Solubilization of a guanine nucleotide-sensitive form of the P2Y-purinergic receptor

P2Y-Purinergic receptors were solubilized from turkey erythrocyte plasma membranes with the nonionic detergent digitonin. Adenosine 5'-O-(2-[35S]thiodiphosphate) ([35S]ADP beta S) labeled a single population of soluble high affinity sites (Kd = 12.9 nM; Bmax = 4.5 pmol/mg of protein) in an equi...

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Veröffentlicht in:	Molecular pharmacology 1991-07, Vol.40 (1), p.85-92
Hauptverfasser:	Jeffs, R A, Cooper, C L, Harden, T K
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - metabolism Adenosine Diphosphate - analogs & derivatives Adenosine Diphosphate - metabolism ADP ANIMALS AUTONOMIC NERVOUS SYSTEM AGENTS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES BIOCHEMICAL REACTION KINETICS BIOLOGICAL MATERIALS BIRDS BLOOD BLOOD CELLS BODY FLUIDS CELL CONSTITUENTS CELL MEMBRANES CHROMATOGRAPHY DAYS LIVING RADIOISOTOPES Digitonin - chemistry DRUGS Erythrocyte Membrane - chemistry Erythrocyte Membrane - metabolism ERYTHROCYTES EVEN-ODD NUCLEI FOWL GEL PERMEATION CHROMATOGRAPHY GTP-Binding Proteins - metabolism Guanine Nucleotides - metabolism INHIBITION ISOTOPE APPLICATIONS ISOTOPES KINETICS LIGHT NUCLEI MATERIALS MEMBRANE PROTEINS MEMBRANES MOLECULAR WEIGHT NUCLEI NUCLEOTIDES ODD-ODD NUCLEI ORGANIC COMPOUNDS PHOSPHORUS 32 PHOSPHORUS ISOTOPES PROTEINS RADIOISOTOPES REACTION KINETICS RECEPTORS Receptors, Purinergic - isolation & purification Receptors, Purinergic - metabolism SEPARATION PROCESSES SOLUBILITY SULFUR 35 SULFUR ISOTOPES Sulfur Radioisotopes SYMPATHOMIMETICS Thionucleotides - metabolism TRACER TECHNIQUES Turkeys VERTEBRATES 550201 > Biochemistry-- Tracer Techniques
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