A thiocyanate hydrolase of Thiobacillus thioparus. A novel enzyme catalyzing the formation of carbonyl sulfide from thiocyanate

A thiocyanate hydrolase that catalyzes the first step in thiocyanate degradation was purified to homogeneity from Thiobacillus thioparus, an obligate chemolithotrophic eubacterium metabolizing thiocyanate to sulfate as an energy source. The thiocyanate hydrolase was purified 52-fold by steps involvi...

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Veröffentlicht in:	The Journal of biological chemistry 1992-05, Vol.267 (13), p.9170-9175
Hauptverfasser:	KATAYAMA, Y, NARAHARA, Y, INOUE, Y, AMANO, F, KANAGAWA, T, KURAISHI, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry ANTITHYROID DRUGS BACTERIA Biological and medical sciences BIOSYNTHESIS Blotting, Western CARBONIC ACID DERIVATIVES CATALYSIS CHALCOGENIDES characterization CHROMATOGRAPHY Chromatography, Liquid DRUGS ELECTROPHORESIS Electrophoresis, Polyacrylamide Gel ENZYME ACTIVITY ENZYMES Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology HORMONE ANTAGONISTS HYDROLASES Hydrolases - isolation & purification Hydrolases - metabolism Hydrolysis Immunochemistry Kinetics LIQUID COLUMN CHROMATOGRAPHY METABOLISM MICROORGANISMS MOLECULAR WEIGHT ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS PROTEINS purification RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT SEPARATION PROCESSES SULFIDES SULFUR COMPOUNDS Sulfur Oxides - metabolism SYNTHESIS 560300 > Chemicals Metabolism & Toxicology Thiobacillus - enzymology Thiobacillus thioparus thiocyanate hydrolase THIOCYANATES Thiocyanates - metabolism
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