Influence of proliferation on DNA repair rates in liver
To test the hypothesis that the proliferative status of a mammalian cell determines the rate of removal of oxidative DNA damage, pre- and posthepatectomized livers in adult male Fisher 344 rats were irradiated in situ with 15.5 Gy of 137Cs γ-rays. At 10 and 45 min after irradiation, the livers were...
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| Veröffentlicht in: | Experimental cell research 1991-12, Vol.197 (2), p.323-325 |
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| creator | Clair, William H.St Dwarakanath, B.S. Zhang, Hong Wheeler, Kenneth T. |
| description | To test the hypothesis that the proliferative status of a mammalian cell determines the rate of removal of oxidative DNA damage, pre- and posthepatectomized livers in adult male Fisher 344 rats were irradiated
in situ with 15.5 Gy of
137Cs γ-rays. At 10 and 45 min after irradiation, the livers were removed and dissociated into single cell suspensions, and the DNA damage in the isolated quiescent or proliferative liver cells was assayed by alkaline elution. Proliferative liver cells irradiated 20–24 h or 29–31 h after hepatectomy repaired their DNA damage faster than quiescent liver cells. A corresponding increase in the accessibility of the DNA to digestion by m. nuclease was observed for the posthepatectomized liver cells. These data suggest that proliferative status is a major determinant of the rate of DNA repair in rat liver. |
| doi_str_mv | 10.1016/0014-4827(91)90440-6 |
| format | Article |
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in situ with 15.5 Gy of
137Cs γ-rays. At 10 and 45 min after irradiation, the livers were removed and dissociated into single cell suspensions, and the DNA damage in the isolated quiescent or proliferative liver cells was assayed by alkaline elution. Proliferative liver cells irradiated 20–24 h or 29–31 h after hepatectomy repaired their DNA damage faster than quiescent liver cells. A corresponding increase in the accessibility of the DNA to digestion by m. nuclease was observed for the posthepatectomized liver cells. These data suggest that proliferative status is a major determinant of the rate of DNA repair in rat liver.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(91)90440-6</identifier><identifier>PMID: 1959564</identifier><identifier>CODEN: ECREAL</identifier><language>eng</language><publisher>Orlando, FL: Elsevier Inc</publisher><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture ; ALKALI METAL ISOTOPES ; ANIMAL CELLS ; ANIMALS ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Biological and medical sciences ; BIOLOGICAL EFFECTS ; BIOLOGICAL RADIATION EFFECTS ; BIOLOGICAL RECOVERY ; BIOLOGICAL REPAIR ; Cell Division ; CELL PROLIFERATION ; Cells, Cultured ; CESIUM 137 ; CESIUM ISOTOPES ; DNA ; DNA - genetics ; DNA - isolation & purification ; DNA Damage ; DNA REPAIR ; ELECTROMAGNETIC RADIATION ; Fundamental and applied biological sciences. Psychology ; GAMMA RADIATION ; Hepatectomy ; INTERMEDIATE MASS NUCLEI ; IONIZING RADIATIONS ; ISOTOPES ; Kinetics ; Liver - cytology ; Liver - physiology ; LIVER CELLS ; Male ; MALES ; MAMMALS ; Molecular and cellular biology ; MOLECULAR BIOLOGY ; Molecular genetics ; Mutagenesis. Repair ; NUCLEI ; NUCLEIC ACIDS ; ODD-EVEN NUCLEI ; ORGANIC COMPOUNDS ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; RADIOISOTOPES ; RATS ; Rats, Inbred F344 ; REPAIR ; RODENTS ; SOMATIC CELLS ; Time Factors ; VERTEBRATES ; YEARS LIVING RADIOISOTOPES</subject><ispartof>Experimental cell research, 1991-12, Vol.197 (2), p.323-325</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c508t-7318039dc1d81881a172607a0cbfdb21852bd4a5ef279027db48f470d91cf7ab3</citedby><cites>FETCH-LOGICAL-c508t-7318039dc1d81881a172607a0cbfdb21852bd4a5ef279027db48f470d91cf7ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-4827(91)90440-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5114229$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1959564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5267879$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Clair, William H.St</creatorcontrib><creatorcontrib>Dwarakanath, B.S.</creatorcontrib><creatorcontrib>Zhang, Hong</creatorcontrib><creatorcontrib>Wheeler, Kenneth T.</creatorcontrib><title>Influence of proliferation on DNA repair rates in liver</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>To test the hypothesis that the proliferative status of a mammalian cell determines the rate of removal of oxidative DNA damage, pre- and posthepatectomized livers in adult male Fisher 344 rats were irradiated
in situ with 15.5 Gy of
137Cs γ-rays. At 10 and 45 min after irradiation, the livers were removed and dissociated into single cell suspensions, and the DNA damage in the isolated quiescent or proliferative liver cells was assayed by alkaline elution. Proliferative liver cells irradiated 20–24 h or 29–31 h after hepatectomy repaired their DNA damage faster than quiescent liver cells. A corresponding increase in the accessibility of the DNA to digestion by m. nuclease was observed for the posthepatectomized liver cells. These data suggest that proliferative status is a major determinant of the rate of DNA repair in rat liver.</description><subject>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</subject><subject>ALKALI METAL ISOTOPES</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BIOLOGICAL RECOVERY</subject><subject>BIOLOGICAL REPAIR</subject><subject>Cell Division</subject><subject>CELL PROLIFERATION</subject><subject>Cells, Cultured</subject><subject>CESIUM 137</subject><subject>CESIUM ISOTOPES</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA - isolation & purification</subject><subject>DNA Damage</subject><subject>DNA REPAIR</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAMMA RADIATION</subject><subject>Hepatectomy</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>IONIZING RADIATIONS</subject><subject>ISOTOPES</subject><subject>Kinetics</subject><subject>Liver - cytology</subject><subject>Liver - physiology</subject><subject>LIVER CELLS</subject><subject>Male</subject><subject>MALES</subject><subject>MAMMALS</subject><subject>Molecular and cellular biology</subject><subject>MOLECULAR BIOLOGY</subject><subject>Molecular genetics</subject><subject>Mutagenesis. Repair</subject><subject>NUCLEI</subject><subject>NUCLEIC ACIDS</subject><subject>ODD-EVEN NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>RADIOISOTOPES</subject><subject>RATS</subject><subject>Rats, Inbred F344</subject><subject>REPAIR</subject><subject>RODENTS</subject><subject>SOMATIC CELLS</subject><subject>Time Factors</subject><subject>VERTEBRATES</subject><subject>YEARS LIVING RADIOISOTOPES</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtr3DAQgEVpSDdp_kEKppSSHNzOaGVLuhRC3hDSS3MWsjQiKl57K3kD_feV6yW9FQQCzTevT4ydInxBwPYrAIpaKC7PNJ5rEALq9g1bIWioueD8LVu9Iu_YUc4_AUApbA_ZIepGN61YMXk_hH5Hg6NqDNU2jX0MlOwUx6Eq5-rxokq0tTFV5ZFyFYeqjy-U3rODYPtMJ_v7mD3dXP-4vKsfvt_eX1481K4BNdVyjQrW2jv0Cktvi5K3IC24LviOo2p454VtKHCpgUvfCRWEBK_RBWm79TH7uNQd8xRNdnEi9-zGYSA3mYa3UkldoM8LVOb_taM8mU3MjvreDjTuspG8AY44g2IBXRpzThTMNsWNTb8NgpmlmtmYmY0ZjeavVNOWtA_7-rtuQ_5f0mKxxD_t4zY724dkBxfzK9Yglu-Yu39bMCrCXiKleZ9ZvY9pXseP8f9z_AE2FZAg</recordid><startdate>19911201</startdate><enddate>19911201</enddate><creator>Clair, William H.St</creator><creator>Dwarakanath, B.S.</creator><creator>Zhang, Hong</creator><creator>Wheeler, Kenneth T.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19911201</creationdate><title>Influence of proliferation on DNA repair rates in liver</title><author>Clair, William H.St ; Dwarakanath, B.S. ; Zhang, Hong ; Wheeler, Kenneth T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-7318039dc1d81881a172607a0cbfdb21852bd4a5ef279027db48f470d91cf7ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture</topic><topic>ALKALI METAL ISOTOPES</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BIOLOGICAL RECOVERY</topic><topic>BIOLOGICAL REPAIR</topic><topic>Cell Division</topic><topic>CELL PROLIFERATION</topic><topic>Cells, Cultured</topic><topic>CESIUM 137</topic><topic>CESIUM ISOTOPES</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA - isolation & purification</topic><topic>DNA Damage</topic><topic>DNA REPAIR</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAMMA RADIATION</topic><topic>Hepatectomy</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>IONIZING RADIATIONS</topic><topic>ISOTOPES</topic><topic>Kinetics</topic><topic>Liver - cytology</topic><topic>Liver - physiology</topic><topic>LIVER CELLS</topic><topic>Male</topic><topic>MALES</topic><topic>MAMMALS</topic><topic>Molecular and cellular biology</topic><topic>MOLECULAR BIOLOGY</topic><topic>Molecular genetics</topic><topic>Mutagenesis. Repair</topic><topic>NUCLEI</topic><topic>NUCLEIC ACIDS</topic><topic>ODD-EVEN NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>RADIOISOTOPES</topic><topic>RATS</topic><topic>Rats, Inbred F344</topic><topic>REPAIR</topic><topic>RODENTS</topic><topic>SOMATIC CELLS</topic><topic>Time Factors</topic><topic>VERTEBRATES</topic><topic>YEARS LIVING RADIOISOTOPES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clair, William H.St</creatorcontrib><creatorcontrib>Dwarakanath, B.S.</creatorcontrib><creatorcontrib>Zhang, Hong</creatorcontrib><creatorcontrib>Wheeler, Kenneth T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clair, William H.St</au><au>Dwarakanath, B.S.</au><au>Zhang, Hong</au><au>Wheeler, Kenneth T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of proliferation on DNA repair rates in liver</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1991-12-01</date><risdate>1991</risdate><volume>197</volume><issue>2</issue><spage>323</spage><epage>325</epage><pages>323-325</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>To test the hypothesis that the proliferative status of a mammalian cell determines the rate of removal of oxidative DNA damage, pre- and posthepatectomized livers in adult male Fisher 344 rats were irradiated
in situ with 15.5 Gy of
137Cs γ-rays. At 10 and 45 min after irradiation, the livers were removed and dissociated into single cell suspensions, and the DNA damage in the isolated quiescent or proliferative liver cells was assayed by alkaline elution. Proliferative liver cells irradiated 20–24 h or 29–31 h after hepatectomy repaired their DNA damage faster than quiescent liver cells. A corresponding increase in the accessibility of the DNA to digestion by m. nuclease was observed for the posthepatectomized liver cells. These data suggest that proliferative status is a major determinant of the rate of DNA repair in rat liver.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>1959564</pmid><doi>10.1016/0014-4827(91)90440-6</doi><tpages>3</tpages></addata></record> |
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| ispartof | Experimental cell research, 1991-12, Vol.197 (2), p.323-325 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture ALKALI METAL ISOTOPES ANIMAL CELLS ANIMALS BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS BIOLOGICAL RECOVERY BIOLOGICAL REPAIR Cell Division CELL PROLIFERATION Cells, Cultured CESIUM 137 CESIUM ISOTOPES DNA DNA - genetics DNA - isolation & purification DNA Damage DNA REPAIR ELECTROMAGNETIC RADIATION Fundamental and applied biological sciences. Psychology GAMMA RADIATION Hepatectomy INTERMEDIATE MASS NUCLEI IONIZING RADIATIONS ISOTOPES Kinetics Liver - cytology Liver - physiology LIVER CELLS Male MALES MAMMALS Molecular and cellular biology MOLECULAR BIOLOGY Molecular genetics Mutagenesis. Repair NUCLEI NUCLEIC ACIDS ODD-EVEN NUCLEI ORGANIC COMPOUNDS RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RADIOISOTOPES RATS Rats, Inbred F344 REPAIR RODENTS SOMATIC CELLS Time Factors VERTEBRATES YEARS LIVING RADIOISOTOPES |
| title | Influence of proliferation on DNA repair rates in liver |
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