Modulation of the Expression of Chondroitin Sulfate Proteoglycan in Stimulated Human Monocytes
Proteoglycan biosynthesis was studied in human monocytes and monocyte-derived macrophages (MDM) after exposure to typical activators of the monocyte/macrophage system: interferon-γ (IFN-γ), lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA). By morphological examination, both monocy...
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| Veröffentlicht in: | The Journal of biological chemistry 1989-09, Vol.264 (25), p.14916-14922 |
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| creator | Uhlin-Hansen, L Eskeland, T Kolset, S O |
| description | Proteoglycan biosynthesis was studied in human monocytes and monocyte-derived macrophages (MDM) after exposure to typical activators of the monocyte/macrophage system: interferon-γ (IFN-γ), lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA). By morphological examination, both monocytes and MDM were stimulated by these activators. Treatment with IFN-γ resulted in a slight decrease in the expression of [35S]chondroitin sulfate proteoglycan (CSPG) in both monocytes and MDM, whereas LPS treatment increased the [35S]CSPG expression 1.8 and 2.2 times, respectively. PMA, in contrast, decreased the CSPG expression 0.4 times in monocytes, whereas MDM were stimulated to increase the biosynthesis 1.9 times. An increase in the sulfate density of the chondroitin sulfate chains was evident following differentiation of monocytes into MDM due to the expression of disulfated disaccharide units of the chondroitin sulfate E type (CS-E). However, monocytes exposed to PMA did also express disaccharides of the chondroitin sulfate E type. Furthermore, the expression of CS-E in MDM was increased 2 times following PMA treatment. An inactive phorbol ester, phorbol 12,13-diacetate, did not affect the expression of CS-E in either monocytes or MDM when compared with control cultures, suggesting that protein kinase C-dependent signal pathways may be involved in the regulation of sulfation of CSPG. Exposure to LPS or IFN-γ did not lead to any changes in the sulfation of the chondroitin sulfate chains. |
| doi_str_mv | 10.1016/S0021-9258(18)63789-5 |
| format | Article |
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By morphological examination, both monocytes and MDM were stimulated by these activators. Treatment with IFN-γ resulted in a slight decrease in the expression of [35S]chondroitin sulfate proteoglycan (CSPG) in both monocytes and MDM, whereas LPS treatment increased the [35S]CSPG expression 1.8 and 2.2 times, respectively. PMA, in contrast, decreased the CSPG expression 0.4 times in monocytes, whereas MDM were stimulated to increase the biosynthesis 1.9 times. An increase in the sulfate density of the chondroitin sulfate chains was evident following differentiation of monocytes into MDM due to the expression of disulfated disaccharide units of the chondroitin sulfate E type (CS-E). However, monocytes exposed to PMA did also express disaccharides of the chondroitin sulfate E type. Furthermore, the expression of CS-E in MDM was increased 2 times following PMA treatment. An inactive phorbol ester, phorbol 12,13-diacetate, did not affect the expression of CS-E in either monocytes or MDM when compared with control cultures, suggesting that protein kinase C-dependent signal pathways may be involved in the regulation of sulfation of CSPG. Exposure to LPS or IFN-γ did not lead to any changes in the sulfation of the chondroitin sulfate chains.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(18)63789-5</identifier><identifier>PMID: 2768247</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>550201 - Biochemistry- Tracer Techniques ; 560300 - Chemicals Metabolism & Toxicology ; AMINES ; Analytical, structural and metabolic biochemistry ; ANIMAL CELLS ; ANIMALS ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; BIOLOGICAL MATERIALS ; BIOLOGICAL PATHWAYS ; BIOSYNTHESIS ; BLOOD ; BLOOD CELLS ; BODY FLUIDS ; CARBOHYDRATES ; CARCINOGENS ; CELL CULTURES ; Cells, Cultured ; CHONDROITIN ; Chondroitin Sulfate Proteoglycans - biosynthesis ; Chondroitin Sulfate Proteoglycans - metabolism ; CONNECTIVE TISSUE CELLS ; DAYS LIVING RADIOISOTOPES ; ESTERS ; EVEN-ODD NUCLEI ; Fundamental and applied biological sciences. Psychology ; GLYCOPROTEINS ; GROWTH FACTORS ; Heterosides ; Humans ; INTERFERON ; ISOTOPE APPLICATIONS ; ISOTOPES ; LEUKOCYTES ; LIGHT NUCLEI ; LIPIDS ; LIPOPOLYSACCHARIDES ; LYMPHOKINES ; Macrophage Activation - drug effects ; MACROPHAGES ; Macrophages - immunology ; Macrophages - metabolism ; Macrophages - ultrastructure ; MAMMALS ; MAN ; MATERIALS ; MITOGENS ; MONOCYTES ; Monocytes - drug effects ; Monocytes - metabolism ; Monocytes - ultrastructure ; MUCOPOLYSACCHARIDES ; NUCLEI ; ORGANIC COMPOUNDS ; Other biological molecules ; OXYGEN COMPOUNDS ; PHAGOCYTES ; PHORBOL ESTERS ; Phorbol Esters - pharmacology ; POLYSACCHARIDES ; PRIMATES ; PROTEINS ; Proteoglycans - metabolism ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIOISOTOPES ; SACCHARIDES ; SOMATIC CELLS ; SULFATES ; Sulfates - metabolism ; SULFUR 35 ; SULFUR COMPOUNDS ; SULFUR ISOTOPES ; Sulfur Radioisotopes ; SYNTHESIS ; TRACER TECHNIQUES ; VERTEBRATES</subject><ispartof>The Journal of biological chemistry, 1989-09, Vol.264 (25), p.14916-14922</ispartof><rights>1989 © 1989 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c559t-1a5f6569e114153b40c6c89875c7342fbf51ff5edb8afe29fa1263d94623ee503</citedby><cites>FETCH-LOGICAL-c559t-1a5f6569e114153b40c6c89875c7342fbf51ff5edb8afe29fa1263d94623ee503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19477990$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2768247$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5345796$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Uhlin-Hansen, L</creatorcontrib><creatorcontrib>Eskeland, T</creatorcontrib><creatorcontrib>Kolset, S O</creatorcontrib><title>Modulation of the Expression of Chondroitin Sulfate Proteoglycan in Stimulated Human Monocytes</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Proteoglycan biosynthesis was studied in human monocytes and monocyte-derived macrophages (MDM) after exposure to typical activators of the monocyte/macrophage system: interferon-γ (IFN-γ), lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA). By morphological examination, both monocytes and MDM were stimulated by these activators. Treatment with IFN-γ resulted in a slight decrease in the expression of [35S]chondroitin sulfate proteoglycan (CSPG) in both monocytes and MDM, whereas LPS treatment increased the [35S]CSPG expression 1.8 and 2.2 times, respectively. PMA, in contrast, decreased the CSPG expression 0.4 times in monocytes, whereas MDM were stimulated to increase the biosynthesis 1.9 times. An increase in the sulfate density of the chondroitin sulfate chains was evident following differentiation of monocytes into MDM due to the expression of disulfated disaccharide units of the chondroitin sulfate E type (CS-E). However, monocytes exposed to PMA did also express disaccharides of the chondroitin sulfate E type. Furthermore, the expression of CS-E in MDM was increased 2 times following PMA treatment. An inactive phorbol ester, phorbol 12,13-diacetate, did not affect the expression of CS-E in either monocytes or MDM when compared with control cultures, suggesting that protein kinase C-dependent signal pathways may be involved in the regulation of sulfation of CSPG. Exposure to LPS or IFN-γ did not lead to any changes in the sulfation of the chondroitin sulfate chains.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>560300 - Chemicals Metabolism & Toxicology</subject><subject>AMINES</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BIOLOGICAL PATHWAYS</subject><subject>BIOSYNTHESIS</subject><subject>BLOOD</subject><subject>BLOOD CELLS</subject><subject>BODY FLUIDS</subject><subject>CARBOHYDRATES</subject><subject>CARCINOGENS</subject><subject>CELL CULTURES</subject><subject>Cells, Cultured</subject><subject>CHONDROITIN</subject><subject>Chondroitin Sulfate Proteoglycans - biosynthesis</subject><subject>Chondroitin Sulfate Proteoglycans - metabolism</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>ESTERS</subject><subject>EVEN-ODD NUCLEI</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GLYCOPROTEINS</subject><subject>GROWTH FACTORS</subject><subject>Heterosides</subject><subject>Humans</subject><subject>INTERFERON</subject><subject>ISOTOPE APPLICATIONS</subject><subject>ISOTOPES</subject><subject>LEUKOCYTES</subject><subject>LIGHT NUCLEI</subject><subject>LIPIDS</subject><subject>LIPOPOLYSACCHARIDES</subject><subject>LYMPHOKINES</subject><subject>Macrophage Activation - drug effects</subject><subject>MACROPHAGES</subject><subject>Macrophages - immunology</subject><subject>Macrophages - metabolism</subject><subject>Macrophages - ultrastructure</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>MATERIALS</subject><subject>MITOGENS</subject><subject>MONOCYTES</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - metabolism</subject><subject>Monocytes - ultrastructure</subject><subject>MUCOPOLYSACCHARIDES</subject><subject>NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>Other biological molecules</subject><subject>OXYGEN COMPOUNDS</subject><subject>PHAGOCYTES</subject><subject>PHORBOL ESTERS</subject><subject>Phorbol Esters - pharmacology</subject><subject>POLYSACCHARIDES</subject><subject>PRIMATES</subject><subject>PROTEINS</subject><subject>Proteoglycans - metabolism</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIOISOTOPES</subject><subject>SACCHARIDES</subject><subject>SOMATIC CELLS</subject><subject>SULFATES</subject><subject>Sulfates - metabolism</subject><subject>SULFUR 35</subject><subject>SULFUR COMPOUNDS</subject><subject>SULFUR ISOTOPES</subject><subject>Sulfur Radioisotopes</subject><subject>SYNTHESIS</subject><subject>TRACER TECHNIQUES</subject><subject>VERTEBRATES</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV-L1DAUxYMo6-zoR1gooqIP1fxtmyeRYXWFXRRWwSdDJr3ZRtpmTFLX-fam27I-mpfAub97b84JQmcEvyGYVG-vMaaklFQ0r0jzumJ1I0vxAG0IbljJBPn-EG3ukcfoNMafOB8uyQk6oXXVUF5v0I8r3069Ts6PhbdF6qA4_3MIEOOq7Do_tsG75MbieuqtTlB8CT6Bv-mPRo_FrCc3zEOgLS6mIWtXfvTmmCA-QY-s7iM8Xe8t-vbh_Ovuorz8_PHT7v1laYSQqSRa2EpUEgjhRLA9x6YyjWxqYWrGqd1bQawV0O4bbYFKqwmtWCt5RRmAwGyLni1zfUxOReMSmM74cQSTlGBc1LLK0MsFOgT_a4KY1OCigb7XI_gpKiI4ZoTXGRQLaIKPMYBVh-AGHY6KYDWHr-7CV3OyijTqLvy8ZovO1gXTfoD2vmtNO9dfrHUdje5t0KNx8d9wyetaytnN84Xr3E136wKovfOmg0HRiisqFMm_OPt5t2CQk_3tIMzGYTTQ5pbsu_XuPw_-C9urrOw</recordid><startdate>19890905</startdate><enddate>19890905</enddate><creator>Uhlin-Hansen, L</creator><creator>Eskeland, T</creator><creator>Kolset, S O</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>OTOTI</scope></search><sort><creationdate>19890905</creationdate><title>Modulation of the Expression of Chondroitin Sulfate Proteoglycan in Stimulated Human Monocytes</title><author>Uhlin-Hansen, L ; Eskeland, T ; Kolset, S O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c559t-1a5f6569e114153b40c6c89875c7342fbf51ff5edb8afe29fa1263d94623ee503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>560300 - Chemicals Metabolism & Toxicology</topic><topic>AMINES</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BIOLOGICAL PATHWAYS</topic><topic>BIOSYNTHESIS</topic><topic>BLOOD</topic><topic>BLOOD CELLS</topic><topic>BODY FLUIDS</topic><topic>CARBOHYDRATES</topic><topic>CARCINOGENS</topic><topic>CELL CULTURES</topic><topic>Cells, Cultured</topic><topic>CHONDROITIN</topic><topic>Chondroitin Sulfate Proteoglycans - biosynthesis</topic><topic>Chondroitin Sulfate Proteoglycans - metabolism</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>ESTERS</topic><topic>EVEN-ODD NUCLEI</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GLYCOPROTEINS</topic><topic>GROWTH FACTORS</topic><topic>Heterosides</topic><topic>Humans</topic><topic>INTERFERON</topic><topic>ISOTOPE APPLICATIONS</topic><topic>ISOTOPES</topic><topic>LEUKOCYTES</topic><topic>LIGHT NUCLEI</topic><topic>LIPIDS</topic><topic>LIPOPOLYSACCHARIDES</topic><topic>LYMPHOKINES</topic><topic>Macrophage Activation - drug effects</topic><topic>MACROPHAGES</topic><topic>Macrophages - immunology</topic><topic>Macrophages - metabolism</topic><topic>Macrophages - ultrastructure</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>MATERIALS</topic><topic>MITOGENS</topic><topic>MONOCYTES</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - metabolism</topic><topic>Monocytes - ultrastructure</topic><topic>MUCOPOLYSACCHARIDES</topic><topic>NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>Other biological molecules</topic><topic>OXYGEN COMPOUNDS</topic><topic>PHAGOCYTES</topic><topic>PHORBOL ESTERS</topic><topic>Phorbol Esters - pharmacology</topic><topic>POLYSACCHARIDES</topic><topic>PRIMATES</topic><topic>PROTEINS</topic><topic>Proteoglycans - metabolism</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIOISOTOPES</topic><topic>SACCHARIDES</topic><topic>SOMATIC CELLS</topic><topic>SULFATES</topic><topic>Sulfates - metabolism</topic><topic>SULFUR 35</topic><topic>SULFUR COMPOUNDS</topic><topic>SULFUR ISOTOPES</topic><topic>Sulfur Radioisotopes</topic><topic>SYNTHESIS</topic><topic>TRACER TECHNIQUES</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uhlin-Hansen, L</creatorcontrib><creatorcontrib>Eskeland, T</creatorcontrib><creatorcontrib>Kolset, S O</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>OSTI.GOV</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uhlin-Hansen, L</au><au>Eskeland, T</au><au>Kolset, S O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of the Expression of Chondroitin Sulfate Proteoglycan in Stimulated Human Monocytes</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1989-09-05</date><risdate>1989</risdate><volume>264</volume><issue>25</issue><spage>14916</spage><epage>14922</epage><pages>14916-14922</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Proteoglycan biosynthesis was studied in human monocytes and monocyte-derived macrophages (MDM) after exposure to typical activators of the monocyte/macrophage system: interferon-γ (IFN-γ), lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA). By morphological examination, both monocytes and MDM were stimulated by these activators. Treatment with IFN-γ resulted in a slight decrease in the expression of [35S]chondroitin sulfate proteoglycan (CSPG) in both monocytes and MDM, whereas LPS treatment increased the [35S]CSPG expression 1.8 and 2.2 times, respectively. PMA, in contrast, decreased the CSPG expression 0.4 times in monocytes, whereas MDM were stimulated to increase the biosynthesis 1.9 times. An increase in the sulfate density of the chondroitin sulfate chains was evident following differentiation of monocytes into MDM due to the expression of disulfated disaccharide units of the chondroitin sulfate E type (CS-E). However, monocytes exposed to PMA did also express disaccharides of the chondroitin sulfate E type. Furthermore, the expression of CS-E in MDM was increased 2 times following PMA treatment. An inactive phorbol ester, phorbol 12,13-diacetate, did not affect the expression of CS-E in either monocytes or MDM when compared with control cultures, suggesting that protein kinase C-dependent signal pathways may be involved in the regulation of sulfation of CSPG. Exposure to LPS or IFN-γ did not lead to any changes in the sulfation of the chondroitin sulfate chains.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>2768247</pmid><doi>10.1016/S0021-9258(18)63789-5</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1989-09, Vol.264 (25), p.14916-14922 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_osti_scitechconnect_5345796 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 550201 - Biochemistry- Tracer Techniques 560300 - Chemicals Metabolism & Toxicology AMINES Analytical, structural and metabolic biochemistry ANIMAL CELLS ANIMALS BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BIOLOGICAL EFFECTS BIOLOGICAL MATERIALS BIOLOGICAL PATHWAYS BIOSYNTHESIS BLOOD BLOOD CELLS BODY FLUIDS CARBOHYDRATES CARCINOGENS CELL CULTURES Cells, Cultured CHONDROITIN Chondroitin Sulfate Proteoglycans - biosynthesis Chondroitin Sulfate Proteoglycans - metabolism CONNECTIVE TISSUE CELLS DAYS LIVING RADIOISOTOPES ESTERS EVEN-ODD NUCLEI Fundamental and applied biological sciences. Psychology GLYCOPROTEINS GROWTH FACTORS Heterosides Humans INTERFERON ISOTOPE APPLICATIONS ISOTOPES LEUKOCYTES LIGHT NUCLEI LIPIDS LIPOPOLYSACCHARIDES LYMPHOKINES Macrophage Activation - drug effects MACROPHAGES Macrophages - immunology Macrophages - metabolism Macrophages - ultrastructure MAMMALS MAN MATERIALS MITOGENS MONOCYTES Monocytes - drug effects Monocytes - metabolism Monocytes - ultrastructure MUCOPOLYSACCHARIDES NUCLEI ORGANIC COMPOUNDS Other biological molecules OXYGEN COMPOUNDS PHAGOCYTES PHORBOL ESTERS Phorbol Esters - pharmacology POLYSACCHARIDES PRIMATES PROTEINS Proteoglycans - metabolism RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIOISOTOPES SACCHARIDES SOMATIC CELLS SULFATES Sulfates - metabolism SULFUR 35 SULFUR COMPOUNDS SULFUR ISOTOPES Sulfur Radioisotopes SYNTHESIS TRACER TECHNIQUES VERTEBRATES |
| title | Modulation of the Expression of Chondroitin Sulfate Proteoglycan in Stimulated Human Monocytes |
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