments designed to determine how
Na
+
K
+
-
ATPasse
activity is controlled in oocytes and conceptuses should yield further insight into the role of this enzyme in oogenesis and preimplantation development.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>1647989</pmid><doi>10.1016/0012-1606(91)90456-D</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0012-1606 |
| ispartof | Developmental biology, 1991-07, Vol.146 (1), p.158-166 |
| issn | 0012-1606 1095-564X |
| language | eng |
| recordid | cdi_osti_scitechconnect_5464127 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 550201 - Biochemistry- Tracer Techniques ACID ANHYDRASES ALKALI METAL COMPOUNDS ANIMALS ATP-ASE BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences Blastocyst - drug effects Blastocyst - enzymology Blastocyst - metabolism CARBOHYDRATES CARDIAC GLYCOSIDES CARDIOTONICS CARDIOVASCULAR AGENTS CATIONS CHARGED PARTICLES DAYS LIVING RADIOISOTOPES DRUGS Early stages. Segmentation. Gastrulation. Neurulation EGGS ELECTRON CAPTURE RADIOISOTOPES Embryology: invertebrates and vertebrates. Teratology Embryonic Development - drug effects ENZYME ACTIVITY ENZYMES Female Fundamental and applied biological sciences. Psychology GLYCOSIDES HYDROLASES INTERMEDIATE MASS NUCLEI IONS ISOMERIC TRANSITION ISOTOPES ISOTOPE APPLICATIONS ISOTOPES Kinetics MAMMALS MEMBRANE TRANSPORT MICE Mice, Inbred ICR MINUTES LIVING RADIOISOTOPES NUCLEI ODD-ODD NUCLEI ORGANIC COMPOUNDS OUABAIN Ouabain - pharmacology Ovum - drug effects Ovum - enzymology Ovum - metabolism PHOSPHOHYDROLASES PREGNANCY RADIOISOTOPES RODENTS RUBIDIUM 86 RUBIDIUM COMPOUNDS RUBIDIUM ISOTOP Rubidium Radioisotopes Sodium - metabolism SODIUM COMPOUNDS Sodium-Potassium-Exchanging ATPase - metabolism STROPHANTHINS TRACER TECHNIQUES VERTEBRATES |
| title | Ouabain-sensitive Rb + uptake in mouse eggs and preimplantation conceptuses |
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